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The map label for this gene is queD [H]

Identifier: 220917100

GI number: 220917100

Start: 2251997

End: 2252386

Strand: Direct

Name: queD [H]

Synonym: A2cp1_1997

Alternate gene names: 220917100

Gene position: 2251997-2252386 (Clockwise)

Preceding gene: 220917099

Following gene: 220917101

Centisome position: 44.78

GC content: 70.0

Gene sequence:

>390_bases
ATGGCGCCCATCGACTACACCGACCGCCGGCGCATGCGGCTCGACGTCGAGTTCTACTTCGCCGCCGCGCACCGCCTGCC
GCGCTACGAGGGCCCGTGCTTCCGCATGCACGGCCACAACTACCGGTTCTTCGTCGCCCTGGAGGGCGAGGTCGATCCCG
CGACCGGGATGATCGCCGACTTCGGGGACGTGAAGCGGATCGTGCAGGAGCACGTGCTCGCGCGCGTGGACCACCGCACG
CTGAACGACGTCCTCGACAACCCCACCGCCGAGAACATCGCCCGCTGGGTGTGGGAGGTGCTCGAGCCGCACCTGCCCGG
CCTGTGCGAGATCCGGCTCTACGAGATCCCGGACTCGTGCGTGACGTACCGGGGGCCGGGTGGCCGCTAG

Upstream 100 bases:

>100_bases
CCGGCGCCTGCCCGGAGTTCCCCGAGGCGTCGGTGCGCACGTTCCCCGTGCACGTCGGCGAGGGCGGGGACCTCGAAGTG
GAGCTATAACGAGGACACCG

Downstream 100 bases:

>100_bases
GCTCCGCGCCCTGCGGCCGGCCCGCCCGCGCGCCTCGGCCCCGGCCGCCGCCGAGGCGACCGCCCGCTTCCTCGACGCGC
TGGGGCTGCCGCCGGAGGTG

Product: queuosine biosynthesis protein QueD

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MAPIDYTDRRRMRLDVEFYFAAAHRLPRYEGPCFRMHGHNYRFFVALEGEVDPATGMIADFGDVKRIVQEHVLARVDHRT
LNDVLDNPTAENIARWVWEVLEPHLPGLCEIRLYEIPDSCVTYRGPGGR

Sequences:

>Translated_129_residues
MAPIDYTDRRRMRLDVEFYFAAAHRLPRYEGPCFRMHGHNYRFFVALEGEVDPATGMIADFGDVKRIVQEHVLARVDHRT
LNDVLDNPTAENIARWVWEVLEPHLPGLCEIRLYEIPDSCVTYRGPGGR
>Mature_128_residues
APIDYTDRRRMRLDVEFYFAAAHRLPRYEGPCFRMHGHNYRFFVALEGEVDPATGMIADFGDVKRIVQEHVLARVDHRTL
NDVLDNPTAENIARWVWEVLEPHLPGLCEIRLYEIPDSCVTYRGPGGR

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Homo sapiens, GI4506331, Length=127, Percent_Identity=33.8582677165354, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1789124, Length=113, Percent_Identity=41.5929203539823, Blast_Score=87, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19550074, Length=144, Percent_Identity=28.4722222222222, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24585378, Length=144, Percent_Identity=28.4722222222222, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 14999; Mature: 14868

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPIDYTDRRRMRLDVEFYFAAAHRLPRYEGPCFRMHGHNYRFFVALEGEVDPATGMIAD
CCCCCCCCCCEEEEEHHHEEHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCHHC
FGDVKRIVQEHVLARVDHRTLNDVLDNPTAENIARWVWEVLEPHLPGLCEIRLYEIPDSC
HHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCC
VTYRGPGGR
EECCCCCCC
>Mature Secondary Structure 
APIDYTDRRRMRLDVEFYFAAAHRLPRYEGPCFRMHGHNYRFFVALEGEVDPATGMIAD
CCCCCCCCCEEEEEHHHEEHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCHHC
FGDVKRIVQEHVLARVDHRTLNDVLDNPTAENIARWVWEVLEPHLPGLCEIRLYEIPDSC
HHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCC
VTYRGPGGR
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]