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The map label for this gene is hmeA [H]

Identifier: 220916901

GI number: 220916901

Start: 2007638

End: 2008414

Strand: Direct

Name: hmeA [H]

Synonym: A2cp1_1797

Alternate gene names: 220916901

Gene position: 2007638-2008414 (Clockwise)

Preceding gene: 220916900

Following gene: 220916902

Centisome position: 39.92

GC content: 75.93

Gene sequence:

>777_bases
ATGCGAAGGCTGTCCCGGAGAAGTGCGCTGCGGCTGGGCGCGGCCGCGGCCGTGGTCACCGCGACGCGGCGCGCGGACGC
GCTCGGCCCGGAGGTCGAGGACGGCGAGGGGCCCGCGTTCCGCGGAGATCCCGCGCGCCGCTGGACGATGATCGTGGACC
TGCGCCGCTGCGTGGGCTGCCAGTCGTGCACCGTCTCCTGCGCGGTGGAGAACGCGGTCCCGGCCGGCTCGTTCCGCACC
GTGGTGTCGGAGTACGAGGTGGTCCGCGGCGGCGCCGCGCGCCGCGTCATGGTACCGCGCCTGTGCAACCACTGCGAGTC
GCCCGCCTGCACCACCGTGTGCCCGACGCAGGCGACCTGCCGGCGCGCCGACGGCGTGGTGGTGGTGAACAACACCCGCT
GCGTCGGCTGCGCGGCGTGCGTGCAGGCCTGCCCGTACGAGGGGCGCTTCCTGAACCGCGAGACCGGCACGGCCGACAAG
TGCACCTGGTGCGCGCACCGGATCGATCAGGGCCTCCTCCCCGCGTGCGTCGAGACGTGCGTGGGGGGCGCGCGCGTGTT
CGGCGATCTCGCCGATCCCGGCTCCGCCGTGTCGCGCCTCGTCCGCGAGCAGCCGCTCCAGGCGCTGCAGCCGGCCATGG
GGACGAATCCCCGGGTCCGCTACGTCGCGCTCGACGCCGAGGTCGCGGCGCGGCCGGACGCGGCGCGGCCCACGCTGTGG
GACGCGGAGGACCTGGGCATCGAGGCCACCACCCGCGTGGCCGGGGAGGGCGCATGA

Upstream 100 bases:

>100_bases
GGGGCACGGACGATGTGCCGCATCGGCTGCTTGGAGCGCCAACGTTAGCTTGCCGGCCCTCGTTTCGCGCGCCCTCTGCG
GCGCGCCGGAGGAACGGTCG

Downstream 100 bases:

>100_bases
GCATCACCGAGCTCGTCCAGCCGGTCTGGCCCGTGGCCTGGAGCCTCGCCGTCCCGCAGTACTTCTTCATGACCGGCGTC
TCGGCCGCCGCGTTCCTGGT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRRLSRRSALRLGAAAAVVTATRRADALGPEVEDGEGPAFRGDPARRWTMIVDLRRCVGCQSCTVSCAVENAVPAGSFRT
VVSEYEVVRGGAARRVMVPRLCNHCESPACTTVCPTQATCRRADGVVVVNNTRCVGCAACVQACPYEGRFLNRETGTADK
CTWCAHRIDQGLLPACVETCVGGARVFGDLADPGSAVSRLVREQPLQALQPAMGTNPRVRYVALDAEVAARPDAARPTLW
DAEDLGIEATTRVAGEGA

Sequences:

>Translated_258_residues
MRRLSRRSALRLGAAAAVVTATRRADALGPEVEDGEGPAFRGDPARRWTMIVDLRRCVGCQSCTVSCAVENAVPAGSFRT
VVSEYEVVRGGAARRVMVPRLCNHCESPACTTVCPTQATCRRADGVVVVNNTRCVGCAACVQACPYEGRFLNRETGTADK
CTWCAHRIDQGLLPACVETCVGGARVFGDLADPGSAVSRLVREQPLQALQPAMGTNPRVRYVALDAEVAARPDAARPTLW
DAEDLGIEATTRVAGEGA
>Mature_258_residues
MRRLSRRSALRLGAAAAVVTATRRADALGPEVEDGEGPAFRGDPARRWTMIVDLRRCVGCQSCTVSCAVENAVPAGSFRT
VVSEYEVVRGGAARRVMVPRLCNHCESPACTTVCPTQATCRRADGVVVVNNTRCVGCAACVQACPYEGRFLNRETGTADK
CTWCAHRIDQGLLPACVETCVGGARVFGDLADPGSAVSRLVREQPLQALQPAMGTNPRVRYVALDAEVAARPDAARPTLW
DAEDLGIEATTRVAGEGA

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=181, Percent_Identity=39.2265193370166, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI226510944, Length=155, Percent_Identity=38.7096774193548, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787122, Length=201, Percent_Identity=32.8358208955224, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787872, Length=201, Percent_Identity=32.8358208955224, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787478, Length=105, Percent_Identity=39.0476190476191, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787740, Length=96, Percent_Identity=39.5833333333333, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787749, Length=155, Percent_Identity=32.9032258064516, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790326, Length=201, Percent_Identity=29.3532338308458, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1789370, Length=166, Percent_Identity=32.5301204819277, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367245, Length=164, Percent_Identity=33.5365853658537, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788811, Length=178, Percent_Identity=29.7752808988764, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787963, Length=176, Percent_Identity=26.1363636363636, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789067, Length=159, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI87082179, Length=142, Percent_Identity=32.3943661971831, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27403; Mature: 27403

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
6.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLSRRSALRLGAAAAVVTATRRADALGPEVEDGEGPAFRGDPARRWTMIVDLRRCVGC
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
QSCTVSCAVENAVPAGSFRTVVSEYEVVRGGAARRVMVPRLCNHCESPACTTVCPTQATC
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEECCCHHHH
RRADGVVVVNNTRCVGCAACVQACPYEGRFLNRETGTADKCTWCAHRIDQGLLPACVETC
HHCCCEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
VGGARVFGDLADPGSAVSRLVREQPLQALQPAMGTNPRVRYVALDAEVAARPDAARPTLW
CCHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCHHHCCCCCCCCCCC
DAEDLGIEATTRVAGEGA
CHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MRRLSRRSALRLGAAAAVVTATRRADALGPEVEDGEGPAFRGDPARRWTMIVDLRRCVGC
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
QSCTVSCAVENAVPAGSFRTVVSEYEVVRGGAARRVMVPRLCNHCESPACTTVCPTQATC
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEECCCHHHH
RRADGVVVVNNTRCVGCAACVQACPYEGRFLNRETGTADKCTWCAHRIDQGLLPACVETC
HHCCCEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
VGGARVFGDLADPGSAVSRLVREQPLQALQPAMGTNPRVRYVALDAEVAARPDAARPTLW
CCHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCHHHCCCCCCCCCCC
DAEDLGIEATTRVAGEGA
CHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]