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The map label for this gene is mdh2 [H]

Identifier: 220916826

GI number: 220916826

Start: 1921123

End: 1922073

Strand: Direct

Name: mdh2 [H]

Synonym: A2cp1_1722

Alternate gene names: 220916826

Gene position: 1921123-1922073 (Clockwise)

Preceding gene: 220916825

Following gene: 220916831

Centisome position: 38.2

GC content: 69.19

Gene sequence:

>951_bases
ATGGCCAGGTCCAAGATTGCACTGATCGGCGGTGGGCAGATCGGCGGCGTGCTGGCGCAGCTCGCGGCGCTCCGCGAGCT
GGGCGACGTGGTCCTCTTCGACATCGTCGAGGGGCTGCCGCAGGGGAAGACGCTGGACATCGCCGAGGCCGCGCCGGTCG
ACGGCTTCGACGTCAGCCTCAAGGGCACGAACACCTACGAGGACATCAAGGGCGCGGACGTCGTGATCGTCACCGCCGGC
CTGCCCCGCAAGCCGGGCATGAGCCGTGACGACCTCATCGCGGTGAACTCGAAGATCATGACGACCGTGGCCGAGGGCAT
CAAGCAGTACGCCCCGAACGCCTTCGTCATCGTGATCTCGAACCCGCTCGACGCGATGGTGACGCTCTGCCAGCGCATCA
CGGGGTTCCCGCACAGCCGCGTGGTCGGCCAGGCCGGCGTGCTCGACTCGGCCCGCTTCGCCGCGTTCATCGCGTGGGAG
CTGGGCGTCTCCGTCAAGGACGTCACCGCGGTCACCCTGGGCGGCCACGGCGACGACATGGTGCCGCTGGTCCGCTACAC
CAGCGTCTGCGGCGTCCCGGTGATGGAGCTGCTCGAGCAGAAGTACGGCGCCGCCAAGGCCGCCGAGGTGATGGCCGCGA
TGGTGAAGCGGACCCGCGGCGCGGGCGGCGAGGTGGTCGCGCTCCTCAAGACCGGCAGCGCGTTCTACTCGCCGGCCTCC
AGCGCCATCGCGATGGCAGAGTCGTTCCTGAAGGACCAGAAGCGCGTGCTCCCGACCTGCGCCTTCCTGAAGGGCGAGTT
CGGCGTGGACGGCCTCTACGTCGGCGTGCCGGTGGTGATCGGCGCGGGCGGCGCGGAGCGCGTCCTCCAGCTCAAGCTGA
ACGCCGAGGAGCAGGCCATGATGGACAAGTCCGTCAAGGCCGTGAAGGACCTGGTCGCCACGCTGAAGTAG

Upstream 100 bases:

>100_bases
AAGACCGGGAAGGCGATGCGTCCCAGCGCGACGCTGAACGCAATCATCGACGCGATGGCGTAACTGCAGGAATCCAACCA
GTCAGGCGAGGGAGACGAAC

Downstream 100 bases:

>100_bases
CGACCGCCGCGGCCTCGCCGCGGAGCAGGACCCGGGCCCCGATCGCCGCCAGGCGGTCGGGGCCGCTTCGTTCAGGGGGG
GGCGCTACGCCAGCCCGAGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MARSKIALIGGGQIGGVLAQLAALRELGDVVLFDIVEGLPQGKTLDIAEAAPVDGFDVSLKGTNTYEDIKGADVVIVTAG
LPRKPGMSRDDLIAVNSKIMTTVAEGIKQYAPNAFVIVISNPLDAMVTLCQRITGFPHSRVVGQAGVLDSARFAAFIAWE
LGVSVKDVTAVTLGGHGDDMVPLVRYTSVCGVPVMELLEQKYGAAKAAEVMAAMVKRTRGAGGEVVALLKTGSAFYSPAS
SAIAMAESFLKDQKRVLPTCAFLKGEFGVDGLYVGVPVVIGAGGAERVLQLKLNAEEQAMMDKSVKAVKDLVATLK

Sequences:

>Translated_316_residues
MARSKIALIGGGQIGGVLAQLAALRELGDVVLFDIVEGLPQGKTLDIAEAAPVDGFDVSLKGTNTYEDIKGADVVIVTAG
LPRKPGMSRDDLIAVNSKIMTTVAEGIKQYAPNAFVIVISNPLDAMVTLCQRITGFPHSRVVGQAGVLDSARFAAFIAWE
LGVSVKDVTAVTLGGHGDDMVPLVRYTSVCGVPVMELLEQKYGAAKAAEVMAAMVKRTRGAGGEVVALLKTGSAFYSPAS
SAIAMAESFLKDQKRVLPTCAFLKGEFGVDGLYVGVPVVIGAGGAERVLQLKLNAEEQAMMDKSVKAVKDLVATLK
>Mature_315_residues
ARSKIALIGGGQIGGVLAQLAALRELGDVVLFDIVEGLPQGKTLDIAEAAPVDGFDVSLKGTNTYEDIKGADVVIVTAGL
PRKPGMSRDDLIAVNSKIMTTVAEGIKQYAPNAFVIVISNPLDAMVTLCQRITGFPHSRVVGQAGVLDSARFAAFIAWEL
GVSVKDVTAVTLGGHGDDMVPLVRYTSVCGVPVMELLEQKYGAAKAAEVMAAMVKRTRGAGGEVVALLKTGSAFYSPASS
AIAMAESFLKDQKRVLPTCAFLKGEFGVDGLYVGVPVVIGAGGAERVLQLKLNAEEQAMMDKSVKAVKDLVATLK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=318, Percent_Identity=30.5031446540881, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI221136809, Length=318, Percent_Identity=30.5031446540881, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI15082234, Length=316, Percent_Identity=28.4810126582279, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI9257228, Length=318, Percent_Identity=30.188679245283, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI4504973, Length=318, Percent_Identity=30.188679245283, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI5031857, Length=304, Percent_Identity=29.6052631578947, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI260099723, Length=304, Percent_Identity=29.6052631578947, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI291575128, Length=318, Percent_Identity=28.6163522012579, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI4557032, Length=318, Percent_Identity=28.6163522012579, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI260099727, Length=216, Percent_Identity=29.6296296296296, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI260099725, Length=208, Percent_Identity=30.2884615384615, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI21735621, Length=331, Percent_Identity=27.7945619335347, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI207028494, Length=188, Percent_Identity=31.3829787234043, Blast_Score=98, Evalue=9e-21,
Organism=Escherichia coli, GI1789632, Length=323, Percent_Identity=27.8637770897833, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535107, Length=282, Percent_Identity=24.468085106383, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17554310, Length=259, Percent_Identity=30.5019305019305, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=303, Percent_Identity=27.0627062706271, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=324, Percent_Identity=26.2345679012346, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17136226, Length=320, Percent_Identity=26.5625, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24647881, Length=253, Percent_Identity=31.2252964426877, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550422, Length=302, Percent_Identity=28.476821192053, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24663599, Length=298, Percent_Identity=28.5234899328859, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24663595, Length=254, Percent_Identity=27.9527559055118, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32993; Mature: 32862

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSKIALIGGGQIGGVLAQLAALRELGDVVLFDIVEGLPQGKTLDIAEAAPVDGFDVSL
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEE
KGTNTYEDIKGADVVIVTAGLPRKPGMSRDDLIAVNSKIMTTVAEGIKQYAPNAFVIVIS
ECCCCHHHCCCCCEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEEC
NPLDAMVTLCQRITGFPHSRVVGQAGVLDSARFAAFIAWELGVSVKDVTAVTLGGHGDDM
CCHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEHEEEEEECCCCCCC
VPLVRYTSVCGVPVMELLEQKYGAAKAAEVMAAMVKRTRGAGGEVVALLKTGSAFYSPAS
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHCCCHH
SAIAMAESFLKDQKRVLPTCAFLKGEFGVDGLYVGVPVVIGAGGAERVLQLKLNAEEQAM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHEEEEEEECCHHHHH
MDKSVKAVKDLVATLK
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARSKIALIGGGQIGGVLAQLAALRELGDVVLFDIVEGLPQGKTLDIAEAAPVDGFDVSL
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEE
KGTNTYEDIKGADVVIVTAGLPRKPGMSRDDLIAVNSKIMTTVAEGIKQYAPNAFVIVIS
ECCCCHHHCCCCCEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEEC
NPLDAMVTLCQRITGFPHSRVVGQAGVLDSARFAAFIAWELGVSVKDVTAVTLGGHGDDM
CCHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEHEEEEEECCCCCCC
VPLVRYTSVCGVPVMELLEQKYGAAKAAEVMAAMVKRTRGAGGEVVALLKTGSAFYSPAS
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHCCCHH
SAIAMAESFLKDQKRVLPTCAFLKGEFGVDGLYVGVPVVIGAGGAERVLQLKLNAEEQAM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHEEEEEEECCHHHHH
MDKSVKAVKDLVATLK
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA