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The map label for this gene is polA [H]

Identifier: 220916831

GI number: 220916831

Start: 1927554

End: 1928513

Strand: Direct

Name: polA [H]

Synonym: A2cp1_1727

Alternate gene names: 220916831

Gene position: 1927554-1928513 (Clockwise)

Preceding gene: 220916826

Following gene: 220916832

Centisome position: 38.33

GC content: 78.44

Gene sequence:

>960_bases
ATGAGGCTCCACCTGGTGGACGGCACCTACGAGCTGTTCCGCGCGCACTTCTCGGGGCGGCCGGGCCACCGGGCGCCCGA
CGGCCGCGAGGTGAAGGCGACGCTCGGGATGGCCGCCTCGCTCCTGGCGCTGCTGCACGAGGCCGGTGAGGCGGTGACGC
ACGTGGCGGTGGCGTTCGACCGGCCCATCCGCTCGTTCCGCAACGACCTGTGGGCCGGCTACAAGAGCGAGGAGGGCGTC
GAGCCCGAGCTGCTCGCGCAGCTCGAGGCCGCGGAGGAGGCCGCCCGGGCGCTCGGGCTGGTGGTGTGGTCGATGGACGC
GCTGGAGGCCGACGACGCGCTCGCCACCGCGGCGGCCCGCTTCCGCGACGAGGTGGAGCAGGTGCGGATCCTCACCCCGG
ACAAGGACCTCGGTCAGTGCCTCCGCGGGCGGCGGGTGGTCCAGGTGGATCGCATGCGCGGGCGCGAGACCGACGAGGCG
GCGCTGCTCGCGCGCCGCGGCATCCAGCCGGAGAGCGTCCCCGACTGGCTGGCGCTGGTGGGGGACACCGCGGACGGGAT
CCCGGGGCTGCCCGGCTTCGGCGAGCGCACCGCGTCGGCGCTGCTCGCCGTGTACGGGCACCTGGAGGACGTCCCGGCCG
ACCCGGCGCGCTGGCCGGCGGGCGTGCGCGGCGCGGCGAAGCTGGCCCGGGCGCTGGAGGAGGGGCGCGAGGCGGCGCTG
CTGTACCGGCGGCTCGCGACGCTTGTCACCGACGCGCCGCTGGCCGAGGGGCTCGAGGCGCTGCGCTGGCCCGGGCTCTC
GCGCGAGCGGCTCGGCGCCTGGGCGGCGTCGCTCGGGGCCGAGCGGCTGGTGCAGGGGCTGGAGGCGCGACCGGCGCGGT
GGGCGGGCCCCGGTGGCGCGCCGCCGCCGGCGGCGGGGCAGGGCGAGGCCCGCGACGTGGCGGGCCGGGAGGGGACATGA

Upstream 100 bases:

>100_bases
GGGGGCGCACGCGCGGGGTGCGGGCCACGCAGGTACCAGCCAGGGCGCGGCGGCGCGCGCACGCCCCGGGCGCGGGTTCG
CGGAGCGTATACTTCGCGCC

Downstream 100 bases:

>100_bases
CGCTCCGCCGCGACTTCATCCAGCGCATGCTGGAGCAGCTCGGCTGGGCGCTCGCCGGGGTGCTGAAGCTCCGCCGGGCC
GGCGCGCACGAGCAGGCGGT

Product: 5'-3' exonuclease

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRLHLVDGTYELFRAHFSGRPGHRAPDGREVKATLGMAASLLALLHEAGEAVTHVAVAFDRPIRSFRNDLWAGYKSEEGV
EPELLAQLEAAEEAARALGLVVWSMDALEADDALATAAARFRDEVEQVRILTPDKDLGQCLRGRRVVQVDRMRGRETDEA
ALLARRGIQPESVPDWLALVGDTADGIPGLPGFGERTASALLAVYGHLEDVPADPARWPAGVRGAAKLARALEEGREAAL
LYRRLATLVTDAPLAEGLEALRWPGLSRERLGAWAASLGAERLVQGLEARPARWAGPGGAPPPAAGQGEARDVAGREGT

Sequences:

>Translated_319_residues
MRLHLVDGTYELFRAHFSGRPGHRAPDGREVKATLGMAASLLALLHEAGEAVTHVAVAFDRPIRSFRNDLWAGYKSEEGV
EPELLAQLEAAEEAARALGLVVWSMDALEADDALATAAARFRDEVEQVRILTPDKDLGQCLRGRRVVQVDRMRGRETDEA
ALLARRGIQPESVPDWLALVGDTADGIPGLPGFGERTASALLAVYGHLEDVPADPARWPAGVRGAAKLARALEEGREAAL
LYRRLATLVTDAPLAEGLEALRWPGLSRERLGAWAASLGAERLVQGLEARPARWAGPGGAPPPAAGQGEARDVAGREGT
>Mature_319_residues
MRLHLVDGTYELFRAHFSGRPGHRAPDGREVKATLGMAASLLALLHEAGEAVTHVAVAFDRPIRSFRNDLWAGYKSEEGV
EPELLAQLEAAEEAARALGLVVWSMDALEADDALATAAARFRDEVEQVRILTPDKDLGQCLRGRRVVQVDRMRGRETDEA
ALLARRGIQPESVPDWLALVGDTADGIPGLPGFGERTASALLAVYGHLEDVPADPARWPAGVRGAAKLARALEEGREAAL
LYRRLATLVTDAPLAEGLEALRWPGLSRERLGAWAASLGAERLVQGLEARPARWAGPGGAPPPAAGQGEARDVAGREGT

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=307, Percent_Identity=33.5504885993485, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI226510970, Length=213, Percent_Identity=30.0469483568075, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34136; Mature: 34136

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLHLVDGTYELFRAHFSGRPGHRAPDGREVKATLGMAASLLALLHEAGEAVTHVAVAFD
CEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPIRSFRNDLWAGYKSEEGVEPELLAQLEAAEEAARALGLVVWSMDALEADDALATAAAR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRDEVEQVRILTPDKDLGQCLRGRRVVQVDRMRGRETDEAALLARRGIQPESVPDWLALV
HHHHHHHEEECCCCHHHHHHHHCCEEEEHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHH
GDTADGIPGLPGFGERTASALLAVYGHLEDVPADPARWPAGVRGAAKLARALEEGREAAL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LYRRLATLVTDAPLAEGLEALRWPGLSRERLGAWAASLGAERLVQGLEARPARWAGPGGA
HHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PPPAAGQGEARDVAGREGT
CCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MRLHLVDGTYELFRAHFSGRPGHRAPDGREVKATLGMAASLLALLHEAGEAVTHVAVAFD
CEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPIRSFRNDLWAGYKSEEGVEPELLAQLEAAEEAARALGLVVWSMDALEADDALATAAAR
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRDEVEQVRILTPDKDLGQCLRGRRVVQVDRMRGRETDEAALLARRGIQPESVPDWLALV
HHHHHHHEEECCCCHHHHHHHHCCEEEEHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHH
GDTADGIPGLPGFGERTASALLAVYGHLEDVPADPARWPAGVRGAAKLARALEEGREAAL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LYRRLATLVTDAPLAEGLEALRWPGLSRERLGAWAASLGAERLVQGLEARPARWAGPGGA
HHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PPPAAGQGEARDVAGREGT
CCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6276402; 8346018; 9278503; 6183253; 6302278; 7035456; 3883192; 1989886; 8469987; 8260491; 9514742; 9888810; 10588690; 8442659 [H]