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The map label for this gene is birA [H]

Identifier: 220916677

GI number: 220916677

Start: 1758988

End: 1759980

Strand: Direct

Name: birA [H]

Synonym: A2cp1_1571

Alternate gene names: 220916677

Gene position: 1758988-1759980 (Clockwise)

Preceding gene: 220916676

Following gene: 220916678

Centisome position: 34.97

GC content: 76.03

Gene sequence:

>993_bases
ATGGCGACCGAGCCGTCCGACTCCGCCGAGCTGGTGCTCGCGTTCCTGGCCGAGGCCGGGGACGAGGTCGTGTCGGGCGA
GGCCATCTCCGACAAGCTGGGCCTGACCCGCGCCGCGGTGTGGAAGCACGTGAACGCGCTCCGTGCCCGCGGCTACCGCA
TCGACGCGGTGCCGGCGCGCGGCTACCGCCTGGCCGAGGTGCCGGACCGGCTCACCGCGCTGGAGCTGCGACCGCTGCTC
AACACCCACGACCTGGGGCAGGTGCTCCACGCGCACGAGGAGCTCGCCTCGACGAACGACCGCGCCCGGGAGCTGGCCGA
GGAGGGCGCCGCCCACGGCGAGGTGGTGATCGCCGAGCGTCAGACCGCCGGTCGGGGACGGCGCGGCCGCGCCTGGATCT
CGCCCGCGCGCAAGAACCTCTACTTCTCCGCGATCCTGCGGCCCGACCTGCCCACGGCGCGCGCGCCGGAGGTCACGCTG
GTCGCCTCGCTGGCGGTCTGCGACGTCCTCCGGCAGGCCGGGGTGGACGCGGGCATCAAGTGGCCGAACGACGTGCTCGC
GGGCGGGCGCAAGATCGCCGGGATCCTCACCGAGCTGGCGGCCGAGCCCGACCGCGTCCACTGGATCGTCGTGGGCGTCG
GCGTGAACGTGAACGCCCGGGCCGAGGACTTCCCGGACGACCTGCGCGGCGAGGCCACCAGCGTGCTGCTGGAGCGCGGC
GAGCCCGCGCCGCGCGCGCTGTTCGCCGCCGCCTGCCTGACCGCGCTGGAGGGCTGGCTGGACGTGCACGAGGAGCAGGG
CTTCGGGCCCATCCGCGAGGCGTGGCGCGAGCGGTCGGTGACGCTGGGGCGCGAGGTGCGGGTGCGGGTGGACGACGGCG
AGCTGGCGGGCACGGCCGAGGACATCGACGACGCCGGGGCCCTGCTGGTCCGGACGGCGAGCGGGCTGGAGCGGGTGCTC
GCCGGCGACGTCCGCCTGCTGCGGCCCCGCTGA

Upstream 100 bases:

>100_bases
CGCGCGGGCCGTGGACCTCGCGTTCGACCTCGCCTGAGCCGCGCCCTGCCCGGGCGCGCCATTTGACAGGCCGCCGCCGG
CCCCGGAAGATCCCGGGACC

Downstream 100 bases:

>100_bases
CCCGCGCGCCGGCGCGCGCCCGCGGCGGCCGTGGAATTGACCCCGGACAGGCCCGGAAGCTAGGCTCCGGCCCGTGCTCC
TCGCCATCGACGTCGGCAAC

Product: biotin/acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MATEPSDSAELVLAFLAEAGDEVVSGEAISDKLGLTRAAVWKHVNALRARGYRIDAVPARGYRLAEVPDRLTALELRPLL
NTHDLGQVLHAHEELASTNDRARELAEEGAAHGEVVIAERQTAGRGRRGRAWISPARKNLYFSAILRPDLPTARAPEVTL
VASLAVCDVLRQAGVDAGIKWPNDVLAGGRKIAGILTELAAEPDRVHWIVVGVGVNVNARAEDFPDDLRGEATSVLLERG
EPAPRALFAAACLTALEGWLDVHEEQGFGPIREAWRERSVTLGREVRVRVDDGELAGTAEDIDDAGALLVRTASGLERVL
AGDVRLLRPR

Sequences:

>Translated_330_residues
MATEPSDSAELVLAFLAEAGDEVVSGEAISDKLGLTRAAVWKHVNALRARGYRIDAVPARGYRLAEVPDRLTALELRPLL
NTHDLGQVLHAHEELASTNDRARELAEEGAAHGEVVIAERQTAGRGRRGRAWISPARKNLYFSAILRPDLPTARAPEVTL
VASLAVCDVLRQAGVDAGIKWPNDVLAGGRKIAGILTELAAEPDRVHWIVVGVGVNVNARAEDFPDDLRGEATSVLLERG
EPAPRALFAAACLTALEGWLDVHEEQGFGPIREAWRERSVTLGREVRVRVDDGELAGTAEDIDDAGALLVRTASGLERVL
AGDVRLLRPR
>Mature_329_residues
ATEPSDSAELVLAFLAEAGDEVVSGEAISDKLGLTRAAVWKHVNALRARGYRIDAVPARGYRLAEVPDRLTALELRPLLN
THDLGQVLHAHEELASTNDRARELAEEGAAHGEVVIAERQTAGRGRRGRAWISPARKNLYFSAILRPDLPTARAPEVTLV
ASLAVCDVLRQAGVDAGIKWPNDVLAGGRKIAGILTELAAEPDRVHWIVVGVGVNVNARAEDFPDDLRGEATSVLLERGE
PAPRALFAAACLTALEGWLDVHEEQGFGPIREAWRERSVTLGREVRVRVDDGELAGTAEDIDDAGALLVRTASGLERVLA
GDVRLLRPR

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=252, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790408, Length=320, Percent_Identity=34.0625, Blast_Score=154, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35593; Mature: 35461

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEPSDSAELVLAFLAEAGDEVVSGEAISDKLGLTRAAVWKHVNALRARGYRIDAVPAR
CCCCCCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCC
GYRLAEVPDRLTALELRPLLNTHDLGQVLHAHEELASTNDRARELAEEGAAHGEVVIAER
CEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEC
QTAGRGRRGRAWISPARKNLYFSAILRPDLPTARAPEVTLVASLAVCDVLRQAGVDAGIK
CCCCCCCCCCCCCCHHHHCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
WPNDVLAGGRKIAGILTELAAEPDRVHWIVVGVGVNVNARAEDFPDDLRGEATSVLLERG
CCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHHCCHHHHHHHHCC
EPAPRALFAAACLTALEGWLDVHEEQGFGPIREAWRERSVTLGREVRVRVDDGELAGTAE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCHH
DIDDAGALLVRTASGLERVLAGDVRLLRPR
HHCCCCCEEEHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
ATEPSDSAELVLAFLAEAGDEVVSGEAISDKLGLTRAAVWKHVNALRARGYRIDAVPAR
CCCCCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCC
GYRLAEVPDRLTALELRPLLNTHDLGQVLHAHEELASTNDRARELAEEGAAHGEVVIAER
CEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEC
QTAGRGRRGRAWISPARKNLYFSAILRPDLPTARAPEVTLVASLAVCDVLRQAGVDAGIK
CCCCCCCCCCCCCCHHHHCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
WPNDVLAGGRKIAGILTELAAEPDRVHWIVVGVGVNVNARAEDFPDDLRGEATSVLLERG
CCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHHCCHHHHHHHHCC
EPAPRALFAAACLTALEGWLDVHEEQGFGPIREAWRERSVTLGREVRVRVDDGELAGTAE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCHH
DIDDAGALLVRTASGLERVLAGDVRLLRPR
HHCCCCCEEEHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]