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The map label for this gene is epsE [H]

Identifier: 220915837

GI number: 220915837

Start: 808281

End: 810080

Strand: Reverse

Name: epsE [H]

Synonym: A2cp1_0720

Alternate gene names: 220915837

Gene position: 810080-808281 (Counterclockwise)

Preceding gene: 220915838

Following gene: 220915836

Centisome position: 16.11

GC content: 70.89

Gene sequence:

>1800_bases
ATGCCCAACGACGCACAGATCGACAACGCCCGGGCCGCCGTCCAGGGCGCCATCCCCGGGCTCGCCGCCGCGGCCGGGCT
CGCCGGCCGTCCCATCGGCGAGATCCTCCGGGAGACGGCCGGGCTGGACCCGGCGCGCCTCGAGGAGGCGCTCGCGCTGC
AGCAGGGCGAGCAGGCCGGCCAGCGCCTCGGCGAGATCCTCGTCCGCCTGAGGGCGGTCACCGAGGAGGACGTGCTGCGC
GCGCTCGCGGCCCAGCTCGACCTGCCGTTCGTCGAGCGCATCGACCCGGAGTCGGTGCCGGGCGACCTCGCGGCCAAGGT
GCCGATCCACTTCGCCAAGCAGTCGAAGGTGCTGCCGCTCGGCACCGCCGGTGACGCGGTCCGGGTGGCGGTGGGCGACC
CGCTCGACACCGCCGCGCAGGACTCGGTGTCCATGCTGCTCGGCGCCGCGGTGGCGCCGGAGGTGGCGCCGCCGCAGGTG
GTGCTCGACGCCATCAACGCGGTGTACGACCGCGCCGCCGACGAGCACGACAAGCTCATGGAGGACCTCGAGACCGAGGA
CCTCGAGAGCGTCGCGCACGAGCTGCAGGAGCCGACCGACCTCCTCGAGGCGGACGACGAGGCGCCCATCATCCGCCTGG
TCAACTCGCTCCTGTTCCGCGCGGTGAAGGAGCGCGCCAGCGACATCCACATCCTCCCGGAGGAGAAGGACATCTCGGTG
CGCTACCGGATCGACGGCGTGCTCCGCGAGGTGATCCGCCCTCCCAAGCGCTTCCAGGCGTCCATCGGCAGCCGCATCAA
GATCATGGGCGGGCTGAACATCGCCGAGAAGCGGCTCCCGCAGGACGGCCGCATCCGCATCAAGATCGCCGGCAAGGACG
TGGACATCCGCCTGTCCACCGTCCCCACCGCGCACGGCGAGCGCATCGTGATGCGCCTCCTCGACAAGTCCAACGTGGTG
CTGGACCTGGCCGAGCTCGGCTTCGAGGACTGGCAGCTCAAGGTGATGGACACGCTCATCACCCGCTCGCACGGCATCGT
GCTGGTCACCGGCCCGACCGGCTCCGGCAAGACCACGACGCTCTACGCGGCGCTCGCGAAGATCAACTCGCCCGACCTGA
ACATCCTCACCATCGAGGATCCGGTCGAGATCCCGCTGAAGGGCGTGGGGCAGGTGCAGATCAACCCGAAGATCGACCTG
ACCTTCGCGAGCGGGCTGCGCTCGTTCCTCCGGCAGGACCCGGACGTCATCATGGTCGGCGAGATCCGCGACCTGGAGAC
CGCCGAGATCGCGATCCAGGCCTCGCTCACCGGCCACCTGGTGCTCTCCACCGTGCACACCAACGACGCCGCCGGGGCCA
TCACGCGCCTGGTGGACATGGGCGTGCAGCCGTTCCTGGTGGCGTCCTCGCTGGTGGGCGTGCTGGCGCAGCGCCTGGTG
CGCGTGCTCTGCCCGACGTGCAAGAAGCCCTACGTCCCCACCGCCGAGGAGCGCGACCTCGCCGGGCTCACGCCCGAGAT
CCTGCGCCAGGCCGGCGACCCGACCCACCTCTACAAGGCGGTGGGGTGCCCGGCCTGCAACCACACCGGCTACCAGGGCC
GGACCGGCATCTACGAGCTGATGATGGTGGACGACGACGTCCGCCCGCTCATCCTCAAGTCGGTGGACGCCACCACCATC
AAGCGCGCCGCCGTCGAGCGCGGGATGGTGACGCTCATGGAGCACGGCGCGTACAAGGTCGCGCGCGGCATCACCACCGC
GGCCGAGGTGCTGTCGGTCACCGCGGAGGACATCCGGTGA

Upstream 100 bases:

>100_bases
CTCGCCGCAGCAGCCGCCGCCCGCCGCGGAGCCGGCCGCGCCCGGAGGGACCCCACCGCAGGAATGAGCGGGGGCGGACC
GTCCGTTTCACGGGATCCTC

Downstream 100 bases:

>100_bases
GCGGCGCGCCGCGCGCCCACGGAGCCCCCTGATGCCGGTCTTCGAGTACAAGGCGTACGACGCCCAGGGCAAGGCGATCC
AGGGCCTGCGCGAGGCGGAC

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MPNDAQIDNARAAVQGAIPGLAAAAGLAGRPIGEILRETAGLDPARLEEALALQQGEQAGQRLGEILVRLRAVTEEDVLR
ALAAQLDLPFVERIDPESVPGDLAAKVPIHFAKQSKVLPLGTAGDAVRVAVGDPLDTAAQDSVSMLLGAAVAPEVAPPQV
VLDAINAVYDRAADEHDKLMEDLETEDLESVAHELQEPTDLLEADDEAPIIRLVNSLLFRAVKERASDIHILPEEKDISV
RYRIDGVLREVIRPPKRFQASIGSRIKIMGGLNIAEKRLPQDGRIRIKIAGKDVDIRLSTVPTAHGERIVMRLLDKSNVV
LDLAELGFEDWQLKVMDTLITRSHGIVLVTGPTGSGKTTTLYAALAKINSPDLNILTIEDPVEIPLKGVGQVQINPKIDL
TFASGLRSFLRQDPDVIMVGEIRDLETAEIAIQASLTGHLVLSTVHTNDAAGAITRLVDMGVQPFLVASSLVGVLAQRLV
RVLCPTCKKPYVPTAEERDLAGLTPEILRQAGDPTHLYKAVGCPACNHTGYQGRTGIYELMMVDDDVRPLILKSVDATTI
KRAAVERGMVTLMEHGAYKVARGITTAAEVLSVTAEDIR

Sequences:

>Translated_599_residues
MPNDAQIDNARAAVQGAIPGLAAAAGLAGRPIGEILRETAGLDPARLEEALALQQGEQAGQRLGEILVRLRAVTEEDVLR
ALAAQLDLPFVERIDPESVPGDLAAKVPIHFAKQSKVLPLGTAGDAVRVAVGDPLDTAAQDSVSMLLGAAVAPEVAPPQV
VLDAINAVYDRAADEHDKLMEDLETEDLESVAHELQEPTDLLEADDEAPIIRLVNSLLFRAVKERASDIHILPEEKDISV
RYRIDGVLREVIRPPKRFQASIGSRIKIMGGLNIAEKRLPQDGRIRIKIAGKDVDIRLSTVPTAHGERIVMRLLDKSNVV
LDLAELGFEDWQLKVMDTLITRSHGIVLVTGPTGSGKTTTLYAALAKINSPDLNILTIEDPVEIPLKGVGQVQINPKIDL
TFASGLRSFLRQDPDVIMVGEIRDLETAEIAIQASLTGHLVLSTVHTNDAAGAITRLVDMGVQPFLVASSLVGVLAQRLV
RVLCPTCKKPYVPTAEERDLAGLTPEILRQAGDPTHLYKAVGCPACNHTGYQGRTGIYELMMVDDDVRPLILKSVDATTI
KRAAVERGMVTLMEHGAYKVARGITTAAEVLSVTAEDIR
>Mature_598_residues
PNDAQIDNARAAVQGAIPGLAAAAGLAGRPIGEILRETAGLDPARLEEALALQQGEQAGQRLGEILVRLRAVTEEDVLRA
LAAQLDLPFVERIDPESVPGDLAAKVPIHFAKQSKVLPLGTAGDAVRVAVGDPLDTAAQDSVSMLLGAAVAPEVAPPQVV
LDAINAVYDRAADEHDKLMEDLETEDLESVAHELQEPTDLLEADDEAPIIRLVNSLLFRAVKERASDIHILPEEKDISVR
YRIDGVLREVIRPPKRFQASIGSRIKIMGGLNIAEKRLPQDGRIRIKIAGKDVDIRLSTVPTAHGERIVMRLLDKSNVVL
DLAELGFEDWQLKVMDTLITRSHGIVLVTGPTGSGKTTTLYAALAKINSPDLNILTIEDPVEIPLKGVGQVQINPKIDLT
FASGLRSFLRQDPDVIMVGEIRDLETAEIAIQASLTGHLVLSTVHTNDAAGAITRLVDMGVQPFLVASSLVGVLAQRLVR
VLCPTCKKPYVPTAEERDLAGLTPEILRQAGDPTHLYKAVGCPACNHTGYQGRTGIYELMMVDDDVRPLILKSVDATTIK
RAAVERGMVTLMEHGAYKVARGITTAAEVLSVTAEDIR

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=432, Percent_Identity=49.3055555555556, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1786296, Length=449, Percent_Identity=40.3118040089087, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI87082188, Length=213, Percent_Identity=40.3755868544601, Blast_Score=120, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 64492; Mature: 64361

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNDAQIDNARAAVQGAIPGLAAAAGLAGRPIGEILRETAGLDPARLEEALALQQGEQAG
CCCCCCCCCHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QRLGEILVRLRAVTEEDVLRALAAQLDLPFVERIDPESVPGDLAAKVPIHFAKQSKVLPL
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEECCCEEECCCCCEEEE
GTAGDAVRVAVGDPLDTAAQDSVSMLLGAAVAPEVAPPQVVLDAINAVYDRAADEHDKLM
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
EDLETEDLESVAHELQEPTDLLEADDEAPIIRLVNSLLFRAVKERASDIHILPEEKDISV
HHHCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEE
RYRIDGVLREVIRPPKRFQASIGSRIKIMGGLNIAEKRLPQDGRIRIKIAGKDVDIRLST
EEEHHHHHHHHHCCHHHHHHHCCCEEEEEECCCHHHHHCCCCCCEEEEEECCCEEEEEEE
VPTAHGERIVMRLLDKSNVVLDLAELGFEDWQLKVMDTLITRSHGIVLVTGPTGSGKTTT
CCCCCHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHH
LYAALAKINSPDLNILTIEDPVEIPLKGVGQVQINPKIDLTFASGLRSFLRQDPDVIMVG
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECCEEEEEHHHHHHHHHHCCCCEEEEC
EIRDLETAEIAIQASLTGHLVLSTVHTNDAAGAITRLVDMGVQPFLVASSLVGVLAQRLV
CCCCCCCEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RVLCPTCKKPYVPTAEERDLAGLTPEILRQAGDPTHLYKAVGCPACNHTGYQGRTGIYEL
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEE
MMVDDDVRPLILKSVDATTIKRAAVERGMVTLMEHGAYKVARGITTAAEVLSVTAEDIR
EEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNDAQIDNARAAVQGAIPGLAAAAGLAGRPIGEILRETAGLDPARLEEALALQQGEQAG
CCCCCCCCHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QRLGEILVRLRAVTEEDVLRALAAQLDLPFVERIDPESVPGDLAAKVPIHFAKQSKVLPL
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEECCCEEECCCCCEEEE
GTAGDAVRVAVGDPLDTAAQDSVSMLLGAAVAPEVAPPQVVLDAINAVYDRAADEHDKLM
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
EDLETEDLESVAHELQEPTDLLEADDEAPIIRLVNSLLFRAVKERASDIHILPEEKDISV
HHHCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEE
RYRIDGVLREVIRPPKRFQASIGSRIKIMGGLNIAEKRLPQDGRIRIKIAGKDVDIRLST
EEEHHHHHHHHHCCHHHHHHHCCCEEEEEECCCHHHHHCCCCCCEEEEEECCCEEEEEEE
VPTAHGERIVMRLLDKSNVVLDLAELGFEDWQLKVMDTLITRSHGIVLVTGPTGSGKTTT
CCCCCHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHH
LYAALAKINSPDLNILTIEDPVEIPLKGVGQVQINPKIDLTFASGLRSFLRQDPDVIMVG
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECCEEEEEHHHHHHHHHHCCCCEEEEC
EIRDLETAEIAIQASLTGHLVLSTVHTNDAAGAITRLVDMGVQPFLVASSLVGVLAQRLV
CCCCCCCEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RVLCPTCKKPYVPTAEERDLAGLTPEILRQAGDPTHLYKAVGCPACNHTGYQGRTGIYEL
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEE
MMVDDDVRPLILKSVDATTIKRAAVERGMVTLMEHGAYKVARGITTAAEVLSVTAEDIR
EEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]