Click here to switch to the map view.

The map label for this gene is iscS

Identifier: 220915737

GI number: 220915737

Start: 696058

End: 697272

Strand: Direct

Name: iscS

Synonym: A2cp1_0618

Alternate gene names: 220915737

Gene position: 696058-697272 (Clockwise)

Preceding gene: 220915735

Following gene: 220915738

Centisome position: 13.84

GC content: 69.05

Gene sequence:

>1215_bases
ATGAAGATTCCCATCTACATGGATTACCACGCGACGACGCCCGTCGATCCGCGCGTGCTGGAGGCCATGCTCCCGTACTT
CACCACGGAGTACGGGAACGCCGCGTCGAAGAGCCACGCCTTCGGCTGGAAGGCCGAGGAGGCGGTGGAGGCAGCCCGCG
AGGAGGTCGCGAAGCTGATCGGCGCCTCGGCCAAGGAGATCGTCTGGACGAGCGGCGCGACCGAGTCCGACAACCTCGCG
GTCAAGGGCGCGGCGCACTTCTACCAGGCGAAGGGCAAGCACCTCGTCACCTGCAAGACCGAGCACAAGGCGGTGCTCGA
CTCGATGCACGCCCTCGAGCGGCAGGGCTTCGAGGTCACCTTCCTCGAGCCCGAGCGCGACGGCCGGCTCGATCCCGCCA
AGGTGCAGGCGGCGCTGCGCCCCGACACGATCCTCGTCTCGGTGATGCACGCGAACAACGAGACCGGCGTGGTCCACCCC
ATCGCCGAGATCGGCCGCATCGCCCGCGGGGCGGGCGTGGTGTTCCACTGCGACGCGGTGCAGTCGATCGGCAAGATCCC
GTTCGACGTCGAGGCGATGAACGTGGATCTCGCCTCGATCTCCGCGCACAAGATGTACGGCCCGAAGGGGATGGGCGCGC
TCTACGTCCGCCGCAAGCCGCGCGTGCGCCTCGTCGCCGAGATGGACGGCGGCGGCCACGAGCGCGGCTTCCGCTCCGGC
ACGCTGAACGTCCCCGGCATCGTGGGCATGGGCAAGGCGGCCGAGCTGGCGCGGCTGGAGCGCGACGCCGAGGCGGTCCG
CGTCACCGCGCTCCGCGAGCGGCTGCGCAAGGGCCTGGAGAAGGAGCTCGATCTCCTGACCGTGAACGGGAGCCTGGAGC
ACCGCGTCCCCGGCAACCTCAACGTGAGCTTCGCCTACGTCGAGGGCGAGGCGCTCATGATGGCGGTGAAGGACGTGGCC
GTCTCCTCCGGCTCCGCCTGCACCAGCGCGTCGCTCGAGCCGTCGTACGTGCTCCGGGCGATGGGCGTGTCCGAGGACCT
GGCCCACTCGTCGATCCGCTTCGGCCTGGGCCGGTTCAGCACGGAGGAGGAGGTGGACTACACGATCCGCCTCTTCGGCG
AGAAGGTCCGCAAGCTGCGCGAGATGTCGCCGCTCTACGAGATGGTGAAGGACGGCGTCGACCTGAACCAGATCGAGTGG
GCGAACCCACACTAG

Upstream 100 bases:

>100_bases
GGGGGGCCGCTTGACCCGATGACGTGAATCTTTATACTCGACCCCGGCCGTCCACGTTGGTCACGGCCGAGGCCCCTGCA
TCTTAGAGGTTCCGGGCGCG

Downstream 100 bases:

>100_bases
GACCGGGACTGCTCGAGCCGGTCCACGTGGACGGGAACGAGGGAAGGAACGACCGATGGCGTACTCCGAGAAGCTCATCG
ACCACTACGAGCACCCCCGC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKIPIYMDYHATTPVDPRVLEAMLPYFTTEYGNAASKSHAFGWKAEEAVEAAREEVAKLIGASAKEIVWTSGATESDNLA
VKGAAHFYQAKGKHLVTCKTEHKAVLDSMHALERQGFEVTFLEPERDGRLDPAKVQAALRPDTILVSVMHANNETGVVHP
IAEIGRIARGAGVVFHCDAVQSIGKIPFDVEAMNVDLASISAHKMYGPKGMGALYVRRKPRVRLVAEMDGGGHERGFRSG
TLNVPGIVGMGKAAELARLERDAEAVRVTALRERLRKGLEKELDLLTVNGSLEHRVPGNLNVSFAYVEGEALMMAVKDVA
VSSGSACTSASLEPSYVLRAMGVSEDLAHSSIRFGLGRFSTEEEVDYTIRLFGEKVRKLREMSPLYEMVKDGVDLNQIEW
ANPH

Sequences:

>Translated_404_residues
MKIPIYMDYHATTPVDPRVLEAMLPYFTTEYGNAASKSHAFGWKAEEAVEAAREEVAKLIGASAKEIVWTSGATESDNLA
VKGAAHFYQAKGKHLVTCKTEHKAVLDSMHALERQGFEVTFLEPERDGRLDPAKVQAALRPDTILVSVMHANNETGVVHP
IAEIGRIARGAGVVFHCDAVQSIGKIPFDVEAMNVDLASISAHKMYGPKGMGALYVRRKPRVRLVAEMDGGGHERGFRSG
TLNVPGIVGMGKAAELARLERDAEAVRVTALRERLRKGLEKELDLLTVNGSLEHRVPGNLNVSFAYVEGEALMMAVKDVA
VSSGSACTSASLEPSYVLRAMGVSEDLAHSSIRFGLGRFSTEEEVDYTIRLFGEKVRKLREMSPLYEMVKDGVDLNQIEW
ANPH
>Mature_404_residues
MKIPIYMDYHATTPVDPRVLEAMLPYFTTEYGNAASKSHAFGWKAEEAVEAAREEVAKLIGASAKEIVWTSGATESDNLA
VKGAAHFYQAKGKHLVTCKTEHKAVLDSMHALERQGFEVTFLEPERDGRLDPAKVQAALRPDTILVSVMHANNETGVVHP
IAEIGRIARGAGVVFHCDAVQSIGKIPFDVEAMNVDLASISAHKMYGPKGMGALYVRRKPRVRLVAEMDGGGHERGFRSG
TLNVPGIVGMGKAAELARLERDAEAVRVTALRERLRKGLEKELDLLTVNGSLEHRVPGNLNVSFAYVEGEALMMAVKDVA
VSSGSACTSASLEPSYVLRAMGVSEDLAHSSIRFGLGRFSTEEEVDYTIRLFGEKVRKLREMSPLYEMVKDGVDLNQIEW
ANPH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=399, Percent_Identity=59.3984962406015, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=35.8173076923077, Blast_Score=221, Evalue=1e-57,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=63.1188118811881, Blast_Score=536, Evalue=1e-154,
Organism=Escherichia coli, GI1787970, Length=386, Percent_Identity=27.720207253886, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789175, Length=269, Percent_Identity=29.7397769516729, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25143064, Length=400, Percent_Identity=55.5, Blast_Score=471, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17533177, Length=318, Percent_Identity=30.8176100628931, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=398, Percent_Identity=56.78391959799, Blast_Score=478, Evalue=1e-136,
Organism=Drosophila melanogaster, GI20129463, Length=399, Percent_Identity=58.3959899749373, Blast_Score=479, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_ANAD2 (B8JC53)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002491041.1
- ProteinModelPortal:   B8JC53
- SMR:   B8JC53
- GeneID:   7300319
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0618
- HOGENOM:   HBG635316
- ProtClustDB:   PRK14012
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR02006

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 44171; Mature: 44171

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 327-327

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPIYMDYHATTPVDPRVLEAMLPYFTTEYGNAASKSHAFGWKAEEAVEAAREEVAKLI
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GASAKEIVWTSGATESDNLAVKGAAHFYQAKGKHLVTCKTEHKAVLDSMHALERQGFEVT
CCCCHHEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHCCCEEE
FLEPERDGRLDPAKVQAALRPDTILVSVMHANNETGVVHPIAEIGRIARGAGVVFHCDAV
EECCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCEEECHHHHHHHHCCCCEEEEEHHH
QSIGKIPFDVEAMNVDLASISAHKMYGPKGMGALYVRRKPRVRLVAEMDGGGHERGFRSG
HHHCCCCCEEEEEEEEHHHCCHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCC
TLNVPGIVGMGKAAELARLERDAEAVRVTALRERLRKGLEKELDLLTVNGSLEHRVPGNL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
NVSFAYVEGEALMMAVKDVAVSSGSACTSASLEPSYVLRAMGVSEDLAHSSIRFGLGRFS
EEEEEEECCCEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCC
TEEEVDYTIRLFGEKVRKLREMSPLYEMVKDGVDLNQIEWANPH
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
>Mature Secondary Structure
MKIPIYMDYHATTPVDPRVLEAMLPYFTTEYGNAASKSHAFGWKAEEAVEAAREEVAKLI
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GASAKEIVWTSGATESDNLAVKGAAHFYQAKGKHLVTCKTEHKAVLDSMHALERQGFEVT
CCCCHHEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHCCCEEE
FLEPERDGRLDPAKVQAALRPDTILVSVMHANNETGVVHPIAEIGRIARGAGVVFHCDAV
EECCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCEEECHHHHHHHHCCCCEEEEEHHH
QSIGKIPFDVEAMNVDLASISAHKMYGPKGMGALYVRRKPRVRLVAEMDGGGHERGFRSG
HHHCCCCCEEEEEEEEHHHCCHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCC
TLNVPGIVGMGKAAELARLERDAEAVRVTALRERLRKGLEKELDLLTVNGSLEHRVPGNL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
NVSFAYVEGEALMMAVKDVAVSSGSACTSASLEPSYVLRAMGVSEDLAHSSIRFGLGRFS
EEEEEEECCCEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCC
TEEEVDYTIRLFGEKVRKLREMSPLYEMVKDGVDLNQIEWANPH
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA