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The map label for this gene is norB [H]

Identifier: 220915701

GI number: 220915701

Start: 656342

End: 657829

Strand: Direct

Name: norB [H]

Synonym: A2cp1_0582

Alternate gene names: 220915701

Gene position: 656342-657829 (Clockwise)

Preceding gene: 220915698

Following gene: 220915706

Centisome position: 13.05

GC content: 72.04

Gene sequence:

>1488_bases
ATGGACACGACCGGCACCCGCACGGCGAGGGACGGCGTCGCGTCGTTCCACGGCGGCGATCGCCTCCTGCTCGGGATCAT
CCTGGGCGTCCTGACCTTCTGGCTGTTCGCGCAGACGACGCTCAACATCGCGCCCGACATGCGCCGCGACCTCGCCACCG
GCGAGAGCGTGATGAACATCGCCGTCGCGCTCACCGCGCTGTTCTCCGGCATCTTCATCGTGGTGATGGGCGGCCTCGCA
GATCGCGTCGGGCGCGTGAGGATGGTCCGGCTGGGGTTCCTGGCGAGCATCGCCGGCTCGCTGCTGGTCGGCCTCGCGCC
GGCCGGCGGGCTGGCGGTGGCGTTCCTCCTGGCTGGGCGCGCGCTGCAGGGGCTCTCGGGGGCGTGCATCATGCCGGCGA
GCCTCGCGCTCGTGAAGGCGTACTGGGACGGCGCCGAGCGGCAGCGCGCGATCAGCCTGTGGTCGATCGGCTCCTGGGGC
GGCTCCGGGGTGTGCTCCCTGTTCGGCGGGCTCGTGAGCCAGAACCTGGGCTGGCGCTGGATCTTCTTCGGCTCGGTGCT
GGTCGCCGTCATCGGGCTGCTGCTGCTGCGCGGGACGCCAGAGAGCCGGGCCGAGGGCGGCGCGGCGGACTACCGGTTCG
ACCTCGCCGGCGTGCTCACGTTCATGGTCGCGATGGTGGCCCTGCAGGTGGTGGTCACGCAGGGGAACCAGCTCGGCTGG
ACCAGCCCCGCCTCGCTCGGCCTCGTCGCGGCCACGGTCGTCTTCGGCTGGCTGTTCCTCCGGGTCGAGCGCCGGGCCCG
CAGCCCCTTCATCGACTTCGGCCTGTTCCGGAGCTCGACCTACACCGGCGCGACCGTCTCGAACTTCCTGCTGAACGGCG
TCGCCGGGACGCTGATCGTGTCGCTGCAGCTGGTGCAGGTCGGCGGCGGCATGACCGCCCAGCGCGCCGGCGTCCTGACG
CTGGGCTACGCCGTCGCCATCATCGCGTTCATCCGCGTGGGCGAGAAGCTGCTCCGGCGCTTCGGCGCGCGCCCGCCCAT
GCTCTGGGGCTGCGTCATCACCGGCGCCTCGATCCTGCTGCTCGCGCCCTCCAACGTGATGCTGGCGCGGTACGAGGCGA
TGGCCGTGATCGGCTACACGTTGTTCGGCGTCGGGCTTGCGTTCTACGCGACGCCCTCCACCGACGCGGCGCTCTCGAAC
CTGCCCGCGTCGCAGGCCGGCGCCGGGTCCGGTCTCTACAAGATGGCCTCGTCCCTCGGCGCCGCGTTCGGCGTCGCGAT
CTCCGCGGCGATCTTCACCGCGCTCCGCGCCGCGCCGCAGAGCGTGCGCTGGCTCGAGGGCGTGATCACGTTCCAGGGCC
GGCAGGACAACCTCGCGGTGCGCGAGGCCGCCATCGTCGCGCTCGGCTTCAACGAGCTGATGGTCGTCGTCGCGATCGTC
TCGATCGTCCTCACCATCCCGCGGGGCGAGCGGGGCCAGGGCGCGTGA

Upstream 100 bases:

>100_bases
CCCCGCCGCGACCCGGGTCGTGCCCCTCGCCCGGCCGCTTCCCCAAAGAGCCGCGCCTCGGTGCTGGACGCCGCTGCGGC
GCGCGACGACCGTCGTCGGC

Downstream 100 bases:

>100_bases
GCGCCTCGCCCGCGTCCGCGCGCGCCAGCCGCACGATGGTGGTGATGCTCGCGGCGGCGATGAGCGCCTCGAGCACCGTG
CCGCCGACCGAGCCGGCGAG

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MDTTGTRTARDGVASFHGGDRLLLGIILGVLTFWLFAQTTLNIAPDMRRDLATGESVMNIAVALTALFSGIFIVVMGGLA
DRVGRVRMVRLGFLASIAGSLLVGLAPAGGLAVAFLLAGRALQGLSGACIMPASLALVKAYWDGAERQRAISLWSIGSWG
GSGVCSLFGGLVSQNLGWRWIFFGSVLVAVIGLLLLRGTPESRAEGGAADYRFDLAGVLTFMVAMVALQVVVTQGNQLGW
TSPASLGLVAATVVFGWLFLRVERRARSPFIDFGLFRSSTYTGATVSNFLLNGVAGTLIVSLQLVQVGGGMTAQRAGVLT
LGYAVAIIAFIRVGEKLLRRFGARPPMLWGCVITGASILLLAPSNVMLARYEAMAVIGYTLFGVGLAFYATPSTDAALSN
LPASQAGAGSGLYKMASSLGAAFGVAISAAIFTALRAAPQSVRWLEGVITFQGRQDNLAVREAAIVALGFNELMVVVAIV
SIVLTIPRGERGQGA

Sequences:

>Translated_495_residues
MDTTGTRTARDGVASFHGGDRLLLGIILGVLTFWLFAQTTLNIAPDMRRDLATGESVMNIAVALTALFSGIFIVVMGGLA
DRVGRVRMVRLGFLASIAGSLLVGLAPAGGLAVAFLLAGRALQGLSGACIMPASLALVKAYWDGAERQRAISLWSIGSWG
GSGVCSLFGGLVSQNLGWRWIFFGSVLVAVIGLLLLRGTPESRAEGGAADYRFDLAGVLTFMVAMVALQVVVTQGNQLGW
TSPASLGLVAATVVFGWLFLRVERRARSPFIDFGLFRSSTYTGATVSNFLLNGVAGTLIVSLQLVQVGGGMTAQRAGVLT
LGYAVAIIAFIRVGEKLLRRFGARPPMLWGCVITGASILLLAPSNVMLARYEAMAVIGYTLFGVGLAFYATPSTDAALSN
LPASQAGAGSGLYKMASSLGAAFGVAISAAIFTALRAAPQSVRWLEGVITFQGRQDNLAVREAAIVALGFNELMVVVAIV
SIVLTIPRGERGQGA
>Mature_495_residues
MDTTGTRTARDGVASFHGGDRLLLGIILGVLTFWLFAQTTLNIAPDMRRDLATGESVMNIAVALTALFSGIFIVVMGGLA
DRVGRVRMVRLGFLASIAGSLLVGLAPAGGLAVAFLLAGRALQGLSGACIMPASLALVKAYWDGAERQRAISLWSIGSWG
GSGVCSLFGGLVSQNLGWRWIFFGSVLVAVIGLLLLRGTPESRAEGGAADYRFDLAGVLTFMVAMVALQVVVTQGNQLGW
TSPASLGLVAATVVFGWLFLRVERRARSPFIDFGLFRSSTYTGATVSNFLLNGVAGTLIVSLQLVQVGGGMTAQRAGVLT
LGYAVAIIAFIRVGEKLLRRFGARPPMLWGCVITGASILLLAPSNVMLARYEAMAVIGYTLFGVGLAFYATPSTDAALSN
LPASQAGAGSGLYKMASSLGAAFGVAISAAIFTALRAAPQSVRWLEGVITFQGRQDNLAVREAAIVALGFNELMVVVAIV
SIVLTIPRGERGQGA

Specific function: Multidrug efflux pump that acts independently of norA and is one of the factors that confers resistance against diverse quinolones and chemical compounds [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=397, Percent_Identity=26.448362720403, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789042, Length=445, Percent_Identity=24.7191011235955, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788710, Length=374, Percent_Identity=24.331550802139, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324954, Length=218, Percent_Identity=26.605504587156, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51615; Mature: 51615

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCCCCCC
>Mature Secondary Structure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HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA