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The map label for this gene is zraS [H]

Identifier: 220915684

GI number: 220915684

Start: 644183

End: 644896

Strand: Direct

Name: zraS [H]

Synonym: A2cp1_0565

Alternate gene names: 220915684

Gene position: 644183-644896 (Clockwise)

Preceding gene: 220915683

Following gene: 220915688

Centisome position: 12.81

GC content: 77.59

Gene sequence:

>714_bases
ATGAGGGACGCGAGGGAACCGGGCGAGCCAGCGGCGGCGGGCGGCGCGGACGGGGCCGCATCCCGCGGCGAGCGCCTCGA
CGGCGCGACGCTCGCGCACGAGCTGAAGAACCCGCTCACGGCGGTGAAGGCGCTGGTGCAGCTCGGGCTGGCCAACCCGC
GAGAGGCCGCGTCGCACGAGCGGCTGGAGCAGGTGGCGCGGGCCGTCGAGCGGATGGAGCGGATCCTGCGCGACTACCTC
GCCTCGGCGCGCGGGATGGCGGAGCTCGCCCCGGCGCAGGTCGAGGTCGGCCCGCTCGTCACCGGCGTGCTGCAGGGGCT
CTCGGCGCGCGCGAGCGAGGCGCGGGTGCGCCTGGTGGCGCGCGGCGACGCGACGGTCGAGGCCGATCCGCGGCGGCTCG
AGGAGGCGCTCGTCAACCTGGTCGCGAACGGGATCGAGGCCACCCCGCCGGGCGGGGAGGTCGCGGTGGAGGTGCTGTCG
TCGGCGGAGGGCGCCGAGCTGGTGGTGCGCGACACGGGGAGCGGCATCACGGCCGACGCGCTCGAGCGGCTGGGCACGCC
GTTCTTCAGCACCCGGTCCGGGGGGAACGGGCTGGGCGTCGCGCACGTGCGGTCGGTGATCGCGATGCACGGCGGCTCGC
TCCGCTACGAGAGCGAGCCCGGGAAGGGCACGGTGGTCCGGGCCACGCTGCCCCGGCGCGCCCGGGCCGCCTGA

Upstream 100 bases:

>100_bases
TTGCGCGGGCCCTGCGCCAACCTGCGCGTTCAGCCGGCGCGCGCCCGCTGGTATCTCCCCGTCGTCCGCGACCGCGGGCG
ACGGGGAGGAGGTCGCAGCC

Downstream 100 bases:

>100_bases
GGCGCTTCAGAGCAGCGCGGCCGCCGCGGCTGCGAGGACCGCCACGGCGGCGCCGGCGCACCAGTCGCGGGCGCGGGGCG
GGGGCGCACCGGCCCACGCC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRDAREPGEPAAAGGADGAASRGERLDGATLAHELKNPLTAVKALVQLGLANPREAASHERLEQVARAVERMERILRDYL
ASARGMAELAPAQVEVGPLVTGVLQGLSARASEARVRLVARGDATVEADPRRLEEALVNLVANGIEATPPGGEVAVEVLS
SAEGAELVVRDTGSGITADALERLGTPFFSTRSGGNGLGVAHVRSVIAMHGGSLRYESEPGKGTVVRATLPRRARAA

Sequences:

>Translated_237_residues
MRDAREPGEPAAAGGADGAASRGERLDGATLAHELKNPLTAVKALVQLGLANPREAASHERLEQVARAVERMERILRDYL
ASARGMAELAPAQVEVGPLVTGVLQGLSARASEARVRLVARGDATVEADPRRLEEALVNLVANGIEATPPGGEVAVEVLS
SAEGAELVVRDTGSGITADALERLGTPFFSTRSGGNGLGVAHVRSVIAMHGGSLRYESEPGKGTVVRATLPRRARAA
>Mature_237_residues
MRDAREPGEPAAAGGADGAASRGERLDGATLAHELKNPLTAVKALVQLGLANPREAASHERLEQVARAVERMERILRDYL
ASARGMAELAPAQVEVGPLVTGVLQGLSARASEARVRLVARGDATVEADPRRLEEALVNLVANGIEATPPGGEVAVEVLS
SAEGAELVVRDTGSGITADALERLGTPFFSTRSGGNGLGVAHVRSVIAMHGGSLRYESEPGKGTVVRATLPRRARAA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=207, Percent_Identity=32.3671497584541, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788549, Length=220, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=29.4930875576037, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=26.6375545851528, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790346, Length=220, Percent_Identity=27.7272727272727, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786783, Length=219, Percent_Identity=29.2237442922374, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1790300, Length=221, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789149, Length=213, Percent_Identity=27.2300469483568, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=200, Percent_Identity=31.5, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=221, Percent_Identity=23.9819004524887, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87081816, Length=222, Percent_Identity=28.3783783783784, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 24557; Mature: 24557

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDAREPGEPAAAGGADGAASRGERLDGATLAHELKNPLTAVKALVQLGLANPREAASHE
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RLEQVARAVERMERILRDYLASARGMAELAPAQVEVGPLVTGVLQGLSARASEARVRLVA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHEEEEE
RGDATVEADPRRLEEALVNLVANGIEATPPGGEVAVEVLSSAEGAELVVRDTGSGITADA
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHH
LERLGTPFFSTRSGGNGLGVAHVRSVIAMHGGSLRYESEPGKGTVVRATLPRRARAA
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MRDAREPGEPAAAGGADGAASRGERLDGATLAHELKNPLTAVKALVQLGLANPREAASHE
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RLEQVARAVERMERILRDYLASARGMAELAPAQVEVGPLVTGVLQGLSARASEARVRLVA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHEEEEE
RGDATVEADPRRLEEALVNLVANGIEATPPGGEVAVEVLSSAEGAELVVRDTGSGITADA
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHH
LERLGTPFFSTRSGGNGLGVAHVRSVIAMHGGSLRYESEPGKGTVVRATLPRRARAA
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]