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The map label for this gene is hybD [H]

Identifier: 220915624

GI number: 220915624

Start: 580581

End: 581108

Strand: Reverse

Name: hybD [H]

Synonym: A2cp1_0505

Alternate gene names: 220915624

Gene position: 581108-580581 (Counterclockwise)

Preceding gene: 220915625

Following gene: 220915623

Centisome position: 11.55

GC content: 76.33

Gene sequence:

>528_bases
GTGGCGCGCATCGCGGTGTTCGGCATCGGCAACGTGCTCACCGGCGACGACGCCGTCGGGCCATACGTGGTGAAGGTGCT
GGAGGCGGGCTGGGAGCTGCCCGGCGTCGAGGTGGTGGACGCCGGCACGCCCGGCCTCGACCTCACCGCCTACTTCGCGG
GCCTCGACGCGGCCGTCCTGGTGGACGCGGTGAAGGCGCGCGCCGCGCCGGGCGAGCTGCGGACCTACTCGCGCGAGGAC
CTGGTGAAGCGCTCGCCGGTGCTGGCCGTGAGCCCCCACGAGCCCGGGCTGCGCGAGGCGGTGATGAACGCGGAGTTCAT
GGGCGTGTGCCCGGCCACGGTGCGCCTGGTGGGCGTGGTGGCGGAGCGGACCGATCTCGGCTGCGCGCTCTCCCCGGCGG
TGCAGGCGGCGGTGCCGGCGGCGGTCGAGCAGGTGCTCTCCGAGCTCCGGGCGCTGGGCGTCGCGCCGCGACCCCGCGAC
CCACCCGCCGAGCCGGACCTGTGGTGGGAGAAGGGCCGCGCCGGCTAG

Upstream 100 bases:

>100_bases
GTAGCTTCCTGCGTCGAGTGGTACACCCGATCCCGTCCTCGACCGCCGAGGACGGGATCGGTCTTTTCGGAGGCCCCGCC
GCGCGGGGAGGAGGCCCGGG

Downstream 100 bases:

>100_bases
CGCCGGGCCCGCCCGCAGGGAATGCGCGACGGGCAAGCGGACGTTGATCGACCGGGTGGCCGCCGCGCGTTGCGGCGGCG
AAACGCGCGGCGGTAAGCTC

Product: hydrogenase maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MARIAVFGIGNVLTGDDAVGPYVVKVLEAGWELPGVEVVDAGTPGLDLTAYFAGLDAAVLVDAVKARAAPGELRTYSRED
LVKRSPVLAVSPHEPGLREAVMNAEFMGVCPATVRLVGVVAERTDLGCALSPAVQAAVPAAVEQVLSELRALGVAPRPRD
PPAEPDLWWEKGRAG

Sequences:

>Translated_175_residues
MARIAVFGIGNVLTGDDAVGPYVVKVLEAGWELPGVEVVDAGTPGLDLTAYFAGLDAAVLVDAVKARAAPGELRTYSRED
LVKRSPVLAVSPHEPGLREAVMNAEFMGVCPATVRLVGVVAERTDLGCALSPAVQAAVPAAVEQVLSELRALGVAPRPRD
PPAEPDLWWEKGRAG
>Mature_174_residues
ARIAVFGIGNVLTGDDAVGPYVVKVLEAGWELPGVEVVDAGTPGLDLTAYFAGLDAAVLVDAVKARAAPGELRTYSREDL
VKRSPVLAVSPHEPGLREAVMNAEFMGVCPATVRLVGVVAERTDLGCALSPAVQAAVPAAVEQVLSELRALGVAPRPRDP
PAEPDLWWEKGRAG

Specific function: Protease involved in the C-terminal processing of hybC, the large subunit of hydrogenase 2. Specifically cleaves off a 15 amino acid peptide from the C-terminus of the precursor of hybC [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=163, Percent_Identity=37.4233128834356, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1787209, Length=158, Percent_Identity=34.8101265822785, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 18245; Mature: 18114

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIAVFGIGNVLTGDDAVGPYVVKVLEAGWELPGVEVVDAGTPGLDLTAYFAGLDAAVL
CCEEEEEECCCEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCHHHHH
VDAVKARAAPGELRTYSREDLVKRSPVLAVSPHEPGLREAVMNAEFMGVCPATVRLVGVV
HHHHHHCCCCCHHHHCCHHHHHHHCCEEEECCCCCCHHHHHHCCHHCCCCHHHHHHHHHH
AERTDLGCALSPAVQAAVPAAVEQVLSELRALGVAPRPRDPPAEPDLWWEKGRAG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARIAVFGIGNVLTGDDAVGPYVVKVLEAGWELPGVEVVDAGTPGLDLTAYFAGLDAAVL
CEEEEEECCCEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCHHHHH
VDAVKARAAPGELRTYSREDLVKRSPVLAVSPHEPGLREAVMNAEFMGVCPATVRLVGVV
HHHHHHCCCCCHHHHCCHHHHHHHCCEEEECCCCCCHHHHHHCCHHCCCCHHHHHHHHHH
AERTDLGCALSPAVQAAVPAAVEQVLSELRALGVAPRPRDPPAEPDLWWEKGRAG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8021226; 9278503; 10331925 [H]