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The map label for this gene is hybC [H]

Identifier: 220915625

GI number: 220915625

Start: 581205

End: 582938

Strand: Reverse

Name: hybC [H]

Synonym: A2cp1_0506

Alternate gene names: 220915625

Gene position: 582938-581205 (Counterclockwise)

Preceding gene: 220915626

Following gene: 220915624

Centisome position: 11.59

GC content: 68.28

Gene sequence:

>1734_bases
ATGAGCAAGCTCATCACCATCGACCCGGTCACGCGCATCGAGGGTCACCTCCGCATCGACGTCGAGGTCGACGGCGGCGC
GGTCAAGAACGCCTGGTCCTCGGGCACCATGTGGCGCGGCATCGAGACCATCCTCCAGGGCCGCGATCCCCGCGACGCCT
GGATCTTCACGCAGCGCATCTGCGGCGTGTGCACCACCGTGCACGCGATCGCCTCGGTGCGCAGCGTCGAGAACGCGCTC
GGCCTCGAGATCCCGCTGAACGCGCAGCTCATCCGCAACCTCATCGTCACGGCGCACACGCTGCACGACCACATCGTGCA
CTTCTACCAGCTGTCCGCGCTGGACTGGGTGGACGTGACCAGCGCCCTGAAGGCCGACCCGGTGAAGGCGGCGCAGCTCG
CCGAGTCGATCTCCAGCTGGCCGGGCAACTCGAAGCACGAGCTCGCGGCGGTGAAGGCGCGCCTCGAGGGCTTCGTGAAG
GCCGGCCAGATGGGCATCTTCACCAACGGCTACTGGGGCCACCCGGCGATGAAGCTCTCGCCGGAGGTGAACCTGCTCGC
GGTCGCGCACTACCTCCAGGCGCTGGAGATCCAGCGCAAGGCCAACATGGCGGTGGCGATCCTCGGCTCGAAGACGCCGA
ACATCCAGAACCTGGCGGTGGGCGGCGTCGCCAACGCCATCAACCTGGACAGCCCGGCCGCGCTGAACATGGAGAAGCTG
TACCAGGTGAAGACCCTCATCGACGAGGTCGCCACCTTCATCCAGCAGTGCTACCTGCCCGACGTGGCCGCCATCGGCGG
CTTCTACCCGGACTGGTTCAAGTACGGCGCGGGGGTGACGAACTACCTCTCGGTGCCCGAGCTCCCGCTGGACGAGAAGA
TGTCGAAGTTCGACATCCCGGGCGGCGTGCTCTGGAACGGCAAGCTCGAGGTCGCGCACAAGATCACGAGCTGGAACGAC
GACTACCTGAAGCAGAACGTGACGGAGTCGATCGCGCACGCCCACTACGACGGCGCGTGGGCCAAGGGGCCGTGGGAGGA
GGACGACAAGCCGAAGTTCGCCGGTGAGTGGAAGCACGAGACGCCGGCGCCCGACCGCTACACCTGGGTGAAGGCGCCGC
GGCTCGAGGGCAAGGCGGTGCAGGTCGGGCCGCTCGCCTGCGTGCTGGTGGGCCTCGCCTCCGGCCACGAGCCCACCACC
CGGTGGGCCACCGCGGCCATCGGCGCGGTGAAGGCGGTCCACAAGATCGACCTCACCCCGGCCCACCTCCACTCCACGCT
CGGCCGCCACGCGGCGCGCGCCATCCGCGCGGCCATGATGGCCGAGATGGCCCAGAAGCAGTGGAAGCTCCTGGTCGAGA
ACATCGGCAAGGGCGACACGCGCATCTGGGTGGACCCGATGGCGAACCTGAAGCCCGGCATCTACAAGGGCGTCGGCTTC
CACGAGGCGCCGCGCGGCACGCTCTCGCACTGGTGCGTGATCGAGGCCGACAAGAAGGGCATGAAGCTGAAGAACTACCA
GGCCGTCGTGCCCTCGACCTGGAACGCCGGGCCCCGCGACGCGAGCGGCGCCCTCGGCCCCTACGAGAAGTCGCTCGTCG
GCAACCCCATCGCCGATCCGAAGCTGCCGCTCGAGGCGCTCCGCACGATCCACTCGTTCGACCCATGCCTCGCCTGCGCG
ATCCACACCGTGGACGCGGAGGGTGAGGAGATCTCGCGGGTGAAGGTGCTGTAG

Upstream 100 bases:

>100_bases
TCTCCCGCCTGTTCCCCGTCGTCGTCGTCGACCAGGGGAAGAAGTCCACGATCGGCCGCGGCGAGGTCCGGGCCGCTTCC
GGCCACTGAGGGGTGCCTAA

Downstream 100 bases:

>100_bases
CTTCCTGCGTCGAGTGGTACACCCGATCCCGTCCTCGACCGCCGAGGACGGGATCGGTCTTTTCGGAGGCCCCGCCGCGC
GGGGAGGAGGCCCGGGGTGG

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MSKLITIDPVTRIEGHLRIDVEVDGGAVKNAWSSGTMWRGIETILQGRDPRDAWIFTQRICGVCTTVHAIASVRSVENAL
GLEIPLNAQLIRNLIVTAHTLHDHIVHFYQLSALDWVDVTSALKADPVKAAQLAESISSWPGNSKHELAAVKARLEGFVK
AGQMGIFTNGYWGHPAMKLSPEVNLLAVAHYLQALEIQRKANMAVAILGSKTPNIQNLAVGGVANAINLDSPAALNMEKL
YQVKTLIDEVATFIQQCYLPDVAAIGGFYPDWFKYGAGVTNYLSVPELPLDEKMSKFDIPGGVLWNGKLEVAHKITSWND
DYLKQNVTESIAHAHYDGAWAKGPWEEDDKPKFAGEWKHETPAPDRYTWVKAPRLEGKAVQVGPLACVLVGLASGHEPTT
RWATAAIGAVKAVHKIDLTPAHLHSTLGRHAARAIRAAMMAEMAQKQWKLLVENIGKGDTRIWVDPMANLKPGIYKGVGF
HEAPRGTLSHWCVIEADKKGMKLKNYQAVVPSTWNAGPRDASGALGPYEKSLVGNPIADPKLPLEALRTIHSFDPCLACA
IHTVDAEGEEISRVKVL

Sequences:

>Translated_577_residues
MSKLITIDPVTRIEGHLRIDVEVDGGAVKNAWSSGTMWRGIETILQGRDPRDAWIFTQRICGVCTTVHAIASVRSVENAL
GLEIPLNAQLIRNLIVTAHTLHDHIVHFYQLSALDWVDVTSALKADPVKAAQLAESISSWPGNSKHELAAVKARLEGFVK
AGQMGIFTNGYWGHPAMKLSPEVNLLAVAHYLQALEIQRKANMAVAILGSKTPNIQNLAVGGVANAINLDSPAALNMEKL
YQVKTLIDEVATFIQQCYLPDVAAIGGFYPDWFKYGAGVTNYLSVPELPLDEKMSKFDIPGGVLWNGKLEVAHKITSWND
DYLKQNVTESIAHAHYDGAWAKGPWEEDDKPKFAGEWKHETPAPDRYTWVKAPRLEGKAVQVGPLACVLVGLASGHEPTT
RWATAAIGAVKAVHKIDLTPAHLHSTLGRHAARAIRAAMMAEMAQKQWKLLVENIGKGDTRIWVDPMANLKPGIYKGVGF
HEAPRGTLSHWCVIEADKKGMKLKNYQAVVPSTWNAGPRDASGALGPYEKSLVGNPIADPKLPLEALRTIHSFDPCLACA
IHTVDAEGEEISRVKVL
>Mature_576_residues
SKLITIDPVTRIEGHLRIDVEVDGGAVKNAWSSGTMWRGIETILQGRDPRDAWIFTQRICGVCTTVHAIASVRSVENALG
LEIPLNAQLIRNLIVTAHTLHDHIVHFYQLSALDWVDVTSALKADPVKAAQLAESISSWPGNSKHELAAVKARLEGFVKA
GQMGIFTNGYWGHPAMKLSPEVNLLAVAHYLQALEIQRKANMAVAILGSKTPNIQNLAVGGVANAINLDSPAALNMEKLY
QVKTLIDEVATFIQQCYLPDVAAIGGFYPDWFKYGAGVTNYLSVPELPLDEKMSKFDIPGGVLWNGKLEVAHKITSWNDD
YLKQNVTESIAHAHYDGAWAKGPWEEDDKPKFAGEWKHETPAPDRYTWVKAPRLEGKAVQVGPLACVLVGLASGHEPTTR
WATAAIGAVKAVHKIDLTPAHLHSTLGRHAARAIRAAMMAEMAQKQWKLLVENIGKGDTRIWVDPMANLKPGIYKGVGFH
EAPRGTLSHWCVIEADKKGMKLKNYQAVVPSTWNAGPRDASGALGPYEKSLVGNPIADPKLPLEALRTIHSFDPCLACAI
HTVDAEGEEISRVKVL

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=578, Percent_Identity=55.363321799308, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI1787207, Length=600, Percent_Identity=42.1666666666667, Blast_Score=450, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 63032; Mature: 62901

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLITIDPVTRIEGHLRIDVEVDGGAVKNAWSSGTMWRGIETILQGRDPRDAWIFTQRI
CCCEEEECCHHHCCCEEEEEEEECCCHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
CGVCTTVHAIASVRSVENALGLEIPLNAQLIRNLIVTAHTLHDHIVHFYQLSALDWVDVT
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SALKADPVKAAQLAESISSWPGNSKHELAAVKARLEGFVKAGQMGIFTNGYWGHPAMKLS
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEC
PEVNLLAVAHYLQALEIQRKANMAVAILGSKTPNIQNLAVGGVANAINLDSPAALNMEKL
CCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCHHHH
YQVKTLIDEVATFIQQCYLPDVAAIGGFYPDWFKYGAGVTNYLSVPELPLDEKMSKFDIP
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHCCCCHHCCCCCCCCHHHHHHHCCCC
GGVLWNGKLEVAHKITSWNDDYLKQNVTESIAHAHYDGAWAKGPWEEDDKPKFAGEWKHE
CCEEECCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
TPAPDRYTWVKAPRLEGKAVQVGPLACVLVGLASGHEPTTRWATAAIGAVKAVHKIDLTP
CCCCCCEEEEECCCCCCCEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCH
AHLHSTLGRHAARAIRAAMMAEMAQKQWKLLVENIGKGDTRIWVDPMANLKPGIYKGVGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCC
HEAPRGTLSHWCVIEADKKGMKLKNYQAVVPSTWNAGPRDASGALGPYEKSLVGNPIADP
CCCCCCCCCEEEEEEECCCCCEECCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
KLPLEALRTIHSFDPCLACAIHTVDAEGEEISRVKVL
CCCHHHHHHHHCCCHHHHHHEEECCCCCCCCCCEECC
>Mature Secondary Structure 
SKLITIDPVTRIEGHLRIDVEVDGGAVKNAWSSGTMWRGIETILQGRDPRDAWIFTQRI
CCEEEECCHHHCCCEEEEEEEECCCHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
CGVCTTVHAIASVRSVENALGLEIPLNAQLIRNLIVTAHTLHDHIVHFYQLSALDWVDVT
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SALKADPVKAAQLAESISSWPGNSKHELAAVKARLEGFVKAGQMGIFTNGYWGHPAMKLS
HHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEC
PEVNLLAVAHYLQALEIQRKANMAVAILGSKTPNIQNLAVGGVANAINLDSPAALNMEKL
CCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCHHHH
YQVKTLIDEVATFIQQCYLPDVAAIGGFYPDWFKYGAGVTNYLSVPELPLDEKMSKFDIP
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHCCCCHHCCCCCCCCHHHHHHHCCCC
GGVLWNGKLEVAHKITSWNDDYLKQNVTESIAHAHYDGAWAKGPWEEDDKPKFAGEWKHE
CCEEECCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
TPAPDRYTWVKAPRLEGKAVQVGPLACVLVGLASGHEPTTRWATAAIGAVKAVHKIDLTP
CCCCCCEEEEECCCCCCCEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCH
AHLHSTLGRHAARAIRAAMMAEMAQKQWKLLVENIGKGDTRIWVDPMANLKPGIYKGVGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCC
HEAPRGTLSHWCVIEADKKGMKLKNYQAVVPSTWNAGPRDASGALGPYEKSLVGNPIADP
CCCCCCCCCEEEEEEECCCCCEECCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
KLPLEALRTIHSFDPCLACAIHTVDAEGEEISRVKVL
CCCHHHHHHHHCCCHHHHHHEEECCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]