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The map label for this gene is prfA

Identifier: 220915515

GI number: 220915515

Start: 445475

End: 446587

Strand: Reverse

Name: prfA

Synonym: A2cp1_0396

Alternate gene names: 220915515

Gene position: 446587-445475 (Counterclockwise)

Preceding gene: 220915516

Following gene: 220915514

Centisome position: 8.88

GC content: 70.71

Gene sequence:

>1113_bases
ATGCTGTCCCCCGACGTCCTGAAGAAGCTCGAGGCCATCGAGCAGCGGTTCGAGGAGCTCACCCAGCTCCTCTCGGACCC
GGCCGTGGCCGGCAACGGCGACCGGTTCCGGAAGGTGGCGAAGGAGCGCGCCTCCATCGAGCAGACCGTCACCGCCCTGC
GCGCCTATCGCAAGCTGCTCGAGGACGTGGAGGGCAACGAGGCGCTGCTCGCGGACAAGGACCCCGAGCTGCGCGAGCTC
GCCAAGGAGGAGCTGGCGCAGCTGCGGCCGCAGGTCGATCCCGCCGAGGAGCAGCTCAAGCTGTACCTCGTGCCGAAGGA
CCCGAACGACGAGAAGGACGTCATCGTCGAGATCCGGGCCGGCGCGGGCGGCGACGAGGCGGGGCTGTTCGCGGCCGAGG
TCATGCGCATGTACGTCCGCTACGCCGAGCGCCGCGGCTGGCGCGTCGAGCTGATGGACACCTCGGGCGGCGCGCTCGGC
GGCGTGAAGGAGGCGACGCTCACCGTCTCCGGCGACGCGGTCTACTCCTCGCTCAAGTACGAGTCCGGCGTGCACCGGGT
GCAGCGCGTCCCCGCCACCGAGGCCCAGGGGCGCATCCACACCTCCACCGTCACCGTGGCGGTGATGCCCGAGGCGGAGG
AGATCGACGTCCAGGTGAACCCGGCCGACGTGGAGATGGACGTGTTCCGCTCCACCGGCTCGGGCGGCCAGTCGGTGAAC
ACCACCGACTCCGCGGTGCGCCTCACCCACAAGCCGACCGGCATCGTGGTGAAGTGCCAGCAGGAGAAGAGCCAGCTGAA
GAACCGCACCATGGCCATGAAGATGCTGCGGGCCAAGCTGTTCGAGATCGAGCAGGAGCGGCAGCGCAGCGCGCGCGACG
CGACCCGCAAGTCGCAGGTGGGCACCGGCGACCGCTCCGAGAAGATCCGCACCTACAACTTCCCGCAGGACCGGCTCACC
GACCACCGCGTGAACTACACGCGCCACAACCTGCCCGCGGTGATGGACGGCGACGTGCAGGACGTCATCGACGCCTGCCG
GACGTTCTACGCGGCCCAGGCGCTGCGCGAGGCGTCGCGCGGCGAGGCCGGGGCGGCGGAGCGGCGGGCGTGA

Upstream 100 bases:

>100_bases
CGGCGCTTCGCCCGCCCCGGGCGCGGCCGGGCGGCCGCGGACCACCCGGCGGCCCGATGCCTTGCCCATCCCCGCGTGGA
AACGGTAAGAAGTCCCCCCC

Downstream 100 bases:

>100_bases
GCGACGGCGGGGCCTGGACGACGCTCCGCCTCCTCGCCTGGACCCAGGAGTTCTTCGGCCGCAAGGGCGTGGACGCGCCG
CGGCTCACCGCGGAGCTGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MLSPDVLKKLEAIEQRFEELTQLLSDPAVAGNGDRFRKVAKERASIEQTVTALRAYRKLLEDVEGNEALLADKDPELREL
AKEELAQLRPQVDPAEEQLKLYLVPKDPNDEKDVIVEIRAGAGGDEAGLFAAEVMRMYVRYAERRGWRVELMDTSGGALG
GVKEATLTVSGDAVYSSLKYESGVHRVQRVPATEAQGRIHTSTVTVAVMPEAEEIDVQVNPADVEMDVFRSTGSGGQSVN
TTDSAVRLTHKPTGIVVKCQQEKSQLKNRTMAMKMLRAKLFEIEQERQRSARDATRKSQVGTGDRSEKIRTYNFPQDRLT
DHRVNYTRHNLPAVMDGDVQDVIDACRTFYAAQALREASRGEAGAAERRA

Sequences:

>Translated_370_residues
MLSPDVLKKLEAIEQRFEELTQLLSDPAVAGNGDRFRKVAKERASIEQTVTALRAYRKLLEDVEGNEALLADKDPELREL
AKEELAQLRPQVDPAEEQLKLYLVPKDPNDEKDVIVEIRAGAGGDEAGLFAAEVMRMYVRYAERRGWRVELMDTSGGALG
GVKEATLTVSGDAVYSSLKYESGVHRVQRVPATEAQGRIHTSTVTVAVMPEAEEIDVQVNPADVEMDVFRSTGSGGQSVN
TTDSAVRLTHKPTGIVVKCQQEKSQLKNRTMAMKMLRAKLFEIEQERQRSARDATRKSQVGTGDRSEKIRTYNFPQDRLT
DHRVNYTRHNLPAVMDGDVQDVIDACRTFYAAQALREASRGEAGAAERRA
>Mature_370_residues
MLSPDVLKKLEAIEQRFEELTQLLSDPAVAGNGDRFRKVAKERASIEQTVTALRAYRKLLEDVEGNEALLADKDPELREL
AKEELAQLRPQVDPAEEQLKLYLVPKDPNDEKDVIVEIRAGAGGDEAGLFAAEVMRMYVRYAERRGWRVELMDTSGGALG
GVKEATLTVSGDAVYSSLKYESGVHRVQRVPATEAQGRIHTSTVTVAVMPEAEEIDVQVNPADVEMDVFRSTGSGGQSVN
TTDSAVRLTHKPTGIVVKCQQEKSQLKNRTMAMKMLRAKLFEIEQERQRSARDATRKSQVGTGDRSEKIRTYNFPQDRLT
DHRVNYTRHNLPAVMDGDVQDVIDACRTFYAAQALREASRGEAGAAERRA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=311, Percent_Identity=45.9807073954984, Blast_Score=296, Evalue=1e-80,
Organism=Homo sapiens, GI34577120, Length=337, Percent_Identity=39.1691394658754, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI166795305, Length=205, Percent_Identity=45.3658536585366, Blast_Score=192, Evalue=6e-49,
Organism=Escherichia coli, GI1787462, Length=344, Percent_Identity=49.1279069767442, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI2367172, Length=351, Percent_Identity=39.031339031339, Blast_Score=234, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17542784, Length=293, Percent_Identity=36.518771331058, Blast_Score=190, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=366, Percent_Identity=37.9781420765027, Blast_Score=239, Evalue=3e-64,
Organism=Drosophila melanogaster, GI19921226, Length=315, Percent_Identity=43.8095238095238, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_ANAD2 (B8JAU2)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002490819.1
- ProteinModelPortal:   B8JAU2
- SMR:   B8JAU2
- GeneID:   7299279
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0396
- HOGENOM:   HBG629764
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41159; Mature: 41159

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPDVLKKLEAIEQRFEELTQLLSDPAVAGNGDRFRKVAKERASIEQTVTALRAYRKLL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDVEGNEALLADKDPELRELAKEELAQLRPQVDPAEEQLKLYLVPKDPNDEKDVIVEIRA
HHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEC
GAGGDEAGLFAAEVMRMYVRYAERRGWRVELMDTSGGALGGVKEATLTVSGDAVYSSLKY
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHH
ESGVHRVQRVPATEAQGRIHTSTVTVAVMPEAEEIDVQVNPADVEMDVFRSTGSGGQSVN
HHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCEEEEECCCCCCHHHHHCCCCCCCCCC
TTDSAVRLTHKPTGIVVKCQQEKSQLKNRTMAMKMLRAKLFEIEQERQRSARDATRKSQV
CCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTGDRSEKIRTYNFPQDRLTDHRVNYTRHNLPAVMDGDVQDVIDACRTFYAAQALREASR
CCCCCCCCCEECCCCHHHHCCCCCCCHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHC
GEAGAAERRA
CCCCCCCCCC
>Mature Secondary Structure
MLSPDVLKKLEAIEQRFEELTQLLSDPAVAGNGDRFRKVAKERASIEQTVTALRAYRKLL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDVEGNEALLADKDPELRELAKEELAQLRPQVDPAEEQLKLYLVPKDPNDEKDVIVEIRA
HHCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEC
GAGGDEAGLFAAEVMRMYVRYAERRGWRVELMDTSGGALGGVKEATLTVSGDAVYSSLKY
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHH
ESGVHRVQRVPATEAQGRIHTSTVTVAVMPEAEEIDVQVNPADVEMDVFRSTGSGGQSVN
HHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCEEEEECCCCCCHHHHHCCCCCCCCCC
TTDSAVRLTHKPTGIVVKCQQEKSQLKNRTMAMKMLRAKLFEIEQERQRSARDATRKSQV
CCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTGDRSEKIRTYNFPQDRLTDHRVNYTRHNLPAVMDGDVQDVIDACRTFYAAQALREASR
CCCCCCCCCEECCCCHHHHCCCCCCCHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHC
GEAGAAERRA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA