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The map label for this gene is purH [H]

Identifier: 220905525

GI number: 220905525

Start: 2724566

End: 2725843

Strand: Reverse

Name: purH [H]

Synonym: Ddes_2264

Alternate gene names: 220905525

Gene position: 2725843-2724566 (Counterclockwise)

Preceding gene: 220905530

Following gene: 220905524

Centisome position: 94.86

GC content: 60.02

Gene sequence:

>1278_bases
ATGTCGGATCTCAAGGCCATGTACAGCACTGTCCGCAAGGACGCTTTTCCCGAAACCATGACCATCATTCTTGGCGATGA
AAAACTCGTGTTCCAGAAGCGTGTCTGGACGCTGGACGGCGAGGAAAAGGGCCTGCGCTATGGGGAAAACCCCGATCAGC
CCGCAGCCCTGTACGCCCTGAAAGAGGGTTCCATCACTTGCGGCGGCCTCCAGTGGCGGGGTCCGGGGCACGGCATTGTT
TCTGCCCTGACCGAAGAGCAGATGATCCAGGCGGGCAAGCACCCCGGCAAGACCAACCTGACGGACGTGGACAACGGGGC
CAATATTCTTCAGTACCTCGCAGACCGCCCGGCGGCAGTCATTCTGAAGCACAATAATCCCTGCGGCGCGGCCTGGAGCA
GGGACGGCGTGGCCACCGCCCTCGAAAAAGCCTTCTGGTGCGACCGTATCGCGGCTTTTGGCGGCGCGGTGGTCGTCAAC
CGTTCCTTCACCCGTGAGGCCGCCGAAATGGTAGCCGCCAACTATTTTGAAGTGGTAGCTGCTCCGGCTTTTGAAGAAGG
CGCGGTGGAGATTCTTAAAAGCCGAAAAAACCTGCGCATTATGGAACTGCCCGGCCTGGGCCGGCTTGAGGAACTGACCT
CGTCGGCGTTTCTCGACATAAAGAGCCTGTCCGACGGCGGTATTGTAGTGCAGAAGTCTTTTGTAAACCGTGTTCTTTCC
GGCGACGATTTTTTGCCCGCCACCGCCACCACAAGGGACGGCCTTTCTGTGGCGGCACGTGCGCCAAGCCCGGCTGAGCT
GGATGACCTGCGCTTTGCCTGGGCCGTGGAGGCCGGTGTCACCTCCAACTCTGTCATTTTTGTGCGTGACGGGGCCACCG
TGGCTATCGGCACGGGTGAACAGGATCGCGTGGGCTGTGTAGAGCTTGCCATACACAAGGCGCACACCAAGTATGCGGAT
ACACTTGCCTTCCGTGAACTGGGCCTGACTCTTTACGAGCTGAAGCTCAAAGCCGCCGAAGACAGCACCCTGGCGGAAAA
GCTGGCCGACATCGAGCGGCGCACAGAGGAAACCCACGGCGGCCTTGCCGGCACGGCCCTGGTGTCCGATGGTTTTTTCC
CCTTTCGCGACGGCGTGGATGTAGCCATTGCCCAGGGCGTTACGGCCATAGCCCAGCCAGGCGGATCCATGCGTGACGCC
GAAGTCATCATGGCCTGCAATGAGGCTGCTCCGCAGGTAGCCATGGTTTTTACGGGGCAGCGTTCTTTCAAGCATTAA

Upstream 100 bases:

>100_bases
GCCGCGCCGCGCCGCATGGGCTGCGGATTTGGACTGGTGGGGCGGCTTCTTCTGTGCTATGCAAAACCCTTACCTGACCG
GCGAACATCTGGAGGCAAGC

Downstream 100 bases:

>100_bases
GCCCGGTGGCATCGGGCCGGATCGCGCCAAGGCGCGGTCCGGCCCTGCCGCCTGTATTTTCCCGGTCTGCCCCGGCCCGT
GCGGCAGACTGGTCATGCCC

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSDLKAMYSTVRKDAFPETMTIILGDEKLVFQKRVWTLDGEEKGLRYGENPDQPAALYALKEGSITCGGLQWRGPGHGIV
SALTEEQMIQAGKHPGKTNLTDVDNGANILQYLADRPAAVILKHNNPCGAAWSRDGVATALEKAFWCDRIAAFGGAVVVN
RSFTREAAEMVAANYFEVVAAPAFEEGAVEILKSRKNLRIMELPGLGRLEELTSSAFLDIKSLSDGGIVVQKSFVNRVLS
GDDFLPATATTRDGLSVAARAPSPAELDDLRFAWAVEAGVTSNSVIFVRDGATVAIGTGEQDRVGCVELAIHKAHTKYAD
TLAFRELGLTLYELKLKAAEDSTLAEKLADIERRTEETHGGLAGTALVSDGFFPFRDGVDVAIAQGVTAIAQPGGSMRDA
EVIMACNEAAPQVAMVFTGQRSFKH

Sequences:

>Translated_425_residues
MSDLKAMYSTVRKDAFPETMTIILGDEKLVFQKRVWTLDGEEKGLRYGENPDQPAALYALKEGSITCGGLQWRGPGHGIV
SALTEEQMIQAGKHPGKTNLTDVDNGANILQYLADRPAAVILKHNNPCGAAWSRDGVATALEKAFWCDRIAAFGGAVVVN
RSFTREAAEMVAANYFEVVAAPAFEEGAVEILKSRKNLRIMELPGLGRLEELTSSAFLDIKSLSDGGIVVQKSFVNRVLS
GDDFLPATATTRDGLSVAARAPSPAELDDLRFAWAVEAGVTSNSVIFVRDGATVAIGTGEQDRVGCVELAIHKAHTKYAD
TLAFRELGLTLYELKLKAAEDSTLAEKLADIERRTEETHGGLAGTALVSDGFFPFRDGVDVAIAQGVTAIAQPGGSMRDA
EVIMACNEAAPQVAMVFTGQRSFKH
>Mature_424_residues
SDLKAMYSTVRKDAFPETMTIILGDEKLVFQKRVWTLDGEEKGLRYGENPDQPAALYALKEGSITCGGLQWRGPGHGIVS
ALTEEQMIQAGKHPGKTNLTDVDNGANILQYLADRPAAVILKHNNPCGAAWSRDGVATALEKAFWCDRIAAFGGAVVVNR
SFTREAAEMVAANYFEVVAAPAFEEGAVEILKSRKNLRIMELPGLGRLEELTSSAFLDIKSLSDGGIVVQKSFVNRVLSG
DDFLPATATTRDGLSVAARAPSPAELDDLRFAWAVEAGVTSNSVIFVRDGATVAIGTGEQDRVGCVELAIHKAHTKYADT
LAFRELGLTLYELKLKAAEDSTLAEKLADIERRTEETHGGLAGTALVSDGFFPFRDGVDVAIAQGVTAIAQPGGSMRDAE
VIMACNEAAPQVAMVFTGQRSFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=437, Percent_Identity=27.9176201372998, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1790439, Length=385, Percent_Identity=31.6883116883117, Blast_Score=158, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI71985564, Length=458, Percent_Identity=25.1091703056769, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24649832, Length=433, Percent_Identity=26.7898383371824, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 45594; Mature: 45463

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLKAMYSTVRKDAFPETMTIILGDEKLVFQKRVWTLDGEEKGLRYGENPDQPAALYAL
CCHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEE
KEGSITCGGLQWRGPGHGIVSALTEEQMIQAGKHPGKTNLTDVDNGANILQYLADRPAAV
ECCCEEECCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
ILKHNNPCGAAWSRDGVATALEKAFWCDRIAAFGGAVVVNRSFTREAAEMVAANYFEVVA
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHC
APAFEEGAVEILKSRKNLRIMELPGLGRLEELTSSAFLDIKSLSDGGIVVQKSFVNRVLS
CCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHEEEEEECCCCCEEEEHHHHHHHHC
GDDFLPATATTRDGLSVAARAPSPAELDDLRFAWAVEAGVTSNSVIFVRDGATVAIGTGE
CCCCCCCCCCCCCCCEEEECCCCCCCHHHCEEEEEEECCCCCCCEEEEECCCEEEEECCC
QDRVGCVELAIHKAHTKYADTLAFRELGLTLYELKLKAAEDSTLAEKLADIERRTEETHG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCC
GLAGTALVSDGFFPFRDGVDVAIAQGVTAIAQPGGSMRDAEVIMACNEAAPQVAMVFTGQ
CEEEEHHHCCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCCEEEEEECCCCCEEEEEEECC
RSFKH
CCCCC
>Mature Secondary Structure 
SDLKAMYSTVRKDAFPETMTIILGDEKLVFQKRVWTLDGEEKGLRYGENPDQPAALYAL
CHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEE
KEGSITCGGLQWRGPGHGIVSALTEEQMIQAGKHPGKTNLTDVDNGANILQYLADRPAAV
ECCCEEECCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
ILKHNNPCGAAWSRDGVATALEKAFWCDRIAAFGGAVVVNRSFTREAAEMVAANYFEVVA
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHC
APAFEEGAVEILKSRKNLRIMELPGLGRLEELTSSAFLDIKSLSDGGIVVQKSFVNRVLS
CCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHEEEEEECCCCCEEEEHHHHHHHHC
GDDFLPATATTRDGLSVAARAPSPAELDDLRFAWAVEAGVTSNSVIFVRDGATVAIGTGE
CCCCCCCCCCCCCCCEEEECCCCCCCHHHCEEEEEEECCCCCCCEEEEECCCEEEEECCC
QDRVGCVELAIHKAHTKYADTLAFRELGLTLYELKLKAAEDSTLAEKLADIERRTEETHG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCC
GLAGTALVSDGFFPFRDGVDVAIAQGVTAIAQPGGSMRDAEVIMACNEAAPQVAMVFTGQ
CEEEEHHHCCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCCEEEEEECCCCCEEEEEEECC
RSFKH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA