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The map label for this gene is yegE [H]

Identifier: 220905522

GI number: 220905522

Start: 2719436

End: 2720728

Strand: Reverse

Name: yegE [H]

Synonym: Ddes_2261

Alternate gene names: 220905522

Gene position: 2720728-2719436 (Counterclockwise)

Preceding gene: 220905523

Following gene: 220905521

Centisome position: 94.69

GC content: 53.75

Gene sequence:

>1293_bases
ATGCCCCCGCGCGATGTATCCCTTGTCTGCGGGAATCGTCTGGATTTCTTGCAGGGCCTGCCGCAGGCTCTTGCGGTTAT
GCGGGTAGACCTGGATGTTATGGGCAGGCCGCGTGATTTGATGGTTGTTTATGGCAATGAAGCGCTTTTTACACTTTTTG
GTGCTCCCCGTGAATGGATGACGGGCGTTTCGCTTGTTGACTCCTGTATGAAGCTGGACAGAAAATGGCTGGAAATTTTC
CGGAAGACTGCCTATCAGGGGCAAGTGCAGGAAATGACCGGTTTCTGGCCTTCGTTGGGCAGACACCTCGCTGTAACCTG
CCATCAGCCACAATACGGCTACTGTACCTGTCTTTTTCAGGATGTGACCGAGCGCGTACATAAAGAAAATGATCTGGTAA
AAAGCCGTAACCGTCTTGAAGCCCTGCTGCACAGCACTGTTGATATGGTGTTTCAGATTGATCCGGTTACGGGGGGCATC
AGCAATCTTGACCAGGAGCTGGCTGCCTGCGGCGGCCTGCTCAAAGCCAGAAGAGTTCCGGAAACCCTTCTGATGCAAGG
GCTTCTGGCTCCCGGCCAGAACGATAAAATCAGCAGCTTGATCAACCTTGCCCTGCGCGCTGAAAATGACTGTACCTGCG
AGGTGCGCGCACGGCTCAAGGCCGGTTCTCCCTATACCTGGCACAGTCTCACCGTTGTGGGCTACAGCGATCCGTGTTCT
AAAGAAATCGGTATAATAGGCTTTCTTAAAGACGTGCATGCCAGCATCAAGGAAAGGGACGCCCTGGTGCGCCGGGCAGA
AAAAGATCCTCTTTGCGGCATCTATAACAGGGCTGCCGGCGAAAACCTCGCTGCTTCCAGGCTGAGCGGAGCGGACGAAA
CCTGTCACAGCGCGGCCCTGCTTGTTTTTGATCTGGATAATTTCAAGGTAATTAACGACACGGCCGGGCATGCGGCGGGC
GATGCGGTGCTGAAAGCTTTTGCCGTTCTGCTGGGCCAGTGCTTTCGTAAAGACGATATTGTTTTTCGGTTTGGCGGGGA
TGAGTTTGCCGTTTTTGTGCAGAACATGCCGCAGGAAAGGATCATCCGTGTCAGTAAAAATATCGTGGCAAGGCTGGAAG
AACCGCTTGTTGACGATGTGCGCGTGGGTGCGTGTATTGGGGTTGCCTACAGCCGGTCGGGCCGCCACAGCTATGAAGAA
CTTTATAAAATGGCCGATAAGGCTTTGTACAATGCCAAAGAAGGCGGCAAGGGACAAACCGCGATTCTTTGTCTGCATGG
CAGCGGGGCCTGA

Upstream 100 bases:

>100_bases
CTGAAAGTTATTGTACCACGTTTTTCCAAAGCCCGGTTCCAGACGGCGCACGGGGCTTCCGATGAAAGCTGTGGATGGGT
GTTCTGCAGATTCTTTTCAG

Downstream 100 bases:

>100_bases
CGGGCTGTTGTGAGCTGGCCTGATGCGCCGGGTAGATTCCGGCGGCACAAAGGCGTACTGTTTTAAGGCGCTTTGCTGCG
CATCAAGGATTTGTGCGTCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPPRDVSLVCGNRLDFLQGLPQALAVMRVDLDVMGRPRDLMVVYGNEALFTLFGAPREWMTGVSLVDSCMKLDRKWLEIF
RKTAYQGQVQEMTGFWPSLGRHLAVTCHQPQYGYCTCLFQDVTERVHKENDLVKSRNRLEALLHSTVDMVFQIDPVTGGI
SNLDQELAACGGLLKARRVPETLLMQGLLAPGQNDKISSLINLALRAENDCTCEVRARLKAGSPYTWHSLTVVGYSDPCS
KEIGIIGFLKDVHASIKERDALVRRAEKDPLCGIYNRAAGENLAASRLSGADETCHSAALLVFDLDNFKVINDTAGHAAG
DAVLKAFAVLLGQCFRKDDIVFRFGGDEFAVFVQNMPQERIIRVSKNIVARLEEPLVDDVRVGACIGVAYSRSGRHSYEE
LYKMADKALYNAKEGGKGQTAILCLHGSGA

Sequences:

>Translated_430_residues
MPPRDVSLVCGNRLDFLQGLPQALAVMRVDLDVMGRPRDLMVVYGNEALFTLFGAPREWMTGVSLVDSCMKLDRKWLEIF
RKTAYQGQVQEMTGFWPSLGRHLAVTCHQPQYGYCTCLFQDVTERVHKENDLVKSRNRLEALLHSTVDMVFQIDPVTGGI
SNLDQELAACGGLLKARRVPETLLMQGLLAPGQNDKISSLINLALRAENDCTCEVRARLKAGSPYTWHSLTVVGYSDPCS
KEIGIIGFLKDVHASIKERDALVRRAEKDPLCGIYNRAAGENLAASRLSGADETCHSAALLVFDLDNFKVINDTAGHAAG
DAVLKAFAVLLGQCFRKDDIVFRFGGDEFAVFVQNMPQERIIRVSKNIVARLEEPLVDDVRVGACIGVAYSRSGRHSYEE
LYKMADKALYNAKEGGKGQTAILCLHGSGA
>Mature_429_residues
PPRDVSLVCGNRLDFLQGLPQALAVMRVDLDVMGRPRDLMVVYGNEALFTLFGAPREWMTGVSLVDSCMKLDRKWLEIFR
KTAYQGQVQEMTGFWPSLGRHLAVTCHQPQYGYCTCLFQDVTERVHKENDLVKSRNRLEALLHSTVDMVFQIDPVTGGIS
NLDQELAACGGLLKARRVPETLLMQGLLAPGQNDKISSLINLALRAENDCTCEVRARLKAGSPYTWHSLTVVGYSDPCSK
EIGIIGFLKDVHASIKERDALVRRAEKDPLCGIYNRAAGENLAASRLSGADETCHSAALLVFDLDNFKVINDTAGHAAGD
AVLKAFAVLLGQCFRKDDIVFRFGGDEFAVFVQNMPQERIIRVSKNIVARLEEPLVDDVRVGACIGVAYSRSGRHSYEEL
YKMADKALYNAKEGGKGQTAILCLHGSGA

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788381, Length=321, Percent_Identity=28.9719626168224, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=34.7826086956522, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=204, Percent_Identity=28.921568627451, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787541, Length=193, Percent_Identity=30.0518134715026, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=31.7919075144509, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=31.4465408805031, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=32.3353293413174, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788956, Length=157, Percent_Identity=33.7579617834395, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=123, Percent_Identity=39.0243902439024, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=27.3885350318471, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=26.8292682926829, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=32.1637426900585, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013655 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF05231 MASE1; PF08447 PAS_3; PF08448 PAS_4 [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 47414; Mature: 47283

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRDVSLVCGNRLDFLQGLPQALAVMRVDLDVMGRPRDLMVVYGNEALFTLFGAPREWM
CCCCCEEEECCCCHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCHHHH
TGVSLVDSCMKLDRKWLEIFRKTAYQGQVQEMTGFWPSLGRHLAVTCHQPQYGYCTCLFQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHCCCEEEEEECCCCCCHHHHHHH
DVTERVHKENDLVKSRNRLEALLHSTVDMVFQIDPVTGGISNLDQELAACGGLLKARRVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
ETLLMQGLLAPGQNDKISSLINLALRAENDCTCEVRARLKAGSPYTWHSLTVVGYSDPCS
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEEEECCCCCC
KEIGIIGFLKDVHASIKERDALVRRAEKDPLCGIYNRAAGENLAASRLSGADETCHSAAL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHCCCCCHHHHHEEE
LVFDLDNFKVINDTAGHAAGDAVLKAFAVLLGQCFRKDDIVFRFGGDEFAVFVQNMPQER
EEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCHHH
IIRVSKNIVARLEEPLVDDVRVGACIGVAYSRSGRHSYEELYKMADKALYNAKEGGKGQT
HHHHHHHHHHHHHHCCHHHHHHHHHEEEHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCE
AILCLHGSGA
EEEEEECCCC
>Mature Secondary Structure 
PPRDVSLVCGNRLDFLQGLPQALAVMRVDLDVMGRPRDLMVVYGNEALFTLFGAPREWM
CCCCEEEECCCCHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCHHHH
TGVSLVDSCMKLDRKWLEIFRKTAYQGQVQEMTGFWPSLGRHLAVTCHQPQYGYCTCLFQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHCCCEEEEEECCCCCCHHHHHHH
DVTERVHKENDLVKSRNRLEALLHSTVDMVFQIDPVTGGISNLDQELAACGGLLKARRVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
ETLLMQGLLAPGQNDKISSLINLALRAENDCTCEVRARLKAGSPYTWHSLTVVGYSDPCS
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEEEECCCCCC
KEIGIIGFLKDVHASIKERDALVRRAEKDPLCGIYNRAAGENLAASRLSGADETCHSAAL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHCCCCCHHHHHEEE
LVFDLDNFKVINDTAGHAAGDAVLKAFAVLLGQCFRKDDIVFRFGGDEFAVFVQNMPQER
EEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCHHH
IIRVSKNIVARLEEPLVDDVRVGACIGVAYSRSGRHSYEELYKMADKALYNAKEGGKGQT
HHHHHHHHHHHHHHCCHHHHHHHHHEEEHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCE
AILCLHGSGA
EEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 6094528; 7984428 [H]