Click here to switch to the map view.

The map label for this gene is proB [H]

Identifier: 220905508

GI number: 220905508

Start: 2707554

End: 2708864

Strand: Reverse

Name: proB [H]

Synonym: Ddes_2247

Alternate gene names: 220905508

Gene position: 2708864-2707554 (Counterclockwise)

Preceding gene: 220905509

Following gene: 220905506

Centisome position: 94.27

GC content: 63.84

Gene sequence:

>1311_bases
ATGAGCAGGCAGCGCCCTCATGGCACGGCGTCCGGAGCCGGACGGCCGGAGCAGTGCGGCACGTGTGGCTCTGAAGAAGA
GCGGTCTGAAACCTGGCAGCAGGAGCGCGTGCGCGTTCTGGCTCAGGCGCGGGTAATTGTGGTCAAGGTGGGCAGCGCCG
TGCTGACGGATGCCAGCGGGCTGAGCAGCCCTGTGCTGGCGGGGCTTGCCGCACAGCTTGCCGCCTTGCGCGACCTGCCC
CTTGTCAGTGATCCGGCGGGGCAGCCCCAGCAGGGGGCGCGCCGTCTGGTGCTGGTTTCTTCGGGCGCGGTGGCGGCCGG
GCGTGCCGCCCTGACAAGCCGGGGTCGCGTGGTGGAAACTGCGGGCCTGGCTGCGCGTCAGGCCGCGGCGGCTGTGGGGC
AGGGCCAGCTTATGCAGGCCTGGGACAAGGTTTTTCTGGCCCACGGCATGCCTACGGCACAGGTGCTGCTGACGCGTGAC
GACCTTCGCGCGCGTCAACGCTTTCTCAATGCCCGTAATACCTTTGCCGAATTGCTGCACTGGGATGTATTGCCGATCGT
CAATGAAAACGACACGGTATCCATCAGCGAACTGAAGTTTGGCGATAATGACTGTCTGGCGAGCCTGCTTATAAATCTGG
TGGAAGCCGATCTTTTTATCAATCTGACCTCTTCGTCGGGTGTGCTCGCGGCTGATCCGCAAAAATATCCCGATGCGCCC
GTTATGGACCATATCGACGATGTGGCAACGCTGGATCTGGGGCAGCTTTGCGGCGGCAAGACGCTGGTGGGCACAGGTGG
CATGTATTCCAAGCTGCTGGCCGCGCGCCGTGCCGCGCAACTGGGCGTACCCACACTGATTCTGCCCGGCCGTGAGCCTT
GGGTCATCAGCCGGGCCTTTGCCGCTGCCGGGGTATGCCCCGCGCCTGACGGGCATGAACCGTTCACGCGCGGAACCTGG
GTGCGGTCCGGGCAACACGCAATCACGCGGCGCAAGTTTTGGCTGGCCTATCAGTCTGACCCTGCGGGCAGCGTGCATGT
GGATGACGGTGCGGCCAAAGCCTTGCTGCACAAGGGCAGCAGTCTGCTGCCCGGCGGCGTGTGCGCGGTGGAAGGAACCT
TTCAGCGGGGGGGCCTTGTGCGCGTACTGCACAAGGGTGAAAGCATCGGTGTGGGACTTTCCAACTACAGTGCGCCGGAG
CTCAAGAAGATCATGGGGCTTAAGCGCCATGAGGTGGCGGCCATTCTTGGTGATGCCCATTACCCCGAAGTCATACACAG
GGATAACCTGCTGGTGGATGCGGCGGTCTGA

Upstream 100 bases:

>100_bases
AGCCGATTGTGCGTCTGGAAGGCCTCACCGATGTGGAGGAAGAGGAATTTCCCGAAATTGAAGTCATCTATACCCGCGAA
TAGCGGGATGTGGAGCGGAT

Downstream 100 bases:

>100_bases
TTCCGTGGGGCGTGTCCTCTGCTGCTGCGGGCGCGTCCGCATTTTGCATGATGCTCTGTCTTGACGCTGCGCAAAACGCC
GTAAGGCGATTGGCCTTGCC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSRQRPHGTASGAGRPEQCGTCGSEEERSETWQQERVRVLAQARVIVVKVGSAVLTDASGLSSPVLAGLAAQLAALRDLP
LVSDPAGQPQQGARRLVLVSSGAVAAGRAALTSRGRVVETAGLAARQAAAAVGQGQLMQAWDKVFLAHGMPTAQVLLTRD
DLRARQRFLNARNTFAELLHWDVLPIVNENDTVSISELKFGDNDCLASLLINLVEADLFINLTSSSGVLAADPQKYPDAP
VMDHIDDVATLDLGQLCGGKTLVGTGGMYSKLLAARRAAQLGVPTLILPGREPWVISRAFAAAGVCPAPDGHEPFTRGTW
VRSGQHAITRRKFWLAYQSDPAGSVHVDDGAAKALLHKGSSLLPGGVCAVEGTFQRGGLVRVLHKGESIGVGLSNYSAPE
LKKIMGLKRHEVAAILGDAHYPEVIHRDNLLVDAAV

Sequences:

>Translated_436_residues
MSRQRPHGTASGAGRPEQCGTCGSEEERSETWQQERVRVLAQARVIVVKVGSAVLTDASGLSSPVLAGLAAQLAALRDLP
LVSDPAGQPQQGARRLVLVSSGAVAAGRAALTSRGRVVETAGLAARQAAAAVGQGQLMQAWDKVFLAHGMPTAQVLLTRD
DLRARQRFLNARNTFAELLHWDVLPIVNENDTVSISELKFGDNDCLASLLINLVEADLFINLTSSSGVLAADPQKYPDAP
VMDHIDDVATLDLGQLCGGKTLVGTGGMYSKLLAARRAAQLGVPTLILPGREPWVISRAFAAAGVCPAPDGHEPFTRGTW
VRSGQHAITRRKFWLAYQSDPAGSVHVDDGAAKALLHKGSSLLPGGVCAVEGTFQRGGLVRVLHKGESIGVGLSNYSAPE
LKKIMGLKRHEVAAILGDAHYPEVIHRDNLLVDAAV
>Mature_435_residues
SRQRPHGTASGAGRPEQCGTCGSEEERSETWQQERVRVLAQARVIVVKVGSAVLTDASGLSSPVLAGLAAQLAALRDLPL
VSDPAGQPQQGARRLVLVSSGAVAAGRAALTSRGRVVETAGLAARQAAAAVGQGQLMQAWDKVFLAHGMPTAQVLLTRDD
LRARQRFLNARNTFAELLHWDVLPIVNENDTVSISELKFGDNDCLASLLINLVEADLFINLTSSSGVLAADPQKYPDAPV
MDHIDDVATLDLGQLCGGKTLVGTGGMYSKLLAARRAAQLGVPTLILPGREPWVISRAFAAAGVCPAPDGHEPFTRGTWV
RSGQHAITRRKFWLAYQSDPAGSVHVDDGAAKALLHKGSSLLPGGVCAVEGTFQRGGLVRVLHKGESIGVGLSNYSAPEL
KKIMGLKRHEVAAILGDAHYPEVIHRDNLLVDAAV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=288, Percent_Identity=32.2916666666667, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=33.2167832167832, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786437, Length=397, Percent_Identity=37.0277078085642, Blast_Score=229, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17569967, Length=280, Percent_Identity=33.9285714285714, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17569969, Length=280, Percent_Identity=33.9285714285714, Blast_Score=118, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6320506, Length=431, Percent_Identity=32.0185614849188, Blast_Score=183, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6321822, Length=431, Percent_Identity=29.6983758700696, Blast_Score=180, Evalue=4e-46,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=32.3024054982818, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 46063; Mature: 45932

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQRPHGTASGAGRPEQCGTCGSEEERSETWQQERVRVLAQARVIVVKVGSAVLTDASG
CCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHEECCCC
LSSPVLAGLAAQLAALRDLPLVSDPAGQPQQGARRLVLVSSGAVAAGRAALTSRGRVVET
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCEEEEEECCCCHHCHHHHHCCCCEEEH
AGLAARQAAAAVGQGQLMQAWDKVFLAHGMPTAQVLLTRDDLRARQRFLNARNTFAELLH
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHH
WDVLPIVNENDTVSISELKFGDNDCLASLLINLVEADLFINLTSSSGVLAADPQKYPDAP
HCCEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCCCEEEECCCCCCCCC
VMDHIDDVATLDLGQLCGGKTLVGTGGMYSKLLAARRAAQLGVPTLILPGREPWVISRAF
HHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEHHH
AAAGVCPAPDGHEPFTRGTWVRSGQHAITRRKFWLAYQSDPAGSVHVDDGAAKALLHKGS
HHCCCCCCCCCCCCCCCCCEEECCCHHHHEEEEEEEEECCCCCEEEECCHHHHHHHHCCC
SLLPGGVCAVEGTFQRGGLVRVLHKGESIGVGLSNYSAPELKKIMGLKRHEVAAILGDAH
CCCCCCEEEEECCCCCCCEEEEEECCCCEEECCCCCCCHHHHHHHCCCHHHHHHHHCCCC
YPEVIHRDNLLVDAAV
CCHHHCCCCEEEEECC
>Mature Secondary Structure 
SRQRPHGTASGAGRPEQCGTCGSEEERSETWQQERVRVLAQARVIVVKVGSAVLTDASG
CCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHEECCCC
LSSPVLAGLAAQLAALRDLPLVSDPAGQPQQGARRLVLVSSGAVAAGRAALTSRGRVVET
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCEEEEEECCCCHHCHHHHHCCCCEEEH
AGLAARQAAAAVGQGQLMQAWDKVFLAHGMPTAQVLLTRDDLRARQRFLNARNTFAELLH
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHH
WDVLPIVNENDTVSISELKFGDNDCLASLLINLVEADLFINLTSSSGVLAADPQKYPDAP
HCCEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCCCEEEECCCCCCCCC
VMDHIDDVATLDLGQLCGGKTLVGTGGMYSKLLAARRAAQLGVPTLILPGREPWVISRAF
HHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEHHH
AAAGVCPAPDGHEPFTRGTWVRSGQHAITRRKFWLAYQSDPAGSVHVDDGAAKALLHKGS
HHCCCCCCCCCCCCCCCCCEEECCCHHHHEEEEEEEEECCCCCEEEECCHHHHHHHHCCC
SLLPGGVCAVEGTFQRGGLVRVLHKGESIGVGLSNYSAPELKKIMGLKRHEVAAILGDAH
CCCCCCEEEEECCCCCCCEEEEEECCCCEEECCCCCCCHHHHHHHCCCHHHHHHHHCCCC
YPEVIHRDNLLVDAAV
CCHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA