Click here to switch to the map view.

The map label for this gene is cobD [H]

Identifier: 220905402

GI number: 220905402

Start: 2579508

End: 2580662

Strand: Reverse

Name: cobD [H]

Synonym: Ddes_2140

Alternate gene names: 220905402

Gene position: 2580662-2579508 (Counterclockwise)

Preceding gene: 220905403

Following gene: 220905401

Centisome position: 89.81

GC content: 66.58

Gene sequence:

>1155_bases
GTGAGCCTGGGTCACGGGTTTTCCCTGGTATTTCCCTGGTCTGTGTGGGAATGCTGGTGGCTGGCCCCTCTGGCCCTTGT
TCTGGATTTGTGGCTTGGCGATCCTGCTTTGCCCTGGCGGCATCCTGTCTGCATTGTGGGCAGGTTTCTGGACGCTCTGG
AAGGCCCTGCGCGGTGCTTTATGGCGACCGGCGCGGCAGAAAAACAGCGTTTGCGCGGCCGCCTTGCCGGGGCGGCGGCC
CTGGCCGTGCTGACGTTCTGTACGGGACTGGCCGCCTGGAGCCTGACATCGCTGCCGTTGTTGGCCCTGCCGCTGGCGGT
ATACCTGGGCTGGTCCGGCCTTGCCATGGGCAGCCTGCTGCAAACCGGGCGCACTGTGCTGGAACGTGTGGAACATGCGC
CCGTGCCTGAAGCCCGTGAGGCACTCTCCTGGCTCGTCAGTCGCGAGACCGGCAGCATGGATCGACCGCTCATGCGCAAG
ACGCTGGCCGATACCCTTTCTGAAAATCTCACAGACGCTTTTACCGCGCCTTTTTTCTGGCTGCTGGTTGGCGGCCCTGT
AGCCCTGTGGTGCTATAAGGCCGTCAGCACCACGGATTCCATGTGGGGCTACACCACTGACAAGTGGCGCTGGCTCGGCT
GGGCCGGAGCGCGGGCTGACGACGCCCTGGCCTTTATTCCGGCCCGCCTGGCTGCGCTGAGTACGGCGCTGGCCCATGTG
CTGGCCTGTCTGTGCGCGCGGCTTGCCGGCGCTTTCGGCCGCCGGTGCGTGCGGGTAGCGGCCCGCCAGCCTTTGCAGCG
CCTCATCCGTTGCGCGGCCAGGCTTCCGGCATGGCCGGGGCGCTGGCCCGGCCTTGGCGTGGTGGCCCGTCAGGCCGTGG
GCATGCCCAGTCCCAATTCCGGCTGGTCCATGACGGCCTGCGCCTGGCTGTGCTGGGCGCGCATGGCAGGGCCGTCGGTC
TATTTTGGAGTGCTGACGCCCAAGCCCTGGCTTGGACCGTCGCCAGAGGATGAGGAACGTCGCGGCCTTTCGGCAGCATG
GGATGTTTCCCGCCTGTCGGCCCTGTGCTCCCTGCTGTGGTGGAGCGCCCTGTGTGGTGGCCTGGCCCTCTGGCTTACGG
CTCTTTTGCTGCTTTCTGGCACAGTGTGGGCGTGA

Upstream 100 bases:

>100_bases
CCATGCTGGAAGGGCGCGTGCGCAGCATCAACCTGTCCACAGCGGCGGGTATTGTGCTTTATGCGGCCCTGGCATGCACT
GGCCTTATGGAGGACTGGGC

Downstream 100 bases:

>100_bases
CAGTACCCTGTGCGATATTGCCCGGGCGACTGCGTGATGCTTTTCCCGCCAGAAGCGCACGCGTGATGCATATGCGCGGC
TGAACGGCGGAGCTGGTGCC

Product: cobalamin biosynthesis protein CbiB

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSLGHGFSLVFPWSVWECWWLAPLALVLDLWLGDPALPWRHPVCIVGRFLDALEGPARCFMATGAAEKQRLRGRLAGAAA
LAVLTFCTGLAAWSLTSLPLLALPLAVYLGWSGLAMGSLLQTGRTVLERVEHAPVPEAREALSWLVSRETGSMDRPLMRK
TLADTLSENLTDAFTAPFFWLLVGGPVALWCYKAVSTTDSMWGYTTDKWRWLGWAGARADDALAFIPARLAALSTALAHV
LACLCARLAGAFGRRCVRVAARQPLQRLIRCAARLPAWPGRWPGLGVVARQAVGMPSPNSGWSMTACAWLCWARMAGPSV
YFGVLTPKPWLGPSPEDEERRGLSAAWDVSRLSALCSLLWWSALCGGLALWLTALLLLSGTVWA

Sequences:

>Translated_384_residues
MSLGHGFSLVFPWSVWECWWLAPLALVLDLWLGDPALPWRHPVCIVGRFLDALEGPARCFMATGAAEKQRLRGRLAGAAA
LAVLTFCTGLAAWSLTSLPLLALPLAVYLGWSGLAMGSLLQTGRTVLERVEHAPVPEAREALSWLVSRETGSMDRPLMRK
TLADTLSENLTDAFTAPFFWLLVGGPVALWCYKAVSTTDSMWGYTTDKWRWLGWAGARADDALAFIPARLAALSTALAHV
LACLCARLAGAFGRRCVRVAARQPLQRLIRCAARLPAWPGRWPGLGVVARQAVGMPSPNSGWSMTACAWLCWARMAGPSV
YFGVLTPKPWLGPSPEDEERRGLSAAWDVSRLSALCSLLWWSALCGGLALWLTALLLLSGTVWA
>Mature_383_residues
SLGHGFSLVFPWSVWECWWLAPLALVLDLWLGDPALPWRHPVCIVGRFLDALEGPARCFMATGAAEKQRLRGRLAGAAAL
AVLTFCTGLAAWSLTSLPLLALPLAVYLGWSGLAMGSLLQTGRTVLERVEHAPVPEAREALSWLVSRETGSMDRPLMRKT
LADTLSENLTDAFTAPFFWLLVGGPVALWCYKAVSTTDSMWGYTTDKWRWLGWAGARADDALAFIPARLAALSTALAHVL
ACLCARLAGAFGRRCVRVAARQPLQRLIRCAARLPAWPGRWPGLGVVARQAVGMPSPNSGWSMTACAWLCWARMAGPSVY
FGVLTPKPWLGPSPEDEERRGLSAAWDVSRLSALCSLLWWSALCGGLALWLTALLLLSGTVWA

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 41764; Mature: 41633

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGHGFSLVFPWSVWECWWLAPLALVLDLWLGDPALPWRHPVCIVGRFLDALEGPARCF
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
MATGAAEKQRLRGRLAGAAALAVLTFCTGLAAWSLTSLPLLALPLAVYLGWSGLAMGSLL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QTGRTVLERVEHAPVPEAREALSWLVSRETGSMDRPLMRKTLADTLSENLTDAFTAPFFW
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVGGPVALWCYKAVSTTDSMWGYTTDKWRWLGWAGARADDALAFIPARLAALSTALAHV
HHHCCHHHHHHHHHHHCCCHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LACLCARLAGAFGRRCVRVAARQPLQRLIRCAARLPAWPGRWPGLGVVARQAVGMPSPNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCC
GWSMTACAWLCWARMAGPSVYFGVLTPKPWLGPSPEDEERRGLSAAWDVSRLSALCSLLW
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHH
WSALCGGLALWLTALLLLSGTVWA
HHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SLGHGFSLVFPWSVWECWWLAPLALVLDLWLGDPALPWRHPVCIVGRFLDALEGPARCF
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
MATGAAEKQRLRGRLAGAAALAVLTFCTGLAAWSLTSLPLLALPLAVYLGWSGLAMGSLL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QTGRTVLERVEHAPVPEAREALSWLVSRETGSMDRPLMRKTLADTLSENLTDAFTAPFFW
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVGGPVALWCYKAVSTTDSMWGYTTDKWRWLGWAGARADDALAFIPARLAALSTALAHV
HHHCCHHHHHHHHHHHCCCHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LACLCARLAGAFGRRCVRVAARQPLQRLIRCAARLPAWPGRWPGLGVVARQAVGMPSPNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCC
GWSMTACAWLCWARMAGPSVYFGVLTPKPWLGPSPEDEERRGLSAAWDVSRLSALCSLLW
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHH
WSALCGGLALWLTALLLLSGTVWA
HHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA