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The map label for this gene is trmL [H]

Identifier: 220905403

GI number: 220905403

Start: 2580659

End: 2581141

Strand: Reverse

Name: trmL [H]

Synonym: Ddes_2141

Alternate gene names: 220905403

Gene position: 2581141-2580659 (Counterclockwise)

Preceding gene: 220905404

Following gene: 220905402

Centisome position: 89.83

GC content: 60.87

Gene sequence:

>483_bases
ATGCAGATAGTTCTTTACGAGCCGGAAATACCCCCCAATACGGGCAATGTGGCCCGCTTGTGCGCAGCCACGGGCGTAAC
GCTCAATCTTATAGAGCCGCTGGGCTTCAAGCTTGAAGACCGCTACCTCAAACGCGCGGGGCTGGACTACTGGCCCAATG
TGCGCTTGCAGGTCTGGCCCTGCTGGAGTGACTTTACGCGGGAGGCTCTGGCGGATGGGCGCGCGGGGCGGCTGGTATTC
ACCTCGGCAAAGAGCGCCGGGGGCAGTGTGCCGGTACACCATTTTGCCTTTGATCCTGATGACTTTCTGGTTTTCGGTCC
GGAAACGCGCGGCCTGCCGCAGGAAATTCTGGAACTTTCGCCGCACAGGGTGCGCATTCCCATGCTGGAAGGGCGCGTGC
GCAGCATCAACCTGTCCACAGCGGCGGGTATTGTGCTTTATGCGGCCCTGGCATGCACTGGCCTTATGGAGGACTGGGCG
TGA

Upstream 100 bases:

>100_bases
GTCCGGGCAAAAACAGTGCGGAAACCGCCGAGGTTTTCGCCCGCCGGAAGCGCCGCCGTATTTCGGCGGCCTGCGGCATA
TGCGCCGAAATGGAAAAAGC

Downstream 100 bases:

>100_bases
GCCTGGGTCACGGGTTTTCCCTGGTATTTCCCTGGTCTGTGTGGGAATGCTGGTGGCTGGCCCCTCTGGCCCTTGTTCTG
GATTTGTGGCTTGGCGATCC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MQIVLYEPEIPPNTGNVARLCAATGVTLNLIEPLGFKLEDRYLKRAGLDYWPNVRLQVWPCWSDFTREALADGRAGRLVF
TSAKSAGGSVPVHHFAFDPDDFLVFGPETRGLPQEILELSPHRVRIPMLEGRVRSINLSTAAGIVLYAALACTGLMEDWA

Sequences:

>Translated_160_residues
MQIVLYEPEIPPNTGNVARLCAATGVTLNLIEPLGFKLEDRYLKRAGLDYWPNVRLQVWPCWSDFTREALADGRAGRLVF
TSAKSAGGSVPVHHFAFDPDDFLVFGPETRGLPQEILELSPHRVRIPMLEGRVRSINLSTAAGIVLYAALACTGLMEDWA
>Mature_160_residues
MQIVLYEPEIPPNTGNVARLCAATGVTLNLIEPLGFKLEDRYLKRAGLDYWPNVRLQVWPCWSDFTREALADGRAGRLVF
TSAKSAGGSVPVHHFAFDPDDFLVFGPETRGLPQEILELSPHRVRIPMLEGRVRSINLSTAAGIVLYAALACTGLMEDWA

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=152, Percent_Identity=44.7368421052632, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17694; Mature: 17694

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIVLYEPEIPPNTGNVARLCAATGVTLNLIEPLGFKLEDRYLKRAGLDYWPNVRLQVWP
CEEEEECCCCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEE
CWSDFTREALADGRAGRLVFTSAKSAGGSVPVHHFAFDPDDFLVFGPETRGLPQEILELS
CHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHCC
PHRVRIPMLEGRVRSINLSTAAGIVLYAALACTGLMEDWA
CCEEEEEEECCEEEEEECHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIVLYEPEIPPNTGNVARLCAATGVTLNLIEPLGFKLEDRYLKRAGLDYWPNVRLQVWP
CEEEEECCCCCCCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEE
CWSDFTREALADGRAGRLVFTSAKSAGGSVPVHHFAFDPDDFLVFGPETRGLPQEILELS
CHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHCC
PHRVRIPMLEGRVRSINLSTAAGIVLYAALACTGLMEDWA
CCEEEEEEECCEEEEEECHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA