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The map label for this gene is crp [H]

Identifier: 220905354

GI number: 220905354

Start: 2520029

End: 2520700

Strand: Reverse

Name: crp [H]

Synonym: Ddes_2092

Alternate gene names: 220905354

Gene position: 2520700-2520029 (Counterclockwise)

Preceding gene: 220905370

Following gene: 220905350

Centisome position: 87.72

GC content: 52.98

Gene sequence:

>672_bases
ATGGTTGCCGAGGGTGTCAAGGGCAGTCGCAACTGGGCCTTTAAAGAAATGAAGGGTATGAACGCCCCCTGGCGGTCGGT
ACTGTATCTTGCCAGACGTTTTGCCTGGGCAAAGGGACATGAGGTGCAGTTTCGCAACCGTCTTTATTTTTTAGAGAAAG
GTCGCGTCAGACTGACGCATCAAAGCCTTGAAGGTATGGAGAAAATTCTCTGGTACATTCACGAGGGCTGCATTTTCGGC
GAAACGCCCTTTCTGGATCCCATGCCTTTGGAGAGTTTTTTCACGTGTGCCACGGATTGCGTGGTTTACGGTTTTTCAAA
AGAGAGCTTTGATGTGATCAGCCGGGAGCGGCCTGACCTTGTGTCCAACCTGCTGCTCTCCATGTCGCGCAAGCTGCGGA
TACTGTCCAACCAGGCTTCTTCCCTGTACGTGGATAACCTGCTCGTGCGGGTCTGCAAGTTTATGGCGCAGCATCTTGAG
CCGGACAGTCACCCCCTTACGGCGAACCTGGGTATTTCGCGGCAGGAGATGGCAAGCCTGCTTGGGGTACACCGCGTTTC
TTTGTACAAGGTACTGAAGCAGCAGGAAGAGGAAGGGCTTTTTGGTCCCTTTGAGGGCAATGCGGTGGTGATTTTGCAGC
CCGAGGAGTTTTTTAGGCTGGCTTCTGCATAA

Upstream 100 bases:

>100_bases
GGCGTGTTCCGTATGCAACATTTTTCACAATAAATTTTTCGGGATGGCGCGGGCTGTATGAAGACCGCTGCTTTTTGGGG
GTAACTTCATGGAGAAACAG

Downstream 100 bases:

>100_bases
TGCACGTCCGGTACACGGAAAGGGGCGCGCTGCCGTAGCACGGCTGCGCGCCCCTTTGGGCTTCAGATAAAGGAATATCC
TGTTTTTCAGGGCACGGGCG

Product: cyclic nucleotide-binding protein

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MVAEGVKGSRNWAFKEMKGMNAPWRSVLYLARRFAWAKGHEVQFRNRLYFLEKGRVRLTHQSLEGMEKILWYIHEGCIFG
ETPFLDPMPLESFFTCATDCVVYGFSKESFDVISRERPDLVSNLLLSMSRKLRILSNQASSLYVDNLLVRVCKFMAQHLE
PDSHPLTANLGISRQEMASLLGVHRVSLYKVLKQQEEEGLFGPFEGNAVVILQPEEFFRLASA

Sequences:

>Translated_223_residues
MVAEGVKGSRNWAFKEMKGMNAPWRSVLYLARRFAWAKGHEVQFRNRLYFLEKGRVRLTHQSLEGMEKILWYIHEGCIFG
ETPFLDPMPLESFFTCATDCVVYGFSKESFDVISRERPDLVSNLLLSMSRKLRILSNQASSLYVDNLLVRVCKFMAQHLE
PDSHPLTANLGISRQEMASLLGVHRVSLYKVLKQQEEEGLFGPFEGNAVVILQPEEFFRLASA
>Mature_223_residues
MVAEGVKGSRNWAFKEMKGMNAPWRSVLYLARRFAWAKGHEVQFRNRLYFLEKGRVRLTHQSLEGMEKILWYIHEGCIFG
ETPFLDPMPLESFFTCATDCVVYGFSKESFDVISRERPDLVSNLLLSMSRKLRILSNQASSLYVDNLLVRVCKFMAQHLE
PDSHPLTANLGISRQEMASLLGVHRVSLYKVLKQQEEEGLFGPFEGNAVVILQPEEFFRLASA

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=176, Percent_Identity=25.5681818181818, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25612; Mature: 25612

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAEGVKGSRNWAFKEMKGMNAPWRSVLYLARRFAWAKGHEVQFRNRLYFLEKGRVRLTH
CCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEHHCEEEEEECCCEEEHH
QSLEGMEKILWYIHEGCIFGETPFLDPMPLESFFTCATDCVVYGFSKESFDVISRERPDL
HHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHCCCHHH
VSNLLLSMSRKLRILSNQASSLYVDNLLVRVCKFMAQHLEPDSHPLTANLGISRQEMASL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHH
LGVHRVSLYKVLKQQEEEGLFGPFEGNAVVILQPEEFFRLASA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHCCC
>Mature Secondary Structure
MVAEGVKGSRNWAFKEMKGMNAPWRSVLYLARRFAWAKGHEVQFRNRLYFLEKGRVRLTH
CCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEHHCEEEEEECCCEEEHH
QSLEGMEKILWYIHEGCIFGETPFLDPMPLESFFTCATDCVVYGFSKESFDVISRERPDL
HHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHCCCHHH
VSNLLLSMSRKLRILSNQASSLYVDNLLVRVCKFMAQHLEPDSHPLTANLGISRQEMASL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHH
LGVHRVSLYKVLKQQEEEGLFGPFEGNAVVILQPEEFFRLASA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]