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The map label for this gene is yfkN [H]

Identifier: 220904779

GI number: 220904779

Start: 1803664

End: 1805250

Strand: Reverse

Name: yfkN [H]

Synonym: Ddes_1511

Alternate gene names: 220904779

Gene position: 1805250-1803664 (Counterclockwise)

Preceding gene: 220904780

Following gene: 220904773

Centisome position: 62.83

GC content: 57.15

Gene sequence:

>1587_bases
ATGTTTTTTGCAAGACCCCTGTTTTTTCTTTTTCTGCTGTTTTGTACTGCGGTTCCGGCCCTGGCGGACATAAAAATATT
TATCACCAATGATGTTCATGGCCACGTAGCTGACAATCCGGAAAAAAAGCGCATCGGCTACGCGCGGCTCAAGGCATTGG
CCGATGATGCGCGGGCCGAAGGGCATACCGTCTTTATTCTGGATGCGGGAGATGCCTTCAGCGGCAGCGCCTATGCGCAG
AAAGACAAGGGCCGCTCAGTGGCAAAGCTTATGGGACAGGTGGGCTACCGCGTACTTACGCCGGGAAATCACGCCTTTGA
CTATGCCGCATCCGAAGGCCCCCTGCACTACAGCAATGAACTGCTGCGCCTCGTGCGCGAAAACAGCCCGGGCAAGGTTG
ATGCCGTGGCCGAAAACCTCACATACAAGGGGGCGAATACGCCGGGCATGCTCACAGAGCCTGTGGTCATCCATGACGAA
ACATCAGAAAATGAACACGGATTGCGCGTTATTGTCACTGGCGTCATCACGCCATATGCGGTGAAACCCGGCATGCGTCA
TGTGCTTGAAGATTATGATTTTGACCTTAAACCTACGGCAGAAGACACCAAAAAAGCCATTCTGGAGCGGCTGAAGCATT
CGCTTGCGCCCTATGACCGGGCGGAAGATATCGTCATCGTGCTGAGCCACCTTGGGCATGCGGGACCACAGGGTGATAGG
GACGGCCGCATTACAGGGCCTGATGTGGCCGCCGTACCGCACGTGGATTTTGTGGCGGACGGGCACAGTCATCAGGCCGT
GGCCCCGAGCCGCCAATACGGGGCAATATATGCCAACGGCGGGCGTTATCTGGAGCACGTCATGGAGATCACCCTTGACG
GCAAGGGGGGTGACATGGCGCTGAAGTCATATGATGATGTGGCGGACGTCGTGCCGGACAGGGGCATGGCGGAGCGTATA
CGCCAGCTTGATGCGGCCTGGGGCTTTGCAGAAGTGCTTTTTACTTCTCCCGATGACAGTGTTTTCAGTGACCGGCGATT
GAGGAAGGACAGCACACCTTTGGGTCGCCTTATTTGCGAGGGCATGGCAAAGGCCACCGGTTCTGCCGTTGCCCTGCACA
ATCCCGGCGGCATACGCGCGGGATTGCCCGGCGGACCTGTACGCCTGCGCGACCTGCACGATGTTTTGCCTTTTGGCGAC
ACCCTGGTTACAGCCGATATGACAGGCAGGCAAATTGCCGGAATACTCATGCGCGGCGCAGGGCATGGCGGGCGTGGCTT
GCCGCAGTTTTACGGGCTCACTGCCTGGGCCTGGCGGGATGGAGAGGGCACTCTGCATGTGGCCGGCTTTCGTCTCGCAG
ACGGAGAGCCATTGCAGCCGGACAGGACGTACCGTGTGGCTCTTAACGGTTTTATGGCCAGAAATATGGATCTGCCCACG
AAAAAAGACCACGGAACATTGGTGGATGTTTTGGAAAAACAGCTAAAAAAAGGTGTTGACGCCGAAGCCTTGCGGTCAGG
CCAGAATCTGTATGTTTTTGCTGACAGATCTGCGGCGGAGGCGGCCTGGCAGGCCGGAATCCGCTGA

Upstream 100 bases:

>100_bases
GCCCTTGAGGCGGTGCGCAGCGCAGCGCCTTTTGCAGCGCCACCGGAACCGGCGCGAGTGATCATGCCCGTGCAGTTCAG
CCTGAGGTAGTGAGGTGATC

Downstream 100 bases:

>100_bases
CGACGGTCTTCAGGCACGGGGGAGAACAACTTAATATTTACAACGTCTTATATCTGGTCCGTCTGGTGACGGATGGCAGA
CGCCCTGTCAGGGGCTGCCG

Product: 5'-Nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MFFARPLFFLFLLFCTAVPALADIKIFITNDVHGHVADNPEKKRIGYARLKALADDARAEGHTVFILDAGDAFSGSAYAQ
KDKGRSVAKLMGQVGYRVLTPGNHAFDYAASEGPLHYSNELLRLVRENSPGKVDAVAENLTYKGANTPGMLTEPVVIHDE
TSENEHGLRVIVTGVITPYAVKPGMRHVLEDYDFDLKPTAEDTKKAILERLKHSLAPYDRAEDIVIVLSHLGHAGPQGDR
DGRITGPDVAAVPHVDFVADGHSHQAVAPSRQYGAIYANGGRYLEHVMEITLDGKGGDMALKSYDDVADVVPDRGMAERI
RQLDAAWGFAEVLFTSPDDSVFSDRRLRKDSTPLGRLICEGMAKATGSAVALHNPGGIRAGLPGGPVRLRDLHDVLPFGD
TLVTADMTGRQIAGILMRGAGHGGRGLPQFYGLTAWAWRDGEGTLHVAGFRLADGEPLQPDRTYRVALNGFMARNMDLPT
KKDHGTLVDVLEKQLKKGVDAEALRSGQNLYVFADRSAAEAAWQAGIR

Sequences:

>Translated_528_residues
MFFARPLFFLFLLFCTAVPALADIKIFITNDVHGHVADNPEKKRIGYARLKALADDARAEGHTVFILDAGDAFSGSAYAQ
KDKGRSVAKLMGQVGYRVLTPGNHAFDYAASEGPLHYSNELLRLVRENSPGKVDAVAENLTYKGANTPGMLTEPVVIHDE
TSENEHGLRVIVTGVITPYAVKPGMRHVLEDYDFDLKPTAEDTKKAILERLKHSLAPYDRAEDIVIVLSHLGHAGPQGDR
DGRITGPDVAAVPHVDFVADGHSHQAVAPSRQYGAIYANGGRYLEHVMEITLDGKGGDMALKSYDDVADVVPDRGMAERI
RQLDAAWGFAEVLFTSPDDSVFSDRRLRKDSTPLGRLICEGMAKATGSAVALHNPGGIRAGLPGGPVRLRDLHDVLPFGD
TLVTADMTGRQIAGILMRGAGHGGRGLPQFYGLTAWAWRDGEGTLHVAGFRLADGEPLQPDRTYRVALNGFMARNMDLPT
KKDHGTLVDVLEKQLKKGVDAEALRSGQNLYVFADRSAAEAAWQAGIR
>Mature_528_residues
MFFARPLFFLFLLFCTAVPALADIKIFITNDVHGHVADNPEKKRIGYARLKALADDARAEGHTVFILDAGDAFSGSAYAQ
KDKGRSVAKLMGQVGYRVLTPGNHAFDYAASEGPLHYSNELLRLVRENSPGKVDAVAENLTYKGANTPGMLTEPVVIHDE
TSENEHGLRVIVTGVITPYAVKPGMRHVLEDYDFDLKPTAEDTKKAILERLKHSLAPYDRAEDIVIVLSHLGHAGPQGDR
DGRITGPDVAAVPHVDFVADGHSHQAVAPSRQYGAIYANGGRYLEHVMEITLDGKGGDMALKSYDDVADVVPDRGMAERI
RQLDAAWGFAEVLFTSPDDSVFSDRRLRKDSTPLGRLICEGMAKATGSAVALHNPGGIRAGLPGGPVRLRDLHDVLPFGD
TLVTADMTGRQIAGILMRGAGHGGRGLPQFYGLTAWAWRDGEGTLHVAGFRLADGEPLQPDRTYRVALNGFMARNMDLPT
KKDHGTLVDVLEKQLKKGVDAEALRSGQNLYVFADRSAAEAAWQAGIR

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1786687, Length=504, Percent_Identity=25.1984126984127, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 57273; Mature: 57273

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFARPLFFLFLLFCTAVPALADIKIFITNDVHGHVADNPEKKRIGYARLKALADDARAE
CCCCCHHHHHHHHHHHHCCCHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GHTVFILDAGDAFSGSAYAQKDKGRSVAKLMGQVGYRVLTPGNHAFDYAASEGPLHYSNE
CCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHCCCEEECCCCCCEECCCCCCCCHHHHH
LLRLVRENSPGKVDAVAENLTYKGANTPGMLTEPVVIHDETSENEHGLRVIVTGVITPYA
HHHHHHCCCCCCCHHHHHCCEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCHH
VKPGMRHVLEDYDFDLKPTAEDTKKAILERLKHSLAPYDRAEDIVIVLSHLGHAGPQGDR
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHEEHHHCCCCCCCCC
DGRITGPDVAAVPHVDFVADGHSHQAVAPSRQYGAIYANGGRYLEHVMEITLDGKGGDMA
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHEEECCCCCCEE
LKSYDDVADVVPDRGMAERIRQLDAAWGFAEVLFTSPDDSVFSDRRLRKDSTPLGRLICE
HHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCHHHHHHHH
GMAKATGSAVALHNPGGIRAGLPGGPVRLRDLHDVLPFGDTLVTADMTGRQIAGILMRGA
HHHHCCCCEEEEECCCCCCCCCCCCCEEHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCC
GHGGRGLPQFYGLTAWAWRDGEGTLHVAGFRLADGEPLQPDRTYRVALNGFMARNMDLPT
CCCCCCCHHHHCCEEEEEECCCCEEEEEEEEECCCCCCCCCCEEEEEEHHHHHCCCCCCC
KKDHGTLVDVLEKQLKKGVDAEALRSGQNLYVFADRSAAEAAWQAGIR
CCCCCHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCHHHHHHHCCCC
>Mature Secondary Structure
MFFARPLFFLFLLFCTAVPALADIKIFITNDVHGHVADNPEKKRIGYARLKALADDARAE
CCCCCHHHHHHHHHHHHCCCHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
GHTVFILDAGDAFSGSAYAQKDKGRSVAKLMGQVGYRVLTPGNHAFDYAASEGPLHYSNE
CCEEEEEECCCCCCCCCCCCHHCCHHHHHHHHHCCCEEECCCCCCEECCCCCCCCHHHHH
LLRLVRENSPGKVDAVAENLTYKGANTPGMLTEPVVIHDETSENEHGLRVIVTGVITPYA
HHHHHHCCCCCCCHHHHHCCEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCHH
VKPGMRHVLEDYDFDLKPTAEDTKKAILERLKHSLAPYDRAEDIVIVLSHLGHAGPQGDR
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHEEHHHCCCCCCCCC
DGRITGPDVAAVPHVDFVADGHSHQAVAPSRQYGAIYANGGRYLEHVMEITLDGKGGDMA
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHEEECCCCCCEE
LKSYDDVADVVPDRGMAERIRQLDAAWGFAEVLFTSPDDSVFSDRRLRKDSTPLGRLICE
HHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCHHHHHHHH
GMAKATGSAVALHNPGGIRAGLPGGPVRLRDLHDVLPFGDTLVTADMTGRQIAGILMRGA
HHHHCCCCEEEEECCCCCCCCCCCCCEEHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCC
GHGGRGLPQFYGLTAWAWRDGEGTLHVAGFRLADGEPLQPDRTYRVALNGFMARNMDLPT
CCCCCCCHHHHCCEEEEEECCCCEEEEEEEEECCCCCCCCCCEEEEEEHHHHHCCCCCCC
KKDHGTLVDVLEKQLKKGVDAEALRSGQNLYVFADRSAAEAAWQAGIR
CCCCCHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]