Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 220904706

GI number: 220904706

Start: 1722882

End: 1724258

Strand: Direct

Name: shiA [H]

Synonym: Ddes_1438

Alternate gene names: 220904706

Gene position: 1722882-1724258 (Clockwise)

Preceding gene: 220904705

Following gene: 220904711

Centisome position: 59.96

GC content: 54.68

Gene sequence:

>1377_bases
ATGAGTGTTGCCGCCAATGACAAGCAGGCGCAAAAAAACCTGCGCCGCGTGGTGATTTCCTCCCTTATGGGCGCGGTTAT
CGAATGGTATGACTTTTTCCTTTATGGTGTGGTGGCCGGGCTTGTGTTCAACAAGCTTTATTTTCCCGCATTCTCACCGG
GCGTAGGCACTATTCTGGCATTCGCCACCTTTGCTGTGGGCTTTGTGGCCCGGCCTATGGGCGGCATCATTTTTGGTCAC
TTCGGCGACAAGATCGGCCGTAAAAAAATGCTCATCCTCACCCTTGAGATTATGGGCGTGGCCACGGTGCTCATCGGCTG
CATTCCCTCTTACGACAGCATCGGCATATGGGCTCCCATCCTGCTGGTTATCTGCCGTCTGGCCCAGGGGATCGGGCTTG
GCGGCGAATGGGGAGGCGCCGTGCTTATGGCTTATGAATCCGCACCAGCCCACAAGCGCGCTTTTTATGGCAGCTTGCCC
CAGGTTGGCCTTTCACTGGGCCTGATGCTCGCTTCAGGGGTTATCGGTCTGCTGTCATTCCTGCTGTCTGACGAGGCCTT
TCTTGCCTGGGGCTGGCGTATTGCCTTTATCCTGAGCGCAGTTCTGGTACTGGTGGGCGCGTACATACGCACGTCCGTTC
AGGAAACACAAGACTTTTCTTCCGCTAAAAAAGAAGTGGAAAAAATCCGCTATCCCATGCTTGAAGCGTTCAAGCGCTAT
CCCAAAACCCTGACCGCCTGCGTGGGAGCGCGCTTTGTAGAAGGTATCGCCTTTAACGTGTTTGGTGTGTTTTCATTGAC
GTACCTTACACAGACCTGCGGCGTTAACCGCACTGTGGCCCTTATGGCCGTTGTGGTGGCCTCGGGTGTTATGGCCTGCT
TCATTCCCATGTGGGGAGCCATGGCAGACCGCGTGGGCAAAGGCCGCATTTTCGGCACTGCGGCACTGCTGCTGGGCATA
ACCGCTTATCCGGTGTTCTGGGTGCTGCACAACTATGCCACCACGAATCTCTTCTTTGTCTATCTGGCGATTATCATCCC
CTTCGGTATTATCTATGCCGCGGCCTATGCCAGCATGGCCAGCCTGTTTTCTGAAAGTTTTGATGCCACGGTGCGGTATT
CCAGCATTTCTTTTGTATATCAGTTTTCGGGCATTTTTGCTTCGGGGCTTACCCCCATGATCGCGACCATGCTCGTGCAG
GCCAACAATTCTCAACCCTGGTATCTCTGCGGATATCTGCTTGTGGCAGGTGTTATCAGCACCGTGTCCACCATGTGGAT
AGGCAGACTCCGGATTTCAAAGGCTGAGCCGCGCGTATTGCCCGCTGAAGAAAGCAACGTTCTTGCCGCCAATCCTGCAA
CCGGTCAGAACGCCTGA

Upstream 100 bases:

>100_bases
TGGAAGCCTACCGCGAGTTTGACCTGACTGATGAGGCCCGCCGCAAAATCATGTATGAAAACGCCCGCCGCGTGCTGGGC
CTGGCATAAAGGAGAACGGT

Downstream 100 bases:

>100_bases
CACCTCCAGAGTCTGCCCTGAAAAAGACCGGGGATTGCCGACCCCGGCCCTGAAGAATCAGGCCGCAACCCTGCGGATGC
AGACGCCAAGCCCCCCCTGT

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSVAANDKQAQKNLRRVVISSLMGAVIEWYDFFLYGVVAGLVFNKLYFPAFSPGVGTILAFATFAVGFVARPMGGIIFGH
FGDKIGRKKMLILTLEIMGVATVLIGCIPSYDSIGIWAPILLVICRLAQGIGLGGEWGGAVLMAYESAPAHKRAFYGSLP
QVGLSLGLMLASGVIGLLSFLLSDEAFLAWGWRIAFILSAVLVLVGAYIRTSVQETQDFSSAKKEVEKIRYPMLEAFKRY
PKTLTACVGARFVEGIAFNVFGVFSLTYLTQTCGVNRTVALMAVVVASGVMACFIPMWGAMADRVGKGRIFGTAALLLGI
TAYPVFWVLHNYATTNLFFVYLAIIIPFGIIYAAAYASMASLFSESFDATVRYSSISFVYQFSGIFASGLTPMIATMLVQ
ANNSQPWYLCGYLLVAGVISTVSTMWIGRLRISKAEPRVLPAEESNVLAANPATGQNA

Sequences:

>Translated_458_residues
MSVAANDKQAQKNLRRVVISSLMGAVIEWYDFFLYGVVAGLVFNKLYFPAFSPGVGTILAFATFAVGFVARPMGGIIFGH
FGDKIGRKKMLILTLEIMGVATVLIGCIPSYDSIGIWAPILLVICRLAQGIGLGGEWGGAVLMAYESAPAHKRAFYGSLP
QVGLSLGLMLASGVIGLLSFLLSDEAFLAWGWRIAFILSAVLVLVGAYIRTSVQETQDFSSAKKEVEKIRYPMLEAFKRY
PKTLTACVGARFVEGIAFNVFGVFSLTYLTQTCGVNRTVALMAVVVASGVMACFIPMWGAMADRVGKGRIFGTAALLLGI
TAYPVFWVLHNYATTNLFFVYLAIIIPFGIIYAAAYASMASLFSESFDATVRYSSISFVYQFSGIFASGLTPMIATMLVQ
ANNSQPWYLCGYLLVAGVISTVSTMWIGRLRISKAEPRVLPAEESNVLAANPATGQNA
>Mature_457_residues
SVAANDKQAQKNLRRVVISSLMGAVIEWYDFFLYGVVAGLVFNKLYFPAFSPGVGTILAFATFAVGFVARPMGGIIFGHF
GDKIGRKKMLILTLEIMGVATVLIGCIPSYDSIGIWAPILLVICRLAQGIGLGGEWGGAVLMAYESAPAHKRAFYGSLPQ
VGLSLGLMLASGVIGLLSFLLSDEAFLAWGWRIAFILSAVLVLVGAYIRTSVQETQDFSSAKKEVEKIRYPMLEAFKRYP
KTLTACVGARFVEGIAFNVFGVFSLTYLTQTCGVNRTVALMAVVVASGVMACFIPMWGAMADRVGKGRIFGTAALLLGIT
AYPVFWVLHNYATTNLFFVYLAIIIPFGIIYAAAYASMASLFSESFDATVRYSSISFVYQFSGIFASGLTPMIATMLVQA
NNSQPWYLCGYLLVAGVISTVSTMWIGRLRISKAEPRVLPAEESNVLAANPATGQNA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=414, Percent_Identity=41.5458937198068, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1790550, Length=445, Percent_Identity=32.3595505617978, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1789941, Length=395, Percent_Identity=37.9746835443038, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1788942, Length=388, Percent_Identity=31.1855670103093, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87082404, Length=232, Percent_Identity=28.0172413793103, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082231, Length=223, Percent_Identity=23.3183856502242, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49407; Mature: 49276

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
SVAANDKQAQKNLRRVVISSLMGAVIEWYDFFLYGVVAGLVFNKLYFPAFSPGVGTILA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH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HHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]