Click here to switch to the map view.

The map label for this gene is pflD [H]

Identifier: 220904625

GI number: 220904625

Start: 1631431

End: 1633977

Strand: Direct

Name: pflD [H]

Synonym: Ddes_1357

Alternate gene names: 220904625

Gene position: 1631431-1633977 (Clockwise)

Preceding gene: 220904624

Following gene: 220904626

Centisome position: 56.78

GC content: 57.83

Gene sequence:

>2547_bases
GTGGATCTTCATGATTTTTCGAGCAAAATAGCGGAAGTTACCAAGAATCTTTCGCCTGAAGAGCGCGAGCAGTTGCGCAA
GATTTTTGCCACTGTCACCCTGCCCGCCCAGTCGGCCCCTTCCGCAGCGGCACAGCCGTCTCCCGCTGCCCACTCTGGTC
CCGGCGTACCTGAAGGGCCTACACAGCGCCATGTGCTGCTGAAAGAAAACTACCTCAAGCAGGTTCCGCGCATCACCATT
CACCGCGCCCGCACAATCACCAAGATTGACAAAGAAAACCCCGGTATGCCGCGTATTCTGCTGCGCGCCACTGCTTTTCG
CCATTGCTGCGAAAGCGCTCCGCTGATTATCCAGGATCATGAACTCATTGTCGGCGCCCCCAACGGCGCGCCGCGCGCCG
GTGCTTTTTCTCCCGAAATTTCCTGGCGCTGGCTCAGGGATGAGCTGGATACCATTGACAGTCGCGCCCAGGACCCTTTC
CACCTTGCCGAAGAAGACAAAAAGATCCTGTGTGAGGAAATCTTCCCCTACTGGGCGGGCAAGTCTGTAGATGAATACTG
TGAAGCACAGTACCGCGAAGCCGGTCTGTGGGAACTTTCGGGTGAATCCTATGTATCCGACTGCTCCTACCACGCCCTCA
ACGGCGGCGGCGACTCCAACCCCGGCTACGACGTCATTCTCATGAAGAAGGGCATGCTGGACATTCAGCGCGAAGCACGT
GAACACCTGGAACAGCTGAGCTATGACCGGCCCGAGGATATCGACAAGATCTACTTCTACAAGTCGGTTATTGAAACCAC
CGAAGGCGTCATGATCTACGCCCGGCGCATGTCCGAATACGCGGCACAGCTGGCCGCTCGCGAAAACGACCCCAGGCGCA
AGGCCGAGCTGCTCAAGATCGCCGAGGTCAACGCCCGCGTTCCCGCCCATGCCCCCACCACCTTCTGGGAAGCCATTCAG
GCCGTGTGGACCGTGGAATCACTGCTGGTTGTGGAAGAAAACCAGACAGGCATGTCCATCGGCCGTGTCGACCAGTACAT
GTACCCCTTCTACAAGGCCGATATCGAAGCGGGCCGCATGACGCCTTATGAAGCCTTTGACCTCGCAGGCTGCATGCTCA
TCAAGATGTCTGAAATGATGTGGCTCACCAGCGAAGGCAGCTCCAAATTCTTTGCCGGCTACCAGCCCTTCGTTAACATG
TGCGTTGGCGGCGTCAGCCGTGAAGGGCGCGACGCCACCAACGACCTGACCTATCTGCTCATGGATGCGGTGCGTCATGT
GCGCATCTACCAGCCCTCGCTGGCTACCCGTGTGCACAACACCTCGCCCCAGGAATACCTGAAAAAAATCGTGTCCGTCA
TCCGTTCGGGCATGGGTTTTCCGGCTGTGCACTTTGACGACACCCACATCAAGATGATGCTGGCCAAGGGCGTCGACATT
GAAGACGCCCGCGACTACTGCCTCATGGGTTGCGTGGAACCGCAAAAATCCGGCCGCCTGTATCAGTGGACGTCCACGGC
ATACACCCAGTGGCCCATCTGCATCGAGCTTGTACTCAACCGCGGCGTGCCCCTCTGGTACGGCAAGCAGGTCTGCCCTG
ACCTCGGCGATCTGAGCCAGTTCGACACTTTTGAAAAGTTTGATGCCGCCGTCAAGGAACAGATCAGGTACATCACCAAA
TGGTCAAGCGTGGCTACGGTTATCTCCCAGCGTGTCCACCGCGACCTGGCGCCCAAGCCGCTGATGTCCATCATGTACGA
AGGCTGCATGGAAAAAGGCCGCGATGTAGCCGCCGGCGGTGCCATGTACAACTTCGGTCCCGGCGTGATCTGGAGCGGCC
TTGCAACCTACGCCGACTCGATGGCCGCTATCAAGAAGCTCGTGTATGACGACAGGAAGTACACCCTGCAGCAACTTAAC
GAGGCGCTCAAAGCCAACTTTGAAGGCTATGACGCCATTCTGGCCGACTGCCTGGCCGCGCCCAAATACGGCAACGATGA
TGACTATGCCGACGATATCGCCGCCGACCTCATCGCCTTTACCGAGCGTGAGCACCGCAAGTACCGGACGCTCTACTCCA
TCCTGAGCCACGGTACACTGTCCATTTCCAACAACACGCCCTTTGGTCAGCTCCTGGGCGCTTCGGCCGGCGGCCGCAAG
GCATGGCAGCCGCTTTCCGACGGTATCAGCCCCACGCAGGGCGCGGACTACAAGGGTCCCACGGCCATCATCAAGAGCGT
GTCCAAGATGTCCAACGACAACATGAACATCGGCATGGTGCATAACTTCAAGCTCATGCCCGGCCTGCTGGATACCCCCG
AAGGCGAACAGGGCATTATCACCCTCATTCGCTCTGCCAGCATACTTGGCAACGGTGAAATGCAGTTCAACTATCTGGAC
AACAAGACCATGCTTGAAGCCCAGCAGAACCCCAAAGACTATCGCGACCTTGTGGTCCGTGTGGCAGGCTATAGCGCCTT
CTTTGTGGAACTTTGCAAGGACGTGCAGGACGAAATCATCAGCAGAACTGTGCTGCGCCACTTCTAG

Upstream 100 bases:

>100_bases
TACTGCGCGAGGCTCTGGGCGCTCTGCCCCAGGCTCGCTGACCCCGGGGCACACACCAAGCCCGCAGGCCCTTCTGACCG
CAGAATACGGAGGATAGAAC

Downstream 100 bases:

>100_bases
ACCGGATGCGGCACGTGCCGCGCCCCCTTTTTTCTCGTGGAGGGGGCGCGGCAAAATGAAAGATAACGACAGACAGCGCA
GTGCTGCGCCTACAGCAGGG

Product: Formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 848; Mature: 848

Protein sequence:

>848_residues
MDLHDFSSKIAEVTKNLSPEEREQLRKIFATVTLPAQSAPSAAAQPSPAAHSGPGVPEGPTQRHVLLKENYLKQVPRITI
HRARTITKIDKENPGMPRILLRATAFRHCCESAPLIIQDHELIVGAPNGAPRAGAFSPEISWRWLRDELDTIDSRAQDPF
HLAEEDKKILCEEIFPYWAGKSVDEYCEAQYREAGLWELSGESYVSDCSYHALNGGGDSNPGYDVILMKKGMLDIQREAR
EHLEQLSYDRPEDIDKIYFYKSVIETTEGVMIYARRMSEYAAQLAARENDPRRKAELLKIAEVNARVPAHAPTTFWEAIQ
AVWTVESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTPYEAFDLAGCMLIKMSEMMWLTSEGSSKFFAGYQPFVNM
CVGGVSREGRDATNDLTYLLMDAVRHVRIYQPSLATRVHNTSPQEYLKKIVSVIRSGMGFPAVHFDDTHIKMMLAKGVDI
EDARDYCLMGCVEPQKSGRLYQWTSTAYTQWPICIELVLNRGVPLWYGKQVCPDLGDLSQFDTFEKFDAAVKEQIRYITK
WSSVATVISQRVHRDLAPKPLMSIMYEGCMEKGRDVAAGGAMYNFGPGVIWSGLATYADSMAAIKKLVYDDRKYTLQQLN
EALKANFEGYDAILADCLAAPKYGNDDDYADDIAADLIAFTEREHRKYRTLYSILSHGTLSISNNTPFGQLLGASAGGRK
AWQPLSDGISPTQGADYKGPTAIIKSVSKMSNDNMNIGMVHNFKLMPGLLDTPEGEQGIITLIRSASILGNGEMQFNYLD
NKTMLEAQQNPKDYRDLVVRVAGYSAFFVELCKDVQDEIISRTVLRHF

Sequences:

>Translated_848_residues
MDLHDFSSKIAEVTKNLSPEEREQLRKIFATVTLPAQSAPSAAAQPSPAAHSGPGVPEGPTQRHVLLKENYLKQVPRITI
HRARTITKIDKENPGMPRILLRATAFRHCCESAPLIIQDHELIVGAPNGAPRAGAFSPEISWRWLRDELDTIDSRAQDPF
HLAEEDKKILCEEIFPYWAGKSVDEYCEAQYREAGLWELSGESYVSDCSYHALNGGGDSNPGYDVILMKKGMLDIQREAR
EHLEQLSYDRPEDIDKIYFYKSVIETTEGVMIYARRMSEYAAQLAARENDPRRKAELLKIAEVNARVPAHAPTTFWEAIQ
AVWTVESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTPYEAFDLAGCMLIKMSEMMWLTSEGSSKFFAGYQPFVNM
CVGGVSREGRDATNDLTYLLMDAVRHVRIYQPSLATRVHNTSPQEYLKKIVSVIRSGMGFPAVHFDDTHIKMMLAKGVDI
EDARDYCLMGCVEPQKSGRLYQWTSTAYTQWPICIELVLNRGVPLWYGKQVCPDLGDLSQFDTFEKFDAAVKEQIRYITK
WSSVATVISQRVHRDLAPKPLMSIMYEGCMEKGRDVAAGGAMYNFGPGVIWSGLATYADSMAAIKKLVYDDRKYTLQQLN
EALKANFEGYDAILADCLAAPKYGNDDDYADDIAADLIAFTEREHRKYRTLYSILSHGTLSISNNTPFGQLLGASAGGRK
AWQPLSDGISPTQGADYKGPTAIIKSVSKMSNDNMNIGMVHNFKLMPGLLDTPEGEQGIITLIRSASILGNGEMQFNYLD
NKTMLEAQQNPKDYRDLVVRVAGYSAFFVELCKDVQDEIISRTVLRHF
>Mature_848_residues
MDLHDFSSKIAEVTKNLSPEEREQLRKIFATVTLPAQSAPSAAAQPSPAAHSGPGVPEGPTQRHVLLKENYLKQVPRITI
HRARTITKIDKENPGMPRILLRATAFRHCCESAPLIIQDHELIVGAPNGAPRAGAFSPEISWRWLRDELDTIDSRAQDPF
HLAEEDKKILCEEIFPYWAGKSVDEYCEAQYREAGLWELSGESYVSDCSYHALNGGGDSNPGYDVILMKKGMLDIQREAR
EHLEQLSYDRPEDIDKIYFYKSVIETTEGVMIYARRMSEYAAQLAARENDPRRKAELLKIAEVNARVPAHAPTTFWEAIQ
AVWTVESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTPYEAFDLAGCMLIKMSEMMWLTSEGSSKFFAGYQPFVNM
CVGGVSREGRDATNDLTYLLMDAVRHVRIYQPSLATRVHNTSPQEYLKKIVSVIRSGMGFPAVHFDDTHIKMMLAKGVDI
EDARDYCLMGCVEPQKSGRLYQWTSTAYTQWPICIELVLNRGVPLWYGKQVCPDLGDLSQFDTFEKFDAAVKEQIRYITK
WSSVATVISQRVHRDLAPKPLMSIMYEGCMEKGRDVAAGGAMYNFGPGVIWSGLATYADSMAAIKKLVYDDRKYTLQQLN
EALKANFEGYDAILADCLAAPKYGNDDDYADDIAADLIAFTEREHRKYRTLYSILSHGTLSISNNTPFGQLLGASAGGRK
AWQPLSDGISPTQGADYKGPTAIIKSVSKMSNDNMNIGMVHNFKLMPGLLDTPEGEQGIITLIRSASILGNGEMQFNYLD
NKTMLEAQQNPKDYRDLVVRVAGYSAFFVELCKDVQDEIISRTVLRHF

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=794, Percent_Identity=34.5088161209068, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1787044, Length=792, Percent_Identity=31.5656565656566, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1787131, Length=552, Percent_Identity=24.4565217391304, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI48994926, Length=589, Percent_Identity=22.7504244482173, Blast_Score=129, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 95209; Mature: 95209

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLHDFSSKIAEVTKNLSPEEREQLRKIFATVTLPAQSAPSAAAQPSPAAHSGPGVPEGP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TQRHVLLKENYLKQVPRITIHRARTITKIDKENPGMPRILLRATAFRHCCESAPLIIQDH
CCCEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
ELIVGAPNGAPRAGAFSPEISWRWLRDELDTIDSRAQDPFHLAEEDKKILCEEIFPYWAG
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
KSVDEYCEAQYREAGLWELSGESYVSDCSYHALNGGGDSNPGYDVILMKKGMLDIQREAR
CCHHHHHHHHHHHCCCEEECCCHHHHCCCCEECCCCCCCCCCEEEEEEECCCHHHHHHHH
EHLEQLSYDRPEDIDKIYFYKSVIETTEGVMIYARRMSEYAAQLAARENDPRRKAELLKI
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AEVNARVPAHAPTTFWEAIQAVWTVESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRM
HHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCHHHHCCCCCCC
TPYEAFDLAGCMLIKMSEMMWLTSEGSSKFFAGYQPFVNMCVGGVSREGRDATNDLTYLL
CCHHHHHHHHHHHHHHHHHHEEECCCCCCEECCHHHHHHHHHCCCCCCCCCCHHHHHHHH
MDAVRHVRIYQPSLATRVHNTSPQEYLKKIVSVIRSGMGFPAVHFDDTHIKMMLAKGVDI
HHHHHHHEEECCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHEEHHHCCCCH
EDARDYCLMGCVEPQKSGRLYQWTSTAYTQWPICIELVLNRGVPLWYGKQVCPDLGDLSQ
HHCCCHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEECHHHCCCCCCHHH
FDTFEKFDAAVKEQIRYITKWSSVATVISQRVHRDLAPKPLMSIMYEGCMEKGRDVAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECCC
AMYNFGPGVIWSGLATYADSMAAIKKLVYDDRKYTLQQLNEALKANFEGYDAILADCLAA
CCEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
PKYGNDDDYADDIAADLIAFTEREHRKYRTLYSILSHGTLSISNNTPFGQLLGASAGGRK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCCCCH
AWQPLSDGISPTQGADYKGPTAIIKSVSKMSNDNMNIGMVHNFKLMPGLLDTPEGEQGII
HCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCEECCCCCCCCCCCCHHH
TLIRSASILGNGEMQFNYLDNKTMLEAQQNPKDYRDLVVRVAGYSAFFVELCKDVQDEII
HHHHHHHHCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
SRTVLRHF
HHHHHHCC
>Mature Secondary Structure
MDLHDFSSKIAEVTKNLSPEEREQLRKIFATVTLPAQSAPSAAAQPSPAAHSGPGVPEGP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TQRHVLLKENYLKQVPRITIHRARTITKIDKENPGMPRILLRATAFRHCCESAPLIIQDH
CCCEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
ELIVGAPNGAPRAGAFSPEISWRWLRDELDTIDSRAQDPFHLAEEDKKILCEEIFPYWAG
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
KSVDEYCEAQYREAGLWELSGESYVSDCSYHALNGGGDSNPGYDVILMKKGMLDIQREAR
CCHHHHHHHHHHHCCCEEECCCHHHHCCCCEECCCCCCCCCCEEEEEEECCCHHHHHHHH
EHLEQLSYDRPEDIDKIYFYKSVIETTEGVMIYARRMSEYAAQLAARENDPRRKAELLKI
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AEVNARVPAHAPTTFWEAIQAVWTVESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRM
HHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCHHHHCCCCCCC
TPYEAFDLAGCMLIKMSEMMWLTSEGSSKFFAGYQPFVNMCVGGVSREGRDATNDLTYLL
CCHHHHHHHHHHHHHHHHHHEEECCCCCCEECCHHHHHHHHHCCCCCCCCCCHHHHHHHH
MDAVRHVRIYQPSLATRVHNTSPQEYLKKIVSVIRSGMGFPAVHFDDTHIKMMLAKGVDI
HHHHHHHEEECCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHEEHHHCCCCH
EDARDYCLMGCVEPQKSGRLYQWTSTAYTQWPICIELVLNRGVPLWYGKQVCPDLGDLSQ
HHCCCHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEECHHHCCCCCCHHH
FDTFEKFDAAVKEQIRYITKWSSVATVISQRVHRDLAPKPLMSIMYEGCMEKGRDVAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECCC
AMYNFGPGVIWSGLATYADSMAAIKKLVYDDRKYTLQQLNEALKANFEGYDAILADCLAA
CCEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
PKYGNDDDYADDIAADLIAFTEREHRKYRTLYSILSHGTLSISNNTPFGQLLGASAGGRK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCCCCH
AWQPLSDGISPTQGADYKGPTAIIKSVSKMSNDNMNIGMVHNFKLMPGLLDTPEGEQGII
HCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCEECCCCCCCCCCCCHHH
TLIRSASILGNGEMQFNYLDNKTMLEAQQNPKDYRDLVVRVAGYSAFFVELCKDVQDEII
HHHHHHHHCCCCCEEEEECCCCHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
SRTVLRHF
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]