Click here to switch to the map view.

The map label for this gene is adhE [H]

Identifier: 220904624

GI number: 220904624

Start: 1629893

End: 1631371

Strand: Direct

Name: adhE [H]

Synonym: Ddes_1356

Alternate gene names: 220904624

Gene position: 1629893-1631371 (Clockwise)

Preceding gene: 220904623

Following gene: 220904625

Centisome position: 56.72

GC content: 59.97

Gene sequence:

>1479_bases
ATGATCGGCGACAAGGACCTCATTTCTATACAGCAGGCGCGCATTCTGGCCGAAAATGCCATTGAAGCCGGCAAAAAACT
GGCCGCCCTGCCACAAGAGAATCTGGATAGCATTGTTGAAGCCATGGCAGACGCTGTGGAAACGCATGCGCAGTCCCTTG
CCGTCATGTGTCATGAAGAAAGCGACTGTGGCCGCTGGCAGGACAAAATGGCCAAAAACCTTTTTGTCTGTCGCCGGGTC
CGGCAGAGCCTGCGCGGCATGCGCTGCGTCGGCTATCTTGGCGGGCAGGAGCAGGAAGGCGTTTATGACGTTGGTGTGCC
CCTGGGCGTCATTGTAGCCCTCTGCCCGGTGACCAGTCCTGTATCTACCGCCATCTATAACGTTCTGCTGGCCATCAAGT
CCGGCAATGCCATAATTTTTTCCCTCCACCCCCGTGCTGTGGACAGCATGCGCCACGCCCTGGATATCATGATTGCCGCC
GGTCAGGCCCACGGCCTGCCCGAAGGGGCCATATCCTACCTGGACATTGTGGCAAAAAGCGGCACGGAAGAACTCATGCG
CCACCCCGGCGTGGCCCTGGTCATGGTTACGGGCGTGCTCGGCATGTTCGAGGCTGCCCGCACCAGCGGCAAGCCCCTCA
TCTATGGCGGCACAGGTAACGGCCCGGTTTTCATCGAGCGCAGCGCCGACATTCCCGCCGCCGTGGAGCATATTATCGCC
AGCAAAACCTTTGATAACGGGCTGGCGCCTTCAGCCGAGCAGTGCGTCATCGTGGATGGCTGTGTTGAGCGGCAGACCCG
CCGGGCCTTTCAGGACAAAGGCGCATATTTCATGAGCGAGGCCGAGGCGCAGGCCCTGTCACGGGTACTCTTTCATGAAG
ACGGACGCCGTCACCGCCACATGGTGGGCCAGTCTGCCGAGGCCCTTGCAACCAAGGCCGGCTTTTCCATTCCTGCCGAT
ACAAAAGTGCTCATGGCTGAACGCAAGTACGTTACAGGCGAGGATCCCTATGTCAGGGAACTGCTCTCGCCCGTCCTCGG
TTATTACGTGGAACCTGACTGGATGCACGCCTGCGAAAAGTGCGTGGAACTGCTGCTTCAGGAGCGGCATGCGCAAACTC
TGGCTATCCATAGCCGGGATGAAGAAGTCATCCGCCAGTTTGCCCTCAAAAAACCCGTGGCGCGCCTGCTGGTCAACACC
GGCGCTGCTTTTGGCGGCATGGGGCTGACAACCAACCTTACCCCCGCCATGACGCAGGGCAGTGGCATTGCGGGCTACGG
CATCACATCAGACAATATTTCGCCCATGAATCTCATTTATCGCAGAAAGATAGGCTATGGCGTGCGTGATCTCGCCCAGC
TGGCGGATTTCACCGCACCGTCCGCCCCCGGCGGGGGCGCGACCGGCAACGAGCAGAACTTTGACCTGCTCAGACGCGTA
CTGCGCGAGGCTCTGGGCGCTCTGCCCCAGGCTCGCTGA

Upstream 100 bases:

>100_bases
TCTACAGTTCTTGCGTCATCCCCACCCCTCACCGGCATCTTGTAAAACTTGTACGGCGGTACACCCTGGCCTAGCCGGGC
ACAGACCACGGAGCAGCATC

Downstream 100 bases:

>100_bases
CCCCGGGGCACACACCAAGCCCGCAGGCCCTTCTGACCGCAGAATACGGAGGATAGAACGTGGATCTTCATGATTTTTCG
AGCAAAATAGCGGAAGTTAC

Product: aldehyde-alcohol dehydrogenase 2

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MIGDKDLISIQQARILAENAIEAGKKLAALPQENLDSIVEAMADAVETHAQSLAVMCHEESDCGRWQDKMAKNLFVCRRV
RQSLRGMRCVGYLGGQEQEGVYDVGVPLGVIVALCPVTSPVSTAIYNVLLAIKSGNAIIFSLHPRAVDSMRHALDIMIAA
GQAHGLPEGAISYLDIVAKSGTEELMRHPGVALVMVTGVLGMFEAARTSGKPLIYGGTGNGPVFIERSADIPAAVEHIIA
SKTFDNGLAPSAEQCVIVDGCVERQTRRAFQDKGAYFMSEAEAQALSRVLFHEDGRRHRHMVGQSAEALATKAGFSIPAD
TKVLMAERKYVTGEDPYVRELLSPVLGYYVEPDWMHACEKCVELLLQERHAQTLAIHSRDEEVIRQFALKKPVARLLVNT
GAAFGGMGLTTNLTPAMTQGSGIAGYGITSDNISPMNLIYRRKIGYGVRDLAQLADFTAPSAPGGGATGNEQNFDLLRRV
LREALGALPQAR

Sequences:

>Translated_492_residues
MIGDKDLISIQQARILAENAIEAGKKLAALPQENLDSIVEAMADAVETHAQSLAVMCHEESDCGRWQDKMAKNLFVCRRV
RQSLRGMRCVGYLGGQEQEGVYDVGVPLGVIVALCPVTSPVSTAIYNVLLAIKSGNAIIFSLHPRAVDSMRHALDIMIAA
GQAHGLPEGAISYLDIVAKSGTEELMRHPGVALVMVTGVLGMFEAARTSGKPLIYGGTGNGPVFIERSADIPAAVEHIIA
SKTFDNGLAPSAEQCVIVDGCVERQTRRAFQDKGAYFMSEAEAQALSRVLFHEDGRRHRHMVGQSAEALATKAGFSIPAD
TKVLMAERKYVTGEDPYVRELLSPVLGYYVEPDWMHACEKCVELLLQERHAQTLAIHSRDEEVIRQFALKKPVARLLVNT
GAAFGGMGLTTNLTPAMTQGSGIAGYGITSDNISPMNLIYRRKIGYGVRDLAQLADFTAPSAPGGGATGNEQNFDLLRRV
LREALGALPQAR
>Mature_492_residues
MIGDKDLISIQQARILAENAIEAGKKLAALPQENLDSIVEAMADAVETHAQSLAVMCHEESDCGRWQDKMAKNLFVCRRV
RQSLRGMRCVGYLGGQEQEGVYDVGVPLGVIVALCPVTSPVSTAIYNVLLAIKSGNAIIFSLHPRAVDSMRHALDIMIAA
GQAHGLPEGAISYLDIVAKSGTEELMRHPGVALVMVTGVLGMFEAARTSGKPLIYGGTGNGPVFIERSADIPAAVEHIIA
SKTFDNGLAPSAEQCVIVDGCVERQTRRAFQDKGAYFMSEAEAQALSRVLFHEDGRRHRHMVGQSAEALATKAGFSIPAD
TKVLMAERKYVTGEDPYVRELLSPVLGYYVEPDWMHACEKCVELLLQERHAQTLAIHSRDEEVIRQFALKKPVARLLVNT
GAAFGGMGLTTNLTPAMTQGSGIAGYGITSDNISPMNLIYRRKIGYGVRDLAQLADFTAPSAPGGGATGNEQNFDLLRRV
LREALGALPQAR

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=443, Percent_Identity=35.4401805869075, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI1788797, Length=380, Percent_Identity=30.7894736842105, Blast_Score=149, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 53067; Mature: 53067

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGDKDLISIQQARILAENAIEAGKKLAALPQENLDSIVEAMADAVETHAQSLAVMCHEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDCGRWQDKMAKNLFVCRRVRQSLRGMRCVGYLGGQEQEGVYDVGVPLGVIVALCPVTSP
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEECCCHHHHHHHHHCCCCH
VSTAIYNVLLAIKSGNAIIFSLHPRAVDSMRHALDIMIAAGQAHGLPEGAISYLDIVAKS
HHHHHHHHHHEEECCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
GTEELMRHPGVALVMVTGVLGMFEAARTSGKPLIYGGTGNGPVFIERSADIPAAVEHIIA
CHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHH
SKTFDNGLAPSAEQCVIVDGCVERQTRRAFQDKGAYFMSEAEAQALSRVLFHEDGRRHRH
HHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
MVGQSAEALATKAGFSIPADTKVLMAERKYVTGEDPYVRELLSPVLGYYVEPDWMHACEK
HHCCCHHHHHHHCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
CVELLLQERHAQTLAIHSRDEEVIRQFALKKPVARLLVNTGAAFGGMGLTTNLTPAMTQG
HHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SGIAGYGITSDNISPMNLIYRRKIGYGVRDLAQLADFTAPSAPGGGATGNEQNFDLLRRV
CCCEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
LREALGALPQAR
HHHHHHCCCCCC
>Mature Secondary Structure
MIGDKDLISIQQARILAENAIEAGKKLAALPQENLDSIVEAMADAVETHAQSLAVMCHEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDCGRWQDKMAKNLFVCRRVRQSLRGMRCVGYLGGQEQEGVYDVGVPLGVIVALCPVTSP
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEECCCHHHHHHHHHCCCCH
VSTAIYNVLLAIKSGNAIIFSLHPRAVDSMRHALDIMIAAGQAHGLPEGAISYLDIVAKS
HHHHHHHHHHEEECCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
GTEELMRHPGVALVMVTGVLGMFEAARTSGKPLIYGGTGNGPVFIERSADIPAAVEHIIA
CHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHH
SKTFDNGLAPSAEQCVIVDGCVERQTRRAFQDKGAYFMSEAEAQALSRVLFHEDGRRHRH
HHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHH
MVGQSAEALATKAGFSIPADTKVLMAERKYVTGEDPYVRELLSPVLGYYVEPDWMHACEK
HHCCCHHHHHHHCCCCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
CVELLLQERHAQTLAIHSRDEEVIRQFALKKPVARLLVNTGAAFGGMGLTTNLTPAMTQG
HHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SGIAGYGITSDNISPMNLIYRRKIGYGVRDLAQLADFTAPSAPGGGATGNEQNFDLLRRV
CCCEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
LREALGALPQAR
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]