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The map label for this gene is yogA [H]

Identifier: 220904600

GI number: 220904600

Start: 1583087

End: 1584412

Strand: Reverse

Name: yogA [H]

Synonym: Ddes_1332

Alternate gene names: 220904600

Gene position: 1584412-1583087 (Counterclockwise)

Preceding gene: 220904601

Following gene: 220904599

Centisome position: 55.14

GC content: 53.17

Gene sequence:

>1326_bases
ATGAACAGTTTGAGTAGTGTTTCAGATGCCATTACGCCCCTTGAAAAACAGAAAGTCATGCGGGCTGTGGTGCTCTATAA
AGCTGAAGATGAAAAGCTCATGGCAATTGCCGATGAAGACGAGCGCCTCAGGGCCGCCATTCACGTTGAGCGTGATATCC
CCATTCCTGTCCCCGGTACGGGCGAGGTGCTTGTAAAGGTTATGGCCGGAGCTCTTAATTTCAACACCCTGTGGGCGCTG
AAACGCAAACCGGTGAGCACGTTCGACCGTCTGGCGAGATTTGCCAAACTTGATCCTGACGCAGTCCGTCATAATCTCGA
CTACCAGATCATGGGCAGCGATGGCGCAGGCATAGTGGTCAAGGTGGGCGAAGGCGTTACAAACTGCCGCCCCGGCGATC
ACATCGTTGTTTATCCCGTGGTGTTCAATCATCAGTCACCAGAGGCAATGGAAGACTACCTGTTTGACGATCACAGCCGC
ATATGGGGTTACGAAACAAATTTCGGTTCTTTCTCGGATTATTGCCTTATCCGTGCAACTCAGGTTCTGCCTAAACCCAG
GCATCTTTCCTGGGCCGAAGCGGCGGCTCTGCCAGGGGCCAATTCCACTGTGTACAGAATGCTGATCTCGCCCAACGGGG
CGCAGATTAAAATTGGCGAGAGCGTCCTTATCTGGGGAGCCAGCGGCGGCATCGGCAGCATGGCTGTGCAGTATGCGCTC
AGAACCGGGGCTTTTCCTGTTGCTGTCGTTTCTTCCGAAGAAAAGGCCGAGCTGGTGAGGGCGCTTGGTTGCCGCGCGGT
GATTGTTCGCCCCAACGAAGAAAACGAATTCGTCAATGACGACGGTACACCTGATATGCGGCGTCTTGTGCGCTTTCGGC
GTGAAGTATTGCAGGCCAACGGAGGCAAGGCGCCGGATGTGGTTTTTGAGCATCCGGGGCGCGGCACGTTTTACGCCAGC
GTATTTGTGGCTGCCAACCGGGGGCGCATTGTCACCTGCGGGTCCACAACTGGTTATGACCACCTCTACGACAACCGGTA
TCTCTGGATGTACGGCAAGCGCATTATCGGTTCCCACTGCGCCTCCTGGAATGAAGCTGCGCGGGCTAATGATCTCATAT
GCAAGGGATTGATCATGCCGGAGCTCAGTAGTGCGTTTTCGTTGGACAGGTTTGAAGACGCTGTTGAAGAGTTGAAAAAT
AATCATGTGGGCAAGGTAGCCATACTTTGCAATGCCGAAACGATTGATGAAGGGGTTGAAGATATGGAATTTCGCAATGC
CATTGGCGAAAAACAGTTCAGAATATTCCGCAAAAAGGGAAAATGA

Upstream 100 bases:

>100_bases
TGCACATGATGCCGGGAGGGCATTTTTTCATCCAGACGCATTCCGAGCTTGTTATGCGCAGTGTTTTTGACCATCTGAAA
ATTTTATAAAGGAGCGGCCC

Downstream 100 bases:

>100_bases
TGTCTACTGCGCCAGAAAAAATGCAAAGCCCGCTTACTGTCTACTGGCAGGCTGCCATGCCGGAGTGGGGCAAACTTTCG
GTGACGCTTTTGCTTGCCTG

Product: crotonyl-CoA reductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNSLSSVSDAITPLEKQKVMRAVVLYKAEDEKLMAIADEDERLRAAIHVERDIPIPVPGTGEVLVKVMAGALNFNTLWAL
KRKPVSTFDRLARFAKLDPDAVRHNLDYQIMGSDGAGIVVKVGEGVTNCRPGDHIVVYPVVFNHQSPEAMEDYLFDDHSR
IWGYETNFGSFSDYCLIRATQVLPKPRHLSWAEAAALPGANSTVYRMLISPNGAQIKIGESVLIWGASGGIGSMAVQYAL
RTGAFPVAVVSSEEKAELVRALGCRAVIVRPNEENEFVNDDGTPDMRRLVRFRREVLQANGGKAPDVVFEHPGRGTFYAS
VFVAANRGRIVTCGSTTGYDHLYDNRYLWMYGKRIIGSHCASWNEAARANDLICKGLIMPELSSAFSLDRFEDAVEELKN
NHVGKVAILCNAETIDEGVEDMEFRNAIGEKQFRIFRKKGK

Sequences:

>Translated_441_residues
MNSLSSVSDAITPLEKQKVMRAVVLYKAEDEKLMAIADEDERLRAAIHVERDIPIPVPGTGEVLVKVMAGALNFNTLWAL
KRKPVSTFDRLARFAKLDPDAVRHNLDYQIMGSDGAGIVVKVGEGVTNCRPGDHIVVYPVVFNHQSPEAMEDYLFDDHSR
IWGYETNFGSFSDYCLIRATQVLPKPRHLSWAEAAALPGANSTVYRMLISPNGAQIKIGESVLIWGASGGIGSMAVQYAL
RTGAFPVAVVSSEEKAELVRALGCRAVIVRPNEENEFVNDDGTPDMRRLVRFRREVLQANGGKAPDVVFEHPGRGTFYAS
VFVAANRGRIVTCGSTTGYDHLYDNRYLWMYGKRIIGSHCASWNEAARANDLICKGLIMPELSSAFSLDRFEDAVEELKN
NHVGKVAILCNAETIDEGVEDMEFRNAIGEKQFRIFRKKGK
>Mature_441_residues
MNSLSSVSDAITPLEKQKVMRAVVLYKAEDEKLMAIADEDERLRAAIHVERDIPIPVPGTGEVLVKVMAGALNFNTLWAL
KRKPVSTFDRLARFAKLDPDAVRHNLDYQIMGSDGAGIVVKVGEGVTNCRPGDHIVVYPVVFNHQSPEAMEDYLFDDHSR
IWGYETNFGSFSDYCLIRATQVLPKPRHLSWAEAAALPGANSTVYRMLISPNGAQIKIGESVLIWGASGGIGSMAVQYAL
RTGAFPVAVVSSEEKAELVRALGCRAVIVRPNEENEFVNDDGTPDMRRLVRFRREVLQANGGKAPDVVFEHPGRGTFYAS
VFVAANRGRIVTCGSTTGYDHLYDNRYLWMYGKRIIGSHCASWNEAARANDLICKGLIMPELSSAFSLDRFEDAVEELKN
NHVGKVAILCNAETIDEGVEDMEFRNAIGEKQFRIFRKKGK

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=319, Percent_Identity=25.705329153605, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI13236495, Length=319, Percent_Identity=25.705329153605, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI194239676, Length=251, Percent_Identity=27.8884462151394, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1790485, Length=228, Percent_Identity=27.6315789473684, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71988145, Length=359, Percent_Identity=23.3983286908078, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324486, Length=357, Percent_Identity=26.6106442577031, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323961, Length=380, Percent_Identity=24.2105263157895, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=220, Percent_Identity=30, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=386, Percent_Identity=24.3523316062176, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 48857; Mature: 48857

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLSSVSDAITPLEKQKVMRAVVLYKAEDEKLMAIADEDERLRAAIHVERDIPIPVPGT
CCCHHHHHHHHCCHHHHHHHHHHHEEECCCCEEEEEECCCHHEEEEEEEECCCCCCCCCC
GEVLVKVMAGALNFNTLWALKRKPVSTFDRLARFAKLDPDAVRHNLDYQIMGSDGAGIVV
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCEEEE
KVGEGVTNCRPGDHIVVYPVVFNHQSPEAMEDYLFDDHSRIWGYETNFGSFSDYCLIRAT
ECCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEHH
QVLPKPRHLSWAEAAALPGANSTVYRMLISPNGAQIKIGESVLIWGASGGIGSMAVQYAL
HHCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCEEEECCEEEEEECCCCHHHHHHHHHH
RTGAFPVAVVSSEEKAELVRALGCRAVIVRPNEENEFVNDDGTPDMRRLVRFRREVLQAN
HCCCCEEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
GGKAPDVVFEHPGRGTFYASVFVAANRGRIVTCGSTTGYDHLYDNRYLWMYGKRIIGSHC
CCCCCCEEEECCCCCEEEEEEEEEECCCEEEEECCCCCCCHHHCCCEEEEECHHHHHHHH
ASWNEAARANDLICKGLIMPELSSAFSLDRFEDAVEELKNNHVGKVAILCNAETIDEGVE
CCCHHHHHCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCHH
DMEFRNAIGEKQFRIFRKKGK
HHHHHHHHCHHHHHHHHHCCC
>Mature Secondary Structure
MNSLSSVSDAITPLEKQKVMRAVVLYKAEDEKLMAIADEDERLRAAIHVERDIPIPVPGT
CCCHHHHHHHHCCHHHHHHHHHHHEEECCCCEEEEEECCCHHEEEEEEEECCCCCCCCCC
GEVLVKVMAGALNFNTLWALKRKPVSTFDRLARFAKLDPDAVRHNLDYQIMGSDGAGIVV
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCEEEE
KVGEGVTNCRPGDHIVVYPVVFNHQSPEAMEDYLFDDHSRIWGYETNFGSFSDYCLIRAT
ECCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEHH
QVLPKPRHLSWAEAAALPGANSTVYRMLISPNGAQIKIGESVLIWGASGGIGSMAVQYAL
HHCCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCEEEECCEEEEEECCCCHHHHHHHHHH
RTGAFPVAVVSSEEKAELVRALGCRAVIVRPNEENEFVNDDGTPDMRRLVRFRREVLQAN
HCCCCEEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
GGKAPDVVFEHPGRGTFYASVFVAANRGRIVTCGSTTGYDHLYDNRYLWMYGKRIIGSHC
CCCCCCEEEECCCCCEEEEEEEEEECCCEEEEECCCCCCCHHHCCCEEEEECHHHHHHHH
ASWNEAARANDLICKGLIMPELSSAFSLDRFEDAVEELKNNHVGKVAILCNAETIDEGVE
CCCHHHHHCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCHH
DMEFRNAIGEKQFRIFRKKGK
HHHHHHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]