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The map label for this gene is lgrE [H]

Identifier: 220904601

GI number: 220904601

Start: 1584424

End: 1585167

Strand: Reverse

Name: lgrE [H]

Synonym: Ddes_1333

Alternate gene names: 220904601

Gene position: 1585167-1584424 (Counterclockwise)

Preceding gene: 220904602

Following gene: 220904600

Centisome position: 55.17

GC content: 56.72

Gene sequence:

>744_bases
ATGCCTGAAACCAGCCCCTGGCTTATCTTTGACAAGAAACCCGGCCCAGATGACCTGATCCTGTTCTGCCTGCATTTTGC
AGGCGGGCATGCGGCCCTTTTCAGGCAATGGCAGCAACATGCTCCGGCAGGCGTGGTTGTCTGCCGGGTGCAGATGCCCG
GTCACGGCACACGCATGGCTGAAGAGCTCCTGACGGACATGTCTTCGCTTATCGACGGTTTGCAGGAGGCCGCGCAATGC
TGGATCGGGCATCGTCACGCCTTTTTCGGCCATTCAATGGGCGGAACCGTTGCTGCGGAGTGGGCGATACGTCATCAGGG
CGAAGGCTTGCCTTTGCCCGAGTGCCTTTTTGTCAGCGCATCGGAACCCACGCACGAAAACTGGTGGCCTCCCTGCCGGG
ATCTTCCCTCAGATGCGTTCAGGTCTTTCATTTTTAAGACCGGTGGCGTGCCCAAGGAAATTCTGGAGCATGAAGAGCTG
ATGGCACTTTTTGAACCCATCATCCGGGCGGACTATACCGTGCTGGAAACTTGGGCTCCAAAGCCGGTGCGTCCTATCCA
CGTGCCAATAGTCGCCTTTGCCGGTCTGGATGACCACGTTGTTCCCGTGGAGGTGGTGGCGGAGTGGGAACGCTTTGCCG
CGGCCTCGTGGGAAATGCACATGATGCCGGGAGGGCATTTTTTCATCCAGACGCATTCCGAGCTTGTTATGCGCAGTGTT
TTTGACCATCTGAAAATTTTATAA

Upstream 100 bases:

>100_bases
TCTTCACCGGGTAATCACGCCAACTGCCTGAAGCGCAAGCAGCTGCTTGAGTGGATAGGGCGGCTGATTGACTGGTACGA
AGCGGACGGGGAAGAGCAGC

Downstream 100 bases:

>100_bases
AGGAGCGGCCCATGAACAGTTTGAGTAGTGTTTCAGATGCCATTACGCCCCTTGAAAAACAGAAAGTCATGCGGGCTGTG
GTGCTCTATAAAGCTGAAGA

Product: Thioesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MPETSPWLIFDKKPGPDDLILFCLHFAGGHAALFRQWQQHAPAGVVVCRVQMPGHGTRMAEELLTDMSSLIDGLQEAAQC
WIGHRHAFFGHSMGGTVAAEWAIRHQGEGLPLPECLFVSASEPTHENWWPPCRDLPSDAFRSFIFKTGGVPKEILEHEEL
MALFEPIIRADYTVLETWAPKPVRPIHVPIVAFAGLDDHVVPVEVVAEWERFAAASWEMHMMPGGHFFIQTHSELVMRSV
FDHLKIL

Sequences:

>Translated_247_residues
MPETSPWLIFDKKPGPDDLILFCLHFAGGHAALFRQWQQHAPAGVVVCRVQMPGHGTRMAEELLTDMSSLIDGLQEAAQC
WIGHRHAFFGHSMGGTVAAEWAIRHQGEGLPLPECLFVSASEPTHENWWPPCRDLPSDAFRSFIFKTGGVPKEILEHEEL
MALFEPIIRADYTVLETWAPKPVRPIHVPIVAFAGLDDHVVPVEVVAEWERFAAASWEMHMMPGGHFFIQTHSELVMRSV
FDHLKIL
>Mature_246_residues
PETSPWLIFDKKPGPDDLILFCLHFAGGHAALFRQWQQHAPAGVVVCRVQMPGHGTRMAEELLTDMSSLIDGLQEAAQCW
IGHRHAFFGHSMGGTVAAEWAIRHQGEGLPLPECLFVSASEPTHENWWPPCRDLPSDAFRSFIFKTGGVPKEILEHEELM
ALFEPIIRADYTVLETWAPKPVRPIHVPIVAFAGLDDHVVPVEVVAEWERFAAASWEMHMMPGGHFFIQTHSELVMRSVF
DHLKIL

Specific function: In the final step of gramicidin biosynthesis, reduces the pentadecapeptide-aldehyde intermediate, that is released from the terminal module of the non-ribosomal peptide synthetase LgrD, to the final product ethanolamine-containing gramicidin [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase family [H]

Homologues:

Organism=Homo sapiens, GI89257335, Length=215, Percent_Identity=29.3023255813954, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI8922871, Length=186, Percent_Identity=29.5698924731183, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 27812; Mature: 27681

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETSPWLIFDKKPGPDDLILFCLHFAGGHAALFRQWQQHAPAGVVVCRVQMPGHGTRMA
CCCCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHH
EELLTDMSSLIDGLQEAAQCWIGHRHAFFGHSMGGTVAAEWAIRHQGEGLPLPECLFVSA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEC
SEPTHENWWPPCRDLPSDAFRSFIFKTGGVPKEILEHEELMALFEPIIRADYTVLETWAP
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCC
KPVRPIHVPIVAFAGLDDHVVPVEVVAEWERFAAASWEMHMMPGGHFFIQTHSELVMRSV
CCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEEECHHHHHHHHH
FDHLKIL
HHHHHCC
>Mature Secondary Structure 
PETSPWLIFDKKPGPDDLILFCLHFAGGHAALFRQWQQHAPAGVVVCRVQMPGHGTRMA
CCCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHH
EELLTDMSSLIDGLQEAAQCWIGHRHAFFGHSMGGTVAAEWAIRHQGEGLPLPECLFVSA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEC
SEPTHENWWPPCRDLPSDAFRSFIFKTGGVPKEILEHEELMALFEPIIRADYTVLETWAP
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCC
KPVRPIHVPIVAFAGLDDHVVPVEVVAEWERFAAASWEMHMMPGGHFFIQTHSELVMRSV
CCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCEEEEECHHHHHHHHH
FDHLKIL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA