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The map label for this gene is ykwC [H]

Identifier: 220904588

GI number: 220904588

Start: 1569759

End: 1570664

Strand: Direct

Name: ykwC [H]

Synonym: Ddes_1320

Alternate gene names: 220904588

Gene position: 1569759-1570664 (Clockwise)

Preceding gene: 220904587

Following gene: 220904589

Centisome position: 54.63

GC content: 58.17

Gene sequence:

>906_bases
ATGGCTGAAAAACTGCGCATAGGTTGGATTGGAACCGGGGTTATGGGTTTGTCCATGGCCGGTCACCTGCAGGATGCGGG
GCATAGCCTGACCGTGTACAACCGCACCCGTGCCAAGGCCGACCCGCTGCTTGCCAAGGGCGCTCAATGGGCCGATACGC
CGCGTCAGGCGGCAGCCAATGCCGATGTGGTGTTCACTATTGTCGGCTATCCGCAGGATGTGGAAAGCGTCATCCTGGGC
GAAGAAGGCGTATTGCAGGGCCTGCCCTCGGGCGGCATTGTCTGCGACATGAGCACTTCAAGCCCTTCTCTGGCCGAACG
CATCGCCGAGGCAGCCCTGCTGCAGAGCTGCCTGTCACTGGACGCCCCTGTAACAGGCGGCGATGTGGGCGCACGCAACG
CGACCCTCTCCATCTTTGTCGGCGGTGATAAGGATGGCTATGAAAGGCTTGAAAGCTGCTTTAAAAGCATGGGAAAAAAC
ATTCTGCACTGCGGTGGCCCCGGTTTTGGTCAAAAAGCCAAGCTGGCCAATCAGGTTGCCATTGCCGGCGTGATGTTCAG
CGTGTGCGAATCCTGCCTTTTTGCGCAGGAAGCCGGCCTGGATGTGGTCCAGTGGCTGGAACTGGTAGTTATGGGCGCTG
CGGGCAGCAAAGCTATGGATACCCTGGGGCGGCGCATTCTCAAAAACGACTTTGCCCCGGGCTTTTTTATCAACCACTTC
ATCAAAGACCTTGGGCTCTGCCTTGAAGAATGCCGTCGCATGCAGATCGTACTGCCCGGCATAGGCCTGGCCGAAGAACT
GTACCGCAGTATGCAGGCAAGAGGACAGGGCAACCTGGGAACCCAGGCACTTATTGACTGCCTTGCCTCCCTTTCAGGCA
AGCACTGGCAGCAACACAGCTCATAA

Upstream 100 bases:

>100_bases
AAACATGTTGACTTGTTTCACAAAAACAAAACATTGCCGCGAGAGGTCATGCAAAAAGAAATTCTAGAACGTCGCTTCCA
GTAGCCAAAGGAGTGCTAAC

Downstream 100 bases:

>100_bases
AAGGCTTTTTCCCGCCCGACGGCTCTCTCCGCCGGGCGGTTTCGTTTCTTCGTGACGTGCCTGCGCCCTCTGCTTTTTGG
GCCAACCAGCCAATGGAGGC

Product: 2-hydroxy-3-oxopropionate reductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MAEKLRIGWIGTGVMGLSMAGHLQDAGHSLTVYNRTRAKADPLLAKGAQWADTPRQAAANADVVFTIVGYPQDVESVILG
EEGVLQGLPSGGIVCDMSTSSPSLAERIAEAALLQSCLSLDAPVTGGDVGARNATLSIFVGGDKDGYERLESCFKSMGKN
ILHCGGPGFGQKAKLANQVAIAGVMFSVCESCLFAQEAGLDVVQWLELVVMGAAGSKAMDTLGRRILKNDFAPGFFINHF
IKDLGLCLEECRRMQIVLPGIGLAEELYRSMQARGQGNLGTQALIDCLASLSGKHWQQHSS

Sequences:

>Translated_301_residues
MAEKLRIGWIGTGVMGLSMAGHLQDAGHSLTVYNRTRAKADPLLAKGAQWADTPRQAAANADVVFTIVGYPQDVESVILG
EEGVLQGLPSGGIVCDMSTSSPSLAERIAEAALLQSCLSLDAPVTGGDVGARNATLSIFVGGDKDGYERLESCFKSMGKN
ILHCGGPGFGQKAKLANQVAIAGVMFSVCESCLFAQEAGLDVVQWLELVVMGAAGSKAMDTLGRRILKNDFAPGFFINHF
IKDLGLCLEECRRMQIVLPGIGLAEELYRSMQARGQGNLGTQALIDCLASLSGKHWQQHSS
>Mature_300_residues
AEKLRIGWIGTGVMGLSMAGHLQDAGHSLTVYNRTRAKADPLLAKGAQWADTPRQAAANADVVFTIVGYPQDVESVILGE
EGVLQGLPSGGIVCDMSTSSPSLAERIAEAALLQSCLSLDAPVTGGDVGARNATLSIFVGGDKDGYERLESCFKSMGKNI
LHCGGPGFGQKAKLANQVAIAGVMFSVCESCLFAQEAGLDVVQWLELVVMGAAGSKAMDTLGRRILKNDFAPGFFINHFI
KDLGLCLEECRRMQIVLPGIGLAEELYRSMQARGQGNLGTQALIDCLASLSGKHWQQHSS

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=273, Percent_Identity=30.03663003663, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI23308751, Length=282, Percent_Identity=27.6595744680851, Blast_Score=111, Evalue=7e-25,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=38.7323943661972, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=33.5714285714286, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1790315, Length=281, Percent_Identity=28.8256227758007, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789092, Length=293, Percent_Identity=26.962457337884, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17557316, Length=270, Percent_Identity=30.7407407407407, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24655230, Length=290, Percent_Identity=31.7241379310345, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922568, Length=290, Percent_Identity=31.7241379310345, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=27.0462633451957, Blast_Score=110, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31843; Mature: 31712

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKLRIGWIGTGVMGLSMAGHLQDAGHSLTVYNRTRAKADPLLAKGAQWADTPRQAAAN
CCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEECCHHHCCHHHHCCCCCCCCHHHHHCC
ADVVFTIVGYPQDVESVILGEEGVLQGLPSGGIVCDMSTSSPSLAERIAEAALLQSCLSL
CCEEEEEECCCCCHHHHHCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHC
DAPVTGGDVGARNATLSIFVGGDKDGYERLESCFKSMGKNILHCGGPGFGQKAKLANQVA
CCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHH
IAGVMFSVCESCLFAQEAGLDVVQWLELVVMGAAGSKAMDTLGRRILKNDFAPGFFINHF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
IKDLGLCLEECRRMQIVLPGIGLAEELYRSMQARGQGNLGTQALIDCLASLSGKHWQQHS
HHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHCCC
S
C
>Mature Secondary Structure 
AEKLRIGWIGTGVMGLSMAGHLQDAGHSLTVYNRTRAKADPLLAKGAQWADTPRQAAAN
CCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEECCHHHCCHHHHCCCCCCCCHHHHHCC
ADVVFTIVGYPQDVESVILGEEGVLQGLPSGGIVCDMSTSSPSLAERIAEAALLQSCLSL
CCEEEEEECCCCCHHHHHCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHC
DAPVTGGDVGARNATLSIFVGGDKDGYERLESCFKSMGKNILHCGGPGFGQKAKLANQVA
CCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHH
IAGVMFSVCESCLFAQEAGLDVVQWLELVVMGAAGSKAMDTLGRRILKNDFAPGFFINHF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
IKDLGLCLEECRRMQIVLPGIGLAEELYRSMQARGQGNLGTQALIDCLASLSGKHWQQHS
HHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]