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The map label for this gene is intS [H]

Identifier: 220904498

GI number: 220904498

Start: 1441887

End: 1443095

Strand: Reverse

Name: intS [H]

Synonym: Ddes_1229

Alternate gene names: 220904498

Gene position: 1443095-1441887 (Counterclockwise)

Preceding gene: 220904499

Following gene: 220904497

Centisome position: 50.22

GC content: 55.75

Gene sequence:

>1209_bases
TTGCCCCCAACGAACAAGCTCACCGAAATAAAAATCAGGAATATCAAGCCCAACGGCAAGATTGAACGCTTTGTCGATGG
GGAGGGGTTGTACCTCGAAACCTCTGTTGCCGGGGGCAAACACTGGCGCATGAAGTACCGGGTGAACGGCAAGGAAAAAC
GCCTTTCCTTCGGGCCGTGGCCGGATATTTCGCTGAAAGAAGCCCGTGAGATGCGGGATGAGGCAAAGAGGCAGTTACGG
GAAAGCCTTGACCCCTCAGCGGAAAAGAAAAAAGCCAAGCAGGAACAAGCAGCCCCTACGTTCCGTGACATTGCTCTGGA
GTATGTTGAGCACCAGCGTAGTGTCTGGGCCGCCAGTCATACCCGCACCGTTGAGGGGCGGCTGGCGCTGGACGTATACC
CCGCTTTTGGCGCAAAGCCCATTGCTGCCGTTACCCCTCAGGATATTCTCGCCATGCTCCGCAAAATTGAGGAGCGCCGT
GCCTTCGAGACCGCAAGCCGGGTCCTCGGTTTCTGCTCTCTCATTTTCAAATACGCGGTGACGCTCGGCCTTGTAGAAGC
CGATCCATGCCGCGACCTTCGCCCCGCCTTGAAGCCCTATGCCAAGGGCCAACTTGCCGCCATCACCAAGCCCAAGGAAG
TCGGCGCGCTCATGCGGGCCATTGATGACTACAAGGGCAGTGTTGTGGTGCGGGCTGCTCTGCTCTTTTCTGCGCTCACC
TTTTGTCGGCCCGGAGAAATCCGCCATGTGGAATGGGCTGAAATAAATTTTGAACGCGAAGAATGGACTATCCCCGCTGA
AAAAATGAAAGGCCGGACGGAACACCGGGTTCCTCTTTCACGCCAAGCCCTTGAGGTTTTGCACGGTATTATGCCCTACA
CAGGTTCCGCCAAGTACGTCTTCCCCACGCCGCGCAGCAATAACAGGCCGCTTTCTGAAAACGGCGTTTTGTCGGCCCTG
CGAAACATGGGGTACACCAAGGAACAAATGACCGCCCACGGTTTCCGCTCGATGGCTTCCACACTGCTCAACGAGCTTGG
GCACAGACCGGACGCCATTGAAGCCCAGCTTGCCCATAAGGGCGCGGATAAAATTCGGGCTACATATAACCGCGCTGAGT
ATATGAAGGAACGCCGCAACCTCATGCAAGCGTGGGCTGATTACTTAGAAGATTTAAAGATGCAGTCAATGCAGCCGGGG
CAAATATGA

Upstream 100 bases:

>100_bases
TTAGAGGCCCACGAGGTTTCACGCTGACCCTTGACATTCCACTCATGCTGGGGGCAAAAATGGGGGCAAAGGAGCAGGAA
AAAGCTCTAAGGAGGAAGTT

Downstream 100 bases:

>100_bases
TTAATCTCACCAGTGCTTTTGCCTATCAAAACATCGCCGTACATCCTGATGAACTTATGCCTATTGCTGAACTTATGCTG
CGTTGGGCATGGAATACGGA

Product: integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MPPTNKLTEIKIRNIKPNGKIERFVDGEGLYLETSVAGGKHWRMKYRVNGKEKRLSFGPWPDISLKEAREMRDEAKRQLR
ESLDPSAEKKKAKQEQAAPTFRDIALEYVEHQRSVWAASHTRTVEGRLALDVYPAFGAKPIAAVTPQDILAMLRKIEERR
AFETASRVLGFCSLIFKYAVTLGLVEADPCRDLRPALKPYAKGQLAAITKPKEVGALMRAIDDYKGSVVVRAALLFSALT
FCRPGEIRHVEWAEINFEREEWTIPAEKMKGRTEHRVPLSRQALEVLHGIMPYTGSAKYVFPTPRSNNRPLSENGVLSAL
RNMGYTKEQMTAHGFRSMASTLLNELGHRPDAIEAQLAHKGADKIRATYNRAEYMKERRNLMQAWADYLEDLKMQSMQPG
QI

Sequences:

>Translated_402_residues
MPPTNKLTEIKIRNIKPNGKIERFVDGEGLYLETSVAGGKHWRMKYRVNGKEKRLSFGPWPDISLKEAREMRDEAKRQLR
ESLDPSAEKKKAKQEQAAPTFRDIALEYVEHQRSVWAASHTRTVEGRLALDVYPAFGAKPIAAVTPQDILAMLRKIEERR
AFETASRVLGFCSLIFKYAVTLGLVEADPCRDLRPALKPYAKGQLAAITKPKEVGALMRAIDDYKGSVVVRAALLFSALT
FCRPGEIRHVEWAEINFEREEWTIPAEKMKGRTEHRVPLSRQALEVLHGIMPYTGSAKYVFPTPRSNNRPLSENGVLSAL
RNMGYTKEQMTAHGFRSMASTLLNELGHRPDAIEAQLAHKGADKIRATYNRAEYMKERRNLMQAWADYLEDLKMQSMQPG
QI
>Mature_401_residues
PPTNKLTEIKIRNIKPNGKIERFVDGEGLYLETSVAGGKHWRMKYRVNGKEKRLSFGPWPDISLKEAREMRDEAKRQLRE
SLDPSAEKKKAKQEQAAPTFRDIALEYVEHQRSVWAASHTRTVEGRLALDVYPAFGAKPIAAVTPQDILAMLRKIEERRA
FETASRVLGFCSLIFKYAVTLGLVEADPCRDLRPALKPYAKGQLAAITKPKEVGALMRAIDDYKGSVVVRAALLFSALTF
CRPGEIRHVEWAEINFEREEWTIPAEKMKGRTEHRVPLSRQALEVLHGIMPYTGSAKYVFPTPRSNNRPLSENGVLSALR
NMGYTKEQMTAHGFRSMASTLLNELGHRPDAIEAQLAHKGADKIRATYNRAEYMKERRNLMQAWADYLEDLKMQSMQPGQ
I

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=386, Percent_Identity=33.9378238341969, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI145693166, Length=401, Percent_Identity=37.4064837905237, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1788974, Length=392, Percent_Identity=31.8877551020408, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 45664; Mature: 45533

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPTNKLTEIKIRNIKPNGKIERFVDGEGLYLETSVAGGKHWRMKYRVNGKEKRLSFGPW
CCCCCCEEEEEEEECCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEECCCHHCCCCCCC
PDISLKEAREMRDEAKRQLRESLDPSAEKKKAKQEQAAPTFRDIALEYVEHQRSVWAASH
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
TRTVEGRLALDVYPAFGAKPIAAVTPQDILAMLRKIEERRAFETASRVLGFCSLIFKYAV
CEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGLVEADPCRDLRPALKPYAKGQLAAITKPKEVGALMRAIDDYKGSVVVRAALLFSALT
HHHCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FCRPGEIRHVEWAEINFEREEWTIPAEKMKGRTEHRVPLSRQALEVLHGIMPYTGSAKYV
HCCCCCCCEEEEHEEEECHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEE
FPTPRSNNRPLSENGVLSALRNMGYTKEQMTAHGFRSMASTLLNELGHRPDAIEAQLAHK
ECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
GADKIRATYNRAEYMKERRNLMQAWADYLEDLKMQSMQPGQI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PPTNKLTEIKIRNIKPNGKIERFVDGEGLYLETSVAGGKHWRMKYRVNGKEKRLSFGPW
CCCCCEEEEEEEECCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEECCCHHCCCCCCC
PDISLKEAREMRDEAKRQLRESLDPSAEKKKAKQEQAAPTFRDIALEYVEHQRSVWAASH
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
TRTVEGRLALDVYPAFGAKPIAAVTPQDILAMLRKIEERRAFETASRVLGFCSLIFKYAV
CEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLGLVEADPCRDLRPALKPYAKGQLAAITKPKEVGALMRAIDDYKGSVVVRAALLFSALT
HHHCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FCRPGEIRHVEWAEINFEREEWTIPAEKMKGRTEHRVPLSRQALEVLHGIMPYTGSAKYV
HCCCCCCCEEEEHEEEECHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEE
FPTPRSNNRPLSENGVLSALRNMGYTKEQMTAHGFRSMASTLLNELGHRPDAIEAQLAHK
ECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
GADKIRATYNRAEYMKERRNLMQAWADYLEDLKMQSMQPGQI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]