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The map label for this gene is dapF

Identifier: 220904420

GI number: 220904420

Start: 1355089

End: 1355952

Strand: Direct

Name: dapF

Synonym: Ddes_1150

Alternate gene names: 220904420

Gene position: 1355089-1355952 (Clockwise)

Preceding gene: 220904419

Following gene: 220904421

Centisome position: 47.16

GC content: 54.51

Gene sequence:

>864_bases
ATGGCTGTGGCATTGCGCTTTACGAAGATGCAGGGCATCGGCAATGACTATGTTTATATCAACGGCTTTCAAGAGCGTAT
TGACAGCCCGGGAGAGCTCGCCAGAAAAATCAGTGATCGGCATTTCGGCATCGGGTCTGACGGGCTTGTGCTTATTTTGC
CATCAGCGACGGCCGATGTGCGCATGCGTATGTTCAATGCTGACGGCTCTGAATCGGAAATGTGCGGCAACGCAGTGCGT
TGCGTGGGAAAATATGTTTATGATCACGGCATACAGGTCAAAGACGTTATCACGGTAGAAACCCGCGCGGGCGTCAAGAT
TGTTCGCCTGCTGTTTGAAGCGGGAAAAGTTTGCGGTGCGACTGTCGATATGGGGGAACCTGAACTGCATCCTGCCAGAA
TTCCTGTACTCACCGAAACCTCCGGAGACGGCAGCCAGCAGCGTTTTGTGGCCCGCCCCGTGGACGTAAACGGTCAACTA
TACGAAATAACAGCTGTATCGATGGGCAATCCTCACGCCGTCATTTTTATGAAGGGTATTGACGACCTTGATCTTCCCCG
TATCGGGCCGCGTTTTGAACATCACCCTTTGTTCCCAAAACGCACCAATACGGAGTTTGCGGAGGTCATCTCTTCCACCA
AGGTGCGCATGCGCGTTTGGGAGCGCGGAGCAGGCGAAACGCTTGCCTGCGGAACCGGAGCCTGCGCCGTTGCCGTTGCC
TGCGTGCTGAACGGGTACGCCGGGCGTGATGTGGAGGTGGAGCTTAAGGGCGGTTCCTTACATATCCACTGGGATGAAGC
CAGTAACCACGTATACATGACCGGCGGCGCGGTGACCGTTTTTTCCGGCGAATACTACATTTGA

Upstream 100 bases:

>100_bases
TTTACTTTTGATTTGAATGGGAATACTTTCTTTATACATCATATTGTCAAAGATGTTTTTTAGGGGCGTTTGAGTGCCCT
TGAAAGAAAAAGGAGTGACT

Downstream 100 bases:

>100_bases
ACCAGAATGAAGAGGATGGCATGGCTACCGTAAACAGCAATTTTCTCAAACTCCAGAGCAATTATCTTTTTGCGGACATC
GCCCGTAAGGTTACGGCTTT

Product: Diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAVALRFTKMQGIGNDYVYINGFQERIDSPGELARKISDRHFGIGSDGLVLILPSATADVRMRMFNADGSESEMCGNAVR
CVGKYVYDHGIQVKDVITVETRAGVKIVRLLFEAGKVCGATVDMGEPELHPARIPVLTETSGDGSQQRFVARPVDVNGQL
YEITAVSMGNPHAVIFMKGIDDLDLPRIGPRFEHHPLFPKRTNTEFAEVISSTKVRMRVWERGAGETLACGTGACAVAVA
CVLNGYAGRDVEVELKGGSLHIHWDEASNHVYMTGGAVTVFSGEYYI

Sequences:

>Translated_287_residues
MAVALRFTKMQGIGNDYVYINGFQERIDSPGELARKISDRHFGIGSDGLVLILPSATADVRMRMFNADGSESEMCGNAVR
CVGKYVYDHGIQVKDVITVETRAGVKIVRLLFEAGKVCGATVDMGEPELHPARIPVLTETSGDGSQQRFVARPVDVNGQL
YEITAVSMGNPHAVIFMKGIDDLDLPRIGPRFEHHPLFPKRTNTEFAEVISSTKVRMRVWERGAGETLACGTGACAVAVA
CVLNGYAGRDVEVELKGGSLHIHWDEASNHVYMTGGAVTVFSGEYYI
>Mature_286_residues
AVALRFTKMQGIGNDYVYINGFQERIDSPGELARKISDRHFGIGSDGLVLILPSATADVRMRMFNADGSESEMCGNAVRC
VGKYVYDHGIQVKDVITVETRAGVKIVRLLFEAGKVCGATVDMGEPELHPARIPVLTETSGDGSQQRFVARPVDVNGQLY
EITAVSMGNPHAVIFMKGIDDLDLPRIGPRFEHHPLFPKRTNTEFAEVISSTKVRMRVWERGAGETLACGTGACAVAVAC
VLNGYAGRDVEVELKGGSLHIHWDEASNHVYMTGGAVTVFSGEYYI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=37.0106761565836, Blast_Score=193, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_DESDA (B8IZX7)

Other databases:

- EMBL:   CP001358
- RefSeq:   YP_002479732.1
- ProteinModelPortal:   B8IZX7
- GeneID:   7284832
- GenomeReviews:   CP001358_GR
- KEGG:   dds:Ddes_1150
- HOGENOM:   HBG399442
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31239; Mature: 31108

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVALRFTKMQGIGNDYVYINGFQERIDSPGELARKISDRHFGIGSDGLVLILPSATADV
CEEEEEEEEECCCCCCEEEECCHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEE
RMRMFNADGSESEMCGNAVRCVGKYVYDHGIQVKDVITVETRAGVKIVRLLFEAGKVCGA
EEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCC
TVDMGEPELHPARIPVLTETSGDGSQQRFVARPVDVNGQLYEITAVSMGNPHAVIFMKGI
EECCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCEEEEEEEEECCCCEEEEEEECC
DDLDLPRIGPRFEHHPLFPKRTNTEFAEVISSTKVRMRVWERGAGETLACGTGACAVAVA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCEEECCCCHHHHHHH
CVLNGYAGRDVEVELKGGSLHIHWDEASNHVYMTGGAVTVFSGEYYI
HHHCCCCCCEEEEEEECCEEEEEEECCCCEEEEECCEEEEECCCCCC
>Mature Secondary Structure 
AVALRFTKMQGIGNDYVYINGFQERIDSPGELARKISDRHFGIGSDGLVLILPSATADV
EEEEEEEEECCCCCCEEEECCHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEE
RMRMFNADGSESEMCGNAVRCVGKYVYDHGIQVKDVITVETRAGVKIVRLLFEAGKVCGA
EEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCC
TVDMGEPELHPARIPVLTETSGDGSQQRFVARPVDVNGQLYEITAVSMGNPHAVIFMKGI
EECCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCEEEEEEEEECCCCEEEEEEECC
DDLDLPRIGPRFEHHPLFPKRTNTEFAEVISSTKVRMRVWERGAGETLACGTGACAVAVA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCEEECCCCHHHHHHH
CVLNGYAGRDVEVELKGGSLHIHWDEASNHVYMTGGAVTVFSGEYYI
HHHCCCCCCEEEEEEECCEEEEEEECCCCEEEEECCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA