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The map label for this gene is vnfA [H]

Identifier: 220904416

GI number: 220904416

Start: 1348605

End: 1350173

Strand: Direct

Name: vnfA [H]

Synonym: Ddes_1146

Alternate gene names: 220904416

Gene position: 1348605-1350173 (Clockwise)

Preceding gene: 220904415

Following gene: 220904417

Centisome position: 46.93

GC content: 53.86

Gene sequence:

>1569_bases
ATGCAGCAGACAGATCCTGTTCATGATAGTGTAAAAAATCATCACGAGCAGCAGCTCAGCCTTTTATATGAGCTTGCGCA
GATCATGAATTTCACGACAGATTTGACTCAGGCTCTGGATAAGGCGTTAAAGCTCATGGCCAGGCAACTGCACATGATGC
GCGGGGCCATTACCCTCATCTCGCCGCATACTGGCGAGATACGAACAGAGGCGGGCTACGGCCTGAAACCTGCTGAACTA
CGGCGCGGCCGTTACGTGCGCGGTGAAGGCATCACGGGCAGAGTTATTGAAAGCGGCCGCCCCATGTACATCTCCAATGT
ATCTGAAGAACCGCTGTTTCTTAACCGTACCCGTTCACGCGATTTGAGCAAGGAAGGCATTTCCTTTATATGTGTGCCCA
TTCGTCTCAATGATCAGGTTGTGGGCGCGCTTTCTGTGGATCATCTGTTAGTGGATGAAGCTACCCTTGAAGATGAAATG
CGCCTTCTGACCATAGTATCCACGCTTCTTGGGCATGCCGCGCTGGAAACTCAGGGGCGCATGGACGAAGAAACGTCCTC
CCCCCTGCGCCCCAGAGGGTTTGTAGGCAATTCCGAGGGGATGCAAAAAGTCTATGCCCAGATAGCCCAGGTGGCTCCCT
CAGTAACAACGGTATTTCTTCAGGGTGAGTCGGGTACGGGTAAAGAGCTTGCCGCCCGTGCCATTCACTCAGGCAGCGCC
CGTGCCGGTAAGCCATTTATTTCTCTCAATTGTGCGGCCTTGCCGGAAAATCTGATAGAGAGCGAACTGTTTGGTCATGA
GCGCGGGGCTTTTACCGGGGCCAGCGCCACGCGCAAGGGACGGTTTGAACTGGCCCACGGTGGCACACTGTTTCTTGATG
AAGTGGGCGAGCTGTCTCTCATGACCCAGGCCAAGCTTTTGCGTGTCTTGCAGGAGCGTGCCTTTGAGCGCCTGGGCGGA
ATGGAAACCCATTATGTGGACGTACGTTTTATCACTGCCACCAACAGGAATCTTGAACACATGGTTGCCAAGGAAAGCTT
CCGGCGCGACCTTTTTTACCGGTTGAACGTGTTTCCCATCTATCTGCCTCCGCTGCGGGGGCGGCCTGAAGATATACTTC
CGCTTGCCAACCATTTTATCAGAAAATTTGCTCAGGCCAATGGACGGGAAAACGTACGTCTTTCTTTGTCTGTGATGGAT
ATGCTGCAACGTTACGCATGGCCGGGCAACATTCGCGAACTGGAAAATGTCATGGAAAGGGCGGTACTGCTTTTGGGCCG
TGAAGGCCTTGTTTTGCCGCAACACCTGCCCCCGGCTTTGCATGGAGCACACTGCGCCGGTATAAGCGGAGAGAGCGCCT
GTGCAGCGCGCCCAGGATTTTCCGGCAGCCTGCAGGATCAGCTGGATGAGCTTGAACGGGCTTCCATAGTCGAAGCTCTG
GAATTCAGCCAGGGCCAAATGGGCAAGGCCGCTGCGAGCCTTGGCCTGACAGAGCGTATCATGGCGTTACGCATGAAAAA
ATATGAGATCAATTATAAGGAATTCCGTCCCAGGCATAACCGTTCATAG

Upstream 100 bases:

>100_bases
TAGTCAGCCCTGTTTCAGGGGCGACAACACATTGCATGCCCGGCGCTGCTGAAGTATACCTTTCAGAGTGAACAACCCTA
TTTTATTCGGGCAAGAAACT

Downstream 100 bases:

>100_bases
CCTGTTGCAGCCCCGTCATTACTTTTGGGGCGGTTTTGCTGCTGCAGATCAAAAAATACCCCGATCCACGGCTCTGTTCA
AAGAGCAAGAGTTGTCTCAG

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MQQTDPVHDSVKNHHEQQLSLLYELAQIMNFTTDLTQALDKALKLMARQLHMMRGAITLISPHTGEIRTEAGYGLKPAEL
RRGRYVRGEGITGRVIESGRPMYISNVSEEPLFLNRTRSRDLSKEGISFICVPIRLNDQVVGALSVDHLLVDEATLEDEM
RLLTIVSTLLGHAALETQGRMDEETSSPLRPRGFVGNSEGMQKVYAQIAQVAPSVTTVFLQGESGTGKELAARAIHSGSA
RAGKPFISLNCAALPENLIESELFGHERGAFTGASATRKGRFELAHGGTLFLDEVGELSLMTQAKLLRVLQERAFERLGG
METHYVDVRFITATNRNLEHMVAKESFRRDLFYRLNVFPIYLPPLRGRPEDILPLANHFIRKFAQANGRENVRLSLSVMD
MLQRYAWPGNIRELENVMERAVLLLGREGLVLPQHLPPALHGAHCAGISGESACAARPGFSGSLQDQLDELERASIVEAL
EFSQGQMGKAAASLGLTERIMALRMKKYEINYKEFRPRHNRS

Sequences:

>Translated_522_residues
MQQTDPVHDSVKNHHEQQLSLLYELAQIMNFTTDLTQALDKALKLMARQLHMMRGAITLISPHTGEIRTEAGYGLKPAEL
RRGRYVRGEGITGRVIESGRPMYISNVSEEPLFLNRTRSRDLSKEGISFICVPIRLNDQVVGALSVDHLLVDEATLEDEM
RLLTIVSTLLGHAALETQGRMDEETSSPLRPRGFVGNSEGMQKVYAQIAQVAPSVTTVFLQGESGTGKELAARAIHSGSA
RAGKPFISLNCAALPENLIESELFGHERGAFTGASATRKGRFELAHGGTLFLDEVGELSLMTQAKLLRVLQERAFERLGG
METHYVDVRFITATNRNLEHMVAKESFRRDLFYRLNVFPIYLPPLRGRPEDILPLANHFIRKFAQANGRENVRLSLSVMD
MLQRYAWPGNIRELENVMERAVLLLGREGLVLPQHLPPALHGAHCAGISGESACAARPGFSGSLQDQLDELERASIVEAL
EFSQGQMGKAAASLGLTERIMALRMKKYEINYKEFRPRHNRS
>Mature_522_residues
MQQTDPVHDSVKNHHEQQLSLLYELAQIMNFTTDLTQALDKALKLMARQLHMMRGAITLISPHTGEIRTEAGYGLKPAEL
RRGRYVRGEGITGRVIESGRPMYISNVSEEPLFLNRTRSRDLSKEGISFICVPIRLNDQVVGALSVDHLLVDEATLEDEM
RLLTIVSTLLGHAALETQGRMDEETSSPLRPRGFVGNSEGMQKVYAQIAQVAPSVTTVFLQGESGTGKELAARAIHSGSA
RAGKPFISLNCAALPENLIESELFGHERGAFTGASATRKGRFELAHGGTLFLDEVGELSLMTQAKLLRVLQERAFERLGG
METHYVDVRFITATNRNLEHMVAKESFRRDLFYRLNVFPIYLPPLRGRPEDILPLANHFIRKFAQANGRENVRLSLSVMD
MLQRYAWPGNIRELENVMERAVLLLGREGLVLPQHLPPALHGAHCAGISGESACAARPGFSGSLQDQLDELERASIVEAL
EFSQGQMGKAAASLGLTERIMALRMKKYEINYKEFRPRHNRS

Specific function: Required for the expression of the V-dependent nitrogen fixation system in Azotobacter vinelandii. It is required for the regulation of nitrogenase 2 transcription. Interacts with sigma- 54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=318, Percent_Identity=48.7421383647799, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1790437, Length=317, Percent_Identity=48.8958990536278, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI87082117, Length=556, Percent_Identity=37.2302158273381, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1789087, Length=329, Percent_Identity=48.3282674772036, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1790299, Length=328, Percent_Identity=44.8170731707317, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=43.167701863354, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87082152, Length=252, Percent_Identity=49.6031746031746, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=40.1869158878505, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1788905, Length=232, Percent_Identity=49.1379310344828, Blast_Score=219, Evalue=5e-58,
Organism=Escherichia coli, GI1786524, Length=267, Percent_Identity=47.940074906367, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI87081872, Length=324, Percent_Identity=38.8888888888889, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=32.475884244373, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1789828, Length=238, Percent_Identity=35.2941176470588, Blast_Score=132, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 58145; Mature: 58145

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQTDPVHDSVKNHHEQQLSLLYELAQIMNFTTDLTQALDKALKLMARQLHMMRGAITLI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
SPHTGEIRTEAGYGLKPAELRRGRYVRGEGITGRVIESGRPMYISNVSEEPLFLNRTRSR
CCCCCCEEECCCCCCCHHHHHCCCEEECCCCEEHEECCCCCEEEECCCCCCEEEECCCCC
DLSKEGISFICVPIRLNDQVVGALSVDHLLVDEATLEDEMRLLTIVSTLLGHAALETQGR
CCHHCCCEEEEEEEEECCCEEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MDEETSSPLRPRGFVGNSEGMQKVYAQIAQVAPSVTTVFLQGESGTGKELAARAIHSGSA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCC
RAGKPFISLNCAALPENLIESELFGHERGAFTGASATRKGRFELAHGGTLFLDEVGELSL
CCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCHHH
MTQAKLLRVLQERAFERLGGMETHYVDVRFITATNRNLEHMVAKESFRRDLFYRLNVFPI
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEE
YLPPLRGRPEDILPLANHFIRKFAQANGRENVRLSLSVMDMLQRYAWPGNIRELENVMER
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHH
AVLLLGREGLVLPQHLPPALHGAHCAGISGESACAARPGFSGSLQDQLDELERASIVEAL
HHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EFSQGQMGKAAASLGLTERIMALRMKKYEINYKEFRPRHNRS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC
>Mature Secondary Structure
MQQTDPVHDSVKNHHEQQLSLLYELAQIMNFTTDLTQALDKALKLMARQLHMMRGAITLI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
SPHTGEIRTEAGYGLKPAELRRGRYVRGEGITGRVIESGRPMYISNVSEEPLFLNRTRSR
CCCCCCEEECCCCCCCHHHHHCCCEEECCCCEEHEECCCCCEEEECCCCCCEEEECCCCC
DLSKEGISFICVPIRLNDQVVGALSVDHLLVDEATLEDEMRLLTIVSTLLGHAALETQGR
CCHHCCCEEEEEEEEECCCEEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MDEETSSPLRPRGFVGNSEGMQKVYAQIAQVAPSVTTVFLQGESGTGKELAARAIHSGSA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCC
RAGKPFISLNCAALPENLIESELFGHERGAFTGASATRKGRFELAHGGTLFLDEVGELSL
CCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCHHH
MTQAKLLRVLQERAFERLGGMETHYVDVRFITATNRNLEHMVAKESFRRDLFYRLNVFPI
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEE
YLPPLRGRPEDILPLANHFIRKFAQANGRENVRLSLSVMDMLQRYAWPGNIRELENVMER
EECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHH
AVLLLGREGLVLPQHLPPALHGAHCAGISGESACAARPGFSGSLQDQLDELERASIVEAL
HHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EFSQGQMGKAAASLGLTERIMALRMKKYEINYKEFRPRHNRS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]