Click here to switch to the map view.

The map label for this gene is nrgA [H]

Identifier: 220904417

GI number: 220904417

Start: 1350932

End: 1352137

Strand: Direct

Name: nrgA [H]

Synonym: Ddes_1147

Alternate gene names: 220904417

Gene position: 1350932-1352137 (Clockwise)

Preceding gene: 220904416

Following gene: 220904418

Centisome position: 47.01

GC content: 58.46

Gene sequence:

>1206_bases
ATGAATGCCCCTGATACTGCCTTTGTCATTATCTGCGCCACCATGGTCATGCTCATGATTCCGGCTTTGGCCCTGTTTTA
TGGTGGCCTGGTCCGCGCCCGCAATGTTCTTTCCACCCATATGTATAGCTACGCCTGCCTTGGGCTTGTTTCCGTCCTTT
GGGCGCTTGTGGGCTACACACTGGCCTTTGGCGACGATATGGGCGGACTTGTGGGCAATTTCAGATTTGCTCTGCTTGAC
GGGGTGAACGGCAATCCCGCCCCTATGGCGCCGCAGCTGCCGCATACGGTTTTTATGGGCTTTCAATGTATGTTTGCCGT
GCTCACGGTAGCCTTGATTTCAGGAGCGTACGCAGAACGTATGCGTTTTTCTGCCATGCTGCTTTTCTCTGCCTTGTGGC
TGGTTCTTGTGTACGCTCCTATGGCTCACTGGGTGTGGGGCGGAGGCTGGATGGGCCGGATGGGCGCACTGGATTTTGCC
GGTGGCGCTGTGGTGCACATGTCTTCGGGAGCCGCCGCGCTTGCCTGCGCCCAAGCCTTGGGTCCCAGGATTTCAACCGG
CTCACGGCATGTTCCCCATAATCTGCCGCTGACGCTTCTCGGTGGCGGTATCCTCTGGTTCGGCTGGTTCGGCTTTAACG
CCGGCAGCGCGCTGGCCGCAGGAACCCTTGCGGGGCAGGCGCTGGTGACAACGCATATGGCGGCGGCATGCAGCATTCTT
GGCTGGATAATTGTTGAATGGAAGCATACGGGCAAGCCTACCAGCCTGGGAGCAATCTCCGGCGCTCTGGCAGGGCTTGT
GGCCATTACCCCGGGCGCTGGTTTTGTGGATGTGCTGCCAGCCATGCTCATAGGCTTTATCGGCGGAATGGTCTGCTACG
GCGGTGTATTGCTGAAGAGCCGGTTCGGTTATGACGATGCCCTTGACGTCGTGGGCATACACGGCCTCGGCGGAACCTGG
GGCGCACTGGCTACAGGTTTGTTCGCCAGCGCGGCAGTCAATGGCGTGGACGGTCTTTTTTACGGAAATCCCGGCCAGGT
CTGGATACAGCTTGCATCCATCGCAGGTACCTGGGCTTTTGTTTATGTTGCCACGCGCATCATTGTCCGTCTGGTGGGCG
CAATGGTCGGATTGCGGGTTGCCCCCGAGGAAGAGTACAGCGGCCTTGACCTCGGCGAACACAATGAGCGCGGTTATAAT
CTGTAA

Upstream 100 bases:

>100_bases
GGTAGGCAACGATATTGTATCGTCGCTGCGGATCCGGCGCCGGTCCGCTGAAGAGATTCAGCAACACGCACACCTTGTGC
GTAATGATCAAGGACGCCCT

Downstream 100 bases:

>100_bases
TCCGGGAGAGACTGTGTCATGAAGAAGCTCGAGATAATTATTCGTCCCGGTATGTTTGAAAAAGTCAGGGATGTGCTCTC
AGAACTGGGCATACACGGAT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNAPDTAFVIICATMVMLMIPALALFYGGLVRARNVLSTHMYSYACLGLVSVLWALVGYTLAFGDDMGGLVGNFRFALLD
GVNGNPAPMAPQLPHTVFMGFQCMFAVLTVALISGAYAERMRFSAMLLFSALWLVLVYAPMAHWVWGGGWMGRMGALDFA
GGAVVHMSSGAAALACAQALGPRISTGSRHVPHNLPLTLLGGGILWFGWFGFNAGSALAAGTLAGQALVTTHMAAACSIL
GWIIVEWKHTGKPTSLGAISGALAGLVAITPGAGFVDVLPAMLIGFIGGMVCYGGVLLKSRFGYDDALDVVGIHGLGGTW
GALATGLFASAAVNGVDGLFYGNPGQVWIQLASIAGTWAFVYVATRIIVRLVGAMVGLRVAPEEEYSGLDLGEHNERGYN
L

Sequences:

>Translated_401_residues
MNAPDTAFVIICATMVMLMIPALALFYGGLVRARNVLSTHMYSYACLGLVSVLWALVGYTLAFGDDMGGLVGNFRFALLD
GVNGNPAPMAPQLPHTVFMGFQCMFAVLTVALISGAYAERMRFSAMLLFSALWLVLVYAPMAHWVWGGGWMGRMGALDFA
GGAVVHMSSGAAALACAQALGPRISTGSRHVPHNLPLTLLGGGILWFGWFGFNAGSALAAGTLAGQALVTTHMAAACSIL
GWIIVEWKHTGKPTSLGAISGALAGLVAITPGAGFVDVLPAMLIGFIGGMVCYGGVLLKSRFGYDDALDVVGIHGLGGTW
GALATGLFASAAVNGVDGLFYGNPGQVWIQLASIAGTWAFVYVATRIIVRLVGAMVGLRVAPEEEYSGLDLGEHNERGYN
L
>Mature_401_residues
MNAPDTAFVIICATMVMLMIPALALFYGGLVRARNVLSTHMYSYACLGLVSVLWALVGYTLAFGDDMGGLVGNFRFALLD
GVNGNPAPMAPQLPHTVFMGFQCMFAVLTVALISGAYAERMRFSAMLLFSALWLVLVYAPMAHWVWGGGWMGRMGALDFA
GGAVVHMSSGAAALACAQALGPRISTGSRHVPHNLPLTLLGGGILWFGWFGFNAGSALAAGTLAGQALVTTHMAAACSIL
GWIIVEWKHTGKPTSLGAISGALAGLVAITPGAGFVDVLPAMLIGFIGGMVCYGGVLLKSRFGYDDALDVVGIHGLGGTW
GALATGLFASAAVNGVDGLFYGNPGQVWIQLASIAGTWAFVYVATRIIVRLVGAMVGLRVAPEEEYSGLDLGEHNERGYN
L

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=405, Percent_Identity=44.4444444444444, Blast_Score=303, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17550182, Length=443, Percent_Identity=30.2483069977427, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=439, Percent_Identity=27.5626423690205, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=319, Percent_Identity=31.6614420062696, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=432, Percent_Identity=25.9259259259259, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324187, Length=405, Percent_Identity=36.7901234567901, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6325396, Length=413, Percent_Identity=31.9612590799031, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321559, Length=413, Percent_Identity=30.7506053268765, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI161078314, Length=421, Percent_Identity=32.3040380047506, Blast_Score=144, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 41814; Mature: 41814

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPDTAFVIICATMVMLMIPALALFYGGLVRARNVLSTHMYSYACLGLVSVLWALVGYT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFGDDMGGLVGNFRFALLDGVNGNPAPMAPQLPHTVFMGFQCMFAVLTVALISGAYAER
HHCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MRFSAMLLFSALWLVLVYAPMAHWVWGGGWMGRMGALDFAGGAVVHMSSGAAALACAQAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEEECCCHHHHHHHHHH
GPRISTGSRHVPHNLPLTLLGGGILWFGWFGFNAGSALAAGTLAGQALVTTHMAAACSIL
CCCCCCCCCCCCCCCCHHEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GWIIVEWKHTGKPTSLGAISGALAGLVAITPGAGFVDVLPAMLIGFIGGMVCYGGVLLKS
HHHHEEEECCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGYDDALDVVGIHGLGGTWGALATGLFASAAVNGVDGLFYGNPGQVWIQLASIAGTWAF
HCCCCCCHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCEEHHHHHHHHHHHH
VYVATRIIVRLVGAMVGLRVAPEEEYSGLDLGEHNERGYNL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAPDTAFVIICATMVMLMIPALALFYGGLVRARNVLSTHMYSYACLGLVSVLWALVGYT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFGDDMGGLVGNFRFALLDGVNGNPAPMAPQLPHTVFMGFQCMFAVLTVALISGAYAER
HHCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MRFSAMLLFSALWLVLVYAPMAHWVWGGGWMGRMGALDFAGGAVVHMSSGAAALACAQAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEEECCCHHHHHHHHHH
GPRISTGSRHVPHNLPLTLLGGGILWFGWFGFNAGSALAAGTLAGQALVTTHMAAACSIL
CCCCCCCCCCCCCCCCHHEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GWIIVEWKHTGKPTSLGAISGALAGLVAITPGAGFVDVLPAMLIGFIGGMVCYGGVLLKS
HHHHEEEECCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGYDDALDVVGIHGLGGTWGALATGLFASAAVNGVDGLFYGNPGQVWIQLASIAGTWAF
HCCCCCCHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCEEHHHHHHHHHHHH
VYVATRIIVRLVGAMVGLRVAPEEEYSGLDLGEHNERGYNL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]