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The map label for this gene is pyk2 [H]

Identifier: 220904374

GI number: 220904374

Start: 1293966

End: 1295384

Strand: Reverse

Name: pyk2 [H]

Synonym: Ddes_1103

Alternate gene names: 220904374

Gene position: 1295384-1293966 (Counterclockwise)

Preceding gene: 220904391

Following gene: 220904373

Centisome position: 45.08

GC content: 57.51

Gene sequence:

>1419_bases
ATGAGAACCAAAATTGTCGCCACCATCGGCCCCGCTTCCAACAGCAGGGAAAAACTGCACGAGCTTGCAGAGGCTGGCGT
AAGCGTTTTCCGTCTCAACTTCTCACATGGCGCTGCTGCCGACTTTGTGAACATCATAAAAAACATCCGGGAAGTAGAGC
AGTCTCTTGGGCGGCCTATCACTATTATGCAGGACCTTTCCGGGCCAAAAATTCGCTTGGGCGTGGTTCCCGAGGGGACG
ATAGAAGTAGAAAAAGGCATGCGCCTGCTGCTTGGCCCAAGCCAGTCACGCGTAGATGAAATGGCCTACCTGCCTTTTGA
TCATGAAGAAATCCTTGAAAGCCTGGAACCCGGCGACCGCCTTGTTCTGGCCGATGGCGGCCTTCAGTTCATCGTGCAGG
CGCGCAGGGCCGACGACCTTGTGCTGCTGGAAGCCGACAACAGCGGCATTGTTACCTCGCGCAAGGGCCTCGCCCTGCCC
GGCAAGGCCACAAAGGTACGCGCCCTGACAGACAAGGACAAAAAAGACCTCGCAGACGGACTCAAGCTTGGCGTGGACGC
AGTGGCGATTTCCTACGTTCAGACGGCCGATGATGTACGCGAAGCCAAGGAGCTGATCGCCGCTGCCGGGCAGAACCTGC
CTGTGGTGGTCAAGCTGGAACGCCAGAGCGCTGTTGACAATCTTGCAGAAATTCTGGCGGAAACAGATGTGGTTATGGTG
GCCCGCGGCGATCTTGGCGTGGAATGCCCCCTGCCCTTGCTGCCTGCCCTGCAAAAACGCATTATCAGCGCCTGCAACAA
GGCCTCAAAGCCTGTTATCGTCGCTACGCAGATGCTGCTTTCTATGGTCAACAGCCCCGCTCCCACGAGAGCGGAAACCA
CAGACGTGGCTAACGCCGTACTGGACGGGGCCGACTGCGTGATGCTTTCTGAAGAAACCGCCATGGGCAATTTCCCCGTA
GACACAGTACGCTATATGAGGCGCATCACCGATGAAGCTGAAAAGCTGCTGCTGCTCAACCACAATCTTGAAGAGCCCGA
TGCCGACAAGGGCATACCGGAGTTTCTGGCTTACTCAGCTTGCCTGCTGGCCAACAAGGCCGGAGCCAAGGCCATCGTGT
CTCACAGCCTTTCCGGCAGTTCCGCGCGCCAGGTTTCCGCCCGACGTCCTCCGCAGCAGATATACGCCCTTACCCCGGAT
CCGGTGACCGTCAAGGCGCTCAACTTTGTCTGGGGCGTCAAGCCGGTTTTCATCGACCGCCCGCAGGAAGAACCCAGCCA
CCTCATCCGGGCCGAACAGTTCATTCATGACAGCGAAGATTTCGTGGCTGATGATTGCGCCGTTATCACGGCCGGCCAGG
TTAAAGGGTCTTCCTCCACCCCCCGGGGTACCAACCTTGTCAAAATTTACTGGAAATAG

Upstream 100 bases:

>100_bases
ATACGTGCGAAAATCAAGATGATTTTTTGTGCTGGTCACTTGCCCTTTTCTTGCGTCCATACGCCGGACCAGCTTCTTGC
CCTCTAAACAAGGACACATC

Downstream 100 bases:

>100_bases
CCCATGGCCGAGCAAGCACGGAAAGCACGAGAGATTCCCCAGAACATCAATGAGGAATATTATCAGATAAGCAGCGAGAT
ACTTTCAAGCTTTCCCAAGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRTKIVATIGPASNSREKLHELAEAGVSVFRLNFSHGAAADFVNIIKNIREVEQSLGRPITIMQDLSGPKIRLGVVPEGT
IEVEKGMRLLLGPSQSRVDEMAYLPFDHEEILESLEPGDRLVLADGGLQFIVQARRADDLVLLEADNSGIVTSRKGLALP
GKATKVRALTDKDKKDLADGLKLGVDAVAISYVQTADDVREAKELIAAAGQNLPVVVKLERQSAVDNLAEILAETDVVMV
ARGDLGVECPLPLLPALQKRIISACNKASKPVIVATQMLLSMVNSPAPTRAETTDVANAVLDGADCVMLSEETAMGNFPV
DTVRYMRRITDEAEKLLLLNHNLEEPDADKGIPEFLAYSACLLANKAGAKAIVSHSLSGSSARQVSARRPPQQIYALTPD
PVTVKALNFVWGVKPVFIDRPQEEPSHLIRAEQFIHDSEDFVADDCAVITAGQVKGSSSTPRGTNLVKIYWK

Sequences:

>Translated_472_residues
MRTKIVATIGPASNSREKLHELAEAGVSVFRLNFSHGAAADFVNIIKNIREVEQSLGRPITIMQDLSGPKIRLGVVPEGT
IEVEKGMRLLLGPSQSRVDEMAYLPFDHEEILESLEPGDRLVLADGGLQFIVQARRADDLVLLEADNSGIVTSRKGLALP
GKATKVRALTDKDKKDLADGLKLGVDAVAISYVQTADDVREAKELIAAAGQNLPVVVKLERQSAVDNLAEILAETDVVMV
ARGDLGVECPLPLLPALQKRIISACNKASKPVIVATQMLLSMVNSPAPTRAETTDVANAVLDGADCVMLSEETAMGNFPV
DTVRYMRRITDEAEKLLLLNHNLEEPDADKGIPEFLAYSACLLANKAGAKAIVSHSLSGSSARQVSARRPPQQIYALTPD
PVTVKALNFVWGVKPVFIDRPQEEPSHLIRAEQFIHDSEDFVADDCAVITAGQVKGSSSTPRGTNLVKIYWK
>Mature_472_residues
MRTKIVATIGPASNSREKLHELAEAGVSVFRLNFSHGAAADFVNIIKNIREVEQSLGRPITIMQDLSGPKIRLGVVPEGT
IEVEKGMRLLLGPSQSRVDEMAYLPFDHEEILESLEPGDRLVLADGGLQFIVQARRADDLVLLEADNSGIVTSRKGLALP
GKATKVRALTDKDKKDLADGLKLGVDAVAISYVQTADDVREAKELIAAAGQNLPVVVKLERQSAVDNLAEILAETDVVMV
ARGDLGVECPLPLLPALQKRIISACNKASKPVIVATQMLLSMVNSPAPTRAETTDVANAVLDGADCVMLSEETAMGNFPV
DTVRYMRRITDEAEKLLLLNHNLEEPDADKGIPEFLAYSACLLANKAGAKAIVSHSLSGSSARQVSARRPPQQIYALTPD
PVTVKALNFVWGVKPVFIDRPQEEPSHLIRAEQFIHDSEDFVADDCAVITAGQVKGSSSTPRGTNLVKIYWK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=438, Percent_Identity=39.9543378995434, Blast_Score=286, Evalue=4e-77,
Organism=Homo sapiens, GI33286420, Length=438, Percent_Identity=39.9543378995434, Blast_Score=286, Evalue=4e-77,
Organism=Homo sapiens, GI33286418, Length=438, Percent_Identity=40.4109589041096, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI10835121, Length=436, Percent_Identity=38.5321100917431, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI32967597, Length=436, Percent_Identity=38.5321100917431, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=39.3728222996516, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=39.3728222996516, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=37.0212765957447, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=37.0212765957447, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=34.7222222222222, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI1788160, Length=473, Percent_Identity=36.1522198731501, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1787965, Length=421, Percent_Identity=37.7672209026128, Blast_Score=249, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17544584, Length=424, Percent_Identity=36.3207547169811, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=37.9668049792531, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=37.9668049792531, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=37.9668049792531, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=37.9668049792531, Blast_Score=266, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6319279, Length=427, Percent_Identity=38.8758782201405, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6324923, Length=430, Percent_Identity=38.1395348837209, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24648964, Length=433, Percent_Identity=39.9538106235566, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI28571814, Length=433, Percent_Identity=39.9538106235566, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=34.6341463414634, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24581235, Length=427, Percent_Identity=33.0210772833724, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24646914, Length=318, Percent_Identity=35.2201257861635, Blast_Score=158, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50986; Mature: 50986

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKIVATIGPASNSREKLHELAEAGVSVFRLNFSHGAAADFVNIIKNIREVEQSLGRPI
CCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCE
TIMQDLSGPKIRLGVVPEGTIEVEKGMRLLLGPSQSRVDEMAYLPFDHEEILESLEPGDR
EEEECCCCCEEEEEECCCCCEECCCCCEEEECCCHHHHHHHEECCCCHHHHHHHCCCCCE
LVLADGGLQFIVQARRADDLVLLEADNSGIVTSRKGLALPGKATKVRALTDKDKKDLADG
EEEECCCCEEHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCHHHHHHH
LKLGVDAVAISYVQTADDVREAKELIAAAGQNLPVVVKLERQSAVDNLAEILAETDVVMV
HHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCEEEE
ARGDLGVECPLPLLPALQKRIISACNKASKPVIVATQMLLSMVNSPAPTRAETTDVANAV
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LDGADCVMLSEETAMGNFPVDTVRYMRRITDEAEKLLLLNHNLEEPDADKGIPEFLAYSA
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHH
CLLANKAGAKAIVSHSLSGSSARQVSARRPPQQIYALTPDPVTVKALNFVWGVKPVFIDR
HHHHCCCCCHHHHHHCCCCCCHHHHHCCCCHHHEEEECCCCEEEEEEHHHHCCCEEEECC
PQEEPSHLIRAEQFIHDSEDFVADDCAVITAGQVKGSSSTPRGTNLVKIYWK
CCCCHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MRTKIVATIGPASNSREKLHELAEAGVSVFRLNFSHGAAADFVNIIKNIREVEQSLGRPI
CCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCE
TIMQDLSGPKIRLGVVPEGTIEVEKGMRLLLGPSQSRVDEMAYLPFDHEEILESLEPGDR
EEEECCCCCEEEEEECCCCCEECCCCCEEEECCCHHHHHHHEECCCCHHHHHHHCCCCCE
LVLADGGLQFIVQARRADDLVLLEADNSGIVTSRKGLALPGKATKVRALTDKDKKDLADG
EEEECCCCEEHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCHHHHHHH
LKLGVDAVAISYVQTADDVREAKELIAAAGQNLPVVVKLERQSAVDNLAEILAETDVVMV
HHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCEEEE
ARGDLGVECPLPLLPALQKRIISACNKASKPVIVATQMLLSMVNSPAPTRAETTDVANAV
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LDGADCVMLSEETAMGNFPVDTVRYMRRITDEAEKLLLLNHNLEEPDADKGIPEFLAYSA
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHH
CLLANKAGAKAIVSHSLSGSSARQVSARRPPQQIYALTPDPVTVKALNFVWGVKPVFIDR
HHHHCCCCCHHHHHHCCCCCCHHHHHCCCCHHHEEEECCCCEEEEEEHHHHCCCEEEECC
PQEEPSHLIRAEQFIHDSEDFVADDCAVITAGQVKGSSSTPRGTNLVKIYWK
CCCCHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]