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The map label for this gene is rpfG [H]

Identifier: 220904327

GI number: 220904327

Start: 1234987

End: 1236066

Strand: Reverse

Name: rpfG [H]

Synonym: Ddes_1054

Alternate gene names: 220904327

Gene position: 1236066-1234987 (Counterclockwise)

Preceding gene: 220904328

Following gene: 220904325

Centisome position: 43.02

GC content: 55.19

Gene sequence:

>1080_bases
GTGAGCGAGGCGGCAGCCCAGCGCAGCCGCATATTACTTGTGGACGACGCCCCGGAAAACTTACGCATCCTGAGCGAAAG
CCTGCGCGAGGACTATACCATCATGTTTGCCAAAAATGGCCCGGACGCCCTGCGCCTGGCCATGGGTAACCCGCCGCCGG
ACCTCATTCTGCTTGATGTCATCATGCCGGGTATGAACGGATACGAAGTCTGCCGCAGGCTCAAGGAAGATCCCGACACG
CGCGACATTCCCATCATGTTCATCACGGCCCAGAATGAGGAAACCGACGAAGCCACCGGCCTATCCCTGGGCGCGCAAGA
CTATATAGCCAAACCATTTCGCGTTTCCCTGGTGCGCAACAGGGTTGCCAATCAATTGAAATACAAGCGTTATCGCGACC
ATCTCAACGACCTGGTGCACGAACGCACACGCCAGTTGGCTCTTACCCAGGAGGCTACCATTCATGCCATGGCAAGCCTT
GCTGAATGGCGAGACACCGAAACGGGTGCGCACATCAAACGGACGCAAAACTATGTGAAAGCACTGGCGCTGCACATGGC
CGGGCAGCCCAAATACCGTGAACAACTGGACGCGGACGTTATTTCCTGGCTTTATCTTTCGGCCCCGCTGCATGATGTGG
GCAAGGTTGCCATTGCCGACACGGTATTGCACAAACCCGGCCCCCTTACCGATGAAGAATATGAAGCCATGAAGACACAC
ACCGTGCATGGCAGGAATGTTCTTGCCGCTGCGGACAAATTTTTGGGCGAAGACTCTTTTTTGAAAATGGCCTGCGACAT
TGCCCATTGCCATCATGAACGGTGGGATGGCCGGGGGTATCCGCAAGGGCTGAAAGAAGAGGGAATTCCGCTTTCAGCAC
GGCTTATGAGTCTTGCCGATGTGTACGACGCCCTGCGCAGCCAGCGCGTTTACAAGCCTGCCATGAGCCACGAAAGCGCC
GCAAAAATTATCCTGGATGGCAGGGGAACCCAGTTTGACCCTGATGTTGTTGATGCCTTTATGGCTATTCAGGAACAATT
CAAGACCATTGCCGAACGCTACAAAGATCTGGAAGGCTGA

Upstream 100 bases:

>100_bases
GGAAAAGCTACGATATTCTCACTGCCGCTGAAAGCCGCATGGTGCAGCGCATGGCTGAGCACCTGACACAGCGCTTGCCG
GGCTTTACCGGGGGCAGGCA

Downstream 100 bases:

>100_bases
CGCCGCAAGATGCGGCGGCTCTCCACTTTATGCGGCAAGCTTCACTGCTGCGTGCACAGGGTGTCTGGAGTATCCCCAAA
AAAGGCCGTAATCGACGGAG

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSEAAAQRSRILLVDDAPENLRILSESLREDYTIMFAKNGPDALRLAMGNPPPDLILLDVIMPGMNGYEVCRRLKEDPDT
RDIPIMFITAQNEETDEATGLSLGAQDYIAKPFRVSLVRNRVANQLKYKRYRDHLNDLVHERTRQLALTQEATIHAMASL
AEWRDTETGAHIKRTQNYVKALALHMAGQPKYREQLDADVISWLYLSAPLHDVGKVAIADTVLHKPGPLTDEEYEAMKTH
TVHGRNVLAAADKFLGEDSFLKMACDIAHCHHERWDGRGYPQGLKEEGIPLSARLMSLADVYDALRSQRVYKPAMSHESA
AKIILDGRGTQFDPDVVDAFMAIQEQFKTIAERYKDLEG

Sequences:

>Translated_359_residues
MSEAAAQRSRILLVDDAPENLRILSESLREDYTIMFAKNGPDALRLAMGNPPPDLILLDVIMPGMNGYEVCRRLKEDPDT
RDIPIMFITAQNEETDEATGLSLGAQDYIAKPFRVSLVRNRVANQLKYKRYRDHLNDLVHERTRQLALTQEATIHAMASL
AEWRDTETGAHIKRTQNYVKALALHMAGQPKYREQLDADVISWLYLSAPLHDVGKVAIADTVLHKPGPLTDEEYEAMKTH
TVHGRNVLAAADKFLGEDSFLKMACDIAHCHHERWDGRGYPQGLKEEGIPLSARLMSLADVYDALRSQRVYKPAMSHESA
AKIILDGRGTQFDPDVVDAFMAIQEQFKTIAERYKDLEG
>Mature_358_residues
SEAAAQRSRILLVDDAPENLRILSESLREDYTIMFAKNGPDALRLAMGNPPPDLILLDVIMPGMNGYEVCRRLKEDPDTR
DIPIMFITAQNEETDEATGLSLGAQDYIAKPFRVSLVRNRVANQLKYKRYRDHLNDLVHERTRQLALTQEATIHAMASLA
EWRDTETGAHIKRTQNYVKALALHMAGQPKYREQLDADVISWLYLSAPLHDVGKVAIADTVLHKPGPLTDEEYEAMKTHT
VHGRNVLAAADKFLGEDSFLKMACDIAHCHHERWDGRGYPQGLKEEGIPLSARLMSLADVYDALRSQRVYKPAMSHESAA
KIILDGRGTQFDPDVVDAFMAIQEQFKTIAERYKDLEG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=119, Percent_Identity=39.4957983193277, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=39.8230088495575, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082012, Length=119, Percent_Identity=34.453781512605, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789809, Length=79, Percent_Identity=41.7721518987342, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=75, Percent_Identity=42.6666666666667, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=32.7731092436975, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787229, Length=117, Percent_Identity=35.042735042735, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 40495; Mature: 40363

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAAAQRSRILLVDDAPENLRILSESLREDYTIMFAKNGPDALRLAMGNPPPDLILLDV
CCCCHHHCCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCEEHHH
IMPGMNGYEVCRRLKEDPDTRDIPIMFITAQNEETDEATGLSLGAQDYIAKPFRVSLVRN
HHCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHCCCCCCCHHHHCCHHHHHHHHH
RVANQLKYKRYRDHLNDLVHERTRQLALTQEATIHAMASLAEWRDTETGAHIKRTQNYVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ALALHMAGQPKYREQLDADVISWLYLSAPLHDVGKVAIADTVLHKPGPLTDEEYEAMKTH
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
TVHGRNVLAAADKFLGEDSFLKMACDIAHCHHERWDGRGYPQGLKEEGIPLSARLMSLAD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHH
VYDALRSQRVYKPAMSHESAAKIILDGRGTQFDPDVVDAFMAIQEQFKTIAERYKDLEG
HHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEAAAQRSRILLVDDAPENLRILSESLREDYTIMFAKNGPDALRLAMGNPPPDLILLDV
CCCHHHCCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCEEHHH
IMPGMNGYEVCRRLKEDPDTRDIPIMFITAQNEETDEATGLSLGAQDYIAKPFRVSLVRN
HHCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHCCCCCCCHHHHCCHHHHHHHHH
RVANQLKYKRYRDHLNDLVHERTRQLALTQEATIHAMASLAEWRDTETGAHIKRTQNYVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ALALHMAGQPKYREQLDADVISWLYLSAPLHDVGKVAIADTVLHKPGPLTDEEYEAMKTH
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
TVHGRNVLAAADKFLGEDSFLKMACDIAHCHHERWDGRGYPQGLKEEGIPLSARLMSLAD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHHH
VYDALRSQRVYKPAMSHESAAKIILDGRGTQFDPDVVDAFMAIQEQFKTIAERYKDLEG
HHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]