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The map label for this gene is fabI1 [H]

Identifier: 220904321

GI number: 220904321

Start: 1228920

End: 1229690

Strand: Reverse

Name: fabI1 [H]

Synonym: Ddes_1048

Alternate gene names: 220904321

Gene position: 1229690-1228920 (Counterclockwise)

Preceding gene: 220904323

Following gene: 220904320

Centisome position: 42.8

GC content: 56.68

Gene sequence:

>771_bases
ATGCTGCTGCAAGGAAAGAAAGCTCTTATAATGGGATTGGCCAACAACAGAAGCATCGCTTACGGCATTGCCGCTGCCCT
GAAGGCTCAGGGAGCGCGCTTGGCGTTCAACTATGTGGGTGATGCCATTAAAAAACGTGTTGAGCCTCTGAGCGAAGAGC
TGGGCGGCGAGTTTACCTTCCAGTGCGATGTGTGTGACGATGCACAGATTGAAAGTGCCGCATCTATCGTCAAGGAAAAA
TGGGGGGATCTGGACATTCTGGTTCACTCCGTGGCTTTTGCCAATCGCGATGACCTGTGTGGCCGCTTTGTGGACACTTC
GCGCGACGGATTCAATCTTGCCCTGGAAGTTTCGGCCTATTCCCTCACCGGGCTTTGCCGCGCCTTTGAGCCGTTGTTCA
ACGAAAACTCCTCGGTGCTCACCATGACCTACCACGGGTCCACTCAGGTCATTCCCGGCTACAATATCATGGGTGTGGCC
AAGGCCGCTCTTGAAGCGTCCATGCGCTATCTGGCCTACGACCTTGGCCCCAAGGGAGTACGGGTCAACGCCATCAGCGC
CGGCCCCATAAAAACTCTGGCCGCCTCGGCCGTGTCGAGTCTCAAGGATATTTTCACCATGGTGGAAGGCAACTCCCCCC
TGCGCCGCAACGTAACCACAGCCGATGTAGGTGGCGTGGCGGCTTTTCTGGCGTCTGATCTTGCCAGCGCCGTGACAGGG
CAGGTCATTTACGTTGACAGCGGCTTCAGCAAGGTGGGCGTAACCGCCTGA

Upstream 100 bases:

>100_bases
GAATATTTTTTTCATACAGCCTCCAGCCGCAAAAGCGGACAGGCGGCGCAAATTACAACCGCACGCGGCCTGTCCGGCCC
GTATTTTTCAAGGAGGATCC

Downstream 100 bases:

>100_bases
GGATAAAACTATGGGCTACCTGACGCTCAATGCCATAGCGATCACCCTGTTATCCGCACTGGCGCTATGGATTTTTTACC
GCTCGGCCAGGGTCGAAAAA

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLLQGKKALIMGLANNRSIAYGIAAALKAQGARLAFNYVGDAIKKRVEPLSEELGGEFTFQCDVCDDAQIESAASIVKEK
WGDLDILVHSVAFANRDDLCGRFVDTSRDGFNLALEVSAYSLTGLCRAFEPLFNENSSVLTMTYHGSTQVIPGYNIMGVA
KAALEASMRYLAYDLGPKGVRVNAISAGPIKTLAASAVSSLKDIFTMVEGNSPLRRNVTTADVGGVAAFLASDLASAVTG
QVIYVDSGFSKVGVTA

Sequences:

>Translated_256_residues
MLLQGKKALIMGLANNRSIAYGIAAALKAQGARLAFNYVGDAIKKRVEPLSEELGGEFTFQCDVCDDAQIESAASIVKEK
WGDLDILVHSVAFANRDDLCGRFVDTSRDGFNLALEVSAYSLTGLCRAFEPLFNENSSVLTMTYHGSTQVIPGYNIMGVA
KAALEASMRYLAYDLGPKGVRVNAISAGPIKTLAASAVSSLKDIFTMVEGNSPLRRNVTTADVGGVAAFLASDLASAVTG
QVIYVDSGFSKVGVTA
>Mature_256_residues
MLLQGKKALIMGLANNRSIAYGIAAALKAQGARLAFNYVGDAIKKRVEPLSEELGGEFTFQCDVCDDAQIESAASIVKEK
WGDLDILVHSVAFANRDDLCGRFVDTSRDGFNLALEVSAYSLTGLCRAFEPLFNENSSVLTMTYHGSTQVIPGYNIMGVA
KAALEASMRYLAYDLGPKGVRVNAISAGPIKTLAASAVSSLKDIFTMVEGNSPLRRNVTTADVGGVAAFLASDLASAVTG
QVIYVDSGFSKVGVTA

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=250, Percent_Identity=46.4, Blast_Score=243, Evalue=6e-66,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=29.6153846153846, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=28.8, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI2367365, Length=250, Percent_Identity=27.6, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787335, Length=256, Percent_Identity=28.125, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789378, Length=243, Percent_Identity=25.5144032921811, Blast_Score=64, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=196, Percent_Identity=27.5510204081633, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27034; Mature: 27034

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLQGKKALIMGLANNRSIAYGIAAALKAQGARLAFNYVGDAIKKRVEPLSEELGGEFTF
CCCCCCEEEEEEECCCCEEHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCEEEE
QCDVCDDAQIESAASIVKEKWGDLDILVHSVAFANRDDLCGRFVDTSRDGFNLALEVSAY
EEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEHH
SLTGLCRAFEPLFNENSSVLTMTYHGSTQVIPGYNIMGVAKAALEASMRYLAYDLGPKGV
HHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHEECCCCCE
RVNAISAGPIKTLAASAVSSLKDIFTMVEGNSPLRRNVTTADVGGVAAFLASDLASAVTG
EEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
QVIYVDSGFSKVGVTA
CEEEEECCCCCCCCCC
>Mature Secondary Structure
MLLQGKKALIMGLANNRSIAYGIAAALKAQGARLAFNYVGDAIKKRVEPLSEELGGEFTF
CCCCCCEEEEEEECCCCEEHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCEEEE
QCDVCDDAQIESAASIVKEKWGDLDILVHSVAFANRDDLCGRFVDTSRDGFNLALEVSAY
EEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEHH
SLTGLCRAFEPLFNENSSVLTMTYHGSTQVIPGYNIMGVAKAALEASMRYLAYDLGPKGV
HHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHEECCCCCE
RVNAISAGPIKTLAASAVSSLKDIFTMVEGNSPLRRNVTTADVGGVAAFLASDLASAVTG
EEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
QVIYVDSGFSKVGVTA
CEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]