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The map label for this gene is nikA [H]

Identifier: 220904305

GI number: 220904305

Start: 1207870

End: 1209504

Strand: Reverse

Name: nikA [H]

Synonym: Ddes_1032

Alternate gene names: 220904305

Gene position: 1209504-1207870 (Counterclockwise)

Preceding gene: 220904310

Following gene: 220904304

Centisome position: 42.09

GC content: 55.54

Gene sequence:

>1635_bases
ATGAACAGAATTTTCAAGGCGTTTCCCCTATCGTTCCGTCATGCCTGCGGCTGTTTTCTGGCTCTGTTTTTCCTTCTGTT
TTCACTCCTCCCCGCATCTGCTGCCAATGGATGGGACAAAGACACGCTGGTGTACGCCTGGAGCAGCAATGTGGGTCCCC
TTAACCCACACGCCTACGGCGCCAACAAGATGTTCGCCCAGGCCCTCATATATGAGCCGCTGGTGCAATATGCCGAAGGC
GGCAAGATCGTCCCCTGGCTGGCCGAAAGCTGGACAATCTCGCCCGATGGCAAGGTCTACACTTTCAATCTGCGCAAAGA
TGTAAAATTTTCAGACGGCACTCCCTTTACAGCCGATGCAGTGGTAAAAAACTTCGATGCCGTCCACATGAACATCAAGC
GTCATGACTGGCTGGCCACGGCTGACCTTATCGATTCATACAAGGCTGTCGGGCCTCATACCTTTGAACTGGTACTCAAG
CAGCCGTATGCTGCAGCGCTTCAGGAACTGAGCCTGGTGCGCCCCCTGCGCTTTCTCTCCCCCGCGGCCATGCCCGAAGA
CGGCAATACCGCCCAAAGCATAAAAGCACCTGTGGGAACTGGTCCCTGGGTTCTGGCCGAAAGCCGCAAAGGCGAATTTG
ACCGCTTTGTGCGCAACGATAATTACTGGGGCGAAAAACCGCAGATGCGCCAGCTTGTGGTCAAGGTGATTCCTGATGCA
GAAACCCGGGCTGTCGCGCTTGAAAGCGGTGAGGTGGACCTTATAGCCACTGCCATTGCCGACCACGGCTCGGCCGGAGT
AAATCCGGATGCTTACGCCGCCCTGTCCAAGGATCCGGCCTACGCCACCCGCGAATCTTCTCCGCGCAATACCCGTCTGC
TGGCTATGAATACCGCCCTGTTCCCGACCAATGAGCTTGCTGTGCGTCAGGCCATTGTGCGCGCCATTGATCGTCCTTCC
ATAATAAAGGGTGTGCTGCTCGGCCAGGAAGCTCCGGCCGAAGAACTGCTGGCTAAGGACATTCCCTTCTGTGATGTCGC
GCTGAAGCCTTATCCCCATTCTCTTGAAGAGGCCGCCGCCATTCTGGACAAGGCTGGCTGGAAGCTTAAATCCGGCGAAA
AATTCCGGACCAGAGATGGCAAAACCCTGCGCCTTGAACTTAAGTTCATTGCCAACGAAAGCATCATGCGCTCCATTGCC
GAAGTGGTGCAAAGCAACCTGGCCCGCATCGGCGTGGATGTTAAGCTGATGGGCGAAGAGCCTAACGCGTTCGGGGCGTC
GCAGACCGACGGCGCATTCAACATGATATTCTGCGAATCCAACGGTGCGCCCTATGATCCGTTCTCATACGTTGCAGCCA
TGCGCGTGGCCGGACATGCGGACTACCAGGCCCAGCGCGGGCTGCCGCAGAAAAAAGAACTGGATGCAGCCATTACAAAA
GCCCTTGCCACAACGGACACCAAGGAACTTCAGAACCTGTTCACCAGCATTCTGACCATGCTGCATGAAGAAGCCGTTTA
CGTTCCCGTAAGCCGTACTATTGACAAGGCACTGTTCAAAAAGAAAAACATCAAGGATTTCGCCTTCAGCCCTGTCTCCT
ACGAACTGCCTCTGGCGCGGATTTCAAGGCCATAG

Upstream 100 bases:

>100_bases
AAGCATGCACAACGTGTGTCACGTTTTTTTAAAAGTTGACACAAAAATAACATGCCGATACTATTCTAAAAATCCTCACA
ACCCCATACAGGACAAGAAG

Downstream 100 bases:

>100_bases
GTAACTGCCGGCACGACGATCTCCCGTGATCGCCTCTATACCCTGTTGCCCATGCCGTGGTATGCCTCGCGCATTCCACG
GCATGGGCAAAGTGCGACAT

Product: nickel ABC transporter periplasmic nickel-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MNRIFKAFPLSFRHACGCFLALFFLLFSLLPASAANGWDKDTLVYAWSSNVGPLNPHAYGANKMFAQALIYEPLVQYAEG
GKIVPWLAESWTISPDGKVYTFNLRKDVKFSDGTPFTADAVVKNFDAVHMNIKRHDWLATADLIDSYKAVGPHTFELVLK
QPYAAALQELSLVRPLRFLSPAAMPEDGNTAQSIKAPVGTGPWVLAESRKGEFDRFVRNDNYWGEKPQMRQLVVKVIPDA
ETRAVALESGEVDLIATAIADHGSAGVNPDAYAALSKDPAYATRESSPRNTRLLAMNTALFPTNELAVRQAIVRAIDRPS
IIKGVLLGQEAPAEELLAKDIPFCDVALKPYPHSLEEAAAILDKAGWKLKSGEKFRTRDGKTLRLELKFIANESIMRSIA
EVVQSNLARIGVDVKLMGEEPNAFGASQTDGAFNMIFCESNGAPYDPFSYVAAMRVAGHADYQAQRGLPQKKELDAAITK
ALATTDTKELQNLFTSILTMLHEEAVYVPVSRTIDKALFKKKNIKDFAFSPVSYELPLARISRP

Sequences:

>Translated_544_residues
MNRIFKAFPLSFRHACGCFLALFFLLFSLLPASAANGWDKDTLVYAWSSNVGPLNPHAYGANKMFAQALIYEPLVQYAEG
GKIVPWLAESWTISPDGKVYTFNLRKDVKFSDGTPFTADAVVKNFDAVHMNIKRHDWLATADLIDSYKAVGPHTFELVLK
QPYAAALQELSLVRPLRFLSPAAMPEDGNTAQSIKAPVGTGPWVLAESRKGEFDRFVRNDNYWGEKPQMRQLVVKVIPDA
ETRAVALESGEVDLIATAIADHGSAGVNPDAYAALSKDPAYATRESSPRNTRLLAMNTALFPTNELAVRQAIVRAIDRPS
IIKGVLLGQEAPAEELLAKDIPFCDVALKPYPHSLEEAAAILDKAGWKLKSGEKFRTRDGKTLRLELKFIANESIMRSIA
EVVQSNLARIGVDVKLMGEEPNAFGASQTDGAFNMIFCESNGAPYDPFSYVAAMRVAGHADYQAQRGLPQKKELDAAITK
ALATTDTKELQNLFTSILTMLHEEAVYVPVSRTIDKALFKKKNIKDFAFSPVSYELPLARISRP
>Mature_544_residues
MNRIFKAFPLSFRHACGCFLALFFLLFSLLPASAANGWDKDTLVYAWSSNVGPLNPHAYGANKMFAQALIYEPLVQYAEG
GKIVPWLAESWTISPDGKVYTFNLRKDVKFSDGTPFTADAVVKNFDAVHMNIKRHDWLATADLIDSYKAVGPHTFELVLK
QPYAAALQELSLVRPLRFLSPAAMPEDGNTAQSIKAPVGTGPWVLAESRKGEFDRFVRNDNYWGEKPQMRQLVVKVIPDA
ETRAVALESGEVDLIATAIADHGSAGVNPDAYAALSKDPAYATRESSPRNTRLLAMNTALFPTNELAVRQAIVRAIDRPS
IIKGVLLGQEAPAEELLAKDIPFCDVALKPYPHSLEEAAAILDKAGWKLKSGEKFRTRDGKTLRLELKFIANESIMRSIA
EVVQSNLARIGVDVKLMGEEPNAFGASQTDGAFNMIFCESNGAPYDPFSYVAAMRVAGHADYQAQRGLPQKKELDAAITK
ALATTDTKELQNLFTSILTMLHEEAVYVPVSRTIDKALFKKKNIKDFAFSPVSYELPLARISRP

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=519, Percent_Identity=45.4720616570328, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1789966, Length=536, Percent_Identity=25.5597014925373, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787052, Length=498, Percent_Identity=25.7028112449799, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1787762, Length=473, Percent_Identity=26.215644820296, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787551, Length=536, Percent_Identity=24.2537313432836, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1787495, Length=517, Percent_Identity=24.9516441005803, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081878, Length=500, Percent_Identity=24.2, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789397, Length=497, Percent_Identity=23.943661971831, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59946; Mature: 59946

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIFKAFPLSFRHACGCFLALFFLLFSLLPASAANGWDKDTLVYAWSSNVGPLNPHAYG
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
ANKMFAQALIYEPLVQYAEGGKIVPWLAESWTISPDGKVYTFNLRKDVKFSDGTPFTADA
CHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCEEEEEECCCCCEECCCCCCCHHH
VVKNFDAVHMNIKRHDWLATADLIDSYKAVGPHTFELVLKQPYAAALQELSLVRPLRFLS
HHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHC
PAAMPEDGNTAQSIKAPVGTGPWVLAESRKGEFDRFVRNDNYWGEKPQMRQLVVKVIPDA
CCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCC
ETRAVALESGEVDLIATAIADHGSAGVNPDAYAALSKDPAYATRESSPRNTRLLAMNTAL
CCEEEEEECCCEEEEEEEHHCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCEEEEEEEEC
FPTNELAVRQAIVRAIDRPSIIKGVLLGQEAPAEELLAKDIPFCDVALKPYPHSLEEAAA
CCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHH
ILDKAGWKLKSGEKFRTRDGKTLRLELKFIANESIMRSIAEVVQSNLARIGVDVKLMGEE
HHHHCCCCCCCCCCEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCC
PNAFGASQTDGAFNMIFCESNGAPYDPFSYVAAMRVAGHADYQAQRGLPQKKELDAAITK
CCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHH
ALATTDTKELQNLFTSILTMLHEEAVYVPVSRTIDKALFKKKNIKDFAFSPVSYELPLAR
HHHCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCHHCCCCCCEECCHHH
ISRP
CCCC
>Mature Secondary Structure
MNRIFKAFPLSFRHACGCFLALFFLLFSLLPASAANGWDKDTLVYAWSSNVGPLNPHAYG
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
ANKMFAQALIYEPLVQYAEGGKIVPWLAESWTISPDGKVYTFNLRKDVKFSDGTPFTADA
CHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCEEEEEECCCCCEECCCCCCCHHH
VVKNFDAVHMNIKRHDWLATADLIDSYKAVGPHTFELVLKQPYAAALQELSLVRPLRFLS
HHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHC
PAAMPEDGNTAQSIKAPVGTGPWVLAESRKGEFDRFVRNDNYWGEKPQMRQLVVKVIPDA
CCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCC
ETRAVALESGEVDLIATAIADHGSAGVNPDAYAALSKDPAYATRESSPRNTRLLAMNTAL
CCEEEEEECCCEEEEEEEHHCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCEEEEEEEEC
FPTNELAVRQAIVRAIDRPSIIKGVLLGQEAPAEELLAKDIPFCDVALKPYPHSLEEAAA
CCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHH
ILDKAGWKLKSGEKFRTRDGKTLRLELKFIANESIMRSIAEVVQSNLARIGVDVKLMGEE
HHHHCCCCCCCCCCEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCC
PNAFGASQTDGAFNMIFCESNGAPYDPFSYVAAMRVAGHADYQAQRGLPQKKELDAAITK
CCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHH
ALATTDTKELQNLFTSILTMLHEEAVYVPVSRTIDKALFKKKNIKDFAFSPVSYELPLAR
HHHCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCHHCCCCCCEECCHHH
ISRP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]