Click here to switch to the map view.

The map label for this gene is nikB [H]

Identifier: 220904304

GI number: 220904304

Start: 1206783

End: 1207721

Strand: Reverse

Name: nikB [H]

Synonym: Ddes_1031

Alternate gene names: 220904304

Gene position: 1207721-1206783 (Counterclockwise)

Preceding gene: 220904305

Following gene: 220904303

Centisome position: 42.03

GC content: 54.42

Gene sequence:

>939_bases
ATGATACGCTTTTTGTGCCGCCGCCTTCTTGTCCTGTCCCCTCTGCTGCTGGGAGTTTCTGTTTTTGTTTTTGCCGTCCT
GCGCCACGGTAAAACAGATCCGGCAATGGCATATCTGCGTTTGTCCGGTCTGCCGCCCACTGAAAGCCTGCTGGCGACCA
CCCGCGAAAGCCTGGGCCTGAACCAGCCGCTCTGGAAGCAGTATCTGGACTGGCTGAGCTCGGCCCTGCAAGGCGACCTG
GGCCTGTCCTATGTCACAGGCAATCCGGCCCTCAACGAAATCCTGTATTATCTTCCCGCTACCATTGAACTGACCATGTG
GGCTATGGCCGCCACAATTTTCATCAGCGTTCCCCTTGGTATCGCGGCTGCTCTGTACAAAGACCGCTGGCCGGATCACC
TTACCCGTATTTTTGCGTTCAGTGCCGTATCCATGCCCTCATTCTGGCTGGGTTTTCTACTGGTGCTCCTTTTTTCGCGC
CATCTTGACATTCTGCCAGCCATGGGCCGGGGCGGCCCGGAATTTCTGATTTTGCCCGTGTTCACGCTTTCTTTCATGTC
TATGGGGATCAACATACGCCTGGTGCGAGCCAGCATGCTGGAACACATGCATAGCCGCAGCGTCATCTATGCCCGCTCAA
GAGGTTTGAAAAAATGGCGCATCATAGGCCAGCATGTGCTGCGCAATGCTTTTATTCCCGTCCTGACATCCATGAGCATG
CACTTCGGCGAACTGCTCGGCGGGGCTGTGATTGTTGAAAACATTTTCAACTGGCCCGGTGTTGGCCGTTATGTGGTTTC
TGCCATCTACAATCACGACTACCCCGTGATCCAGACGTTTACACTGGTAACCACCCTTATTTTTGTGTTTGTCAACCTGC
TTACTGATGTACTTTACGCGCGCATCGACCCGCGCATCCGCCTTGGAGAAGGACGATGA

Upstream 100 bases:

>100_bases
CCCATGCCGTGGTATGCCTCGCGCATTCCACGGCATGGGCAAAGTGCGACATGGCGTCTGACCGGCGCCGCTCCCTGCAA
CCTCAACGCGGACCCCGTTC

Downstream 100 bases:

>100_bases
ACAAAAAAACGCTCGTAGCCCTTCTCATGCTGCTGCCGCTGATGGCCTGCGCCCTGCTGGGTCCCTGGCTGGCGCCCCAC
GACCCCGAAGCCGTGGAGCT

Product: binding-protein-dependent transport systems inner membrane component

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MIRFLCRRLLVLSPLLLGVSVFVFAVLRHGKTDPAMAYLRLSGLPPTESLLATTRESLGLNQPLWKQYLDWLSSALQGDL
GLSYVTGNPALNEILYYLPATIELTMWAMAATIFISVPLGIAAALYKDRWPDHLTRIFAFSAVSMPSFWLGFLLVLLFSR
HLDILPAMGRGGPEFLILPVFTLSFMSMGINIRLVRASMLEHMHSRSVIYARSRGLKKWRIIGQHVLRNAFIPVLTSMSM
HFGELLGGAVIVENIFNWPGVGRYVVSAIYNHDYPVIQTFTLVTTLIFVFVNLLTDVLYARIDPRIRLGEGR

Sequences:

>Translated_312_residues
MIRFLCRRLLVLSPLLLGVSVFVFAVLRHGKTDPAMAYLRLSGLPPTESLLATTRESLGLNQPLWKQYLDWLSSALQGDL
GLSYVTGNPALNEILYYLPATIELTMWAMAATIFISVPLGIAAALYKDRWPDHLTRIFAFSAVSMPSFWLGFLLVLLFSR
HLDILPAMGRGGPEFLILPVFTLSFMSMGINIRLVRASMLEHMHSRSVIYARSRGLKKWRIIGQHVLRNAFIPVLTSMSM
HFGELLGGAVIVENIFNWPGVGRYVVSAIYNHDYPVIQTFTLVTTLIFVFVNLLTDVLYARIDPRIRLGEGR
>Mature_312_residues
MIRFLCRRLLVLSPLLLGVSVFVFAVLRHGKTDPAMAYLRLSGLPPTESLLATTRESLGLNQPLWKQYLDWLSSALQGDL
GLSYVTGNPALNEILYYLPATIELTMWAMAATIFISVPLGIAAALYKDRWPDHLTRIFAFSAVSMPSFWLGFLLVLLFSR
HLDILPAMGRGGPEFLILPVFTLSFMSMGINIRLVRASMLEHMHSRSVIYARSRGLKKWRIIGQHVLRNAFIPVLTSMSM
HFGELLGGAVIVENIFNWPGVGRYVVSAIYNHDYPVIQTFTLVTTLIFVFVNLLTDVLYARIDPRIRLGEGR

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=311, Percent_Identity=53.6977491961415, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1787053, Length=308, Percent_Identity=40.2597402597403, Blast_Score=223, Evalue=9e-60,
Organism=Escherichia coli, GI1789965, Length=338, Percent_Identity=32.8402366863905, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1787761, Length=337, Percent_Identity=37.6854599406528, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1787497, Length=310, Percent_Identity=31.6129032258064, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787550, Length=272, Percent_Identity=30.8823529411765, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1788504, Length=288, Percent_Identity=26.7361111111111, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35126; Mature: 35126

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFLCRRLLVLSPLLLGVSVFVFAVLRHGKTDPAMAYLRLSGLPPTESLLATTRESLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEECCCCCHHHHHHHHHHHCCC
NQPLWKQYLDWLSSALQGDLGLSYVTGNPALNEILYYLPATIELTMWAMAATIFISVPLG
CCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IAAALYKDRWPDHLTRIFAFSAVSMPSFWLGFLLVLLFSRHLDILPAMGRGGPEFLILPV
HHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHH
FTLSFMSMGINIRLVRASMLEHMHSRSVIYARSRGLKKWRIIGQHVLRNAFIPVLTSMSM
HHHHHHHCCCCEEEHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
HFGELLGGAVIVENIFNWPGVGRYVVSAIYNHDYPVIQTFTLVTTLIFVFVNLLTDVLYA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RIDPRIRLGEGR
HCCCCEECCCCC
>Mature Secondary Structure
MIRFLCRRLLVLSPLLLGVSVFVFAVLRHGKTDPAMAYLRLSGLPPTESLLATTRESLGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEECCCCCHHHHHHHHHHHCCC
NQPLWKQYLDWLSSALQGDLGLSYVTGNPALNEILYYLPATIELTMWAMAATIFISVPLG
CCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IAAALYKDRWPDHLTRIFAFSAVSMPSFWLGFLLVLLFSRHLDILPAMGRGGPEFLILPV
HHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHH
FTLSFMSMGINIRLVRASMLEHMHSRSVIYARSRGLKKWRIIGQHVLRNAFIPVLTSMSM
HHHHHHHCCCCEEEHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
HFGELLGGAVIVENIFNWPGVGRYVVSAIYNHDYPVIQTFTLVTTLIFVFVNLLTDVLYA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RIDPRIRLGEGR
HCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]