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The map label for this gene is spoIVB [H]

Identifier: 218905309

GI number: 218905309

Start: 4002391

End: 4003680

Strand: Reverse

Name: spoIVB [H]

Synonym: BCAH820_4193

Alternate gene names: 218905309

Gene position: 4003680-4002391 (Counterclockwise)

Preceding gene: 218905310

Following gene: 218905308

Centisome position: 75.5

GC content: 36.36

Gene sequence:

>1290_bases
TTGAAATTAGAAAGATTTCGCAAGATAATAGGTATTTGTCTCCTTGTTTCTTTAGTTTTTATTGGGTGTTTTAAACCGCT
TCGGACGTTTATTTCATCCCCGAAACAACTTGTTGTTTTTGAAGGACAACAATCAGAAATAGCTTCATTACCAGTGTTTA
AAGCTTCATCTACAGATCGTCATGTGTTTACAGTAAGTTCAAATGAACAAAAACAAGGACTTATGGTAAATTCTCATCAA
AATGGTGAAGCAGATATGGTGTTTCAGCTTGCTGGTTTTCCGGTGAAAAAAGTAAATGTGAGAGTATTAAAAGATTTCAA
AGTTATTCCAGGTGGACAATCAATCGGTGTAAAGTTAAACACAAAAGGGGTACTTGTTGTAGGCCATCATTTAATTCAAA
CTGAAAAAGGGAAAGTATCTCCTGGTGAAACAGCGGGTGTGCAAATTGGAGATATGATTACTGAAATTAATGGTAAAACA
ATTGAAAGAATGAGTGATGTAGCACCATTTATTCATAATAGTGGGGAAACAGGCGAACCGCTTAATCTCGTTTTGTTAAG
AGATGGAAAGCATATTCGCACTAAGCTAACACCACAAAAAGACAATGGAGAATCTTCTTATCGCATTGGTTTATATATTC
GTGATTCGGCAGCTGGAATCGGAACAATGACATTTGTTCATCCTGATTCTATGAAATATGGTGCGCTTGGTCATGTCATT
TCTGATAACGATACGAAAAAACCGATTCAAGTCGAAGATGGACAAATTATGCGTTCGACAGTTACATCAATTGAAAGAGG
TAGTCACGGAAATCCAGGAGAGAAATTAGCGAGGTTCTCACCAGATCTTGAAGTGATTGGCAATATTACAATAAATAGTC
CATTTGGTATTTTTGGCAAATTGAATACAAATATGACAAATGGCGTAATGGATAAAGCAATGCCTATTGCACTATCTCAT
CAAGTAAAAGAGGGGCCGGCAAAAATATTAACGGTTATTGATCAAGATAAAGTGGAAGCGTTTGATATTGAAGTTGTTAG
TACAGTACCGCAAAAGTTTCCGGCAACAAAAGGCATGGTAATAAAAGTAACAGACAAACGTTTATTAGCGAAAACGGGTG
GTATCGTACAAGGGATGAGCGGAAGTCCAATTGTACAAAACGGGAAAGTAATTGGTGCTGTTACACATGTATTTGTAAAT
GATCCGACATCAGGATATGGTGTTCATATTGAATGGATGTTACATGAAGCTGGAATTAACATTTATGATCAAGAAAGAAA
AGCGAGCTAA

Upstream 100 bases:

>100_bases
TTTCTTAATTCTTCCGGTTATAAATAAAGCATTGCAAGGAGAGATTAAAAAGTGAAGCCAAGAGGCTGAATGTGGGGTAG
GAGCGAGGAGAGTGAACAAA

Downstream 100 bases:

>100_bases
TTAATAATTAGCTCGCTTTTCTATTGAAAAATTCGTTGAAATTTTGTAAAAGCTCCGCAAGAGGTGTTTTTTGTTGTAAA
ATAAAAGGAAAGGGTTGAAC

Product: stage IV sporulation protein B

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKLERFRKIIGICLLVSLVFIGCFKPLRTFISSPKQLVVFEGQQSEIASLPVFKASSTDRHVFTVSSNEQKQGLMVNSHQ
NGEADMVFQLAGFPVKKVNVRVLKDFKVIPGGQSIGVKLNTKGVLVVGHHLIQTEKGKVSPGETAGVQIGDMITEINGKT
IERMSDVAPFIHNSGETGEPLNLVLLRDGKHIRTKLTPQKDNGESSYRIGLYIRDSAAGIGTMTFVHPDSMKYGALGHVI
SDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLARFSPDLEVIGNITINSPFGIFGKLNTNMTNGVMDKAMPIALSH
QVKEGPAKILTVIDQDKVEAFDIEVVSTVPQKFPATKGMVIKVTDKRLLAKTGGIVQGMSGSPIVQNGKVIGAVTHVFVN
DPTSGYGVHIEWMLHEAGINIYDQERKAS

Sequences:

>Translated_429_residues
MKLERFRKIIGICLLVSLVFIGCFKPLRTFISSPKQLVVFEGQQSEIASLPVFKASSTDRHVFTVSSNEQKQGLMVNSHQ
NGEADMVFQLAGFPVKKVNVRVLKDFKVIPGGQSIGVKLNTKGVLVVGHHLIQTEKGKVSPGETAGVQIGDMITEINGKT
IERMSDVAPFIHNSGETGEPLNLVLLRDGKHIRTKLTPQKDNGESSYRIGLYIRDSAAGIGTMTFVHPDSMKYGALGHVI
SDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLARFSPDLEVIGNITINSPFGIFGKLNTNMTNGVMDKAMPIALSH
QVKEGPAKILTVIDQDKVEAFDIEVVSTVPQKFPATKGMVIKVTDKRLLAKTGGIVQGMSGSPIVQNGKVIGAVTHVFVN
DPTSGYGVHIEWMLHEAGINIYDQERKAS
>Mature_429_residues
MKLERFRKIIGICLLVSLVFIGCFKPLRTFISSPKQLVVFEGQQSEIASLPVFKASSTDRHVFTVSSNEQKQGLMVNSHQ
NGEADMVFQLAGFPVKKVNVRVLKDFKVIPGGQSIGVKLNTKGVLVVGHHLIQTEKGKVSPGETAGVQIGDMITEINGKT
IERMSDVAPFIHNSGETGEPLNLVLLRDGKHIRTKLTPQKDNGESSYRIGLYIRDSAAGIGTMTFVHPDSMKYGALGHVI
SDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLARFSPDLEVIGNITINSPFGIFGKLNTNMTNGVMDKAMPIALSH
QVKEGPAKILTVIDQDKVEAFDIEVVSTVPQKFPATKGMVIKVTDKRLLAKTGGIVQGMSGSPIVQNGKVIGAVTHVFVN
DPTSGYGVHIEWMLHEAGINIYDQERKAS

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 46604; Mature: 46604

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLERFRKIIGICLLVSLVFIGCFKPLRTFISSPKQLVVFEGQQSEIASLPVFKASSTDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCEEECCCCCC
HVFTVSSNEQKQGLMVNSHQNGEADMVFQLAGFPVKKVNVRVLKDFKVIPGGQSIGVKLN
EEEEECCCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEEEEEEEEEECCCCCEEEEEEC
TKGVLVVGHHLIQTEKGKVSPGETAGVQIGDMITEINGKTIERMSDVAPFIHNSGETGEP
CCEEEEEEEEEEECCCCCCCCCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
LNLVLLRDGKHIRTKLTPQKDNGESSYRIGLYIRDSAAGIGTMTFVHPDSMKYGALGHVI
EEEEEEECCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCEEE
SDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLARFSPDLEVIGNITINSPFGIFGK
CCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCEEEE
LNTNMTNGVMDKAMPIALSHQVKEGPAKILTVIDQDKVEAFDIEVVSTVPQKFPATKGMV
ECCCCCCCHHHHCCCEEEHHHCCCCCEEEEEEECCCCCEEEEEEHHHHCHHHCCCCCCEE
IKVTDKRLLAKTGGIVQGMSGSPIVQNGKVIGAVTHVFVNDPTSGYGVHIEWMLHEAGIN
EEEECCEEHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCEEEEEEEEEECCCC
IYDQERKAS
EEECCCCCC
>Mature Secondary Structure
MKLERFRKIIGICLLVSLVFIGCFKPLRTFISSPKQLVVFEGQQSEIASLPVFKASSTDR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCEEECCCCCC
HVFTVSSNEQKQGLMVNSHQNGEADMVFQLAGFPVKKVNVRVLKDFKVIPGGQSIGVKLN
EEEEECCCCCCCCEEEECCCCCCCEEEEEECCCCEEEEEEEEEEEEEECCCCCEEEEEEC
TKGVLVVGHHLIQTEKGKVSPGETAGVQIGDMITEINGKTIERMSDVAPFIHNSGETGEP
CCEEEEEEEEEEECCCCCCCCCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
LNLVLLRDGKHIRTKLTPQKDNGESSYRIGLYIRDSAAGIGTMTFVHPDSMKYGALGHVI
EEEEEEECCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCEEE
SDNDTKKPIQVEDGQIMRSTVTSIERGSHGNPGEKLARFSPDLEVIGNITINSPFGIFGK
CCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCEEEE
LNTNMTNGVMDKAMPIALSHQVKEGPAKILTVIDQDKVEAFDIEVVSTVPQKFPATKGMV
ECCCCCCCHHHHCCCEEEHHHCCCCCEEEEEEECCCCCEEEEEEHHHHCHHHCCCCCCEE
IKVTDKRLLAKTGGIVQGMSGSPIVQNGKVIGAVTHVFVNDPTSGYGVHIEWMLHEAGIN
EEEECCEEHHHCCCEEECCCCCCEEECCEEEEEEEEEEECCCCCCCEEEEEEEEEECCCC
IYDQERKAS
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]