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The map label for this gene is recN [H]

Identifier: 218905310

GI number: 218905310

Start: 4003808

End: 4005547

Strand: Reverse

Name: recN [H]

Synonym: BCAH820_4194

Alternate gene names: 218905310

Gene position: 4005547-4003808 (Counterclockwise)

Preceding gene: 218905311

Following gene: 218905309

Centisome position: 75.54

GC content: 36.0

Gene sequence:

>1740_bases
TTGTTATCGGAATTATCGATTAGAAACTTTGCTATTATTGAGGCATTAAATATTTCTTTTCAAAAAGGATTAACGGTTTT
AAGTGGTGAAACAGGGGCCGGAAAATCGATTATTATTGATGCGATTAGTTTACTTGTAGGTGGCCGCGGTTCGGCAGAAT
TTGTTCGATACGGAACAGAAAAAGCTGAGATAGAGGGGCTATTTTATGTGGAAGATGATAAGCATCCATGTATTGAAAAG
GCAGAAGAATTGGATATAGAAATAGAAGACGGCATGATTATTTTGAAGCGTGATATCGCTGCAAACGGAAAAAGCGTATG
TCGTGTAAATGGAAAACTAGTTACCCTTAGTGTATTAAAAGAAATTGGAAAAACACTTGTTGATATTCATGGGCAACACG
AAACGCAAGATTTAATGAATGAAGAACGTCATCTATTTATGCTCGATCATTTTGATGGGGAGCGTATCGTAAAACAATTG
GATATATACCAAAACGTATACGCTGACTATGAGAAGTTAAAAAAACAATTGAAATCGTTAAGTGAAAATGAACAACAAAT
GGCGCATCGCTTAGATTTAATTCAATTCCAGCATGAAGAAATCCGTAAGGCGGATTTAAAGATGGATGAAGAAAATAACT
TAACTGAAGAACGATTGCAAATTTCTAATTTCGAAAAGATTTATAAAGCATTAGGTGACGCATATCGCTCGTTAAGTGCT
GATGGACAAGGGTTAGATAATGTAAGAAGTGCAATGGGACAAATGGAGAGTATTACACATTTAGACGAAGTGTACCAAGA
AAATCATGATTCAWTTGCAAATAGCTATTACCTATTAGAAGAAGTTGCATATCAACTAAGAGAAAAACTCGATATGATGG
AATATGACCCAAATCGTTTAGACGAAATTGAAACGCGTTTAAATGAAATTCGTATGCTAAAGAGAAAATATGGAAATACT
GTAGAAGAGATATTAGCGTATGCTGATAAAATTGAACAAGAGATTTTTACGATTGAAAATAAAGACGTTCATATTGAAAC
GACGAAGAAACAGTTGAAGGAATTAGAAAGTGTTATTCTGAAAGAAGCAACGTTGTTAAGTAATATGCGTCATGAACTTG
CAGAGCATCTTACAAATGCCATTCATCAGGAATTAAAAGAATTGTATATGGAAAAAACAAAATTTGAAGTGAGAATCATA
AAGCGAGAAGGAAATGCGGAAGAGCCTCTCGTGGAGGGAGCACCGGTAAGGCTTACAGCGGACGGTTATGATCATGTGGA
ATTTTATATTTCAACCAATCCAGGTGAGCCGCTAAAACCACTTTCAAAGGTCGCTTCTGGCGGAGAGTTGTCCCGTATTA
TTTTAGCTTTAAAAAGTATTTTTTCTAAGCATCAAGGTGTTGCATCTGTTATTTTTGATGAAGTGGATACTGGTGTAAGT
GGTCGGGTCGCACAGGCTATTGCGGAAAAAATTTATCGAGTATCAGTAAACTCACAAGTACTTTGTATTACGCACTTACC
TCAAGTAGCTTCAATGGCGGATTCGCATTTATTTATCCGAAAACAAGTAGCGAATGATCGAACAATTACATCCGTTACCG
TTTTAACTATGGAGGATAAAGTAACAGAAATAGCTCGAATGATTTCTGGTGTGGAAATTACAGATTTAACGACAGAACAT
GCGAAAGAATTACTTACGCAAGCGCATCATTTTAAACAGACAGCAGAGGCTATCCAGTAA

Upstream 100 bases:

>100_bases
AAAGTAATTTATACATACAAACATATAGTATGACTATTTGAATAAAGAACTGAGAAAAGCCCTTAATAGGGGATTAGTTT
ATAACGAGGTGAATGGGGCA

Downstream 100 bases:

>100_bases
TGGATAGCTTTTTTTCTCAAATGATGATTTCTTAATTCTTCCGGTTATAAATAAAGCATTGCAAGGAGAGATTAAAAAGT
GAAGCCAAGAGGCTGAATGT

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEK
AEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQL
DIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
DGQGLDNVRSAMGQMESITHLDEVYQENHDSXANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRII
KREGNAEEPLVEGAPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEH
AKELLTQAHHFKQTAEAIQ

Sequences:

>Translated_579_residues
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEK
AEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQL
DIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
DGQGLDNVRSAMGQMESITHLDEVYQENHDSXANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRII
KREGNAEEPLVEGAPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEH
AKELLTQAHHFKQTAEAIQ
>Mature_579_residues
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEK
AEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQL
DIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
DGQGLDNVRSAMGQMESITHLDEVYQENHDSXANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNT
VEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRII
KREGNAEEPLVEGAPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEH
AKELLTQAHHFKQTAEAIQ

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=573, Percent_Identity=32.8097731239092, Blast_Score=294, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 65720; Mature: 65720

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTE
CCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLK
HHHCCEEEEEECCCCCHHHHHHHCCEEEECCEEEEEECCCCCCCCEEEECCEEEHHHHHH
EIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQLKSL
HHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DGQGLDNVRSAMGQMESITHLDEVYQENHDSXANSYYLLEEVAYQLREKLDMMEYDPNRL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
DEIETRLNEIRMLKRKYGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHH
KEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGAPVRLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEE
DGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
CCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDK
HHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHH
VTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTE
CCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFYVEDDKHPCIEKAEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLK
HHHCCEEEEEECCCCCHHHHHHHCCEEEECCEEEEEECCCCCCCCEEEECCEEEHHHHHH
EIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQLDIYQNVYADYEKLKKQLKSL
HHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DGQGLDNVRSAMGQMESITHLDEVYQENHDSXANSYYLLEEVAYQLREKLDMMEYDPNRL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
DEIETRLNEIRMLKRKYGNTVEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVIL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHH
KEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRIIKREGNAEEPLVEGAPVRLTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCEEEEE
DGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
CCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDK
HHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHH
VTEIARMISGVEITDLTTEHAKELLTQAHHFKQTAEAIQ
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]