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The map label for this gene is pbpB [H]

Identifier: 218905047

GI number: 218905047

Start: 3771441

End: 3773540

Strand: Reverse

Name: pbpB [H]

Synonym: BCAH820_3931

Alternate gene names: 218905047

Gene position: 3773540-3771441 (Counterclockwise)

Preceding gene: 218905048

Following gene: 218905046

Centisome position: 71.16

GC content: 37.81

Gene sequence:

>2100_bases
ATGATTTTATTCCTCCTGCTCTTTTTGCTGTTATTAGCCCGATTTTTTTACATACAAGCAACAGGATCAGTTCATAATCA
AGATTTGAATGAACTTGCTAAGCAAAAACATAGTAAAACAGGAGTTCTAGAAGCAAATCGCGGGACGATCTATGATCAAA
ATGGTCATGTATTAGCGCAAGATGCAAACTCTTATAAACTTGTAGCGGAATTAAAAGGTGCGAAGCCTGTTGAGAATAAA
GAGGATACTGCTAAGAAAATTGCGGGAGTACTCGGAACAGAAGAAGAGAAGATTTTAGCTACTTTAAATAAAGAGGGTAG
AAGTCAAGTAGAGTTTGGGAAATTAGGTAAAGGCTTGACGAAAGAACAGAAAGAACAAATAGAAGCATTAAAATTGCCAG
GTATATCTTTTATTACTGAAAATGCAAGAGTATATCCGAACGGTGATTTCGCATCTTATGTCGTAGGTCATGCGAGACCT
GATGATAAGGGGATTGCTATAGGGCAATTTGGTTTAGAAAAGAGTTTGGATAAGTATTTAGGAGCTTCTCATGGGGAAGT
AGCTTATACAGGTGATCGTAGAGGCGTATCTCTTGACGGTGGAAAAGTAAACGTAAAAGCACCTAAAAATGGAGATAATG
TATATTTAACGCTTGACCAACGTATTCAAAGTTATTTAGAAGATGCAATGAAAGAAGCGAGTAAACATTATGAACCAGAA
AGCTTAATAGGAATTGTTGCGGATCCAAAAACAGGGAAAATATTAGGAATGTCTAGTAAGCCTAGTTATGATCCGAACCA
GGAAAATAATCAGTATTTTTATAATGATGCAATCGCAAATGCATTTGAACCTGGATCAACAATGAAAATTTTCACACTAG
CAGCAGCTATTAATGAAGGTGTGTATAAGGGACAAGATTATTATCAGTCTGGTACATATCCAGTTGGGAACCGCAAAATA
AAAGATCATAATGGCGGCGCTGGATGGGGATCTATCACATTTGATGAAGGAGTAGAGCGCTCTTCAAACGTAGCATTTGC
AATTTTAGGGGATCAAAAACTTGGTCCAGAACGTTTTCGAAAATATATTCATAGCTTTGGATTAGATGAAAAAACAAAGA
TTGATTTACCTGGTGAAGGTTCAAATACCATTGTATTCGATCAGCAAATACAGCAAGTAACAACAGCTTTTGGACAAGGT
TCTACTGTAACACCAATTCAGCTTGTCCAAGCTGCAACTGCTATTGCGAACGATGGGAAAATGATGAAACCCTATACAAT
TGATAAAATTGTAGATCCGATAACAGAGGAAGTAAAATTAGAACATAAACCAGAAGAAGTGGGAAAACCTGTTACAAAAG
AGACAGCAGCGCAAGTAAGACAGTTATTAGAGCGTGTTGTTACATCACCGAAAGGAACAGGAACTGCTTATAAAGTCGAT
GGATATTCAGTTGGTGGTAAAACAGGAACAGCACAAATTCCGGATGGAAAAGGTGGCTATATGACGGGAAGAGAGAATTA
TATATTCTCGTTCTTAGGTATGGCACCTATGGACGATCCGCAACTTGTTGTGTATGTAGCTATTAAAAAGCCGAAATTGA
AAGATGATGAGAGCGGTGCACAACCTTTAGCTGATATTTTTAAATATGTAACGAAGAATAGTTTAGAGTATTTAAAGATT
AAGCCTAATGAAGTGAAAGATCCGAAGAAATATGTGAAAGAGCAACAAACTGCTGTTCCAGATGTAACCGAAAAAACGAT
GGAAGAAGCGAAAAAAGCCATTGATAAAGCAAAACTACGTCCAATCGTATTAGGTGAAGGGAAAGTACAGCAACAAGTGC
CGAAAGCAACTGAACAAACATTGAAGGGTGACCGAGTCTTCTTAGTGGGAGATAAACCTACAATGCCGAATATACAAGGC
TGGGCACTGCGTGATGTTATGAATTTAGCAAAAACATTAAAGCTTAACCTAAAACCTACTGGTACCGGATACGTTACTGA
ACAAAGTGTGCCAGAAGGAACATTGTTACAACCTGGTACAGAGTTAGGTGTAACACTTGTACCGCCACTTGAACCACAAC
AAGAAGCAGAAAAACCGTAA

Upstream 100 bases:

>100_bases
TGCAAATAATGTGAAAGGCCTCAAATAAGGCAATAGGTGTGAAGGGAAAATGAAGAGAAATATGACTAAATTTCATACCA
ATAAGGGAGCAGGGTATTTT

Downstream 100 bases:

>100_bases
TGGTAAAAGAGAAGATGTTCTAATTAAATAAGTACAAGCATATATTGGAACAAGCTAGGCGTAAGGGAGGCTTGTTCCAA
TATGCGTGTATCAAATGTAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MILFLLLFLLLLARFFYIQATGSVHNQDLNELAKQKHSKTGVLEANRGTIYDQNGHVLAQDANSYKLVAELKGAKPVENK
EDTAKKIAGVLGTEEEKILATLNKEGRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENARVYPNGDFASYVVGHARP
DDKGIAIGQFGLEKSLDKYLGASHGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQSYLEDAMKEASKHYEPE
SLIGIVADPKTGKILGMSSKPSYDPNQENNQYFYNDAIANAFEPGSTMKIFTLAAAINEGVYKGQDYYQSGTYPVGNRKI
KDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFRKYIHSFGLDEKTKIDLPGEGSNTIVFDQQIQQVTTAFGQG
STVTPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLLERVVTSPKGTGTAYKVD
GYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAIKKPKLKDDESGAQPLADIFKYVTKNSLEYLKI
KPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQG
WALRDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP

Sequences:

>Translated_699_residues
MILFLLLFLLLLARFFYIQATGSVHNQDLNELAKQKHSKTGVLEANRGTIYDQNGHVLAQDANSYKLVAELKGAKPVENK
EDTAKKIAGVLGTEEEKILATLNKEGRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENARVYPNGDFASYVVGHARP
DDKGIAIGQFGLEKSLDKYLGASHGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQSYLEDAMKEASKHYEPE
SLIGIVADPKTGKILGMSSKPSYDPNQENNQYFYNDAIANAFEPGSTMKIFTLAAAINEGVYKGQDYYQSGTYPVGNRKI
KDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFRKYIHSFGLDEKTKIDLPGEGSNTIVFDQQIQQVTTAFGQG
STVTPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLLERVVTSPKGTGTAYKVD
GYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAIKKPKLKDDESGAQPLADIFKYVTKNSLEYLKI
KPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQG
WALRDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP
>Mature_699_residues
MILFLLLFLLLLARFFYIQATGSVHNQDLNELAKQKHSKTGVLEANRGTIYDQNGHVLAQDANSYKLVAELKGAKPVENK
EDTAKKIAGVLGTEEEKILATLNKEGRSQVEFGKLGKGLTKEQKEQIEALKLPGISFITENARVYPNGDFASYVVGHARP
DDKGIAIGQFGLEKSLDKYLGASHGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQSYLEDAMKEASKHYEPE
SLIGIVADPKTGKILGMSSKPSYDPNQENNQYFYNDAIANAFEPGSTMKIFTLAAAINEGVYKGQDYYQSGTYPVGNRKI
KDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFRKYIHSFGLDEKTKIDLPGEGSNTIVFDQQIQQVTTAFGQG
STVTPIQLVQAATAIANDGKMMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLLERVVTSPKGTGTAYKVD
GYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAIKKPKLKDDESGAQPLADIFKYVTKNSLEYLKI
KPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQG
WALRDVMNLAKTLKLNLKPTGTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=588, Percent_Identity=27.5510204081633, Blast_Score=179, Evalue=7e-46,
Organism=Escherichia coli, GI1786854, Length=551, Percent_Identity=23.956442831216, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 76512; Mature: 76512

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILFLLLFLLLLARFFYIQATGSVHNQDLNELAKQKHSKTGVLEANRGTIYDQNGHVLAQ
CHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCEEEECCCEEEEE
DANSYKLVAELKGAKPVENKEDTAKKIAGVLGTEEEKILATLNKEGRSQVEFGKLGKGLT
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHCCCCC
KEQKEQIEALKLPGISFITENARVYPNGDFASYVVGHARPDDKGIAIGQFGLEKSLDKYL
HHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHEEECCCCCCCCEEEECCCHHHHHHHHH
GASHGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQSYLEDAMKEASKHYEPE
CCCCCCEEEECCCCCEEECCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCC
SLIGIVADPKTGKILGMSSKPSYDPNQENNQYFYNDAIANAFEPGSTMKIFTLAAAINEG
CEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEECHHHHHCCCCCCCEEHHHHHHHHHCC
VYKGQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFR
CCCCCHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCHHHHH
KYIHSFGLDEKTKIDLPGEGSNTIVFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGK
HHHHHCCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
MMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLLERVVTSPKGTGTAYKVD
EECCEEHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAIKKPKLKDDESGA
CEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCC
QPLADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLR
CHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
PIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQGWALRDVMNLAKTLKLNLKPT
EEEECCCHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHEEEECCCC
GTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP
CCCEEECCCCCCCCEECCCCCCCEEEECCCCCHHHCCCC
>Mature Secondary Structure
MILFLLLFLLLLARFFYIQATGSVHNQDLNELAKQKHSKTGVLEANRGTIYDQNGHVLAQ
CHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCCEECCCCEEEECCCEEEEE
DANSYKLVAELKGAKPVENKEDTAKKIAGVLGTEEEKILATLNKEGRSQVEFGKLGKGLT
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHCCCCC
KEQKEQIEALKLPGISFITENARVYPNGDFASYVVGHARPDDKGIAIGQFGLEKSLDKYL
HHHHHHHHHHCCCCCEEEECCCEEECCCCHHHHEEECCCCCCCCEEEECCCHHHHHHHHH
GASHGEVAYTGDRRGVSLDGGKVNVKAPKNGDNVYLTLDQRIQSYLEDAMKEASKHYEPE
CCCCCCEEEECCCCCEEECCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCC
SLIGIVADPKTGKILGMSSKPSYDPNQENNQYFYNDAIANAFEPGSTMKIFTLAAAINEG
CEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEECHHHHHCCCCCCCEEHHHHHHHHHCC
VYKGQDYYQSGTYPVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFR
CCCCCHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCHHHHH
KYIHSFGLDEKTKIDLPGEGSNTIVFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGK
HHHHHCCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
MMKPYTIDKIVDPITEEVKLEHKPEEVGKPVTKETAAQVRQLLERVVTSPKGTGTAYKVD
EECCEEHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GYSVGGKTGTAQIPDGKGGYMTGRENYIFSFLGMAPMDDPQLVVYVAIKKPKLKDDESGA
CEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCC
QPLADIFKYVTKNSLEYLKIKPNEVKDPKKYVKEQQTAVPDVTEKTMEEAKKAIDKAKLR
CHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
PIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQGWALRDVMNLAKTLKLNLKPT
EEEECCCHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHEEEECCCC
GTGYVTEQSVPEGTLLQPGTELGVTLVPPLEPQQEAEKP
CCCEEECCCCCCCCEECCCCCCCEEEECCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]