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The map label for this gene is pyrK

Identifier: 218905015

GI number: 218905015

Start: 3736509

End: 3737288

Strand: Reverse

Name: pyrK

Synonym: BCAH820_3899

Alternate gene names: 218905015

Gene position: 3737288-3736509 (Counterclockwise)

Preceding gene: 218905016

Following gene: 218905014

Centisome position: 70.48

GC content: 41.28

Gene sequence:

>780_bases
ATGATGCAAAAGCAAAATATGATCGTCGTTAATCAAAAAGAAATCGCAAAGAACATTTACGAATTAGTGCTTCAAGGAAC
GTTAGTACAGCAAATGAACGAACCAGGGCAGTTTGTACACATTAAGGTAGCAGAGGGCATTGCGCCCCTTCTGCGCCGCC
CAATTAGTATTTGTAATGTAGATCAAGAGAAGAACGAATTTACAATGCTATATCGTGCGGAAGGACAAGGGACAAAAACA
TTAGCAACTAGAAAACAAGGTGAAATGGTAGACGTACTAGGACCACTAGGACACGGTTTTCCTGTAGAAGAAGCAGAAGC
TGGTCAAACAGCTTTACTAGTAGGCGGGGGAATTGGTGTGCCACCACTTTATGAATTATCACAGCGCCTCGTTGCAAAAG
GTGTACGTGTGATTCACATCTTAGGTTTTCAAACGAAAGATGTAGTTTTCTATGAAGAAAAATTTGCAGAACTTGGTGAT
ACGTACGTTGCGACAGTAGACGGTACACACGGTACAAAAGGATTTGTCACAGATGTAATTGATCATTACGGAATTGACTT
TGATATTCTTTATTCATGTGGTCCGTTAGCGATGCTTCGTGCATTAGAAGGACGTTACAAAGAGAAAAAAGCCTATATTT
CATTAGAAGAACGTATGGGCTGTGGTATTGGAGCTTGTTTCGCATGTGTATGCCACTTACAAGAAGATCCAAGTGGACAT
TCTTACAAGAAGGTGTGTAGCGACGGACCAGTATTTCCAATCGGGGAGGTTGTACTATGA

Upstream 100 bases:

>100_bases
ATACAACGAGAGCAATCTTACGAGTATTAGAATCTATGACATTCTCAGCTCATTCAATGAAAGAAATTACGCAAACAAAG
CGTCACGAGGTGGTACATGC

Downstream 100 bases:

>100_bases
ACAGATTGCAAGTTGAATTACCAGGATTGTCATTAAAAAATCCAATTATACCGGCATCTGGATGCTTTGGATTTGGTCGT
GAATATGCACAGTTTTACGA

Product: dihydroorotate dehydrogenase electron transfer subunit

Products: orotate; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MMQKQNMIVVNQKEIAKNIYELVLQGTLVQQMNEPGQFVHIKVAEGIAPLLRRPISICNVDQEKNEFTMLYRAEGQGTKT
LATRKQGEMVDVLGPLGHGFPVEEAEAGQTALLVGGGIGVPPLYELSQRLVAKGVRVIHILGFQTKDVVFYEEKFAELGD
TYVATVDGTHGTKGFVTDVIDHYGIDFDILYSCGPLAMLRALEGRYKEKKAYISLEERMGCGIGACFACVCHLQEDPSGH
SYKKVCSDGPVFPIGEVVL

Sequences:

>Translated_259_residues
MMQKQNMIVVNQKEIAKNIYELVLQGTLVQQMNEPGQFVHIKVAEGIAPLLRRPISICNVDQEKNEFTMLYRAEGQGTKT
LATRKQGEMVDVLGPLGHGFPVEEAEAGQTALLVGGGIGVPPLYELSQRLVAKGVRVIHILGFQTKDVVFYEEKFAELGD
TYVATVDGTHGTKGFVTDVIDHYGIDFDILYSCGPLAMLRALEGRYKEKKAYISLEERMGCGIGACFACVCHLQEDPSGH
SYKKVCSDGPVFPIGEVVL
>Mature_259_residues
MMQKQNMIVVNQKEIAKNIYELVLQGTLVQQMNEPGQFVHIKVAEGIAPLLRRPISICNVDQEKNEFTMLYRAEGQGTKT
LATRKQGEMVDVLGPLGHGFPVEEAEAGQTALLVGGGIGVPPLYELSQRLVAKGVRVIHILGFQTKDVVFYEEKFAELGD
TYVATVDGTHGTKGFVTDVIDHYGIDFDILYSCGPLAMLRALEGRYKEKKAYISLEERMGCGIGACFACVCHLQEDPSGH
SYKKVCSDGPVFPIGEVVL

Specific function: Is responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the pyrD subunit to the ultimate electron acceptor NAD(+)

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRK_BACAA (C3P655)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868121.1
- ProteinModelPortal:   C3P655
- SMR:   C3P655
- EnsemblBacteria:   EBBACT00000126669
- GeneID:   7849449
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4047
- GeneTree:   EBGT00050000000799
- ProtClustDB:   PRK00054
- GO:   GO:0006810
- HAMAP:   MF_01211_B
- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938
- PIRSF:   PIRSF006816
- PRINTS:   PR00409

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1; SSF63380 Riboflavin_synthase_like_b-brl

EC number: 1.3.3.1

Molecular weight: Translated: 28439; Mature: 28439

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS51384 FAD_FR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQKQNMIVVNQKEIAKNIYELVLQGTLVQQMNEPGQFVHIKVAEGIAPLLRRPISICNV
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCHHHCCC
DQEKNEFTMLYRAEGQGTKTLATRKQGEMVDVLGPLGHGFPVEEAEAGQTALLVGGGIGV
CCCCCCEEEEEEECCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCC
PPLYELSQRLVAKGVRVIHILGFQTKDVVFYEEKFAELGDTYVATVDGTHGTKGFVTDVI
CCHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
DHYGIDFDILYSCGPLAMLRALEGRYKEKKAYISLEERMGCGIGACFACVCHLQEDPSGH
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEHHHCCCCHHHHHHHHHHHCCCCCCC
SYKKVCSDGPVFPIGEVVL
HHHHHHCCCCCCCCHHHCC
>Mature Secondary Structure
MMQKQNMIVVNQKEIAKNIYELVLQGTLVQQMNEPGQFVHIKVAEGIAPLLRRPISICNV
CCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCHHHCCC
DQEKNEFTMLYRAEGQGTKTLATRKQGEMVDVLGPLGHGFPVEEAEAGQTALLVGGGIGV
CCCCCCEEEEEEECCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCC
PPLYELSQRLVAKGVRVIHILGFQTKDVVFYEEKFAELGDTYVATVDGTHGTKGFVTDVI
CCHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
DHYGIDFDILYSCGPLAMLRALEGRYKEKKAYISLEERMGCGIGACFACVCHLQEDPSGH
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEHHHCCCCHHHHHHHHHHHCCCCCCC
SYKKVCSDGPVFPIGEVVL
HHHHHHCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-dihydroorotate; O2

Specific reaction: (S)-dihydroorotate + O2 = orotate + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA