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The map label for this gene is hsdS [H]

Identifier: 218901963

GI number: 218901963

Start: 806488

End: 807975

Strand: Direct

Name: hsdS [H]

Synonym: BCAH820_0845

Alternate gene names: 218901963

Gene position: 806488-807975 (Clockwise)

Preceding gene: 218901962

Following gene: 218901964

Centisome position: 15.21

GC content: 34.01

Gene sequence:

>1488_bases
ATGAGCAAGAAGAAAAAGACCCTCCAGGAATTATTAGAGGATGCATTAATTCCGACAGAAGAGCATCCGTATGAAGTCCC
AGGGAATTGGATATGGGGAAACTTAAATTCTTTATCAAAACTGATTGTGGACGGTTCACATAACCCACCTCCCAAAAAAA
ATGAAGGATTTCCAATGCTAAGTGGACGGAATATTCTTGATGGAGAAATTAACTTTGAAACTGATCGTTATGTAAGTGAG
GATGATTATCAAAAGGAGTATAAAAGAACACCTATTGAATCTAATGATGTGTTATTAACAATTGTCGGAACCATTGGGAG
AACGACTGTTGTACCTAAAGAATTTTCACCTTTTGTTTTACAAAGAAGTGTCGCACTAATTAAACCTATGGTGAATTCAA
ATTATTTATCATATTATTTTAGCTCTCCTTACTTTCAATATTATTTACAAAAGAATGCTAAAGGAACTGCACAGAAAGGA
GTTTATTTAAAAACCTTAAAGAGTAGCAGGATACCTTTACCACCTTTAATGGAACAAAAACGTATTACTGAGAAAGTAGA
GGGTTTGTTAGGAAGGGTTGAAGAAGCAAAAGCGTTGATTGAAGAAGCGAAAAAAACCTTTGAAGTTCGTCGGGCGACTA
TTTTAGATAAGGCGTTTCGTGGAGAGTTGTCTGCAAAGTGGCGTGAAGATAATAGAATTGCTGAGGATGCATCTTCACTT
TTAGAACGTATTCAAATCCAAAAAAGAAATAGTTCAATTAAATCGAATACATTAAAAATTACTTCAGTAATTAAGGAAGA
AGAACCATTTGAATTACCTAATGGGTGGACATGGGTTAGGTTAGGGGAAATTTCTTATTATGTAACAAGTGGTTCAAGGG
ACTGGTCAAAATATTATTCAGATGAAGGAGCCATGTTCATAAGAACGCAGGATATAAATAAGAACAGCTTAAATCTTAGT
GATGTTGCATATGTTTCATTACCAGAAAAAGTGGAGGGGAAAAGAAGCTTAGTCGAGAAGGCGGACATATTAACTACTAT
TACTGGAGCAAATGTTGGAAAATGTGCTTTAGTTGAGACAAATATTAAAGAGGCATATGTTAGTCAGTCTGTTGCACTTA
CTAAACTTATAGAAAAGTCTATTTCTAAATATGTTCATTTAAGTTTACTTTCGCCTTGTGGTGGAGGTAATGAGCTTGAA
GAAAGAGCGTATGGAATTGGTAGACCAGTTTTAAGCTTGGAGGATATTAAAAATATTAAAATTCCTTTGGCACCAATGGC
AGAACAACAAGTTATTGTTAAACTGGTTGAAACATTACTTGAAAATGAGAAGGAATCATTGAATCTAGCGAGTATAGAAA
AGCATTTAGAGACCTTAAAGCAATCCATATTGAATAAAGCGTTCCGCGGAGAATTAGGTACAAATGATCCAAATGAAGAA
AGTTCTATGAAATTGTTAAAAAAAGTATTACAAGAAAAAATTAAATAA

Upstream 100 bases:

>100_bases
CGGCAGAAGAAGCTATTGGAAAGTTACAGCAAGCTGTCACATTATTAAATGAAGTTGTGGATGAATTGAAAGCGGTGGAA
GAAGCTGAGGTGGTCAAGTA

Downstream 100 bases:

>100_bases
ATAAATTAACAAAAGGACATCTAACTTCAGGTGTCCTTTTGTTATACCTGTAACTTTACTCGCATAGAGTAATAGTAACT
TACAAATGTAACTTTATTCT

Product: restriction modification system DNA specificity domain protein

Products: NA

Alternate protein names: S.EcoBI; Type I restriction enzyme EcoBI specificity protein; S protein [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSKKKKTLQELLEDALIPTEEHPYEVPGNWIWGNLNSLSKLIVDGSHNPPPKKNEGFPMLSGRNILDGEINFETDRYVSE
DDYQKEYKRTPIESNDVLLTIVGTIGRTTVVPKEFSPFVLQRSVALIKPMVNSNYLSYYFSSPYFQYYLQKNAKGTAQKG
VYLKTLKSSRIPLPPLMEQKRITEKVEGLLGRVEEAKALIEEAKKTFEVRRATILDKAFRGELSAKWREDNRIAEDASSL
LERIQIQKRNSSIKSNTLKITSVIKEEEPFELPNGWTWVRLGEISYYVTSGSRDWSKYYSDEGAMFIRTQDINKNSLNLS
DVAYVSLPEKVEGKRSLVEKADILTTITGANVGKCALVETNIKEAYVSQSVALTKLIEKSISKYVHLSLLSPCGGGNELE
ERAYGIGRPVLSLEDIKNIKIPLAPMAEQQVIVKLVETLLENEKESLNLASIEKHLETLKQSILNKAFRGELGTNDPNEE
SSMKLLKKVLQEKIK

Sequences:

>Translated_495_residues
MSKKKKTLQELLEDALIPTEEHPYEVPGNWIWGNLNSLSKLIVDGSHNPPPKKNEGFPMLSGRNILDGEINFETDRYVSE
DDYQKEYKRTPIESNDVLLTIVGTIGRTTVVPKEFSPFVLQRSVALIKPMVNSNYLSYYFSSPYFQYYLQKNAKGTAQKG
VYLKTLKSSRIPLPPLMEQKRITEKVEGLLGRVEEAKALIEEAKKTFEVRRATILDKAFRGELSAKWREDNRIAEDASSL
LERIQIQKRNSSIKSNTLKITSVIKEEEPFELPNGWTWVRLGEISYYVTSGSRDWSKYYSDEGAMFIRTQDINKNSLNLS
DVAYVSLPEKVEGKRSLVEKADILTTITGANVGKCALVETNIKEAYVSQSVALTKLIEKSISKYVHLSLLSPCGGGNELE
ERAYGIGRPVLSLEDIKNIKIPLAPMAEQQVIVKLVETLLENEKESLNLASIEKHLETLKQSILNKAFRGELGTNDPNEE
SSMKLLKKVLQEKIK
>Mature_494_residues
SKKKKTLQELLEDALIPTEEHPYEVPGNWIWGNLNSLSKLIVDGSHNPPPKKNEGFPMLSGRNILDGEINFETDRYVSED
DYQKEYKRTPIESNDVLLTIVGTIGRTTVVPKEFSPFVLQRSVALIKPMVNSNYLSYYFSSPYFQYYLQKNAKGTAQKGV
YLKTLKSSRIPLPPLMEQKRITEKVEGLLGRVEEAKALIEEAKKTFEVRRATILDKAFRGELSAKWREDNRIAEDASSLL
ERIQIQKRNSSIKSNTLKITSVIKEEEPFELPNGWTWVRLGEISYYVTSGSRDWSKYYSDEGAMFIRTQDINKNSLNLSD
VAYVSLPEKVEGKRSLVEKADILTTITGANVGKCALVETNIKEAYVSQSVALTKLIEKSISKYVHLSLLSPCGGGNELEE
RAYGIGRPVLSLEDIKNIKIPLAPMAEQQVIVKLVETLLENEKESLNLASIEKHLETLKQSILNKAFRGELGTNDPNEES
SMKLLKKVLQEKIK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=496, Percent_Identity=22.5806451612903, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 56013; Mature: 55882

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKKKTLQELLEDALIPTEEHPYEVPGNWIWGNLNSLSKLIVDGSHNPPPKKNEGFPML
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCCCCCCCCCC
SGRNILDGEINFETDRYVSEDDYQKEYKRTPIESNDVLLTIVGTIGRTTVVPKEFSPFVL
CCCCEECCEECCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCEECCCCCCHHHH
QRSVALIKPMVNSNYLSYYFSSPYFQYYLQKNAKGTAQKGVYLKTLKSSRIPLPPLMEQK
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCHHCCEEEEECCCCCCCCCCCHHHH
RITEKVEGLLGRVEEAKALIEEAKKTFEVRRATILDKAFRGELSAKWREDNRIAEDASSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LERIQIQKRNSSIKSNTLKITSVIKEEEPFELPNGWTWVRLGEISYYVTSGSRDWSKYYS
HHHHHHHHHCCCHHCCCEEHHHHHCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHC
DEGAMFIRTQDINKNSLNLSDVAYVSLPEKVEGKRSLVEKADILTTITGANVGKCALVET
CCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEC
NIKEAYVSQSVALTKLIEKSISKYVHLSLLSPCGGGNELEERAYGIGRPVLSLEDIKNIK
CHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCCCCCHHHHHHHCCCCCCEEHHHHCCCC
IPLAPMAEQQVIVKLVETLLENEKESLNLASIEKHLETLKQSILNKAFRGELGTNDPNEE
CCCCCCHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SSMKLLKKVLQEKIK
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKKKKTLQELLEDALIPTEEHPYEVPGNWIWGNLNSLSKLIVDGSHNPPPKKNEGFPML
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCCCCCCCCCC
SGRNILDGEINFETDRYVSEDDYQKEYKRTPIESNDVLLTIVGTIGRTTVVPKEFSPFVL
CCCCEECCEECCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCEECCCCCCHHHH
QRSVALIKPMVNSNYLSYYFSSPYFQYYLQKNAKGTAQKGVYLKTLKSSRIPLPPLMEQK
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCHHCCEEEEECCCCCCCCCCCHHHH
RITEKVEGLLGRVEEAKALIEEAKKTFEVRRATILDKAFRGELSAKWREDNRIAEDASSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LERIQIQKRNSSIKSNTLKITSVIKEEEPFELPNGWTWVRLGEISYYVTSGSRDWSKYYS
HHHHHHHHHCCCHHCCCEEHHHHHCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHC
DEGAMFIRTQDINKNSLNLSDVAYVSLPEKVEGKRSLVEKADILTTITGANVGKCALVET
CCCEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEC
NIKEAYVSQSVALTKLIEKSISKYVHLSLLSPCGGGNELEERAYGIGRPVLSLEDIKNIK
CHHHHHHHHHHHHHHHHHHHHHHHEEHHHCCCCCCCCHHHHHHHCCCCCCEEHHHHCCCC
IPLAPMAEQQVIVKLVETLLENEKESLNLASIEKHLETLKQSILNKAFRGELGTNDPNEE
CCCCCCHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SSMKLLKKVLQEKIK
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321 [H]