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The map label for this gene is hsdM [H]

Identifier: 218901962

GI number: 218901962

Start: 805034

End: 806488

Strand: Direct

Name: hsdM [H]

Synonym: BCAH820_0844

Alternate gene names: 218901962

Gene position: 805034-806488 (Clockwise)

Preceding gene: 218901961

Following gene: 218901963

Centisome position: 15.18

GC content: 35.19

Gene sequence:

>1455_bases
ATGAACAATCAAGAAATTGTACAAAAACTATGGAATCTTTGTAACGTACTGCGTGATGATGGTATTACATATCAACAATA
CCTAACAGAATTAACATACATTTTATTTTTGAAGATGATGAATGAGAAGGGAAATTCCTCTGTTGAAGATAGAGTGAATA
TAGAGCATGTAATTCCAGAAGAGTATCGCTGGGAGAGCCTAGTCTCTCGTGAAGGAATAGAATTAAAAGAACATTATCAA
CGCCTTCTTTTAGAACTTGGAAGTTCAGATAATAGTATTTTACGACAAATTTATGCTGATGCAAGTACAAGCATTTCTGA
GCCGAAGAACTTGGAAAAAATTATTAAATCAATTGATAATTTAGATTGGTACAATGCAGAAAAAGAAGGACTTGGTGATT
TATACGAGGGCTTGCTCGAAAAAAACGCAAGTGAAACAAAGTCAGGGGCAGGTCAATACTTTACACCTCGTGTATTGATT
GATGTAATGGTGAAGCTTGTTGACCCTAAAATTGGAGAAAAATGCAGTGACCCAGCTGCAGGTACATTTGGGTTTATGAT
TGCTGCTGACCAATACTTGAAAAATCAAACTGATGATTACTTTGATGTTGATCCAGAACAAGCTGAGTTTCAAAAAACAG
AAGCATTTACAGGAATGGAACTTGTAAAAGACACACATCGTTTAGCTTTAATGAACGCTTTACTGCATGGCATCGAAGGA
CGTTTAGAGCAAGGAGATACATTATCAAGTAACGGGAAATGGATAAAGAATTTTGATGTGATTTTAACGAATCCTCCGTT
TGGTACTAAAAAGGGTGGAGAGCGTGCAACTCGTGATGACTTAACATTTGAAACATCGAATAAGCAGCTAAACTTCCTAC
AACTTATTTATAATGCATTAAAAGATGATGGAAAAGCCCGTGCAGCAGTTATTTTACCAGATAACGTGTTATTTGAAAGT
GGGATTGGTGCGCAAATTCGTCGTGATTTAATGGATAAATGTAATCTACATACAATTTTACGTTTGCCAACAGGAATTTT
CTATGCACAAGGTGTAAAAACGAATGTTTTATTCTTTACAAGAGAAAAAACAGATCGAAATAGTACGAAAGAAGTGTGGG
TATATGATCTGCGTACAAATATGCCATCATTTGGAAAGCGTAAACCTTTAACAGAAGGATACTTTGAAGAATTTATGACA
GCATACAATGCTGAAAATCGTTCAAAAGTAAAGAATGAGAGATGGAATGTCTTTACACGCGAAGAAATTGCAAAAAAAGA
GGATAGTTTAGATATTGGATTAATTGCTGATGATTCATTATCTGTTTATGAAAACTTACCAGATCCAATTAAATCGGCAG
AAGAAGCTATTGGAAAGTTACAGCAAGCTGTCACATTATTAAATGAAGTTGTGGATGAATTGAAAGCGGTGGAAGAAGCT
GAGGTGGTCAAGTAA

Upstream 100 bases:

>100_bases
ATTGACAAGATTGTGTATACTATAAATGATTACTTATATATTAGTTAACAATGTGCCTCTATCATGAGGCACACTTTTCA
TGTAAAGGAAGGGAAAATAA

Downstream 100 bases:

>100_bases
TGAGCAAGAAGAAAAAGACCCTCCAGGAATTATTAGAGGATGCATTAATTCCGACAGAAGAGCATCCGTATGAAGTCCCA
GGGAATTGGATATGGGGAAA

Product: type I restriction enzyme EcoKI M protein

Products: NA

Alternate protein names: M.EcoKI [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNNQEIVQKLWNLCNVLRDDGITYQQYLTELTYILFLKMMNEKGNSSVEDRVNIEHVIPEEYRWESLVSREGIELKEHYQ
RLLLELGSSDNSILRQIYADASTSISEPKNLEKIIKSIDNLDWYNAEKEGLGDLYEGLLEKNASETKSGAGQYFTPRVLI
DVMVKLVDPKIGEKCSDPAAGTFGFMIAADQYLKNQTDDYFDVDPEQAEFQKTEAFTGMELVKDTHRLALMNALLHGIEG
RLEQGDTLSSNGKWIKNFDVILTNPPFGTKKGGERATRDDLTFETSNKQLNFLQLIYNALKDDGKARAAVILPDNVLFES
GIGAQIRRDLMDKCNLHTILRLPTGIFYAQGVKTNVLFFTREKTDRNSTKEVWVYDLRTNMPSFGKRKPLTEGYFEEFMT
AYNAENRSKVKNERWNVFTREEIAKKEDSLDIGLIADDSLSVYENLPDPIKSAEEAIGKLQQAVTLLNEVVDELKAVEEA
EVVK

Sequences:

>Translated_484_residues
MNNQEIVQKLWNLCNVLRDDGITYQQYLTELTYILFLKMMNEKGNSSVEDRVNIEHVIPEEYRWESLVSREGIELKEHYQ
RLLLELGSSDNSILRQIYADASTSISEPKNLEKIIKSIDNLDWYNAEKEGLGDLYEGLLEKNASETKSGAGQYFTPRVLI
DVMVKLVDPKIGEKCSDPAAGTFGFMIAADQYLKNQTDDYFDVDPEQAEFQKTEAFTGMELVKDTHRLALMNALLHGIEG
RLEQGDTLSSNGKWIKNFDVILTNPPFGTKKGGERATRDDLTFETSNKQLNFLQLIYNALKDDGKARAAVILPDNVLFES
GIGAQIRRDLMDKCNLHTILRLPTGIFYAQGVKTNVLFFTREKTDRNSTKEVWVYDLRTNMPSFGKRKPLTEGYFEEFMT
AYNAENRSKVKNERWNVFTREEIAKKEDSLDIGLIADDSLSVYENLPDPIKSAEEAIGKLQQAVTLLNEVVDELKAVEEA
EVVK
>Mature_484_residues
MNNQEIVQKLWNLCNVLRDDGITYQQYLTELTYILFLKMMNEKGNSSVEDRVNIEHVIPEEYRWESLVSREGIELKEHYQ
RLLLELGSSDNSILRQIYADASTSISEPKNLEKIIKSIDNLDWYNAEKEGLGDLYEGLLEKNASETKSGAGQYFTPRVLI
DVMVKLVDPKIGEKCSDPAAGTFGFMIAADQYLKNQTDDYFDVDPEQAEFQKTEAFTGMELVKDTHRLALMNALLHGIEG
RLEQGDTLSSNGKWIKNFDVILTNPPFGTKKGGERATRDDLTFETSNKQLNFLQLIYNALKDDGKARAAVILPDNVLFES
GIGAQIRRDLMDKCNLHTILRLPTGIFYAQGVKTNVLFFTREKTDRNSTKEVWVYDLRTNMPSFGKRKPLTEGYFEEFMT
AYNAENRSKVKNERWNVFTREEIAKKEDSLDIGLIADDSLSVYENLPDPIKSAEEAIGKLQQAVTLLNEVVDELKAVEEA
EVVK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=529, Percent_Identity=46.8809073724008, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55155; Mature: 55155

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQEIVQKLWNLCNVLRDDGITYQQYLTELTYILFLKMMNEKGNSSVEDRVNIEHVIPE
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHCCCC
EYRWESLVSREGIELKEHYQRLLLELGSSDNSILRQIYADASTSISEPKNLEKIIKSIDN
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
LDWYNAEKEGLGDLYEGLLEKNASETKSGAGQYFTPRVLIDVMVKLVDPKIGEKCSDPAA
CCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCC
GTFGFMIAADQYLKNQTDDYFDVDPEQAEFQKTEAFTGMELVKDTHRLALMNALLHGIEG
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RLEQGDTLSSNGKWIKNFDVILTNPPFGTKKGGERATRDDLTFETSNKQLNFLQLIYNAL
CCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHH
KDDGKARAAVILPDNVLFESGIGAQIRRDLMDKCNLHTILRLPTGIFYAQGVKTNVLFFT
CCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHCCCHHHHHCCCCHHEECCCCEEEEEEE
REKTDRNSTKEVWVYDLRTNMPSFGKRKPLTEGYFEEFMTAYNAENRSKVKNERWNVFTR
ECCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCH
EEIAKKEDSLDIGLIADDSLSVYENLPDPIKSAEEAIGKLQQAVTLLNEVVDELKAVEEA
HHHHHCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVK
HCCC
>Mature Secondary Structure
MNNQEIVQKLWNLCNVLRDDGITYQQYLTELTYILFLKMMNEKGNSSVEDRVNIEHVIPE
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHCCCC
EYRWESLVSREGIELKEHYQRLLLELGSSDNSILRQIYADASTSISEPKNLEKIIKSIDN
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
LDWYNAEKEGLGDLYEGLLEKNASETKSGAGQYFTPRVLIDVMVKLVDPKIGEKCSDPAA
CCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCC
GTFGFMIAADQYLKNQTDDYFDVDPEQAEFQKTEAFTGMELVKDTHRLALMNALLHGIEG
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RLEQGDTLSSNGKWIKNFDVILTNPPFGTKKGGERATRDDLTFETSNKQLNFLQLIYNAL
CCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHH
KDDGKARAAVILPDNVLFESGIGAQIRRDLMDKCNLHTILRLPTGIFYAQGVKTNVLFFT
CCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHCCCHHHHHCCCCHHEECCCCEEEEEEE
REKTDRNSTKEVWVYDLRTNMPSFGKRKPLTEGYFEEFMTAYNAENRSKVKNERWNVFTR
ECCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCH
EEIAKKEDSLDIGLIADDSLSVYENLPDPIKSAEEAIGKLQQAVTLLNEVVDELKAVEEA
HHHHHCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3323532; 7610040; 9278503 [H]