Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 218884762

GI number: 218884762

Start: 1344563

End: 1345897

Strand: Direct

Name: gdhA [H]

Synonym: DKAM_1451

Alternate gene names: 218884762

Gene position: 1344563-1345897 (Clockwise)

Preceding gene: 218884756

Following gene: 218884764

Centisome position: 98.49

GC content: 45.47

Gene sequence:

>1335_bases
TTGATAGGTAGTCTTTTTAAAGCATCCAGGAATAATAGTTACTTGGTGATAAATATGAATGAGTATGCATCACTATATGA
AAACGATCCAGTATATCAGATGGCTGTGAAGCAATTACGTGAATCAGCATCGCTTCTAGGATTATCCGATGAAATCGTCG
AGATACTCAGACACCCGGAGAAACTGGTACAGGTTAAAATCACTATTAGGAGGGATAACGGTAAGCTAGAGACGTATCTA
GGCTGGAGAAGCCAGCACAATAGTGCGCTGGGACCTTATAAGGGAGGAGTAAGATACGGTGAAGACGTGACTCCTGGTGA
AGTAGTTGCTTTATCAATGTGGATGACCTGGAAGACCTCCCTAGCTGGCATTCCTTATGGCGGTGGAAAGGGGGGTGTAA
GGGTTAACCCGAAGGCTTTAAGCCAGAGAGAGCTAGAAGAGTTAAGCAGGAAGTTCTTTGCCGGCATAGCCAAGGACGTA
GGCCCCGAGGTAGATATACCTGCCCCAGACGTATACACTAATCCACAAACAATGGCATGGTACTTCGATGAATATAGTAA
GATAGTAGGGTACCAGGCGTGGGGAGTTGTTACCGCGAAGCCCGTGGAGCTTGGAGGATTATATGCAAGGGTTGTATCAA
CAGGGTATGGCACAGCTCTCACTGCAAGGGAGGCGGCTAAGAGATGGATAGGTGGATTGGCGGGTAAAACAGTTGCAATA
CATGGTTTCGGAAACGTTGGCATATATGCTGCGAAATATCTTACTGAATGGGGTGCAAGAGTGGTTGCTGTAAGTGATAG
CAGTGGATACATATATGATCCGAACGGATTAGACATCGAGGAAGCAATAAGGGTTAAGGAGACAACAGGCAAGGTGACAA
ACTATAAGAAGGGGGATGTAAAGGTCTCTGGAAACCATATGGAACTACTTGAATTACCCGTTGATATCCTAGTACCAGCT
GCAACACAGGATGTTATAACAAAGGAAAACGTTAATAGAATAAAGGCTAAAGTCATTGCTGAGGGCGCTAATGGTCCAAC
CACGCCTGAAGCCGAGAAGGTATTGTATGAGAAAGGTGTAATCATAGTGCCAGATATATTAGCGAACTCAGGAGGAGTAA
CGATGAGCTGGATCGAGTGGAGCCATAACAAGATGGGGTGTTGGCTCACTGATGAGGAGGCATTATCGAGGCTGGATAAA
ATGATGACGCTGAACTTTAATAGGGTGTACGATGAGTGGAGAAAGAGGTTCTCAGAATATCCAATGAGGACTGCTGCCTA
CGCTATAGCTGTAGACCGTGTCGTAAAGGCTATGAAGCTAAGGGGATGGATCTAA

Upstream 100 bases:

>100_bases
AAGCGATGAAGACCCGTGTGAGGGCTGATATTAACGGGATATGCATCACACGTTGTCCCATGCAACCTTGGAAGCGTATT
CCAGATATATATCGTATATA

Downstream 100 bases:

>100_bases
CCCTCGTTTTTTCCCCTCTTGTATAAAACTCTGTTATTTTTATCCCTGATCTCTTCAACCCTGTGCAATGGTATGATCTC
GTTATGTGTGTATATATATC

Product: Glu/Leu/Phe/Val dehydrogenase, C terminal

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MIGSLFKASRNNSYLVINMNEYASLYENDPVYQMAVKQLRESASLLGLSDEIVEILRHPEKLVQVKITIRRDNGKLETYL
GWRSQHNSALGPYKGGVRYGEDVTPGEVVALSMWMTWKTSLAGIPYGGGKGGVRVNPKALSQRELEELSRKFFAGIAKDV
GPEVDIPAPDVYTNPQTMAWYFDEYSKIVGYQAWGVVTAKPVELGGLYARVVSTGYGTALTAREAAKRWIGGLAGKTVAI
HGFGNVGIYAAKYLTEWGARVVAVSDSSGYIYDPNGLDIEEAIRVKETTGKVTNYKKGDVKVSGNHMELLELPVDILVPA
ATQDVITKENVNRIKAKVIAEGANGPTTPEAEKVLYEKGVIIVPDILANSGGVTMSWIEWSHNKMGCWLTDEEALSRLDK
MMTLNFNRVYDEWRKRFSEYPMRTAAYAIAVDRVVKAMKLRGWI

Sequences:

>Translated_444_residues
MIGSLFKASRNNSYLVINMNEYASLYENDPVYQMAVKQLRESASLLGLSDEIVEILRHPEKLVQVKITIRRDNGKLETYL
GWRSQHNSALGPYKGGVRYGEDVTPGEVVALSMWMTWKTSLAGIPYGGGKGGVRVNPKALSQRELEELSRKFFAGIAKDV
GPEVDIPAPDVYTNPQTMAWYFDEYSKIVGYQAWGVVTAKPVELGGLYARVVSTGYGTALTAREAAKRWIGGLAGKTVAI
HGFGNVGIYAAKYLTEWGARVVAVSDSSGYIYDPNGLDIEEAIRVKETTGKVTNYKKGDVKVSGNHMELLELPVDILVPA
ATQDVITKENVNRIKAKVIAEGANGPTTPEAEKVLYEKGVIIVPDILANSGGVTMSWIEWSHNKMGCWLTDEEALSRLDK
MMTLNFNRVYDEWRKRFSEYPMRTAAYAIAVDRVVKAMKLRGWI
>Mature_444_residues
MIGSLFKASRNNSYLVINMNEYASLYENDPVYQMAVKQLRESASLLGLSDEIVEILRHPEKLVQVKITIRRDNGKLETYL
GWRSQHNSALGPYKGGVRYGEDVTPGEVVALSMWMTWKTSLAGIPYGGGKGGVRVNPKALSQRELEELSRKFFAGIAKDV
GPEVDIPAPDVYTNPQTMAWYFDEYSKIVGYQAWGVVTAKPVELGGLYARVVSTGYGTALTAREAAKRWIGGLAGKTVAI
HGFGNVGIYAAKYLTEWGARVVAVSDSSGYIYDPNGLDIEEAIRVKETTGKVTNYKKGDVKVSGNHMELLELPVDILVPA
ATQDVITKENVNRIKAKVIAEGANGPTTPEAEKVLYEKGVIIVPDILANSGGVTMSWIEWSHNKMGCWLTDEEALSRLDK
MMTLNFNRVYDEWRKRFSEYPMRTAAYAIAVDRVVKAMKLRGWI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=345, Percent_Identity=40.2898550724638, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI4885281, Length=345, Percent_Identity=39.7101449275362, Blast_Score=272, Evalue=4e-73,
Organism=Escherichia coli, GI1788059, Length=360, Percent_Identity=33.3333333333333, Blast_Score=188, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17544676, Length=337, Percent_Identity=40.3560830860534, Blast_Score=260, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324951, Length=430, Percent_Identity=30.2325581395349, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6319256, Length=384, Percent_Identity=31.7708333333333, Blast_Score=145, Evalue=9e-36,
Organism=Drosophila melanogaster, GI45549226, Length=345, Percent_Identity=39.7101449275362, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI45553475, Length=345, Percent_Identity=39.7101449275362, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24649283, Length=345, Percent_Identity=40.5797101449275, Blast_Score=254, Evalue=7e-68,
Organism=Drosophila melanogaster, GI24649460, Length=282, Percent_Identity=40.0709219858156, Blast_Score=218, Evalue=5e-57,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 49207; Mature: 49207

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGSLFKASRNNSYLVINMNEYASLYENDPVYQMAVKQLRESASLLGLSDEIVEILRHPE
CCCCCHHCCCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
KLVQVKITIRRDNGKLETYLGWRSQHNSALGPYKGGVRYGEDVTPGEVVALSMWMTWKTS
EEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHH
LAGIPYGGGKGGVRVNPKALSQRELEELSRKFFAGIAKDVGPEVDIPAPDVYTNPQTMAW
CCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
YFDEYSKIVGYQAWGVVTAKPVELGGLYARVVSTGYGTALTAREAAKRWIGGLAGKTVAI
EHHHHHHHHCEEECCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEE
HGFGNVGIYAAKYLTEWGARVVAVSDSSGYIYDPNGLDIEEAIRVKETTGKVTNYKKGDV
EECCCCHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCE
KVSGNHMELLELPVDILVPAATQDVITKENVNRIKAKVIAEGANGPTTPEAEKVLYEKGV
EECCCCEEEECCCHHHEECCCCHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHCCC
IIVPDILANSGGVTMSWIEWSHNKMGCWLTDEEALSRLDKMMTLNFNRVYDEWRKRFSEY
EEECHHHCCCCCCEEEEEEECCCCEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
PMRTAAYAIAVDRVVKAMKLRGWI
CHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIGSLFKASRNNSYLVINMNEYASLYENDPVYQMAVKQLRESASLLGLSDEIVEILRHPE
CCCCCHHCCCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
KLVQVKITIRRDNGKLETYLGWRSQHNSALGPYKGGVRYGEDVTPGEVVALSMWMTWKTS
EEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHH
LAGIPYGGGKGGVRVNPKALSQRELEELSRKFFAGIAKDVGPEVDIPAPDVYTNPQTMAW
CCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
YFDEYSKIVGYQAWGVVTAKPVELGGLYARVVSTGYGTALTAREAAKRWIGGLAGKTVAI
EHHHHHHHHCEEECCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEE
HGFGNVGIYAAKYLTEWGARVVAVSDSSGYIYDPNGLDIEEAIRVKETTGKVTNYKKGDV
EECCCCHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCE
KVSGNHMELLELPVDILVPAATQDVITKENVNRIKAKVIAEGANGPTTPEAEKVLYEKGV
EECCCCEEEECCCHHHEECCCCHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHCCC
IIVPDILANSGGVTMSWIEWSHNKMGCWLTDEEALSRLDKMMTLNFNRVYDEWRKRFSEY
EEECHHHCCCCCCEEEEEEECCCCEEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
PMRTAAYAIAVDRVVKAMKLRGWI
CHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]