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The map label for this gene is dphB [H]

Identifier: 218884756

GI number: 218884756

Start: 1339317

End: 1340090

Strand: Direct

Name: dphB [H]

Synonym: DKAM_1445

Alternate gene names: 218884756

Gene position: 1339317-1340090 (Clockwise)

Preceding gene: 218884754

Following gene: 218884762

Centisome position: 98.1

GC content: 42.76

Gene sequence:

>774_bases
GTGTCCCCCTTGCTTATCTTCATTGGTGCTGGTTATTCACGCAGACATTTAACTCAGGAAGCATTGGAGTCTTTGAAGAG
TGCTGAAAAGATATATGTTGACACCTATACATCAATGTATGAGGACGGCTTCAACTGGCTTAGAGATGTTAATCCATCAG
CTGAGATAGTGTTTGCTAAAAGAAGGGACTTAGAAGGGGAGGGTATTAATAGAATAGTAGAAGAGGCTAAGGAGGGTAAT
ATCGCGATACTCTGTGCCGGAGATCCATTTACAGCAACAACACATGACGCTATTAGGGTTGAAGCATTAAAGAGCAATGT
AAGCGTTAAGGTTGTAACCGGTATATCGGTGGTGAACCTAGTTCACAGCAGGATCGGCCTCCAAGCATATAGGTTCGGTA
AAATAGTCACCCTAGTATATCCTGACAGCTTTAAACCATACAGTGTTATCAAAACCATATATGATAATCTAGGTAGAAAC
CTCCATACACTCGTACTGCTCGATCTCAGGTTAGAGGAGGGGTTAGCGATGAGTATTCCAGAGGCTATAGATATACTTAC
GGAGCTCGACGAGAAACAACAATTAATGAATCAAATAAGCATTGGCGTGGCTAGGCTTGGATGGAGTAGTGAGGTAATTA
GGGCTGGGAGGCTTATCGAGTTAAAAAGATATAGTTATCCTCCTCCTCCTCATTCACTAATCATCGCTGCCAGGCTTCAT
CCAATAGAGTTAGAGAGTCTCAGGTATATAGCTGGCCTCACGATAACGGGTTAA

Upstream 100 bases:

>100_bases
AAAGCATTCTGAAAAAGCTACAAACCAGCATGAAAATAAAACAATAGTAGAAGGCATTTTTATTTAGATCTTCCATATGT
AGTTTTACAGTGTAGGGCTG

Downstream 100 bases:

>100_bases
CTCCGCGTGACGAAGAAAATAAATAGTGTCTAAGGTGAGGGGTTCGGGTCTGGTGAATACCTGTAATTGCCTGCTTCACC
CCTTATTTCTTATTCCCCTG

Product: putative diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSPLLIFIGAGYSRRHLTQEALESLKSAEKIYVDTYTSMYEDGFNWLRDVNPSAEIVFAKRRDLEGEGINRIVEEAKEGN
IAILCAGDPFTATTHDAIRVEALKSNVSVKVVTGISVVNLVHSRIGLQAYRFGKIVTLVYPDSFKPYSVIKTIYDNLGRN
LHTLVLLDLRLEEGLAMSIPEAIDILTELDEKQQLMNQISIGVARLGWSSEVIRAGRLIELKRYSYPPPPHSLIIAARLH
PIELESLRYIAGLTITG

Sequences:

>Translated_257_residues
MSPLLIFIGAGYSRRHLTQEALESLKSAEKIYVDTYTSMYEDGFNWLRDVNPSAEIVFAKRRDLEGEGINRIVEEAKEGN
IAILCAGDPFTATTHDAIRVEALKSNVSVKVVTGISVVNLVHSRIGLQAYRFGKIVTLVYPDSFKPYSVIKTIYDNLGRN
LHTLVLLDLRLEEGLAMSIPEAIDILTELDEKQQLMNQISIGVARLGWSSEVIRAGRLIELKRYSYPPPPHSLIIAARLH
PIELESLRYIAGLTITG
>Mature_256_residues
SPLLIFIGAGYSRRHLTQEALESLKSAEKIYVDTYTSMYEDGFNWLRDVNPSAEIVFAKRRDLEGEGINRIVEEAKEGNI
AILCAGDPFTATTHDAIRVEALKSNVSVKVVTGISVVNLVHSRIGLQAYRFGKIVTLVYPDSFKPYSVIKTIYDNLGRNL
HTLVLLDLRLEEGLAMSIPEAIDILTELDEKQQLMNQISIGVARLGWSSEVIRAGRLIELKRYSYPPPPHSLIIAARLHP
IELESLRYIAGLTITG

Specific function: Required for the methylation step in diphthamide biosynthesis [H]

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family [H]

Homologues:

Organism=Homo sapiens, GI117190324, Length=269, Percent_Identity=30.1115241635688, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI117190326, Length=270, Percent_Identity=30, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI117190262, Length=270, Percent_Identity=30, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531369, Length=268, Percent_Identity=29.4776119402985, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323201, Length=256, Percent_Identity=32.03125, Blast_Score=118, Evalue=8e-28,
Organism=Drosophila melanogaster, GI28572120, Length=272, Percent_Identity=32.3529411764706, Blast_Score=121, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.98 [H]

Molecular weight: Translated: 28693; Mature: 28561

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLLIFIGAGYSRRHLTQEALESLKSAEKIYVDTYTSMYEDGFNWLRDVNPSAEIVFAK
CCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEE
RRDLEGEGINRIVEEAKEGNIAILCAGDPFTATTHDAIRVEALKSNVSVKVVTGISVVNL
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHEEEEEHHCCCEEEEEECHHHHHH
VHSRIGLQAYRFGKIVTLVYPDSFKPYSVIKTIYDNLGRNLHTLVLLDLRLEEGLAMSIP
HHHHHCHHEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEECCH
EAIDILTELDEKQQLMNQISIGVARLGWSSEVIRAGRLIELKRYSYPPPPHSLIIAARLH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEECCCCCCCCCCEEEEEECC
PIELESLRYIAGLTITG
CCCHHHHHEEEEEEECC
>Mature Secondary Structure 
SPLLIFIGAGYSRRHLTQEALESLKSAEKIYVDTYTSMYEDGFNWLRDVNPSAEIVFAK
CCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEE
RRDLEGEGINRIVEEAKEGNIAILCAGDPFTATTHDAIRVEALKSNVSVKVVTGISVVNL
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHEEEEEHHCCCEEEEEECHHHHHH
VHSRIGLQAYRFGKIVTLVYPDSFKPYSVIKTIYDNLGRNLHTLVLLDLRLEEGLAMSIP
HHHHHCHHEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEECCH
EAIDILTELDEKQQLMNQISIGVARLGWSSEVIRAGRLIELKRYSYPPPPHSLIIAARLH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEECCCCCCCCCCEEEEEECC
PIELESLRYIAGLTITG
CCCHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA