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The map label for this gene is priA [H]

Identifier: 218884731

GI number: 218884731

Start: 1317672

End: 1318796

Strand: Direct

Name: priA [H]

Synonym: DKAM_1420

Alternate gene names: 218884731

Gene position: 1317672-1318796 (Clockwise)

Preceding gene: 218884730

Following gene: 218884732

Centisome position: 96.52

GC content: 44.62

Gene sequence:

>1125_bases
TTGCCCCACGGGAAGACTAGCGACTTCTTGAAGAAGGTCATAAGGGACTACTATAGGTTCAGACCTCTCGAAGAACCAGG
CAACCTACATATGAGAGAGATTGCTTTAGAGAGCCTTGAGGATCGCAGGTATATCAGGCACCTATCCTTCCCATATATGG
AGCAATTATATAACTTCATAGAGACCATGAAAACACCTCTCCACCTGTATTATTCTTCAGCCCTCTACAGCAATCCCTCG
GCCGAGGAAATGGAGGCCAAAGGGTGGCAGGGCTCGGAGTTAATATTCGATATAGATGCTGACAAGTATCCTGGATGCAT
ACAGAGGTATTTAGTCTGCAGTAAGTCAGGAGTAATAATTAATGATGGGGAGTCCTGTCCCTCAGGCGAGGAACCTTTGA
AATATTCCCCAGTGAGCTGGGAGTGCATCTTGAAAGCCTGGGACGACGCCTTAAAACTACGTGATATACTGGTGCAGGAC
TTCGGGTTTAGCAAGGTTAAAATATATTTCTCGGGTAACAGGGGATTCCATGTAAGAGTGATGGATGAGGAGGCACTGCC
TCTAACACGTGAGCAGAGGCGATTAATAGCGGATTATGTGGCATGTAGCAACATAGATGTGGACAGGATCTTCCCATCAT
ACAAAGATATAGTGGTTTTCCGCAGAGTTGAATATGGCCTGAGAAGAAGGGTGCTCGAGTTAGCTATATCGAGGAAGCTA
GTGAAGGAAAAAAGCGTAGCGGGTTTAAGGGGATTAATAATCAATAAGAGCGCCCTCGATGAATTAATTCGGGAGAACTG
TGTTAAAGTAGATAAGGTTGTAACAATGGATACGTCAAGGCTCTCAAGATTCGTTAACAGCTTAAACATGAAGTCCGGGT
TAAGGGTTGTAGAGGTTGAGCCAACCGTGGATCTAAGAGGAAAGTGTTTCACTGATTTCTCACCATTCCATGGGGAAGTC
ATGGTTAAACCCTTGGTCGATGCTTCTCAGATCCTGGTTCTAGATAAATTTATTGGGCTTAGAAAGGGAGAAATAATTAG
ATTAGAGGCACCCTATGCCCTATATCTAGCTGTCAATGGGCTAGTAGAGGTAAGGGATTCATCGGGGATTGAGGTAAAAC
TATGA

Upstream 100 bases:

>100_bases
GCTTCTCAGGCTGCTGAATTAATGGAGATAGAATTCGGTAATGGAACACCGCTCAAGCTTACATACGAGATACCCGGCGG
AGGAAGACTAGTGTTTTATG

Downstream 100 bases:

>100_bases
GTAACACATATATCCGCTTAATTAAAGAGGAATTACAGGCATCAAAGTGTACCCCTGTCCAGAAGATTAGACTAGATGAA
GTAAACGAGTTAATCAGGAG

Product: putative DNA primase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MPHGKTSDFLKKVIRDYYRFRPLEEPGNLHMREIALESLEDRRYIRHLSFPYMEQLYNFIETMKTPLHLYYSSALYSNPS
AEEMEAKGWQGSELIFDIDADKYPGCIQRYLVCSKSGVIINDGESCPSGEEPLKYSPVSWECILKAWDDALKLRDILVQD
FGFSKVKIYFSGNRGFHVRVMDEEALPLTREQRRLIADYVACSNIDVDRIFPSYKDIVVFRRVEYGLRRRVLELAISRKL
VKEKSVAGLRGLIINKSALDELIRENCVKVDKVVTMDTSRLSRFVNSLNMKSGLRVVEVEPTVDLRGKCFTDFSPFHGEV
MVKPLVDASQILVLDKFIGLRKGEIIRLEAPYALYLAVNGLVEVRDSSGIEVKL

Sequences:

>Translated_374_residues
MPHGKTSDFLKKVIRDYYRFRPLEEPGNLHMREIALESLEDRRYIRHLSFPYMEQLYNFIETMKTPLHLYYSSALYSNPS
AEEMEAKGWQGSELIFDIDADKYPGCIQRYLVCSKSGVIINDGESCPSGEEPLKYSPVSWECILKAWDDALKLRDILVQD
FGFSKVKIYFSGNRGFHVRVMDEEALPLTREQRRLIADYVACSNIDVDRIFPSYKDIVVFRRVEYGLRRRVLELAISRKL
VKEKSVAGLRGLIINKSALDELIRENCVKVDKVVTMDTSRLSRFVNSLNMKSGLRVVEVEPTVDLRGKCFTDFSPFHGEV
MVKPLVDASQILVLDKFIGLRKGEIIRLEAPYALYLAVNGLVEVRDSSGIEVKL
>Mature_373_residues
PHGKTSDFLKKVIRDYYRFRPLEEPGNLHMREIALESLEDRRYIRHLSFPYMEQLYNFIETMKTPLHLYYSSALYSNPSA
EEMEAKGWQGSELIFDIDADKYPGCIQRYLVCSKSGVIINDGESCPSGEEPLKYSPVSWECILKAWDDALKLRDILVQDF
GFSKVKIYFSGNRGFHVRVMDEEALPLTREQRRLIADYVACSNIDVDRIFPSYKDIVVFRRVEYGLRRRVLELAISRKLV
KEKSVAGLRGLIINKSALDELIRENCVKVDKVVTMDTSRLSRFVNSLNMKSGLRVVEVEPTVDLRGKCFTDFSPFHGEVM
VKPLVDASQILVLDKFIGLRKGEIIRLEAPYALYLAVNGLVEVRDSSGIEVKL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG1467

COG function: function code L; Eukaryotic-type DNA primase, catalytic (small) subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase small subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002755 [H]

Pfam domain/function: PF01896 DNA_primase_S [H]

EC number: 2.7.7.-

Molecular weight: Translated: 43015; Mature: 42884

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHGKTSDFLKKVIRDYYRFRPLEEPGNLHMREIALESLEDRRYIRHLSFPYMEQLYNFI
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ETMKTPLHLYYSSALYSNPSAEEMEAKGWQGSELIFDIDADKYPGCIQRYLVCSKSGVII
HHHCCCHHEEEHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEE
NDGESCPSGEEPLKYSPVSWECILKAWDDALKLRDILVQDFGFSKVKIYFSGNRGFHVRV
ECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEE
MDEEALPLTREQRRLIADYVACSNIDVDRIFPSYKDIVVFRRVEYGLRRRVLELAISRKL
ECCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKEKSVAGLRGLIINKSALDELIRENCVKVDKVVTMDTSRLSRFVNSLNMKSGLRVVEVE
HHHHHHCCHHHEEECHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHCCHHCCCEEEEEC
PTVDLRGKCFTDFSPFHGEVMVKPLVDASQILVLDKFIGLRKGEIIRLEAPYALYLAVNG
CCCCCCCCEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCEEEEEEECC
LVEVRDSSGIEVKL
EEEEECCCCCEEEC
>Mature Secondary Structure 
PHGKTSDFLKKVIRDYYRFRPLEEPGNLHMREIALESLEDRRYIRHLSFPYMEQLYNFI
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ETMKTPLHLYYSSALYSNPSAEEMEAKGWQGSELIFDIDADKYPGCIQRYLVCSKSGVII
HHHCCCHHEEEHHHHCCCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEE
NDGESCPSGEEPLKYSPVSWECILKAWDDALKLRDILVQDFGFSKVKIYFSGNRGFHVRV
ECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEE
MDEEALPLTREQRRLIADYVACSNIDVDRIFPSYKDIVVFRRVEYGLRRRVLELAISRKL
ECCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKEKSVAGLRGLIINKSALDELIRENCVKVDKVVTMDTSRLSRFVNSLNMKSGLRVVEVE
HHHHHHCCHHHEEECHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHCCHHCCCEEEEEC
PTVDLRGKCFTDFSPFHGEVMVKPLVDASQILVLDKFIGLRKGEIIRLEAPYALYLAVNG
CCCCCCCCEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCEEEEEEECC
LVEVRDSSGIEVKL
EEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA