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The map label for this gene is 218884723

Identifier: 218884723

GI number: 218884723

Start: 1312863

End: 1313888

Strand: Direct

Name: 218884723

Synonym: DKAM_1412

Alternate gene names: NA

Gene position: 1312863-1313888 (Clockwise)

Preceding gene: 218884722

Following gene: 218884724

Centisome position: 96.16

GC content: 48.05

Gene sequence:

>1026_bases
ATGGGATTAAAAGGCGTGTTATTCATGGAGGACCTATGTAGCCTCTATGATATCGAGCTCGATGTATTATATAGGCTTGC
TGAGAAAGGCGTGAAAAGGGTTCTTCTTCACGCGCCAGACGGCTTTAAGCCTTTATATAAATGCGTCGCCAGTGTTTTCG
AAGGAAGAGGTATTGAAATATTCTTTTCCTCGAGCCCTGGATATGGAGCATGCGATGTCCCTGTGGAGGAGGCTGAGGCC
CTCGGGGTCGACGTCCTTATTCACCTGGGGCATCTAGAGTATTTTAACCATGGGTTTAAGCCTGGTGTAGGCATTGTTTA
CGTACCCGTCTATGCCCGTCCAATGATTAGTGAGGGGGTGCTTGGAGAGCTGACTAGTTACCTCAGATCACGGGGGATTA
TAGGTGTTTCCATCTCCTCAACAATTGTAGAGGAGAAAATAAGAAGGATTATTGTGGAACACCTCAGCTCAAATGGGTTT
AGGATTCATGAGGTACACAGCCCTCTACTGGGTTGCCTCTATACACATGTAGCATCACTGGATGACAAAGTCGATGCTCA
CCTCGTGATAGCCGGTGGGATATTCCATCCCCTAGGTCTTGCACTGGTTTCCAGGAAGCCTGTAGTAGTCTTAGATCCCT
ACAGGGGTAGGGTGTGGTCAGCTAGTGAGGAGGCTCAGAAGACCCTTAGGAAGAGGCTGCTGAAGATCTACGAGGCTAAG
ATAAGCGGTGTTAGGGTTGGACTTATCATTGGGTCTAGGCTGGGCCAGTATCGTCCCTGGCTTATTGAGAAACTCGAGAG
GGAAGCCTTGGAGAAAGGGTATAGGGTCTACAAGGTTTCCTCAACGTATCTTAGTATTGAGCGATTAATAGCCATAGATA
ACGCCCTCAATCTCGACATATATGTTGTCACGAGTTGCCCGAGGCTCCCCATAGACGACTTAGATGACTTCTATAAGCCG
GTGTTAACCCCTGGCGAATTCCTGATGCTTCTAACAGGCTCGGAGAAATATGTATACCCGTGGTGA

Upstream 100 bases:

>100_bases
GCGTGCTCTCTTCTACAGGACCTGGGAACTAGGTTATCCTTATATTTGGAAACTCTTGTTTTTCCAGGTAGAATAATATT
AAGTCTTCCACATTGGTATA

Downstream 100 bases:

>100_bases
GCAATGGGGCTTATTAGGAGTAAGAAGGAGCTAGAGTTGATTCTCTCACGGATCAACTGGTACATGCACTCGAAGAAGAG
GCTTGAGCAATACCAGACAC

Product: diphthamide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MGLKGVLFMEDLCSLYDIELDVLYRLAEKGVKRVLLHAPDGFKPLYKCVASVFEGRGIEIFFSSSPGYGACDVPVEEAEA
LGVDVLIHLGHLEYFNHGFKPGVGIVYVPVYARPMISEGVLGELTSYLRSRGIIGVSISSTIVEEKIRRIIVEHLSSNGF
RIHEVHSPLLGCLYTHVASLDDKVDAHLVIAGGIFHPLGLALVSRKPVVVLDPYRGRVWSASEEAQKTLRKRLLKIYEAK
ISGVRVGLIIGSRLGQYRPWLIEKLEREALEKGYRVYKVSSTYLSIERLIAIDNALNLDIYVVTSCPRLPIDDLDDFYKP
VLTPGEFLMLLTGSEKYVYPW

Sequences:

>Translated_341_residues
MGLKGVLFMEDLCSLYDIELDVLYRLAEKGVKRVLLHAPDGFKPLYKCVASVFEGRGIEIFFSSSPGYGACDVPVEEAEA
LGVDVLIHLGHLEYFNHGFKPGVGIVYVPVYARPMISEGVLGELTSYLRSRGIIGVSISSTIVEEKIRRIIVEHLSSNGF
RIHEVHSPLLGCLYTHVASLDDKVDAHLVIAGGIFHPLGLALVSRKPVVVLDPYRGRVWSASEEAQKTLRKRLLKIYEAK
ISGVRVGLIIGSRLGQYRPWLIEKLEREALEKGYRVYKVSSTYLSIERLIAIDNALNLDIYVVTSCPRLPIDDLDDFYKP
VLTPGEFLMLLTGSEKYVYPW
>Mature_340_residues
GLKGVLFMEDLCSLYDIELDVLYRLAEKGVKRVLLHAPDGFKPLYKCVASVFEGRGIEIFFSSSPGYGACDVPVEEAEAL
GVDVLIHLGHLEYFNHGFKPGVGIVYVPVYARPMISEGVLGELTSYLRSRGIIGVSISSTIVEEKIRRIIVEHLSSNGFR
IHEVHSPLLGCLYTHVASLDDKVDAHLVIAGGIFHPLGLALVSRKPVVVLDPYRGRVWSASEEAQKTLRKRLLKIYEAKI
SGVRVGLIIGSRLGQYRPWLIEKLEREALEKGYRVYKVSSTYLSIERLIAIDNALNLDIYVVTSCPRLPIDDLDDFYKPV
LTPGEFLMLLTGSEKYVYPW

Specific function: Unknown

COG id: COG1736

COG function: function code J; Diphthamide synthase subunit DPH2

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016435
- InterPro:   IPR002728
- InterPro:   IPR022428 [H]

Pfam domain/function: PF01866 Diphthamide_syn [H]

EC number: NA

Molecular weight: Translated: 38138; Mature: 38007

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKGVLFMEDLCSLYDIELDVLYRLAEKGVKRVLLHAPDGFKPLYKCVASVFEGRGIEI
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCEEE
FFSSSPGYGACDVPVEEAEALGVDVLIHLGHLEYFNHGFKPGVGIVYVPVYARPMISEGV
EEECCCCCCCCCCCHHHHHHCCCEEEEEEHHHHHHCCCCCCCCCEEEECCCCCCHHCCCH
LGELTSYLRSRGIIGVSISSTIVEEKIRRIIVEHLSSNGFRIHEVHSPLLGCLYTHVASL
HHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCC
DDKVDAHLVIAGGIFHPLGLALVSRKPVVVLDPYRGRVWSASEEAQKTLRKRLLKIYEAK
CCCCCEEEEEECCHHHHHHHHHHCCCCEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHH
ISGVRVGLIIGSRLGQYRPWLIEKLEREALEKGYRVYKVSSTYLSIERLIAIDNALNLDI
HCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCCEEE
YVVTSCPRLPIDDLDDFYKPVLTPGEFLMLLTGSEKYVYPW
EEEECCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCEECCC
>Mature Secondary Structure 
GLKGVLFMEDLCSLYDIELDVLYRLAEKGVKRVLLHAPDGFKPLYKCVASVFEGRGIEI
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCEEE
FFSSSPGYGACDVPVEEAEALGVDVLIHLGHLEYFNHGFKPGVGIVYVPVYARPMISEGV
EEECCCCCCCCCCCHHHHHHCCCEEEEEEHHHHHHCCCCCCCCCEEEECCCCCCHHCCCH
LGELTSYLRSRGIIGVSISSTIVEEKIRRIIVEHLSSNGFRIHEVHSPLLGCLYTHVASL
HHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCC
DDKVDAHLVIAGGIFHPLGLALVSRKPVVVLDPYRGRVWSASEEAQKTLRKRLLKIYEAK
CCCCCEEEEEECCHHHHHHHHHHCCCCEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHH
ISGVRVGLIIGSRLGQYRPWLIEKLEREALEKGYRVYKVSSTYLSIERLIAIDNALNLDI
HCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCCEEE
YVVTSCPRLPIDDLDDFYKPVLTPGEFLMLLTGSEKYVYPW
EEEECCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]