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The map label for this gene is gutB [H]

Identifier: 218884707

GI number: 218884707

Start: 1298724

End: 1299815

Strand: Direct

Name: gutB [H]

Synonym: DKAM_1396

Alternate gene names: 218884707

Gene position: 1298724-1299815 (Clockwise)

Preceding gene: 218884702

Following gene: 218884708

Centisome position: 95.13

GC content: 49.36

Gene sequence:

>1092_bases
ATGAAAACAAATATGTTTCTTAGATACATTACAACTGAAAGCCCGCCCTTTAGGGCGGGAAGAAGGTTAGAGCATGTGTG
GGGATACAGTGTGAAGGCTCTTCTACTTCTCGGAGCAAGAGATCTGAGGCTTGAAGAAGTTGAAGAACCAAGACCTGCTC
CAGAATGGGTCCTCATCAAAGTTGAGAGGGTCGGTATCTGTGGAACAGATAAGGCCATGTACTCTGGGACATATAAGCTA
CTCAAGAAGCCCCTTATCCCGGGTCACGAGGTTTCAGGTATAGTTGTCGATGTTGGCGAGGGGGTTTCAAGGGATCTCGT
AGGGCACAGGGTTACAACCGAGATAAATGTCTACTGTGGTAGGTGCTGGTATTGTAGAAGTGGAATGTATACTCACTGCC
CCTACAGGGAGACCATAGGGATAACACGTAACGGGGGTATGGCAGAATACATGGTGACAAGACGGGATCTCATCCATGTA
GTCGATGACTTAACACCGCTTCAGGCGGCATTTATTGAGCCGCTTGCGGCAGTGGTCGAGATGATTGAGATGGAACCGGT
TAAACCATTAAGCAGAGTTGCAGTTATAGGTGTTGGTGCAATTGGATTACTCGCCATACAAGTGCTCAAACTATTCGAGC
CGGAGCTCCTGGTAGCTGTCTCGAGGCCCGGCTCTCCGAAGGCTCGTCTAGCCAGGGAGCTTGGAGCAGATTTAGTCGTA
GATTACCAAGGGCTTAGTGAAGTAGTTAAGAAGGATACCCCAGAGGGACAGGGGTTCGACTACGTCGTTGAGGCCACTGG
TAGCTCCAGCGGTTTAGAAGTAGCGCTTGACGTAGCTAGGCCGAGAGGTGTTGTCGCGTTGAAGTCAACCCATGGCTCCC
CGGTAAGCTTTGATCAGACCAAGGCTGTTGTAAAGGAGTTAAGGCTATCGACGTCCAGGTGCGGTCCCTTCGATAAAGCG
ATAAGCCTGTTGAGGAAAAGAATAGTGGTTGTCGATAAACTAGTTACCTCAGAATATCCGTTGACAAGGGGTGTTGAAGC
CTTCGAGAAGAGTTTTGAGAGAGACCAGGTGAAAATACACCTGGTCACCTAG

Upstream 100 bases:

>100_bases
ATGATGACTACTATTATAACTAATACAAGGGTTCTCATAGCCAGGGATCTCATCAGTTCACCCCTTATTGATAATTTAAA
TATCGATGATATATAAAAAT

Downstream 100 bases:

>100_bases
GTTTATTTTTATGTTTGAATAATTATTTTAGGAGGGATGAATCCATGCGTGTAGCGGTTGGATCAGATGACGTATATAGT
GTTGCAAGATTCCTCGTGGA

Product: alcohol dehydrogenase GroES domain-containing protein

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKTNMFLRYITTESPPFRAGRRLEHVWGYSVKALLLLGARDLRLEEVEEPRPAPEWVLIKVERVGICGTDKAMYSGTYKL
LKKPLIPGHEVSGIVVDVGEGVSRDLVGHRVTTEINVYCGRCWYCRSGMYTHCPYRETIGITRNGGMAEYMVTRRDLIHV
VDDLTPLQAAFIEPLAAVVEMIEMEPVKPLSRVAVIGVGAIGLLAIQVLKLFEPELLVAVSRPGSPKARLARELGADLVV
DYQGLSEVVKKDTPEGQGFDYVVEATGSSSGLEVALDVARPRGVVALKSTHGSPVSFDQTKAVVKELRLSTSRCGPFDKA
ISLLRKRIVVVDKLVTSEYPLTRGVEAFEKSFERDQVKIHLVT

Sequences:

>Translated_363_residues
MKTNMFLRYITTESPPFRAGRRLEHVWGYSVKALLLLGARDLRLEEVEEPRPAPEWVLIKVERVGICGTDKAMYSGTYKL
LKKPLIPGHEVSGIVVDVGEGVSRDLVGHRVTTEINVYCGRCWYCRSGMYTHCPYRETIGITRNGGMAEYMVTRRDLIHV
VDDLTPLQAAFIEPLAAVVEMIEMEPVKPLSRVAVIGVGAIGLLAIQVLKLFEPELLVAVSRPGSPKARLARELGADLVV
DYQGLSEVVKKDTPEGQGFDYVVEATGSSSGLEVALDVARPRGVVALKSTHGSPVSFDQTKAVVKELRLSTSRCGPFDKA
ISLLRKRIVVVDKLVTSEYPLTRGVEAFEKSFERDQVKIHLVT
>Mature_363_residues
MKTNMFLRYITTESPPFRAGRRLEHVWGYSVKALLLLGARDLRLEEVEEPRPAPEWVLIKVERVGICGTDKAMYSGTYKL
LKKPLIPGHEVSGIVVDVGEGVSRDLVGHRVTTEINVYCGRCWYCRSGMYTHCPYRETIGITRNGGMAEYMVTRRDLIHV
VDDLTPLQAAFIEPLAAVVEMIEMEPVKPLSRVAVIGVGAIGLLAIQVLKLFEPELLVAVSRPGSPKARLARELGADLVV
DYQGLSEVVKKDTPEGQGFDYVVEATGSSSGLEVALDVARPRGVVALKSTHGSPVSFDQTKAVVKELRLSTSRCGPFDKA
ISLLRKRIVVVDKLVTSEYPLTRGVEAFEKSFERDQVKIHLVT

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=340, Percent_Identity=27.9411764705882, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI4501929, Length=258, Percent_Identity=29.4573643410853, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI34577061, Length=258, Percent_Identity=27.5193798449612, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI156523966, Length=261, Percent_Identity=26.8199233716475, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI4501939, Length=261, Percent_Identity=26.8199233716475, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI4501933, Length=258, Percent_Identity=26.7441860465116, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI71565154, Length=272, Percent_Identity=25.7352941176471, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1787863, Length=336, Percent_Identity=28.8690476190476, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI226510992, Length=325, Percent_Identity=26.1538461538462, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788073, Length=236, Percent_Identity=31.3559322033898, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788075, Length=341, Percent_Identity=25.8064516129032, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790045, Length=285, Percent_Identity=29.4736842105263, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790718, Length=319, Percent_Identity=28.8401253918495, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788407, Length=288, Percent_Identity=27.0833333333333, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082125, Length=271, Percent_Identity=29.520295202952, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081918, Length=187, Percent_Identity=31.5508021390374, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=341, Percent_Identity=30.4985337243402, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17562878, Length=341, Percent_Identity=28.1524926686217, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17562584, Length=298, Percent_Identity=28.1879194630872, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17562582, Length=299, Percent_Identity=26.4214046822742, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988145, Length=172, Percent_Identity=31.9767441860465, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319257, Length=361, Percent_Identity=27.4238227146814, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323099, Length=336, Percent_Identity=27.3809523809524, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319621, Length=266, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319955, Length=339, Percent_Identity=25.3687315634218, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6322619, Length=339, Percent_Identity=25.3687315634218, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=288, Percent_Identity=27.4305555555556, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323729, Length=254, Percent_Identity=26.3779527559055, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6319258, Length=359, Percent_Identity=26.4623955431755, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324486, Length=283, Percent_Identity=25.4416961130742, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=280, Percent_Identity=25, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137530, Length=331, Percent_Identity=28.3987915407855, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17737897, Length=345, Percent_Identity=29.5652173913043, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI45550770, Length=325, Percent_Identity=25.8461538461538, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45551930, Length=325, Percent_Identity=25.8461538461538, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI221457811, Length=325, Percent_Identity=25.8461538461538, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17737895, Length=354, Percent_Identity=25.7062146892655, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 40116; Mature: 40116

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNMFLRYITTESPPFRAGRRLEHVWGYSVKALLLLGARDLRLEEVEEPRPAPEWVLIK
CCCCEEEEEEECCCCCCCCCCHHHHHHCHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEE
VERVGICGTDKAMYSGTYKLLKKPLIPGHEVSGIVVDVGEGVSRDLVGHRVTTEINVYCG
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCEEEEEEEEEEC
RCWYCRSGMYTHCPYRETIGITRNGGMAEYMVTRRDLIHVVDDLTPLQAAFIEPLAAVVE
EEEEECCCCEECCCCHHHCCEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
MIEMEPVKPLSRVAVIGVGAIGLLAIQVLKLFEPELLVAVSRPGSPKARLARELGADLVV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCEEE
DYQGLSEVVKKDTPEGQGFDYVVEATGSSSGLEVALDVARPRGVVALKSTHGSPVSFDQT
EHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHH
KAVVKELRLSTSRCGPFDKAISLLRKRIVVVDKLVTSEYPLTRGVEAFEKSFERDQVKIH
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEE
LVT
EEC
>Mature Secondary Structure
MKTNMFLRYITTESPPFRAGRRLEHVWGYSVKALLLLGARDLRLEEVEEPRPAPEWVLIK
CCCCEEEEEEECCCCCCCCCCHHHHHHCHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEE
VERVGICGTDKAMYSGTYKLLKKPLIPGHEVSGIVVDVGEGVSRDLVGHRVTTEINVYCG
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCEEEEEEEEEEC
RCWYCRSGMYTHCPYRETIGITRNGGMAEYMVTRRDLIHVVDDLTPLQAAFIEPLAAVVE
EEEEECCCCEECCCCHHHCCEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
MIEMEPVKPLSRVAVIGVGAIGLLAIQVLKLFEPELLVAVSRPGSPKARLARELGADLVV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCEEE
DYQGLSEVVKKDTPEGQGFDYVVEATGSSSGLEVALDVARPRGVVALKSTHGSPVSFDQT
EHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHH
KAVVKELRLSTSRCGPFDKAISLLRKRIVVVDKLVTSEYPLTRGVEAFEKSFERDQVKIH
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEE
LVT
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]