Click here to switch to the map view.

The map label for this gene is rpiB [H]

Identifier: 218884708

GI number: 218884708

Start: 1299830

End: 1300300

Strand: Direct

Name: rpiB [H]

Synonym: DKAM_1397

Alternate gene names: 218884708

Gene position: 1299830-1300300 (Clockwise)

Preceding gene: 218884707

Following gene: 218884710

Centisome position: 95.21

GC content: 47.35

Gene sequence:

>471_bases
TTGAATAATTATTTTAGGAGGGATGAATCCATGCGTGTAGCGGTTGGATCAGATGACGTATATAGTGTTGCAAGATTCCT
CGTGGACTACCTGAGGAAGAAGGGATTCGAGGTTAAACTAGTCGGCGCCCTCGCGACGGGAAAGCCGTATCCATGGCCCG
ATGTAGGCTTTGAGGTGGGCTCTATGGTGGCTAGAGGCGAGGTAGATTATGGCATTGTTATATGTTACACTGGTACAGGT
GTATCAATAGCAGCCAATAAGATCAAGGGAGTTAGGGCAGCACTCGTCACAGATGCCAGGAACGCTGAGGGAGCTAGGAG
ATGGAATGATGCCAATGTCCTCGCGTTAAGTGCCAGGTTAACTAGTGAGGAGCTGGCTAAGGAGATAGTGGATGCGTTCT
TCAATACTAGGGAAATAGATTCATCTGAGGTAGAGAACATAGAGAAGCTGAAGGCTATTGATAATGCTTGA

Upstream 100 bases:

>100_bases
CCTCAGAATATCCGTTGACAAGGGGTGTTGAAGCCTTCGAGAAGAGTTTTGAGAGAGACCAGGTGAAAATACACCTGGTC
ACCTAGGTTTATTTTTATGT

Downstream 100 bases:

>100_bases
GGGTAAAGTCATCGAGATCGTTGAGGAATCGATGATGACTTAAGAGCTATTCCACTGTAACGGTTTTAGCCAGGTTTCTC
GGCTTATCCGGGTCAAGGCC

Product: RpiB/LacA/LacB family sugar-phosphate isomerase

Products: NA

Alternate protein names: Phosphoriboisomerase B [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MNNYFRRDESMRVAVGSDDVYSVARFLVDYLRKKGFEVKLVGALATGKPYPWPDVGFEVGSMVARGEVDYGIVICYTGTG
VSIAANKIKGVRAALVTDARNAEGARRWNDANVLALSARLTSEELAKEIVDAFFNTREIDSSEVENIEKLKAIDNA

Sequences:

>Translated_156_residues
MNNYFRRDESMRVAVGSDDVYSVARFLVDYLRKKGFEVKLVGALATGKPYPWPDVGFEVGSMVARGEVDYGIVICYTGTG
VSIAANKIKGVRAALVTDARNAEGARRWNDANVLALSARLTSEELAKEIVDAFFNTREIDSSEVENIEKLKAIDNA
>Mature_156_residues
MNNYFRRDESMRVAVGSDDVYSVARFLVDYLRKKGFEVKLVGALATGKPYPWPDVGFEVGSMVARGEVDYGIVICYTGTG
VSIAANKIKGVRAALVTDARNAEGARRWNDANVLALSARLTSEELAKEIVDAFFNTREIDSSEVENIEKLKAIDNA

Specific function: In addition to its activity on D-ribose 5-phosphate it probably also has activity on D-allose 6-phosphate [H]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=145, Percent_Identity=37.2413793103448, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: =5.3.1.6 [H]

Molecular weight: Translated: 17158; Mature: 17158

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNYFRRDESMRVAVGSDDVYSVARFLVDYLRKKGFEVKLVGALATGKPYPWPDVGFEVG
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHH
SMVARGEVDYGIVICYTGTGVSIAANKIKGVRAALVTDARNAEGARRWNDANVLALSARL
HEHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECC
TSEELAKEIVDAFFNTREIDSSEVENIEKLKAIDNA
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNYFRRDESMRVAVGSDDVYSVARFLVDYLRKKGFEVKLVGALATGKPYPWPDVGFEVG
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHH
SMVARGEVDYGIVICYTGTGVSIAANKIKGVRAALVTDARNAEGARRWNDANVLALSARL
HEHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECC
TSEELAKEIVDAFFNTREIDSSEVENIEKLKAIDNA
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8576032; 7610040; 9278503; 1840580; 7984428; 9401019 [H]