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The map label for this gene is pelA

Identifier: 218884607

GI number: 218884607

Start: 1214294

End: 1215364

Strand: Direct

Name: pelA

Synonym: DKAM_1296

Alternate gene names: 218884607

Gene position: 1214294-1215364 (Clockwise)

Preceding gene: 218884600

Following gene: 218884609

Centisome position: 88.94

GC content: 47.06

Gene sequence:

>1071_bases
ATGAGGGTACTGGAGGAGGACTTGAGAAATGGATACATAAGGATCCTAGTGGAGGACATCGATGACTTATGGATATTATT
CATGGTTCTTAGAAAGGGGGATATAGTCTACGCTAGAACGAGCAGGGAGGTTAAGCCGGGTGAGGGTGGCAGTAGTAGAA
GGATACCGATGGTTCTCGGGCTACGCGTCGAGGCAATTGAATTCCAGGAGTTCACGGAGAAGCTGAGGATCAGAGGTATT
GTTGTGGAGGGGCCAGAGGAATTCGGGGTAAAGGGTCACTATCACACGATCGCTATTGGTGTGGGAGACCAGTTAGCCAT
AGTGAGAGAAACCTGGAGTAAGCATAGTCTAGACATCTTGAAGAAGGGGGTTAGAAGGAGGAGAATACTATTAGTGTCAA
TAGACTATGATAGTGCATGCATAGCCGTTTTAACCGAGCAAGGAGTGAAACACCATAGTGAGATACAGTCAGATCTCCCG
GGAAAAATGTATAGGGTTGAACACGAGGAAATATTAGACGAATACCTAAGTAAGGTAGCCTCAGCATTAAATAATGTTAT
ACAGCAGGAGGAAATAGATGCAGTGATAATTGCTGGACCAGGCGACTTGAAGAACAAGCTCGGTGAAAACATAAGGAGAG
ACAACAGGAAACATGTATACCTGGATACAACCTCTACAGGGGGATGCCAGGGGATTTCAGAGCTACTTGGCAGGGATGTG
GTTAAACAGGTTGTCGGGGATTTAAGTATTGTTAAGGCTAAAGAAGTAGTTGAGGAGTTTAAGAGGCTCATTATAAAGGA
TCCTCAGCTCGTAGCATACGGCGTGGATGATGTATACGGGGCGGTAGTGTACGCAGCTGTCTCAAGAATTGTTGTAGCCG
GTGACCTACTTCACGAGCCCGATGATGAAAGGCGCGCCCGCGTATACGAGATCCTTGAGAAAGCATATGAAACCGGTGCA
GAGGTGATAATAGTCCCCGGGAAAAGCGATGTAGGCCTCGAGGTCCAAGGGTTCGGCGGGGTAATAGCAGTGCTCAGGTA
TAGGTTATTTCGTGGAGAGCATTCTCCTTAA

Upstream 100 bases:

>100_bases
TTGTTTTTTCACGCTTCAATAATATGATATCTTAATACGCTCATAAAGCTATTACTATAGAGAAAGCATGCAGATGCTGT
GTCAGCTGAAGGTGTAGTTG

Downstream 100 bases:

>100_bases
CACGCCATTCTCTCGCGATAGCTCTCTCAACGCGTCCTCGAATGTTTCATCGGATGCCAGGTAATACTTGATGAAGACAC
CTGTTCTCCCTATACTGCTT

Product: putative translation factor Pelota-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MRVLEEDLRNGYIRILVEDIDDLWILFMVLRKGDIVYARTSREVKPGEGGSSRRIPMVLGLRVEAIEFQEFTEKLRIRGI
VVEGPEEFGVKGHYHTIAIGVGDQLAIVRETWSKHSLDILKKGVRRRRILLVSIDYDSACIAVLTEQGVKHHSEIQSDLP
GKMYRVEHEEILDEYLSKVASALNNVIQQEEIDAVIIAGPGDLKNKLGENIRRDNRKHVYLDTTSTGGCQGISELLGRDV
VKQVVGDLSIVKAKEVVEEFKRLIIKDPQLVAYGVDDVYGAVVYAAVSRIVVAGDLLHEPDDERRARVYEILEKAYETGA
EVIIVPGKSDVGLEVQGFGGVIAVLRYRLFRGEHSP

Sequences:

>Translated_356_residues
MRVLEEDLRNGYIRILVEDIDDLWILFMVLRKGDIVYARTSREVKPGEGGSSRRIPMVLGLRVEAIEFQEFTEKLRIRGI
VVEGPEEFGVKGHYHTIAIGVGDQLAIVRETWSKHSLDILKKGVRRRRILLVSIDYDSACIAVLTEQGVKHHSEIQSDLP
GKMYRVEHEEILDEYLSKVASALNNVIQQEEIDAVIIAGPGDLKNKLGENIRRDNRKHVYLDTTSTGGCQGISELLGRDV
VKQVVGDLSIVKAKEVVEEFKRLIIKDPQLVAYGVDDVYGAVVYAAVSRIVVAGDLLHEPDDERRARVYEILEKAYETGA
EVIIVPGKSDVGLEVQGFGGVIAVLRYRLFRGEHSP
>Mature_356_residues
MRVLEEDLRNGYIRILVEDIDDLWILFMVLRKGDIVYARTSREVKPGEGGSSRRIPMVLGLRVEAIEFQEFTEKLRIRGI
VVEGPEEFGVKGHYHTIAIGVGDQLAIVRETWSKHSLDILKKGVRRRRILLVSIDYDSACIAVLTEQGVKHHSEIQSDLP
GKMYRVEHEEILDEYLSKVASALNNVIQQEEIDAVIIAGPGDLKNKLGENIRRDNRKHVYLDTTSTGGCQGISELLGRDV
VKQVVGDLSIVKAKEVVEEFKRLIIKDPQLVAYGVDDVYGAVVYAAVSRIVVAGDLLHEPDDERRARVYEILEKAYETGA
EVIIVPGKSDVGLEVQGFGGVIAVLRYRLFRGEHSP

Specific function: May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has end

COG id: COG1537

COG function: function code R; Predicted RNA-binding proteins

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family. Pelota subfamily

Homologues:

Organism=Homo sapiens, GI31880783, Length=364, Percent_Identity=20.6043956043956, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17554648, Length=360, Percent_Identity=22.7777777777778, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324327, Length=369, Percent_Identity=21.1382113821138, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136914, Length=368, Percent_Identity=22.2826086956522, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PELO_DESK1 (B8D691)

Other databases:

- EMBL:   CP001140
- RefSeq:   YP_002428989.1
- GeneID:   7171660
- GenomeReviews:   CP001140_GR
- KEGG:   dka:DKAM_1296
- HOGENOM:   HBG646058
- OMA:   MKLVRKD
- ProtClustDB:   CLSK2399603
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01853
- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR004405
- PANTHER:   PTHR10853
- TIGRFAMs:   TIGR00111

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3

EC number: NA

Molecular weight: Translated: 39906; Mature: 39906

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLEEDLRNGYIRILVEDIDDLWILFMVLRKGDIVYARTSREVKPGEGGSSRRIPMVLG
CCCCHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEC
LRVEAIEFQEFTEKLRIRGIVVEGPEEFGVKGHYHTIAIGVGDQLAIVRETWSKHSLDIL
CEEEEHHHHHHHHHHHEEEEEEECCHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHH
KKGVRRRRILLVSIDYDSACIAVLTEQGVKHHSEIQSDLPGKMYRVEHEEILDEYLSKVA
HHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHH
SALNNVIQQEEIDAVIIAGPGDLKNKLGENIRRDNRKHVYLDTTSTGGCQGISELLGRDV
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
VKQVVGDLSIVKAKEVVEEFKRLIIKDPQLVAYGVDDVYGAVVYAAVSRIVVAGDLLHEP
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCC
DDERRARVYEILEKAYETGAEVIIVPGKSDVGLEVQGFGGVIAVLRYRLFRGEHSP
CHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRVLEEDLRNGYIRILVEDIDDLWILFMVLRKGDIVYARTSREVKPGEGGSSRRIPMVLG
CCCCHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEC
LRVEAIEFQEFTEKLRIRGIVVEGPEEFGVKGHYHTIAIGVGDQLAIVRETWSKHSLDIL
CEEEEHHHHHHHHHHHEEEEEEECCHHHCCCCCEEEEEEECCCCHHHHHHHHCCCCHHHH
KKGVRRRRILLVSIDYDSACIAVLTEQGVKHHSEIQSDLPGKMYRVEHEEILDEYLSKVA
HHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHH
SALNNVIQQEEIDAVIIAGPGDLKNKLGENIRRDNRKHVYLDTTSTGGCQGISELLGRDV
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
VKQVVGDLSIVKAKEVVEEFKRLIIKDPQLVAYGVDDVYGAVVYAAVSRIVVAGDLLHEP
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCC
DDERRARVYEILEKAYETGAEVIIVPGKSDVGLEVQGFGGVIAVLRYRLFRGEHSP
CHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA