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The map label for this gene is hyfB [H]

Identifier: 218884329

GI number: 218884329

Start: 968029

End: 969537

Strand: Direct

Name: hyfB [H]

Synonym: DKAM_1018

Alternate gene names: 218884329

Gene position: 968029-969537 (Clockwise)

Preceding gene: 218884328

Following gene: 218884330

Centisome position: 70.91

GC content: 41.75

Gene sequence:

>1509_bases
ATGATTCACGGTATAATCACCTCGGCATCATACATACTCGTGTTAACAGGATCAGCGACAAGTGATAGCCTGACCAGGCT
TAGCAGGGTGCTCAGAGTAATAGGTTTCGCTCTCCCATTAATCGATGCATTATTCAACCCAGCTGGGTTACTTGACTTTC
AGAAATACTTCGTAGTGCTTGCAACACTTGTATCAGTGGCTGTAACCCTGCACTCGGAGGGGTACTATAGGGTAATTTAT
GGAAAAGTATCATATCAGCAATCACTATTAAATACCGTGCTTGTAACAGTGCTCACAGTGTTCAACGCCAGATTACTCGG
GGAATTTGTCATTGGGCTGCTACTACTAGACCTGCTACTGATACTAGTGATTCTAATGGATAAGGGGGCTGAAAACTATG
GGATAGCAATATACTATATAGTGTTAAGCACTATACCAGCCGACATGGCTGTTCTAACAATGTGGGCAACAATAGCACAG
AACTATGGTTTAGAGGCGTCGCTGACAACGAGTTTTTCAACGCTAGCACTACACAAGATCAGTATACACCCATTGGTGGC
AACATTGATATTGATCGGGTACACTATAAAACTAGGGTTATTCCCACTACACATATGGCTACCCATAGTACATCCAGAGG
CACCTAAACACGGCTCAGCAATACTGAGCGGATTAATAATAAAGATAGGTGTCTTCGGGTTGCTTCTAGCCTCGATGATA
TTCACATATCCCGGGTACATGAATTACATCATATTAGTACAGGGAGCGATAACGGCTTTATACGGGAGCTTCGCCGCGAC
ACTTCAGAAAGATATTAGAAGACTACTGGCATATAGCTCGCTAGGGCATGCCGGAGTAATAGCCATACTATACTCGTTTT
CAATACTCTATGGAGACCCCCTTATATCATATCTTTTATACTTCTATATAATATACCATGGAGTAGTTAAGGCCCATGCA
TTCATGAATGCCGCGTTAATGGATCAATTAGCTAACACCTATGACATTTATAAACTAGGGTACCTTGGACAGTTATACCC
GTCTTTAATCATTCCAGCCTTTAATATATTCATGAACTTCGCCGGAATACCGCCCTCTTACGGCTTCATGATGAAGCTAA
TGCTTCTAATAACAATTATACTGCTCATACCGTCACTAGGGATTATACCATTACTTACAAGCATAATTATCATTGTAGTA
GCCGTGTTCTCGATACTCTACAGTATTAAGATAATTGGATGCTATATAGGGGGCTACAGAAAAGTCGTAAAACCCCCTGT
AAAAGTCATGGAAGAGGAGTTATTCTCCGAGTACTATATCGCGTTCATCACGCTCATTCTCCCCATCATATACATAGCAT
TATCCATGGAGTACATGCCCGGCTACCTAGCTATTACACTACTGTTACTAGAAGCAATCTCCCTACTCATGTATACATGG
ATACTGTTTAGGAAGCCCATATATAAGACAGGTGAACCAAAATACTGGCTCTCAGGTGTTGAGCAATGA

Upstream 100 bases:

>100_bases
ATTTAATCCTCCTGTTTTCAATGGTGTCACCCCTGATGCTTCTCGCTACGGTACTCATGTACCTCACAGTGGCAAGAGAG
AGGGGTGTTAAGCATTGAGT

Downstream 100 bases:

>100_bases
GCTACATAAGGGCTAGCATAGTAAGATGGTTTGAAGAAATAGCTGAGAAGACCATGGAGAAGCTTAAATCAACACACAAG
GGCACACTCCCAACGTTGAT

Product: NADH/ubiquinone/plastoquinone (complex I)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MIHGIITSASYILVLTGSATSDSLTRLSRVLRVIGFALPLIDALFNPAGLLDFQKYFVVLATLVSVAVTLHSEGYYRVIY
GKVSYQQSLLNTVLVTVLTVFNARLLGEFVIGLLLLDLLLILVILMDKGAENYGIAIYYIVLSTIPADMAVLTMWATIAQ
NYGLEASLTTSFSTLALHKISIHPLVATLILIGYTIKLGLFPLHIWLPIVHPEAPKHGSAILSGLIIKIGVFGLLLASMI
FTYPGYMNYIILVQGAITALYGSFAATLQKDIRRLLAYSSLGHAGVIAILYSFSILYGDPLISYLLYFYIIYHGVVKAHA
FMNAALMDQLANTYDIYKLGYLGQLYPSLIIPAFNIFMNFAGIPPSYGFMMKLMLLITIILLIPSLGIIPLLTSIIIIVV
AVFSILYSIKIIGCYIGGYRKVVKPPVKVMEEELFSEYYIAFITLILPIIYIALSMEYMPGYLAITLLLLEAISLLMYTW
ILFRKPIYKTGEPKYWLSGVEQ

Sequences:

>Translated_502_residues
MIHGIITSASYILVLTGSATSDSLTRLSRVLRVIGFALPLIDALFNPAGLLDFQKYFVVLATLVSVAVTLHSEGYYRVIY
GKVSYQQSLLNTVLVTVLTVFNARLLGEFVIGLLLLDLLLILVILMDKGAENYGIAIYYIVLSTIPADMAVLTMWATIAQ
NYGLEASLTTSFSTLALHKISIHPLVATLILIGYTIKLGLFPLHIWLPIVHPEAPKHGSAILSGLIIKIGVFGLLLASMI
FTYPGYMNYIILVQGAITALYGSFAATLQKDIRRLLAYSSLGHAGVIAILYSFSILYGDPLISYLLYFYIIYHGVVKAHA
FMNAALMDQLANTYDIYKLGYLGQLYPSLIIPAFNIFMNFAGIPPSYGFMMKLMLLITIILLIPSLGIIPLLTSIIIIVV
AVFSILYSIKIIGCYIGGYRKVVKPPVKVMEEELFSEYYIAFITLILPIIYIALSMEYMPGYLAITLLLLEAISLLMYTW
ILFRKPIYKTGEPKYWLSGVEQ
>Mature_502_residues
MIHGIITSASYILVLTGSATSDSLTRLSRVLRVIGFALPLIDALFNPAGLLDFQKYFVVLATLVSVAVTLHSEGYYRVIY
GKVSYQQSLLNTVLVTVLTVFNARLLGEFVIGLLLLDLLLILVILMDKGAENYGIAIYYIVLSTIPADMAVLTMWATIAQ
NYGLEASLTTSFSTLALHKISIHPLVATLILIGYTIKLGLFPLHIWLPIVHPEAPKHGSAILSGLIIKIGVFGLLLASMI
FTYPGYMNYIILVQGAITALYGSFAATLQKDIRRLLAYSSLGHAGVIAILYSFSILYGDPLISYLLYFYIIYHGVVKAHA
FMNAALMDQLANTYDIYKLGYLGQLYPSLIIPAFNIFMNFAGIPPSYGFMMKLMLLITIILLIPSLGIIPLLTSIIIIVV
AVFSILYSIKIIGCYIGGYRKVVKPPVKVMEEELFSEYYIAFITLILPIIYIALSMEYMPGYLAITLLLLEAISLLMYTW
ILFRKPIYKTGEPKYWLSGVEQ

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788827, Length=214, Percent_Identity=31.7757009345794, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788613, Length=223, Percent_Identity=29.1479820627803, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788831, Length=188, Percent_Identity=28.7234042553192, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 55680; Mature: 55680

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHGIITSASYILVLTGSATSDSLTRLSRVLRVIGFALPLIDALFNPAGLLDFQKYFVVL
CCCCCEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ATLVSVAVTLHSEGYYRVIYGKVSYQQSLLNTVLVTVLTVFNARLLGEFVIGLLLLDLLL
HHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILVILMDKGAENYGIAIYYIVLSTIPADMAVLTMWATIAQNYGLEASLTTSFSTLALHKI
HHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEHHCCHHHHHHHHH
SIHPLVATLILIGYTIKLGLFPLHIWLPIVHPEAPKHGSAILSGLIIKIGVFGLLLASMI
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTYPGYMNYIILVQGAITALYGSFAATLQKDIRRLLAYSSLGHAGVIAILYSFSILYGDP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
LISYLLYFYIIYHGVVKAHAFMNAALMDQLANTYDIYKLGYLGQLYPSLIIPAFNIFMNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIPPSYGFMMKLMLLITIILLIPSLGIIPLLTSIIIIVVAVFSILYSIKIIGCYIGGYR
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KVVKPPVKVMEEELFSEYYIAFITLILPIIYIALSMEYMPGYLAITLLLLEAISLLMYTW
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ILFRKPIYKTGEPKYWLSGVEQ
HHHHCCCCCCCCCCHHHCCCCC
>Mature Secondary Structure
MIHGIITSASYILVLTGSATSDSLTRLSRVLRVIGFALPLIDALFNPAGLLDFQKYFVVL
CCCCCEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ATLVSVAVTLHSEGYYRVIYGKVSYQQSLLNTVLVTVLTVFNARLLGEFVIGLLLLDLLL
HHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILVILMDKGAENYGIAIYYIVLSTIPADMAVLTMWATIAQNYGLEASLTTSFSTLALHKI
HHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEHHCCHHHHHHHHH
SIHPLVATLILIGYTIKLGLFPLHIWLPIVHPEAPKHGSAILSGLIIKIGVFGLLLASMI
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTYPGYMNYIILVQGAITALYGSFAATLQKDIRRLLAYSSLGHAGVIAILYSFSILYGDP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
LISYLLYFYIIYHGVVKAHAFMNAALMDQLANTYDIYKLGYLGQLYPSLIIPAFNIFMNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIPPSYGFMMKLMLLITIILLIPSLGIIPLLTSIIIIVVAVFSILYSIKIIGCYIGGYR
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
KVVKPPVKVMEEELFSEYYIAFITLILPIIYIALSMEYMPGYLAITLLLLEAISLLMYTW
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ILFRKPIYKTGEPKYWLSGVEQ
HHHHCCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]