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The map label for this gene is phsB [H]

Identifier: 218884327

GI number: 218884327

Start: 965989

End: 966489

Strand: Direct

Name: phsB [H]

Synonym: DKAM_1016

Alternate gene names: 218884327

Gene position: 965989-966489 (Clockwise)

Preceding gene: 218884326

Following gene: 218884328

Centisome position: 70.76

GC content: 50.5

Gene sequence:

>501_bases
GTGACCTATATGAGTTCGAACCCGGGTAGATACTACAGGATAATAGACCTAGGGACATGCATAGGATGCGGCGCATGCGA
GGCCGCATGCGATTTCATACATGATGGGCACCCATATATAAAGGTATATAGGACGGAACTAGGCATTGAGATACCCGTTT
CATGCATGCACTGCGCTAAAGCTCCATGTATAGATGTCTGCCCAACAGGCGCGATGACCAGGGATAGTGAGGGAGCCGTG
TACGTTGTAGCAAGCAAGTGCATCGGGTGCATGGCATGCCTATATGCATGCCCATTCGGCATCCCAGAGCTCGATAAATC
ACTAAGGGTATCAACTAAGTGCGACCTCTGCATGGATCAAAGGAGGAAAGGATTGGAGCCAGGCTGTAGCTCTATATGTC
CAGCCGGGGCAATAATATACGGGGAGGAAGCATTGGTATTCGATAAGGCGAAGAAGAAGGCAGCTGAAGCTATGGCGAGG
GCAAGGTTCGAGACACTGTAG

Upstream 100 bases:

>100_bases
CAGGCCCCTCGAAGGTGGGCCCAGCAATAAAGAACGGCCTATACACAGCCAAATACATGCACAACTGGATAGAGGCTAAA
CTAGTTGCTTCAATAGCGAG

Downstream 100 bases:

>100_bases
GTGAAGCTATGTGAGCTATGTGAACACGCTAGCCACTAGTATACTGCTCTATCTTTTCTCACTTGTAATACTCATATTCA
TAAGTAGACCATATTTAAAT

Product: 4Fe-4S ferredoxin, iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MTYMSSNPGRYYRIIDLGTCIGCGACEAACDFIHDGHPYIKVYRTELGIEIPVSCMHCAKAPCIDVCPTGAMTRDSEGAV
YVVASKCIGCMACLYACPFGIPELDKSLRVSTKCDLCMDQRRKGLEPGCSSICPAGAIIYGEEALVFDKAKKKAAEAMAR
ARFETL

Sequences:

>Translated_166_residues
MTYMSSNPGRYYRIIDLGTCIGCGACEAACDFIHDGHPYIKVYRTELGIEIPVSCMHCAKAPCIDVCPTGAMTRDSEGAV
YVVASKCIGCMACLYACPFGIPELDKSLRVSTKCDLCMDQRRKGLEPGCSSICPAGAIIYGEEALVFDKAKKKAAEAMAR
ARFETL
>Mature_165_residues
TYMSSNPGRYYRIIDLGTCIGCGACEAACDFIHDGHPYIKVYRTELGIEIPVSCMHCAKAPCIDVCPTGAMTRDSEGAVY
VVASKCIGCMACLYACPFGIPELDKSLRVSTKCDLCMDQRRKGLEPGCSSICPAGAIIYGEEALVFDKAKKKAAEAMARA
RFETL

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI226510944, Length=150, Percent_Identity=41.3333333333333, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1787749, Length=176, Percent_Identity=32.9545454545455, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI2367345, Length=152, Percent_Identity=35.5263157894737, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI1787122, Length=177, Percent_Identity=32.2033898305085, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790326, Length=176, Percent_Identity=30.6818181818182, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787872, Length=173, Percent_Identity=31.7919075144509, Blast_Score=84, Evalue=4e-18,
Organism=Escherichia coli, GI87082179, Length=138, Percent_Identity=39.8550724637681, Blast_Score=84, Evalue=4e-18,
Organism=Escherichia coli, GI1789370, Length=185, Percent_Identity=31.3513513513514, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87082180, Length=153, Percent_Identity=36.6013071895425, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788811, Length=136, Percent_Identity=36.7647058823529, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1789067, Length=180, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI2367245, Length=141, Percent_Identity=35.4609929078014, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1789079, Length=181, Percent_Identity=32.5966850828729, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI87082114, Length=86, Percent_Identity=40.6976744186046, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1787963, Length=140, Percent_Identity=30.7142857142857, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17946; Mature: 17815

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
13.9 %Cys+Met (Translated Protein)
9.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
13.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYMSSNPGRYYRIIDLGTCIGCGACEAACDFIHDGHPYIKVYRTELGIEIPVSCMHCAK
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCC
APCIDVCPTGAMTRDSEGAVYVVASKCIGCMACLYACPFGIPELDKSLRVSTKCDLCMDQ
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHH
RRKGLEPGCSSICPAGAIIYGEEALVFDKAKKKAAEAMARARFETL
HHCCCCCCCHHCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TYMSSNPGRYYRIIDLGTCIGCGACEAACDFIHDGHPYIKVYRTELGIEIPVSCMHCAK
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCC
APCIDVCPTGAMTRDSEGAVYVVASKCIGCMACLYACPFGIPELDKSLRVSTKCDLCMDQ
CCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHH
RRKGLEPGCSSICPAGAIIYGEEALVFDKAKKKAAEAMARARFETL
HHCCCCCCCHHCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]