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The map label for this gene is gltD [H]

Identifier: 218884326

GI number: 218884326

Start: 964901

End: 965992

Strand: Direct

Name: gltD [H]

Synonym: DKAM_1015

Alternate gene names: 218884326

Gene position: 964901-965992 (Clockwise)

Preceding gene: 218884321

Following gene: 218884327

Centisome position: 70.68

GC content: 49.36

Gene sequence:

>1092_bases
ATGATTGATTACATAAACTTCGCCTTCATGTGCAGGGAGAAGCAGAGCGATACAGGAATAAGGATAGCCATAGTAGGTGC
TGGACCAGCCGGGCTAGCAGCAACTGGATACCTGGCATGCCGGGGACACAGCATTGATGTATATGATAAGCAGCCTTACC
CAGGCGGATTAATGGTATTCGCTATACCACCCTGGAGAATACCGAGGAACAGGGTCTTAGAGGGCGTGAAAGACCTAGAG
AAGAGGCTGGGGGTTAGATTCATCACTAGAACCAAAGTATACGCCTCAGTAGAGATGCCTCACGAGGAGGGAGACGAGCT
AGTCGAGAAGAAGACCAGCCTAGAGGAGTTGATAAGCAACTACGATCTAGTGCTATTATCAACGGGGACATGGAGATCGA
AGATACCGAGGATCCAGGGGGCTAACGCTAAAGGTGTTGAAAGCGCCTTAGAATACGTGTATAGATTCAGACTCTACGAA
CTAGGACTAGTTAATTCAAAGCCTTCGGCAGGTAAGAAAGTAGTAGTTATAGGAGGCGGATACAGTGCCATAGACGCTGC
AGAACAGGCAGTAAAAGAAGGCGCCGAAGCCTACCTTGTATACAGGAGAACAATAAAGGAGGCTCCCGCAGGCATATTCG
AGGTAGAGAGGGTGAAGAGGCTGGGGGTAGAATTCATGGAGCTGGTTTCACCAGTAGAGATAATTAGCGAAGGCAACGCG
GTGAAAGCCATAAAGTTACAGAGAATGCAGCTCGGACCCCCAGATGAAACAGGGCGCCCCCAGCCAATCCCCGTGCCAGG
CTCGGAGATCATCATCGAGGCAGATAGAGTAATATTCGCCACGGGCGAGTTGCCGACACCCCCATTACCCCAGTCCGAGG
ACTACATGAATAAGCTAGGGATAAGGCTAAATAAGGATGGATCAATAGTAGTGAATAATATAATGCAGACAGGTAATCCA
AAAGTATTCGCGGCTGGAGACGTGGTAACAGGCCCCTCGAAGGTGGGCCCAGCAATAAAGAACGGCCTATACACAGCCAA
ATACATGCACAACTGGATAGAGGCTAAACTAGTTGCTTCAATAGCGAGGTGA

Upstream 100 bases:

>100_bases
TAAGCGTGGTAAGAATATTGTGGGAAATAAGTACCTATATATATGTTTTGAATAAATTTTAAAGACTCTATATACTTGAA
TACATCATAGAGGTGTAAAC

Downstream 100 bases:

>100_bases
CCTATATGAGTTCGAACCCGGGTAGATACTACAGGATAATAGACCTAGGGACATGCATAGGATGCGGCGCATGCGAGGCC
GCATGCGATTTCATACATGA

Product: putative glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MIDYINFAFMCREKQSDTGIRIAIVGAGPAGLAATGYLACRGHSIDVYDKQPYPGGLMVFAIPPWRIPRNRVLEGVKDLE
KRLGVRFITRTKVYASVEMPHEEGDELVEKKTSLEELISNYDLVLLSTGTWRSKIPRIQGANAKGVESALEYVYRFRLYE
LGLVNSKPSAGKKVVVIGGGYSAIDAAEQAVKEGAEAYLVYRRTIKEAPAGIFEVERVKRLGVEFMELVSPVEIISEGNA
VKAIKLQRMQLGPPDETGRPQPIPVPGSEIIIEADRVIFATGELPTPPLPQSEDYMNKLGIRLNKDGSIVVNNIMQTGNP
KVFAAGDVVTGPSKVGPAIKNGLYTAKYMHNWIEAKLVASIAR

Sequences:

>Translated_363_residues
MIDYINFAFMCREKQSDTGIRIAIVGAGPAGLAATGYLACRGHSIDVYDKQPYPGGLMVFAIPPWRIPRNRVLEGVKDLE
KRLGVRFITRTKVYASVEMPHEEGDELVEKKTSLEELISNYDLVLLSTGTWRSKIPRIQGANAKGVESALEYVYRFRLYE
LGLVNSKPSAGKKVVVIGGGYSAIDAAEQAVKEGAEAYLVYRRTIKEAPAGIFEVERVKRLGVEFMELVSPVEIISEGNA
VKAIKLQRMQLGPPDETGRPQPIPVPGSEIIIEADRVIFATGELPTPPLPQSEDYMNKLGIRLNKDGSIVVNNIMQTGNP
KVFAAGDVVTGPSKVGPAIKNGLYTAKYMHNWIEAKLVASIAR
>Mature_363_residues
MIDYINFAFMCREKQSDTGIRIAIVGAGPAGLAATGYLACRGHSIDVYDKQPYPGGLMVFAIPPWRIPRNRVLEGVKDLE
KRLGVRFITRTKVYASVEMPHEEGDELVEKKTSLEELISNYDLVLLSTGTWRSKIPRIQGANAKGVESALEYVYRFRLYE
LGLVNSKPSAGKKVVVIGGGYSAIDAAEQAVKEGAEAYLVYRRTIKEAPAGIFEVERVKRLGVEFMELVSPVEIISEGNA
VKAIKLQRMQLGPPDETGRPQPIPVPGSEIIIEADRVIFATGELPTPPLPQSEDYMNKLGIRLNKDGSIVVNNIMQTGNP
KVFAAGDVVTGPSKVGPAIKNGLYTAKYMHNWIEAKLVASIAR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=371, Percent_Identity=26.4150943396226, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789606, Length=348, Percent_Identity=34.1954022988506, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1788811, Length=351, Percent_Identity=34.1880341880342, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87082180, Length=350, Percent_Identity=30.5714285714286, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789243, Length=320, Percent_Identity=31.5625, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788468, Length=338, Percent_Identity=29.2899408284024, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17570289, Length=383, Percent_Identity=25.8485639686684, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17543860, Length=174, Percent_Identity=28.735632183908, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=356, Percent_Identity=26.4044943820225, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28574881, Length=360, Percent_Identity=30.8333333333333, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24665539, Length=360, Percent_Identity=30.8333333333333, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24665547, Length=360, Percent_Identity=30.8333333333333, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24665543, Length=360, Percent_Identity=30.8333333333333, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640763, Length=251, Percent_Identity=27.4900398406374, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI18858217, Length=251, Percent_Identity=27.4900398406374, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 39787; Mature: 39787

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYINFAFMCREKQSDTGIRIAIVGAGPAGLAATGYLACRGHSIDVYDKQPYPGGLMVF
CCCCEEEEEEEECCCCCCCEEEEEEECCCCCHHHCCEEEEECCEEEEEECCCCCCCEEEE
AIPPWRIPRNRVLEGVKDLEKRLGVRFITRTKVYASVEMPHEEGDELVEKKTSLEELISN
EECCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCC
YDLVLLSTGTWRSKIPRIQGANAKGVESALEYVYRFRLYELGLVNSKPSAGKKVVVIGGG
CCEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCC
YSAIDAAEQAVKEGAEAYLVYRRTIKEAPAGIFEVERVKRLGVEFMELVSPVEIISEGNA
CHHHHHHHHHHHCCHHEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCE
VKAIKLQRMQLGPPDETGRPQPIPVPGSEIIIEADRVIFATGELPTPPLPQSEDYMNKLG
EEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECEEEEEECCCCCCCCCCCHHHHHHCC
IRLNKDGSIVVNNIMQTGNPKVFAAGDVVTGPSKVGPAIKNGLYTAKYMHNWIEAKLVAS
CEECCCCCEEEEEHHHCCCCCEEECCCEECCCHHHCHHHHCCCCHHHHHHHHHHHHHHHH
IAR
HCC
>Mature Secondary Structure
MIDYINFAFMCREKQSDTGIRIAIVGAGPAGLAATGYLACRGHSIDVYDKQPYPGGLMVF
CCCCEEEEEEEECCCCCCCEEEEEEECCCCCHHHCCEEEEECCEEEEEECCCCCCCEEEE
AIPPWRIPRNRVLEGVKDLEKRLGVRFITRTKVYASVEMPHEEGDELVEKKTSLEELISN
EECCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCC
YDLVLLSTGTWRSKIPRIQGANAKGVESALEYVYRFRLYELGLVNSKPSAGKKVVVIGGG
CCEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEECCC
YSAIDAAEQAVKEGAEAYLVYRRTIKEAPAGIFEVERVKRLGVEFMELVSPVEIISEGNA
CHHHHHHHHHHHCCHHEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCE
VKAIKLQRMQLGPPDETGRPQPIPVPGSEIIIEADRVIFATGELPTPPLPQSEDYMNKLG
EEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECEEEEEECCCCCCCCCCCHHHHHHCC
IRLNKDGSIVVNNIMQTGNPKVFAAGDVVTGPSKVGPAIKNGLYTAKYMHNWIEAKLVAS
CEECCCCCEEEEEHHHCCCCCEEECCCEECCCHHHCHHHHCCCCHHHHHHHHHHHHHHHH
IAR
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]